87 results about "Tissue equivalent" patented technology

A nanodosimeter device (15) for detecting positive ions induced in a sensitive gas volume by a radiation field of primary particle, comprising an ionization chamber (10) for holding the sensitive gas volume to be irradiated by the radiation field of primary particles; an ion counter system connected to the ionization chamber (10) for detecting the positive ions which pass through the aperture opening and arrive at the ion counter (12) at an arrival time; a particle tracking system for position-sensitive detection of the primary particles passing through the sensitive gas volume; and a data acquisition system capable of coordinating the readout of all data signals and of performing data analysis correlating the arrival time of the positive ions detected by the ion counter system relative to the position sensitive data of primary particles detected by the particle tracking system. The invention further includes the use of the nanodosimeter for method of calibrating radiation exposure with damage to a nucleic acid within a sample. A volume of tissue-equivalent gas is radiated with a radiation field to induce positive ions. The resulting positive ions are measured and compared with a determination of presence or extent of damage resulting from irradiating a nucleic acid sample with an equivalent dose of radiation.

The present invention provides new methods for the in vitro preparation of bioartificial tissue equivalents and their enhanced integration after implantation in vivo. These methods include submitting a tissue construct to a biomimetic electrical stimulation during cultivation in vitro to improve its structural and functional properties, and / or in vivo, after implantation of the construct, to enhance its integration with host tissue and increase cell survival and functionality. The inventive methods are particularly useful for the production of bioartificial equivalents and / or the repair and replacement of native tissues that contain electrically excitable cells and are subject to electrical stimulation in vivo, such as, for example, cardiac muscle tissue, striated skeletal muscle tissue, smooth muscle tissue, bone, vasculature, and nerve tissue.

A deformable phantom apparatus for simulating motion of a patient's anatomy in 3D during breathing, the apparatus comprising a chamber fillable with a first fluid, a deformable member comprising tissue equivalent material of the anatomy being simulated, the deformable member being positionable within the chamber in the first fluid and having an open end in fluid communication with a second fluid outside the chamber in use; and a mechanism for causing the second fluid to flow through the open end to deform the deformable member between a normal state and a deformed state to simulate motion during breathing.

Disclosed is a phantom for dose verification for intensity-modulated radiation therapy having a base of substantially tissue-equivalent material and a two-dimensional array of cavities formed in the base with each the cavities being configured and dimensioned to receive a radiation detector.

The method of growing a fungal fruiting body requires exposing a mycelium of a desired organism type to environmental conditions sufficient to induce fruiting of fungal primordium in the organism type followed by enclosing the fungal primordium within a mold of a designated shape representing a near net shape volume of a desired final product. The fungal primordium is allowed to grow and fill the mold to form a mass of fungal tissue equivalent in shape to the designated shape of the mold after which the mass of fungal tissue is removed from the mold and dried.

Disclosed is a cervico-vaginal tissue equivalent comprised of vaginal epithelial cells and immune cells, cultured at the air-liquid interface. The tissue equivalent is capable of being infected with a sexually transmitted pathogen such as a virus (e.g., HIV), a bacteria, a helminthic parasite, or a fungus. The tissue equivalent is also capable of undergoing an allergic-type reaction or an irritant-type reaction. The tissue equivalent is characterized as having nucleated basal layer cells and nucleated suprabasal layer cells, and further as having cell layers external to the suprabasal layer progressively increasing in glycogen content and progressively decreasing in nuclei content. Immune cells of the tissue equivalent are primarily located in the basal and suprabasal layers. Also disclosed are methods for producing the tissue equivalent. The methods involve providing vaginal epithelial cells and immune cells, seeding the cells onto a porous support, and co culturing the seeded cells at the air-liquid interface under conditions appropriate for differentiation. One such method disclosed is for generation of the tissue equivalent in serum free medium. Specific cells from which the tissue equivalent is generated, and also specific preferred components of the medium in which the tissue equivalent is generated are provided. Also disclosed is a cervico-vaginal tissue equivalent produced by the methods disclosed herein.

The present invention is directed to an anti-adhesion patch, which is constructed using a tissue equivalent technique. The anti-adhesion patch comprises a collagenous material and at least one non-living cellular component. Also provided is a method for preventing tissue adhesions between organs and other tissues being operated upon during surgical procedures by utilizing the anti-adhesion patch disclosed herein.

A microdosimeter, comprising an array of three-dimensional p-n junction semiconductor detectors, each providing a sensitive volume-target and a tissue equivalent medium for generating secondary charged particles. The array is manufactured from a semiconductor on insulator wafer and the detectors are located to detect secondary charged particles generated in the tissue equivalent medium.

