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Preparation method of oral biofilm

A biofilm and oral cavity technology, applied in the field of oral biofilm preparation, can solve problems such as high inhomogeneity, achieve the effects of low antigenicity, easy clinical operation and use requirements, good plasticity and mechanical properties

Active Publication Date: 2015-09-23
TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the main chain structure of the polysaccharide is not complex, it exhibits a high degree of heterogeneity in terms of the degree of sulfation, the distribution of sulfate groups, and the distribution of the two differences into the chain of the isomeric uronic acid

Method used

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  • Preparation method of oral biofilm

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preparation example Construction

[0029] Such as figure 1 Shown, a kind of oral cavity biofilm preparation method described in the embodiment of the present invention, this preparation method comprises the following steps:

[0030] Step 1, adding type I collagen to 0.2%-0.6% acetic acid solution to prepare collagen acetic acid swelling solution;

[0031] Specifically, the type I collagen is type I collagen extracted from bovine Achilles tendon, and when added to a 0.2%-0.6% acetic acid solution, the mass fraction of the type I collagen is controlled between 0.3%-0.8%. In between, stir at a high speed of 16000r-20000r per minute at a temperature of 15°C-25°C to prepare a collagen acetic acid swelling solution.

[0032] Step 2, adding chondroitin sulfate to the configured collagen acetic acid swelling solution, and stirring to form a collagen-chondroitin sulfate slurry;

[0033] Specifically, when chondroitin sulfate is added to the configured collagen acetic acid swelling solution, the mass fraction of chondr...

Embodiment 1

[0044] 1. Slurry Preparation

[0045] 1.1 Raw material thawing:

[0046] The type I collagen extracted from the bovine Achilles tendon was taken out from the freezer and thawed.

[0047] 1.2 Preparation of acetic acid solution:

[0048] Take 2-6ml of acetic acid to a 1000ml Erlenmeyer flask, add sterile water to 1000ml, mix well

[0049] 1.3 Slurry preparation:

[0050] Weigh an appropriate amount of thawed type I collagen, add it to 973ml of the above-mentioned acetic acid solution, calculate the amount of type I collagen according to the water content of type I collagen, control the mass fraction of type I collagen to 0.3%, stir for 1-2 hours, A collagen acetic acid swelling solution is formed.

[0051] Weigh 0.10g of chondroitin sulfate and add it to 27ml of the above acetic acid solution, stir well, filter and sterilize with a 0.22μm filter membrane, and slowly add it to the well-stirred collagen acetic acid swelling solution with a peristaltic pump to form collagen-su...

Embodiment 2

[0063] The preparation method of oral biofilm of the present invention, its system comprises the following steps:

[0064] 1. Slurry Preparation

[0065] 1.1 Raw material thawing:

[0066] Type I collagen was removed from the freezer and thawed.

[0067] 1.2 Preparation of acetic acid solution:

[0068] Take 2-6ml of acetic acid to a 1000ml Erlenmeyer flask, add sterile water to 1000ml, mix well;

[0069] 1.3 Slurry preparation:

[0070] Weigh an appropriate amount of thawed type I collagen, add it to 865ml of the above-mentioned acetic acid solution, calculate the amount of type I collagen according to the water content of type I collagen, control the mass fraction of type I collagen to 0.8%, stir for 1-2 hours, A collagen acetic acid swelling solution is formed.

[0071] Weigh 0.5g of chondroitin sulfate and add it to 135ml of the above acetic acid solution, stir well, filter and sterilize with a 0.22μm filter membrane, and slowly add it to the well-stirred collagen ace...

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Abstract

The invention relates to a preparation method of an oral biofilm. The preparation method comprises the following steps: adding type I collagen protein into an acetic acid solution to prepare a collagen-acetic acid swelling solution; adding chondroitin sulfate and stirring to prepare a collagen-chondroitin sulfate serous fluid; carrying out vacuum freeze-drying; pressing to prepare a collagen composite film; spraying the collagen-chondroitin sulfate serous fluid between two layers of the collagen composite film, and carrying out vacuum freeze-drying; carrying out high temperature vacuum crosslinking; and sterilizing after crosslinking to obtain a collagen-chondroitin sulfate composite oral biofilm. The invention has the following beneficial effects: the oral biofilm prepared by the above method has good histocompatibility, suitable controllable degradability and absorbability, low antigenicity and good plasticity and mechanical property; the oral biofilm is used in oral operation; and the three-dimensional porous structure of the oral film provides a tissue engineering scaffold for repairing of wounded tissues, is beneficial to invasion of cells and differential growth and can induce reparative regeneration of defective tissues, and specific thickness of the biofilm can effectively prevent ingrowth of soft tissue.

Description

technical field [0001] The invention relates to the field of oral medical materials, in particular to a method for preparing oral biofilm. Background technique [0002] Collagen is a biological polymer substance, the English scientific name Collagen. Plays the role of binding tissue in animal cells. It is one of the most critical raw materials in the biotechnology industry, and it is also the best biomedical material with a huge demand. Its application areas include biomedical materials, cosmetics, food industry, research purposes, etc. [0003] Collagen is a structural protein of the extracellular matrix in which its molecules aggregate into supramolecular structures. The molecular weight is 300KD. The most common structural feature of collagen is the triple helix structure. It is composed of three a-chain polypeptides, and each collagen chain is in a left-handed helical configuration. The three left-handed helical chain forks are intertwined to form a right-handed he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/56A61L27/58
Inventor 康承桂董会刘永霞
Owner TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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