The invention belongs to the field of product extraction technology and relates to a method for extracting
chitin and
antibacterial peptide from
fly larvae. The method comprises the following steps of: firstly disinfecting and rinsing fresh
fly larvae to be clean, then extruding the cleaned
fly larvae to separate the fly larvae
skin from visceral
mass serous fluid; firstly soaking with
hydrochloric acid solution,
drying after washing, and then soaking with NaOH solution to remove
protein; retaining residual liquid,
drying after cleaning, adding the NaOH solution after crushing, carrying out water bath at the temperature of 98 DEG C, and heating for reaction; putting
chitosan into
acetic acid solution, stopping reaction after the
chitosan is dissolved, filtering, cleaning,
drying at the temperature of 50 DEG C, and obtaining the
chitosan; and then adding
acetic acid into the visceral
mass serous fluid of the fly larvae, homogenizing, standing over the night at the temperature of 4 DEG C, filtering, adsorbing the filtrate with
alginic acid, filtering, then eluting the
antibacterial peptide adsorbed on the
alginic acid with the
hydrochloric acid solution, adjusting the pH value of the eluent to be 3.5, carrying out
precipitation with NaCl, carrying out
dialysis and desalting, drying and obtaining a crude
antibacterial peptide product. The method has the advantages of reasonable technical
route, low cost, high yield, large economic value, environmentally-friendly effect and effective utilization of the fly larvae resources for preparing
natural product varieties.