The present invention provides a three-dimensional tissue equivalent for in-vivo and in-vitro uses. The three dimensional tissue equivalent of the present invention is a non-contractile cellular sheet cultured over a porous scaffold using a macromass culturing technique, for example where the cellular sheet is entirely on one side of a porous sponge. In one embodiment, the present invention provides a dermal wound dressing that comprises a high density cellular sheet of dermal fibroblast cells.

Calibration and reference samples with reduced cross-sectional areas encased within imaging tables or couch pads have low attenuation properties and provide patient comfort. The samples are stable and provide reproducible images without artifacts. The torso-length samples avoid positioning errors and misalignment. Sample density or mass calibration materials include calcium compounds representative of bone and calcifications, iodine compounds for contrast angiography, gadolinium compounds for MRI, and fat and tissue equivalent materials. Density corrections for variable patient scatter and imperfect image reconstructions improve quantitative measurement. Automated computer methods detect the samples and record readings on all images over the extent of the scans without operator interaction. Spatial references function as location references and enable spatial correction of device imperfections such as point spread function (PSF) or motion for improved images. Comparative analysis of backward and forward projections corrects images based on simultaneous imaging of the references of known properties.

The present invention is a thorax phantom that enables simulation of tumor motion within a tissue equivalent material. The system consists of a tissue equivalent epoxy phantom representing a 15 cm axial section of the human thorax that includes simplified spine and lung anatomies. Within the phantom are thru rods of similar tissue density. The rods are attached to a computer-controlled actuator that facilitates both linear and rotational motion of the rods within the phantom. A plurality of tumor targets and radiation detectors can be placed within the rods at various locations thereby enabling the simulation of respiratory and cardiac induced tumor motions within the phantom and assessment of the effects of these motions on image acquisitions, treatment planning and radiation treatment delivery.

The method of growing a fungal fruiting body requires exposing a mycelium of a desired organism type to environmental conditions sufficient to induce fruiting of fungal primordium in the organism type followed by enclosing the fungal primordium within a mold of a designated shape representing a near net shape volume of a desired final product. The fungal primordium is allowed to grow and fill the mold to form a mass of fungal tissue equivalent in shape to the designated shape of the mold after which the mass of fungal tissue is removed from the mold and dried.

Normal tissue complications limit curative broadbeam radiotherapy to tumors including lung cancer. Radiation retinitis causing blindness limits quality of life and long term survival for patients with ocular melanoma. This invention pertains to alternative, normal tissue sparing 100 to 1,000 Gy microbeam radiations with least normal tissue complications and concomitant radio-immunotherapy by innate immune response of epidermis and dermis to low dose radiation with 50 kV X-rays. Total body skin radiation with former airport passenger screening machines with 50 kV X-ray is disclosed. Microbeams are generated without contaminating scatter and neutron radiations from collinear gamma ray and electron beam produced by inverse Compton interaction with high energy laser and electron beam and from proton and carbon ion beams in tissue equivalent cylindrical collimators. Extracorporeal immunotherapy and chemotherapy and apheresis of mutated subcellular particles released into circulation in response to cancer-therapies are by clinical continuous flow ultracentrifugation combined chromatography.

The present invention comprises the use of follicular dendritic cells (FDCs) or FDC-like cells to generate FDC-dependent, but T cell-independent, B cell responses to T cell-dependent antigens, with antigen-specific and polyclonal antibody production in ˜48 h. In another embodiment, a germinal center (GC) lymphoid tissue equivalent (LTE) was used to generate antigen-specific IgM, followed by switching to IgG. The GC LTE model can be used in vaccine assessment. Dual forms of immunogen were used in the GC LTE and in vivo. Dual immunogens resulted in rapid, specific IgM responses and enhanced IgG responses. This vaccine design approach can be used, for example, to provide rapid IgM protection (˜24-48 h) and high-affinity IgG more quickly in people moving to areas with endemic disease, or in people with T cell insufficiencies, who can be immunized to rapidly generate protective IgM.

A microdosimeter, comprising an array of three-dimensional p-n junction semiconductor detectors, each providing a sensitive volume-target and a tissue equivalent medium for generating secondary charged particles. The array is manufactured from a semiconductor on insulator wafer and the detectors are located to detect secondary charged particles generated in the tissue equivalent medium.

