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Acid soluble fish skin collagen and its preparing method

A technology of fish skin collagen and collagen, which is applied in the field of biological products, can solve the problems of unreported acid-soluble collagen, and achieve the effects of white color, cost saving, and small loss of collagen

Active Publication Date: 2006-03-22
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented inventor describes an improved way to make pure seafood animal skirt collagen (SFCS), specifically sharkskin collagen obtained through various methods such as cold water immersion, lyophilization, dialysis, drying techniques like vacuum dehydrator/freezing, and cryo-displacement refrigerant storage technique. These technical results include increased yields, reduced cost per unit volume due to less expensive raw materials needed, no harmful chemical substances added during processing, minimal impurities found within these sources, and enhanced usability when preparing certain types of medications containing them.

Problems solved by technology

This patents discuss how different methods exist for producing pure collagen without causing decomposition during chemical processes like acids. Existing techniques involve complicated steps involving multiple reagents and solvent treatments, leading to increased manufacturing expenses. There is thus a technical problem addressed by this patented technology: finding effective ways to efficiently produce highly concentrated acid-insolubilized collagens derived from seafood sources instead of traditional chemically produced ones.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 1Kg of frozen fish skin, thaw it, drain it, and cut it into smaller than 0.5cm 3 of pieces. Add 10L of 2% NaCl solution, soak at 4°C for 18h, then centrifuge at 3000rpm for 20min, and discard the turbid supernatant. Then put the fish skin into the mixture of 5 L of acetone and 5 L of ethanol that had been pre-cooled at -20°C. After 4 hours, the fish skin was taken out and dried in a fume hood. Then dissolve the fish skin in a mixture of 13.7L 0.1mol / L citric acid and 1.36L 0.5mol / L acetic acid (10:1 total 15L), soak at 4°C for 24h, centrifuge at 10000rpm for 40min at 4°C, and remove the precipitate Repeat this step to extract twice more, combine the supernatants, and store them at 4°C to obtain an acid-soluble collagen solution; directly add 1.75Kg of NaCl solid to the above solution to make the final concentration reach 1mol / L, carry out salting out for 4 hours, and then Centrifuge at 4°C, 6000rpm for 20min, redissolve the collagen precipitate in 0.5mol / L acetic...

Embodiment 2

[0033] Take 1Kg of frozen fish skin, thaw it, drain it, and cut it into smaller than 0.5cm 3of pieces. Add 10L of 4% NaCl solution, soak at 4°C for 20h, then centrifuge at 3000rpm for 20min, and discard the turbid supernatant. Then put the fish skin into a mixture of 7.5 L of acetone and 7.5 L of ethanol that had been pre-cooled at -20°C. After 8 hours of exposure, the fish skin was taken out and dried in a fume hood. Then dissolve the fish skin in a mixture of 17.5L 0.1mol / L citric acid and 2.5L 0.5mol / L acetic acid (7:1 total 20L), soak at 4°C for 22h, centrifuge at 12000rpm for 20min at 4°C, and remove the precipitate Repeat this step to extract once more, combine the supernatant, and store at 4°C to obtain an acid-soluble collagen solution; directly add 4.68Kg of NaCl solid to the above solution to make the final concentration reach 2mol / L, carry out salting out for 12h, and then Centrifuge at 4°C and 8000rpm for 15 minutes, redissolve the collagen precipitate in 0.5mol / ...

Embodiment 3

[0035] Take 1Kg of frozen fish skin, thaw it, drain it, and cut it into smaller than 0.5cm 3 of pieces. Add 20L of 5% NaCl solution, soak at 4°C for 12h, centrifuge at 4000rpm for 10min at 4°C, and discard the turbid supernatant. Then put the fish skin into the mixture of 12.5 L of acetone and 12.5 L of ethanol that had been pre-cooled at -20°C. After 12 hours of action, the fish skin was taken out and dried in a fume hood. Then dissolve the fish skin in a mixture of 5L 0.1mol / L citric acid and 5L 0.5mol / L acetic acid (1:1, 10L in total), soak at 4°C for 20h, centrifuge at 10,000rpm for 50min at 4°C, and obtain the supernatant , stored at 4°C to obtain an acid-soluble collagen solution; directly add 3.5Kg of NaCl solid to the above solution to make the final concentration reach 3mol / L, carry out salting out for 1h, and then freeze and centrifuge at 6000rpm at 4°C for 15min to reconstitute the collagen precipitate. Dissolve in 0.5mol / L acetic acid solution, then in 0.02mol / L ...

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Abstract

The acid soluble fish skin collagen is prepared with sea fish skin as material and through the steps of cutting, NaCl solution soaking to eliminate impurity, alcohol and acetone defatting, soaking in mixed citric acid-acetic acid solution to extract, centrifugally separating to obtain acid soluble fish skin collagen solution, solid NaCl salt separation to obtain acid soluble fish skin collagen precipitate, dissolving in acetic acid solution, dialysis in Na2HPO4 solution or ultrafiltering with hollow acetate fiber film of molecular weight greater than 60 KDa, and vacuum freeze drying to obtain the product. The product is white, without fishy smell and spongy, and has purity over 97 %. It contains rich glycin, proline and hydroxyproline, as well as physiologically active peptide in its hydrolysate, and the collagen has molecular weight greater than 60 KDa.

Description

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Claims

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Application Information

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Owner DALIAN POLYTECHNIC UNIVERSITY
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