183 results about "Triple helix" patented technology

Various aspects and embodiments of the present disclosure relate to methods and compositions that combine multiple mammalian RNA regulatory strategies, including RNA triple helix structures, introns, microRNAs, and ribozymes with Cas-based CRISPR transcription factors and ribonuclease-based RNA processing in human cells. The methods and compositions of the present disclosure, in some embodiments, enable multiplexed production of proteins and multiple guide RNAs from a single compact RNA-polymerase-II-expressed transcript for efficient modulation of synthetic constructs and endogenous human promoters.

A food product comprising one or more plastic food substrates is formed into a helical configuration and coated with a fluid barrier agent to prevent reannealing of adjacent turns of the product. The fluid barrier agent may be an edible vegetable oil such, for example, as hydrogenated vegetable oil, Soya oil, rape oil, sunflower oil, safflower oil, peanut oil or a mixture of such oils. The product may define a single helix or a multiple helix, e.g. a double or triple helix.

A power cable having improved durability and associated methods of assembly and use are described herein. In one aspect, the power cable is adapted for use in powering an implantable circulatory pump system. The cable includes one or more conductors of uninsulated wire strands that are loosely packed so as to move relative one another during cable flexure. The driveline cable may include a plurality of conductors, each comprised of multiple uninsulated bundles of uninsulated, loosely packed wire strands of a conductive material, that are wrapped about a central core. The cable may include at least six conductors, each conductor having at least 200 wire strands of a 30 gauge or higher. The cable may include the plurality of wire strands wound in a Litz style configuration to provide improved durability over many cycles of use at reduced cost, improved integrity of the electrical connection and reduced diameter.

High affinity, chemically modified triplex-forming oligonucleotides (TFOs) and methods for use thereof are disclosed. TFOs are defined as triplex-forming oligonucleotides which bind as third strands to duplex DNA in a sequence specific manner. Triplex-forming oligonucleotides may be comprised of any possible combination of nucleotides and modified nucleotides. Modified nucleotides may contain chemical modifications of the heterocyclic base, sugar moiety or phosphate moiety. A high affinity oligonucleotide (Kd≦2×10−8) which forms a triple strand with a specific DNA segment of a target gene DNA is generated. It is preferable that the Kd for the high affinity oligonucleotide is below 2×10−10. The nucleotide binds or hybridizes to a target sequence within a target gene or target region of a chromosome, forming a triplex region. The binding of the oligonucleotide to the target region stimulates mutations within or adjacent to the target region using cellular DNA synthesis, recombination, and repair mechanisms. The mutation generated activates, inactivates, or alters the activity and function of the target gene.

A novel collagen mimic comprising a tripeptide unit having the formula (flpYaaGly)n, where flp is 4(S)-fluoro-L-proline, is disclosed. The collagen mimic has increased stability relative to the collagen-related triple helices (ProYaaGly)n, (hypYaaGly)n, and (HypYaaGly)n.

Method for double-stranded DNA purification, by which a solution containing said DNA in a mixture with other components is passed over a support on which is covalently coupled in oligonucleotide capable of hybridizing with a specific sequence present on said DNA to form a triple helix.

The invention discloses a preparation method of a pepsin-soluble high-purity superhelical-structured type-I collagen, i.e. a low-antigenicity high-purity superhelical-structured type-I collagen. The preparation method is characterized by comprising the following steps: carrying out alkali treatment on scales which mainly contain the type-I collagen and serves as a raw material to remove hybrid proteins and fats, then carrying out acid treatment to remove calcium salts of the scales, adding a weak acid solution as an extraction agent, simultaneously adding pepsin, carrying out low-temperature stirring and leaching, and after the leaching is completed, carrying out refrigerated centrifugation so as to obtain crude leach liquor of the pepsin-soluble type-I collagen; purifying the crude leach liquor of the pepsin-soluble type-I collagen by using a membrane separation technology, and freeze-drying, so that the pepsin-soluble type-I collagen with a purity of not less than 90% and kept at a triple helix structure is obtained. A collagen product prepared by using the method disclosed by the invention is low in antigenicity, clear in configuration, and integral in triple helix structure; HPLC detection shows that the product is a type-I collagen with a purity of greater than or equal to 90%, and MALDI-TOF-MS detection shows that a mass spectrogram of the product has no impure peak, and the molecular weight is 288 kDa.

The invention discloses soluble non-denatured II type collagen and a preparation method thereof. The method comprises the following steps: unfreezing cartilage, draining, weighing, homogenizing, sterilizing, disinfecting, removing impurities, carrying out enzymolysis, salting out, washing, drying and crushing to obtain soluble non-denatured II-type collagen powder. The soluble non-denatured II-type collagen has the advantages that the solubility is good, and the active part of the soluble non-denatured II-type collagen can be fully released in the intestinal tract; the soluble non-denatured II-type collagen is high in purity, a triple helix structure is kept complete, and the activity of improving arthritis is high; an extraction method of the soluble non-denatured II-type collagen is highin extraction rate, short in extraction time, high in safety, free of harmful substance residues and good in stability, so that the extraction of the soluble non-denatured II-type collagen is expanded to the production scale, and the production requirements are basically met. The technical scheme is simple and easy to implement, high in yield, short in time, low in cost and high in product activity.

A modular strake for reducing the effects of wind on utility structures is disclosed. The strake is comprised of individual fin sections that may easily be attached to a utility structure, preferably in a triple helix pattern. The modular strakes may be installed on a utility structure after is has been placed into service.