The present invention is a thorax phantom that enables simulation of tumor motion within a tissue equivalent material. The system consists of a tissue equivalent epoxy phantom representing a 15 cm axial section of the human thorax that includes simplified spine and lung anatomies. Within the phantom are thru rods of similar tissue density. The rods are attached to a computer-controlled actuator that facilitates both linear and rotational motion of the rods within the phantom. A plurality of tumor targets and radiation detectors can be placed within the rods at various locations thereby enabling the simulation of respiratory and cardiac induced tumor motions within the phantom and assessment of the effects of these motions on image acquisitions, treatment planning and radiation treatment delivery.

A deformable phantom apparatus for simulating motion of a patient's anatomy in 3D during breathing, the apparatus comprising a chamber fillable with a first fluid, a deformable member comprising tissue equivalent material of the anatomy being simulated, the deformable member being positionable within the chamber in the first fluid and having an open end in fluid communication with a second fluid outside the chamber in use; and a mechanism for causing the second fluid to flow through the open end to deform the deformable member between a normal state and a deformed state to simulate motion during breathing.

This invention relates to adaptive resistance inhibiting 100 to 10,000 Gy, single fraction proton and carbon ion microbeam and nanobeam radiosurgery, proton spray chemotherapy and proton spray gadolinium and boron neutron capture therapy with least normal tissue toxicity. Secondary neutrons, protons, ions and radiation from the accelerator and the patient specific collimator are removed with tissue equivalent collimator that also generates proton and carbon ion microbeam and nanobeam. Laser interaction with micrometer and nanometer thick metallic and diamond like carbon targets are also used for proton and carbon ion beam generation. Polyenergetic proton beam is spatially separated. Ion beam and Laser ion beam is accelerated in a hybrid RF accelerator. It is split into microbeam and nanobeam. Secondary neutrons, protons and gamma radiation are removed with tissue equivalent collimators that also generate microbeams and nanobeams. Adaptive resistance to cancer treatment is inhibited by dual target radiation, DNA strands break and enzymes inactivation.

Apparatus, including a mockup cavity, simulating an actual body cavity of a human subject, wherein walls defining the mockup cavity comprise a tissue equivalent material (TEM). An array of electrodes are embedded in the walls. The apparatus further includes a programmable signal generator, connected to the electrodes, and configured to apply varying potentials to the array of electrodes so as to simulate electrophysiological potentials occurring in the actual body cavity on surfaces of the walls.

An Active Water Phantom is designed to provide fast, accurate, high resolution, complete Quality Assurance of patient-specific treatment plans utilizing intensity-modulated Ion Beam Therapy, prior to their delivery to the patient. The detection medium is a tissue-equivalent water-based liquid scintillator material. A three-dimensional pattern of scintillation light, emitted upon ion beam irradiation, is reconstructed from three orthogonal two-dimensional light yield profiles, which are read out for each individual beam energy layer. The 3-D information has dose measurement accuracy 1-2% and spatial resolution 1-2 millimeters. The measurement sequence provides up to four orders of magnitude more data characterizing the treatment plan than currently commercially available alternatives, all in a time period no greater than that needed for actual delivery of the dose fraction to a patient. The system provides sophisticated control and readout of the cameras or photo-detectors, data archiving and analysis, simulation capabilities, and 3-D dose image reconstruction and visualization.

Quality assurance device for calibrating and testing the accuracy of movement-correlated computed tomography (“4D CT”) target-locating systems has a test unit sub-assembly adapted to be combined with a dynamic phantom system. The test unit sub-assembly has an axially and rotationally moveable test rod slideably disposed inside of a substantially hollow fixed housing. A matrix of markers, or “fiducials”, are located in the wall of the housing. A single fiducial is located near the distal end of the moveable test rod. The distal end portion of the moveable test rod is adapted to be connected to a motion actuator, which is programmed to oscillate the test rod in a predetermined pattern. When the test unit sub-assembly is inserted into a tissue equivalent phantom member, the combined sub-assembly and phantom member can then be subjected to four-dimensional imaging to generate a visual image. A visual comparison actual relative positions of the fiducials to the know positions of the fiducials in time indicates the accuracy of the 4D CT system.

