The invention discloses a kit for detecting B-raf
gene V600E
mutation, and a detection method thereof. The kit comprises B-raf
gene V600E
mutation detection mixed liquid, wherein the B-raf
gene V600Emutation detection mixed liquid comprises a B-raf gene V600E
mutation detection specific primer pair, a B-raf gene specific probe, a competitive inhibition
nucleotide sequence,
thermostability DNA (deoxyribonucleic acid)
polymerase,
thermostability restriction enzyme, reaction
buffer solution and reaction
enhancer. The kit has the beneficial effects that an ARMS-PCR (Amplification
Refractory Mutation System-
Polymerase Chain Reaction)
fluorescence probe technology, a competitive inhibition Blocker technology, an
oligonucleotide modification technology and a TspRI
restriction enzyme technology are subjected to combined
adaptation, mutation samples and wild samples can be more favorably distinguished so as to realize detection sensitivity and specificity better than similar technologies or products, B-raf gene V600E site mutation can be quickly, conveniently, sensitively and economically detected, and the kit is suitable for popularization application and industrialization.