183 results about "Triple helix" patented technology

Various aspects and embodiments of the present disclosure relate to methods and compositions that combine multiple mammalian RNA regulatory strategies, including RNA triple helix structures, introns, microRNAs, and ribozymes with Cas-based CRISPR transcription factors and ribonuclease-based RNA processing in human cells. The methods and compositions of the present disclosure, in some embodiments, enable multiplexed production of proteins and multiple guide RNAs from a single compact RNA-polymerase-II-expressed transcript for efficient modulation of synthetic constructs and endogenous human promoters.

The invention relates to the gene encoding the mammalian prostaglandin H synthase-2 and its product. More specifically, the invention relates to the diagnosis of aberrant PGHS-2 gene or gene product; the identification, production, and use of compounds which modulate PGHS-2 gene expression or the activity of the PGHS-2 gene product including but not limited to nucleic acid encoding PGHS-12 and homologues, analogues, and deletions thereof, as well as antisense, ribozyme, triple helix, antibody, and polypeptide molecules as well as small inorganic molecules; and pharmaceutical formulations and routes of administration for such compounds.

The invention relates to an I-type medical collagen material keeping an original specific triple-helical structure of collagen, and an extraction method thereof, meninges / spinal meninges biomembrane made from the I-type medical collagen material, and a preparation process of the biomembrane, and application of the biomembrane to preparing meninges / spinal meninges tissue repair materials. The I-type medical collagen material keeping the original specific triple helix structure of collagen has the advantages of low immunogenicity, no foreign body reaction, good biocompatibility and controllabledegradation rate, the prepared meninges / spinal meninges biomembrane has certain stretching resistance strength, repairable and regenerative dura mater / spinal meninges tissue and tissue adhesion prevention / reduction, and the biomembrane is applicable to repair and regeneration of injured cerebral dura mater and spinal dura mater.

A power cable having improved durability and associated methods of assembly and use are described herein. In one aspect, the power cable is adapted for use in powering an implantable circulatory pump system. The cable includes one or more conductors of uninsulated wire strands that are loosely packed so as to move relative one another during cable flexure. The driveline cable may include a plurality of conductors, each comprised of multiple uninsulated bundles of uninsulated, loosely packed wire strands of a conductive material, that are wrapped about a central core. The cable may include at least six conductors, each conductor having at least 200 wire strands of a 30 gauge or higher. The cable may include the plurality of wire strands wound in a Litz style configuration to provide improved durability over many cycles of use at reduced cost, improved integrity of the electrical connection and reduced diameter.

High affinity, chemically modified triplex-forming oligonucleotides (TFOs) and methods for use thereof are disclosed. TFOs are defined as triplex-forming oligonucleotides which bind as third strands to duplex DNA in a sequence specific manner. Triplex-forming oligonucleotides may be comprised of any possible combination of nucleotides and modified nucleotides. Modified nucleotides may contain chemical modifications of the heterocyclic base, sugar moiety or phosphate moiety. A high affinity oligonucleotide (Kd≦2×10−8) which forms a triple strand with a specific DNA segment of a target gene DNA is generated. It is preferable that the Kd for the high affinity oligonucleotide is below 2×10−10. The nucleotide binds or hybridizes to a target sequence within a target gene or target region of a chromosome, forming a triplex region. The binding of the oligonucleotide to the target region stimulates mutations within or adjacent to the target region using cellular DNA synthesis, recombination, and repair mechanisms. The mutation generated activates, inactivates, or alters the activity and function of the target gene.

The present invention relates to a collagen-related polypeptide (CRP) having hydrophobic amino acid groups at the N- and C-termini capable of non-covalent self-assembly into collagen mimetic triple helices and fibrils thereof and the synthesis, methods of use and compositions thereof.

The invention discloses a method for extracting collagen from crucian carp skin. The method comprises steps of preprocessing, washing, enzymatic extraction, salting out, dialysis and freeze drying. The method has the following beneficial effects: the method adopts simple preparation steps andrequires a small quantity of devices, accurate data is provided for each step of operation, the reproducibility is good, and the method is suitable for large-scale production; the collagen is prepared from the crucian carp skin, namely, the crucian carp processing leftover as a raw material, the cost is low, the rate of utilization of the crucian carp is increased, and added value of the crucian carp is increased; the prepared collagen is high in biological activity and can be applied to the fields of drugs, food, health care products, cosmetics and the like; the speed for extracting the collagen with an enzymatic hydrolysis method is high, non-helical telopeptide can be selectively removed by pepsin, so that collagen molecules can be dissolved out, the triple helix structure of the collagen is retained, and the biological activity of the collagen is maintained.