The invention relates to a tissue equivalent replenisher for radiotherapy and a preparation method thereof. The preparation method comprises: a doctor determines a skin part needing tissue equivalent replenisher customization according to a range needing radiotherapy of a patient; the doctor uses a CT scanner to scan the skin part, obtains a CT image, and transmits the CT image to a work station; the work station constructs a three-dimensional contour model of the skin part based on the CT image, generates a data file, and sends the data file to a 3D printer; the doctor determines the tissue equivalent replenisher thickness based on a dosage requirement of the radiotherapy for the patient and inputs the thickness value into the 3D printer; and the 3D printer receives the data file and the e thickness value in of the tissue equivalent replenisher and selects materials for printing and preparation, thereby obtaining the tissue equivalent replenisher. According to the method, no gap is formed between the tissue equivalent replenisher and the skin of the human body based on individual customization; and thus the injury of the skin due to a secondary building effect caused by a high-energy ray in the gap during a radiotherapeutic process can be reduced.

A tissue equivalent for transplantation having a three-dimensional structure which is cultured in vitro, contains cells to be transplanted and which can be transplanted into a living body after the culture, characterized by including a scaffold layer mainly culturing a scaffold constituting the three-dimensional structure and a cell layer which is localized at least in a part of the surface of the tissue equivalent for transplantation continuously with the scaffold layer and which contains the cells to be transplanted or extra cellular matrix in a larger amount than the scaffold layer. This tissue equivalent is appropriately employed as a tissue equivalent for transplantation in a relatively large size. This tissue equivalent enables realization of prompt fixation to the neighborhood of the transplanted tissue and prevention of falling off.

The present invention provides new methods for the in vitro preparation of bioartificial tissue equivalents and their enhanced integration after implantation in vivo. These methods include submitting a tissue construct to a biomimetic electrical stimulation during cultivation in vitro to improve its structural and functional properties, and / or in vivo, after implantation of the construct, to enhance its integration with host tissue and increase cell survival and functionality. The inventive methods are particularly useful for the production of bioartificial equivalents and / or the repair and replacement of native tissues that contain electrically excitable cells and are subject to electrical stimulation in vivo, such as, for example, cardiac muscle tissue, striated skeletal muscle tissue, smooth muscle tissue, bone, vasculature, and nerve tissue.

A method for cryopreservation of a tissue equivalent whereby the viability of frozen cells and the biological activity of thawed cells are improved and the steps are simplified; and a cryopreserved tissue equivalent obtained by the method. Cells suspended in a cryopreserving solution are inoculated into a matrix and then frozen before the cells adhere to the matrix.

The invention relates to the technical field of a medical apparatus, and discloses a tissue equivalent compensator manufacturing method. The tissue equivalent compensator manufacturing method comprises the following steps: (1) according the wounded part of a patient and a ray irradiation range, modeling the dimension of the shape of the tissue equivalent compensator; and (2) performing 3D printing according to the model, and obtaining the tissue equivalent compensator. The tissue equivalent compensator manufacturing method has advantages of ultra-short manufacturing period, ultra-high yield rate and relatively low cost. The tissue equivalent compensator which is manufactured according to the tissue equivalent compensator manufacturing method improves the dosage amount for the skin and furthermore has advantages of high uniformity, no easy deformation and high fixability. Furthermore the tissue equivalent compensator improves treatment accuracy.

Quality assurance device for calibrating and testing the accuracy of movement-correlated computed tomography (“4D CT”) target-locating systems has a test unit sub-assembly adapted to be combined with a dynamic phantom system. The test unit sub-assembly has an axially and rotationally moveable test rod slideably disposed inside of a substantially hollow fixed housing. A matrix of markers, or “fiducials”, are located in the wall of the housing. A single fiducial is located near the distal end of the moveable test rod. The distal end portion of the moveable test rod is adapted to be connected to a motion actuator, which is programmed to oscillate the test rod in a predetermined pattern. When the test unit sub-assembly is inserted into a tissue equivalent phantom member, the combined sub-assembly and phantom member can then be subjected to four-dimensional imaging to generate a visual image. A visual comparison actual relative positions of the fiducials to the know positions of the fiducials in time indicates the accuracy of the 4D CT system.

The present invention focuses on an analytical model for hist and accurate scatter estimation. The present invention uses the Klein-Nishina (KN) formula as a starting point, which gives the Compton scattering differential cross-section for an interaction point. For a direct integration of the point scatter kernel over the irradiated volume, the large number of KN formulae. (e.g., amount of solid angle subtended) and rays traced required for calculating attenuation makes the computation very expensive. The present invention reduces the 3D formulation into an efficient 2D approach by integrating the KN formula along an interaction line of tissue-equivalent medium. An average attenuation length was assumed for each interaction point on the beam to reduce the number of rays traced. In the case of kilovoltage (kV) x rays as the imaging source and a small imaging field, with in-field scatter, the line integral derived, can be approximated by a compact analytical form.