The invention discloses semi-quantitative
rapid detection test paper for
immunoglobulin G (IgG) in bovine
colostrum and products thereof and a preparation method thereof, which relate to the semi-quantitative detection test paper for the IgG and the preparation method thereof and solve the problems of the complex detection operating procedure, long detection time and unsuitability for field operation of IgG in the conventional bovine
colostrum and the products thereof. The test paper consists of a back lining, a sample absorbing pad,
glass fiber, a
cellulose nitrate membrane and a water absorbing pad, wherein the
cellulose nitrate membrane is provided with 1 to 4 detection lines and 1
quality control line. The method comprises the following steps of: 1, preparing a rabbit anti-bovine IgG-
colloidal gold marker and enveloping the marker on the
glass fiber; 2, enveloping the detection lines and the
quality control line on the
cellulose nitrate membrane; and 3, assembling the test paper. The test paper has the advantages of high detection flexibility, minimum detectable amount of 10mu g / mL, high specificity, no cross reaction with IgG in ovine
colostrum and products thereof, simple procedure, high detection speed, detection time of generally 15 to 20 minutes for one sample and suitability for
field detection.