The invention provides a method for preparing natural ossein, which adopts extraction and purification from beasts and birds bones and comprises the following steps: cleaning; crushing; degreasing and inorganic substance removal; acid soaking; enzyme hydrolysis; separation and purification; and freeze drying. The natural ossein prepared by the method can keep a complete collagen triple helix structure so that the biological activity is retained, the purity can reach more than 90 percent, and the natural ossein has higher extraction yield and can be widely applied in the fields of foods, cosmetics, biomedicine and the like.

A high affinity, triplex-forming oligonucleotide and methods for use thereof wherein an oligonucleotide is used to form a triple-stranded nucleic acid molecule with a specific DNA segment of a target DNA molecule. Upon formation of the triplex, the binding of the oligonucleotide stimulates mutagenesis within or adjacent to the target sequence using cellular DNA synthesis or repair mechanisms thereby producing heritable changes in a human or animal. The mutation activates, inactivates or alters the activity and function of the target molecule. This mutation may be the result of a recombinagenic mechanism induced by the oligonucleotide.

The invention relates to a preparation method of collagen; fresh pigskin is used as a raw material; the raw material is treated by pretreatment, fat removal, and acid-enzyme treatment; ultrasonic treatment is employed to further improve extraction efficiency; and finally purification is performed to prepare a collagen product with high purity. The preparation method of collagen has high extraction efficiency, and the product purity is up to above 98%. The product is active collagen with a maintained triple-helix structure, has low immunogenicity and high biocompatibility, is biodegradable, and is applicable to medical purposes.

The present invention relates to biocompatible and biodegradable stimuli-sensitive polymeric nanoparticles, which were formed by ion-ion interaction in aqueous media. Synthetic and biological macromolecules with ionizable functional groups are capable of forming nanoparticles whose size and surface properties are sensitive to environmental influences such as pH, temperature and salt concentration. Nanodevices are designed for therapeutic applications as drug and nucleic acid carriers, and / or for MRI diagnosis as contrast agents. These nanodevices are designed for therapeutic applications as targeted drug carriers. Additionally, they can be used as contrast agents for MRI diagnosis. These nanosystems are also potential carriers for delivery of active ingredients as DNA, RNA, short interfering RNA (siRNA), antisense oligonucleotides (AS-ON), and triple helix forming oligonucleotides (TFO) etc. for pharmaceutical applications. Their adjustable size offers yet another advantage.

The invention relates to the field of biomedical materials, and particularly provides a fish collagen repair sponge and preparation method thereof. The preparation method comprises the following steps: using fish skin as a raw material; degreasing, enzymatically hydrolyzing, purifying and filtering to obtain a fish collagen raw material; using the fish collagen raw material as the raw material for re-dissolution and self-assembly treatment; and then, freeze-drying and cross-linking to obtain the fish collagen repair sponge. The invention provides the fish collagen repair sponge which is simple in preparation process and low in price and overcomes some shortcomings of the existing collagen sponge. The fish collagen repair sponge utilizes a unique triple-helix structure of the fish collagen, has an excellent microstructure through self-assembly to a certain degree, has the advantages of high porosity, controllable mechanical properties and good biodegradability, and is an excellent biomedical material.

The invention relates to matrix metallic protease-directed recombination human tumor necrosis factor fused protein and a method for making the same. The amino acid sequence of the fused protein consists of three parts, i.e. a collagen sequence, a (a sort or a class of) human matrix metallic protease substrate sequence and a human tumor necrosis factor-alpha sequence (as shown in graph 1) which are arranged in turn from an N-end. When tumor necrosis factor-alpha is formed into a trimerical active form, the collagen sequence is formed into right hand triple helix, and plays a role in closing the recipient binding site of tumor necrosis factor-alpha along with the human matrix metallic protease substrate sequence. The specific cutting of the fused protein can be completed by matrix metallic protease excreted by a tumor tissue inside the tumor tissue so as to expose the recipient binding site of tumor necrosis factor-alpha and to represent cytotoxic activity; however, the fused protein does not represent cytotoxic activity in most normal tissues. The fused protein has tumor-targeting characteristics and the action of killing tumor cells, thereby having enormous application prospect in curing tumor.

The present invention provides a food product comprising one or more plastic food substrates formed into a helical configuration and coated with a fluid barrier agent to prevent reannealing of adjacent turns of the product. The fluid barrier agent may be an edible vegetable oil such, for example, as hydrogenated vegetable oil, Soya oil, rape oil, sunflower oil, safflower oil, peanut oil or a mixture of such oils. The product may define a single helix or a multiple helix, e.g. a double or triple helix. The invention also provides a method and apparatus for making such a helical food product.

