The invention discloses a
linker primer combination for
library construction and a kit thereof. The
linker primer combination comprises a first
linker, a second linker, a first primer and a second primer, head and
tail parts of the first linker and the second linker contain
nucleotide sequences that can be complementarily paired and linked, the middle part of the first linker contains a
nucleotide sequence which cannot be connected to the second linker in a complementary
pairing manner, the middle part of the second linker contains a
nucleotide sequence which cannot be connected to the first linker in a complementary
pairing manner, the first primer contains a nucleotide sequence which is the same as a nucleotide sequence which is not in complementary
pairing connection with the middle part of the first linker and is connected to the second linker, both the first linker and the second linker do not contain a sample index, and the second primer contains a
sample label and a nucleotide sequence which can be in partial complementary pairing connection with the second linker. According to the linker primer combination and the kit, the short joint sequence without an index is used, such that the connection efficiency is significantly improved, the synthesis cost is reduced, the amplification primers are lengthened, and the amplification efficiency is effectively improved.