The invention relates to a preparation for a tussore silk
fibroin solution and a concentration method thereof.
Fibroin fiber of tussores like tussah, japanese tusser, costor silkworm, and Actias selene Hubner are decontaminated, degummed and then dipped into a dissolving liquid containing neutral salt and
alcohol materials, treated by ultrasonic for 5 to 120 minutes, and then dissolved under the temperature between 30 and 98 DEG C to obtain a silk dissolving
fibroin. The
fibroin dissolving liquid is dialyzed and desalted to obtain a tussore fibroin aqueous liquid; then the aqueous liquid is dewatered and concentrated by a
semipermeable membrane by using a high water-absorbing matter to obtain the
high concentration tussore fibroin solution with a molecular weight of 15 to 250 kDa and a concentration of 6 to 35 percent. As the neutral salt is used, the large extent hydrolyzation of acid and alkali to the silk fibroin is avoided; therefore, the molecular weight of the obtained tussore silk
protein is higher; the tissore silk fibroin can be used for manufacturing tussore silk fibroin gel,
powder, films, porous sponges, regenerated fibers, and the like. The invention provides new material for the fields of
biomedicine,
medicine, make-up, food, health product, and the like.