The present invention provides recombinant triple helical proteins or collagen-like proteins comprising a prokaryotic protein or one or more domains of a prokaryotic protein comprising a collagen-like peptide sequence of repeated Gly-Xaa-Yaa triplets and, optionally, one or more domains from a mammalian collagen. Also provided are expression vectors and host cells containing the expression vectors to produce these recombinant proteins and methods of production for the same. Additionally, antibodies are provided that are directed against a recombinant collagen-like protein that, preferably, binds an integrin. Furthermore, a method of screening for potential therapeutic compounds that inhibit the integrin-binding or -interacting activities of recombinant collagen-like proteins.

The invention relates to an extraction method for triple helical edible fungus amylase, the specific steps of which are that firstly, crashed natural bamboo fungus is degreased with an organic solvent in a soxhlet extractor, secondarily, the residue is sequentially extracted with water in the gradient temperature of 20-80 DEG C, filtered to obtain filtrate, and repeated for three times; the supernatant fluid is taken by decentralization to obtain an extraction solution; the proteic extraction is carried out on the extraction solution by a Sevage method, and then the extraction solution is respectively dialyzed with clear water and secondary distilled water after being decolored by H2O2, and after the DEAE pillar removes the acidic polysaccharide, the solution is frozen and dried to obtain the white cotton-shaped triple helical edible fungus amylase. The extraction method is easy to be implemented; and the product has high purity, is discovered for the first time and verifies the triple helical structure of the edible fungus amylase, and has wide application prospect in the aspects of medicine and material.

Method for double-stranded DNA purification, by which a solution containing said DNA in a mixture with other components is passed over a support on which is covalently coupled in oligonucleotide capable of hybridizing with a specific sequence present on said DNA to form a triple helix.

The present invention relates to a collagen-related polypeptide (CRP) having hydrophobic amino acid groups at the N- and C-termini capable of non-covalent self-assembly into collagen mimetic triple helices and fibrils thereof and the synthesis, methods of use and compositions thereof.

The product according to the present invention is produced in a production process using the mycelium of a mushroom such as Ganoderma lucidum. The production process encourages the growing mycelium to export bioactive compounds such as beta glucans into the surrounding liquid. Thus, the beta glucan is soluble and easily absorbed. More importantly, the production process allows the beta glucan to retain its triple helix structure which is required for maintain a very high bioactive efficacy. The beta glucans from Ganoderma lucidum can be used in a cream for treatment of psoriasis.

The invention provides a Ti plasmid aspergillus niger gene replacement expression vector and application thereof, and belongs to the technical field of molecular biology. The T-DNA (Triple helix Deoxyribose Nucleic Acid) region elements of the Ti plasmid aspergillus niger gene replacement expression vector are arranged in the following sequence: an aspergillus niger target gene promoter, a multiple cloning site, an aspergillus niger target gene terminator, an aspergillus nidulans 3-phosphoglyceraldehyde dehydrogenase gene promoter PgpdA, an aspergillus niger selection marker gene and an aspergillus niger target gene terminator. According to the invention, a target gene is integrated at the site of the aspergillus niger target gene through homologous recombination, and the target gene is regulated and controlled by a target gene promotor of high expression; therefore, the position effect of transgenosis is eliminated and the expression level is improved.

The invention discloses a preparation method of a pepsin-soluble high-purity superhelical-structured type-I collagen, i.e. a low-antigenicity high-purity superhelical-structured type-I collagen. The preparation method is characterized by comprising the following steps: carrying out alkali treatment on scales which mainly contain the type-I collagen and serves as a raw material to remove hybrid proteins and fats, then carrying out acid treatment to remove calcium salts of the scales, adding a weak acid solution as an extraction agent, simultaneously adding pepsin, carrying out low-temperature stirring and leaching, and after the leaching is completed, carrying out refrigerated centrifugation so as to obtain crude leach liquor of the pepsin-soluble type-I collagen; purifying the crude leach liquor of the pepsin-soluble type-I collagen by using a membrane separation technology, and freeze-drying, so that the pepsin-soluble type-I collagen with a purity of not less than 90% and kept at a triple helix structure is obtained. A collagen product prepared by using the method disclosed by the invention is low in antigenicity, clear in configuration, and integral in triple helix structure; HPLC detection shows that the product is a type-I collagen with a purity of greater than or equal to 90%, and MALDI-TOF-MS detection shows that a mass spectrogram of the product has no impure peak, and the molecular weight is 288 kDa.

