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Colloidal gold test strip for combined detection of procalcitonin (PCT)/C-reactive protein (CRP) and preparation method thereof

A colloidal gold test strip and combined detection technology, applied in the field of clinical medical examination, can solve the problem of not being able to detect PCT/CRP at the same time, and achieve the effect of simple operation and accurate results

Inactive Publication Date: 2012-10-03
GETEIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a colloidal gold test strip for combined detection of PCT / CRP against the deficiencies of the prior art, which can solve the problem that PCT / CRP cannot be detected simultaneously in one test strip in the prior art

Method used

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  • Colloidal gold test strip for combined detection of procalcitonin (PCT)/C-reactive protein (CRP) and preparation method thereof
  • Colloidal gold test strip for combined detection of procalcitonin (PCT)/C-reactive protein (CRP) and preparation method thereof
  • Colloidal gold test strip for combined detection of procalcitonin (PCT)/C-reactive protein (CRP) and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Such as figure 1 As shown, the colloidal gold test strip for PCT / CRP joint detection includes the test strip backing 1, and the first layer of sample pad 2 and the second layer of sample pad 3 that are sequentially overlapped and pasted on the bottom of the test strip 1. Polyester film 4 coated with gold-labeled antibody, coating film 5 and absorbent paper 6. In the middle of coating film 5, there is a detection line 7 coated with mouse anti-PCT monoclonal antibody 16B5, and a line coated with mouse anti-CRP monoclonal antibody DF92 A test line 8 and a control line 9 coated with rabbit anti-mouse IgG antibody. The above antibodies were purchased from Fitzgerald Company.

[0032] The above detection line 7 can also be a polyclonal antibody coated with double anti-PCT, and the detection line 8 can also be a mouse polyclonal antibody against CRP. The polyclonal antibody is prepared by immunizing mice with conventional methods known to those skilled in the art to obtain a...

Embodiment 2

[0055] PCT blood sample test:

[0056] Serum samples containing PCT with different fixed values ​​were measured by a Roche E170 chemiluminescence instrument equipped with the detection kit of the present invention, and the difference between the degree of color development and the reference serum was compared. The results are shown in Table 1.

[0057] Table 1

[0058] 0.12ng / ml 2ng / ml 10ng / ml 25ng / ml 50ng / ml PCT detection line + ++ +++ ++++ ++++ CRP detection line — — — — —

[0059] CRP blood test:

[0060] The Roche E170 chemiluminescence instrument was matched with the detection kit of the present invention to measure the serum samples containing different fixed values ​​of CRP, and the difference between the degree of color development and the reference serum was compared. The results are shown in Table 2.

[0061] Table 2

[0062] 5mg / L 20mg / L 40mg / L 80mg / L 120mg / L CRP detection line + ++ +++ ++...

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Abstract

The invention belongs to the field of clinical medical examination, particularly relates to a colloidal gold test strip for the combined detection of procalcitonin (PCT) / C-reactive protein (CRP). The colloidal gold test strip comprises a test strip bottom lining, and a sample pad, a polyester film on which gold-labeled antibodies are coated, a coating film and absorbent paper which are sequentially overlapped with and adhered to each other on the test strip bottom lining, wherein the coating film is provided with a control line which coats a rabbit antimouse immunoglobulin G (IgG) antibody, and two detection lines which are parallel to the control line and coat an antibody which can be in specific binding with a to-be-detected antigen PCT and an antibody which can be in the specific binding with a to-be-detected antigen CRP respectively; and two kinds of gold-labeled antibodies are provided, namely the antibody which is labeled by colloidal gold and can be in the specific binding with the to-be-detected antigen PCT and the antibody which is labeled by the colloidal gold and can be in the specific binding with the to-be-detected antigen CRP. By the colloidal gold test strip, the PCT / CRP can be detected simultaneously, the accuracy of diagnosing an inflammatory reaction is improved, and the gold test strip is easy to operate.

Description

technical field [0001] The invention belongs to the field of clinical medical examination, and in particular relates to a colloidal gold test strip for PCT / CRP joint detection and a preparation method thereof. Background technique [0002] Procalcitonin (PCT) is a precursor of calcitonin without hormonal activity. It is a glycoprotein composed of 116 amino acids and a molecular weight of 13KD. PCT is secreted and expressed by intraneural cells (including thyroid, lung and pancreatic tissue cells), and is decomposed into calcitonin (CT), carboxy-terminal peptide and amino-terminal peptide by enzymatic digestion. The half-life of PCT in the human body is 25-30h, with good stability, and its content in normal human serum is extremely low. [0003] In many conditions leading to systemic inflammatory response syndrome (SIRS), such as bacterial infection, pancreatitis, burns, and multiple injuries, the cleavage products of CT propeptide substances in the blood are abnormally elev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 苏恩本颜彬王勇黄力陈伟孔婷婷
Owner GETEIN BIOTECH
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