The invention discloses a method for extracting unmodified natural collagen from bullfrog skin. The method is characterized by comprising the following steps of: treating washed bullfrog skin by using NaOH solution, H2O2 solution and a degreasing agent in the treatment phase to remove non-collagen protein, pigments and fat; leaching acetic acid solution and acetic acid-protease in the extraction phase to obtain acid-soluble collagen and enzyme-soluble collagen; performing salting-out for many times by utilizing a salt in the purification phase; and dialyzing and performing freeze-drying to obtain spongy bullfrog skin collagen. A lauryl sodium sulfate-polyacrylamide gel electrophoretogram indicates that the bullfrog skin collagen contains a gamma chain with the molecular weight of 0.3 million, a beta chain with the molecular weight of 0.2 million and alpha chains with the molecular weight of 0.1 million, wherein the alpha chains are two, and a triple helix structure of the collagen is better reserved. Unless otherwise specified, the operation temperature is below 15 DEG C in the extraction process, the dry weight extraction rate of the obtained product is more than or equal to 80 percent, and the collagen is white in color and high in quality.

The invention belongs to the field of leather tanning technologies, and relates to a less-chrome tanning technology for tanning sheep skin through a nano-composite high absorption chrome tanning assistant. According to the traditional tanning technology, the absorption rate of chrome is not high, so serious waste of chrome resource and pollution are caused and the ecological environment is damaged. According to the tanning theory and the structure characteristics of collagenous fibers and nano-composite high absorption chrome tanning assistant, the nano-composite high absorption chrome tanning assistant and a little of chrome powder are matched and used for tanning goat acid skin, wet blue shrinkage temperature exceeds 100 DEG C, the thickening rate is higher by 50% than that of the leather tanned with conventional 8% of chrome powder, the content of Cr2O3 in the waste liquid is reduced to 50mg / L, and the absorption rate of chrome is improved to be 99%. The mechanical strength of the crust leather is equivalent to normally chrome tanned leather, and SEM (scanning electron microscope) representation results show that the nano-composite high absorption chrome tanning assistant is beneficial to scattering the collagenous fibers, but does not damage the triple helix structure of the collagenous fiber, so that the stability of the collagen structure is guaranteed.

The invention provides a recombinant humanized III-type collagen. The recombinant humanized III-type collagen has a triple helix structure, and has an amino acid sequence reducing a humanized III-typecollagen alpha-1 chain and including 1068 amino acids. The invention further provides a preparation method of the recombinant humanized III-type collagen, wherein the prolyl hydroxylase of PBCV-1 includes 209 amino acids, and the base sequence is optimized according to the codons of saccharomyces cerevisiae. Compared with the prior art, the preparation method has the advantages that the triple helix is formed in vitro from collagen chains by utilizing the prolyl hydroxylase; and the saccharomyces cerevisiae expression system adopted by the invention is applicable to large-scale amplificationwithout endotoxin or later removal, the preparation cost is low, and the protein expression quantity is further improved. The recombinant humanized III-type collagen is excellent in stability, the amino acid composition of the recombinant humanized III-type collagen is almost completely the same as that of the alpha-1 chain of a natural collagen, and no immunological rejection can be generated when the recombinant humanized III-type collagen is applied to a human body. The recombinant humanized III-type collagen is good in biocompatibility and can be widely applied to surgical treatment and cosmetic medical industry.

The invention discloses soluble non-denatured II type collagen and a preparation method thereof. The method comprises the following steps: unfreezing cartilage, draining, weighing, homogenizing, sterilizing, disinfecting, removing impurities, carrying out enzymolysis, salting out, washing, drying and crushing to obtain soluble non-denatured II-type collagen powder. The soluble non-denatured II-type collagen has the advantages that the solubility is good, and the active part of the soluble non-denatured II-type collagen can be fully released in the intestinal tract; the soluble non-denatured II-type collagen is high in purity, a triple helix structure is kept complete, and the activity of improving arthritis is high; an extraction method of the soluble non-denatured II-type collagen is highin extraction rate, short in extraction time, high in safety, free of harmful substance residues and good in stability, so that the extraction of the soluble non-denatured II-type collagen is expanded to the production scale, and the production requirements are basically met. The technical scheme is simple and easy to implement, high in yield, short in time, low in cost and high in product activity.

The invention discloses a nucleic acid detecting method based on a cascade photoelectric active material and a triple helix molecular switch. A semiconductor ZnO nano material and CdTe quantum dots with different particle diameters are modified on the surface of an electrode to form a cascade structure, which greatly enhances the photocurrent intensity. Through the formation and disassembly of triple helix molecules, the superiority of the triple helix molecular switch opens up new strategies for the high specificity detection of nucleic acids, and ultrasensitive detection of nucleic acids isrealized by means of an electrochemical workstation. According to the invention, a photoelectrochemical sensor can also be used for the detection of various nucleic acids by changing the sequence of the adapted DNA; and the method has the advantages of low cost, high sensitivity and rapid response, and has an important application prospect in biological analysis and clinical diagnosis.

A modular strake for reducing the effects of wind on utility structures is disclosed. The strake is comprised of individual fin sections that may easily be attached to a utility structure, preferably in a triple helix pattern. The modular strakes may be installed on a utility structure after is has been placed into service.