749 results about "Colloidal gold test" patented technology

The invention relates to a multi-channel portable colloidal gold test paper strip quick test device, which mainly comprises a detector, wherein the detector comprises a detecting part, an electrical part and a software detecting part; the detecting part comprises a plurality of mutually independent detecting channels; the electrical part comprises an ARM data processor, a keyboard and an LCD; image data detected by the detecting part are processed and displayed by the electrical part; and the software detecting part controls detecting steps. The device has a multi-channel detecting structure, and four channels can carry out the same detection item as well as different detection items. The device has the advantages of high detection speed, large amount of samples and great practical value.

The invention discloses a monoclonal antibody 64360-52D1 capable of specifically combining with SARS-Cov-2 virus structural nucleocapsid protein (N protein), a preparation method thereof, and 6 complementarity-determining regions (CDR) of heavy chain and light chain variable regions; and more specifically, the monoclonal antibody is secreted by a hybridoma strain 64360#52D1, and can specifically recognize the SARS-Cov-2 virus N protein rather than SARS virus N protein. Thus, the monoclonal antibody can be used for identifying the two coronaviruses with high similarity. The invention further provides an enzyme-linked immunosorbent assay (ELISA) and an immune colloidal gold test strip detection method for specifically detecting the SARS-Cov-2 virus N protein by preparing the 64360#52D1 antibody. The antigen of the antibody is SARS-Cov-2 virus N protein subjected to heat treatment and expressed in mammalian cells; the finally obtained antibody belongs to an IgG1 subtype; and a sequence for encoding the variable region of the antibody is obtained in a gene cloning mode.

The invention provides a piece of colloidal gold test paper comprising an information storage unit. The colloidal gold test paper is characterized in that the information storage unit stores test paper detection information which can be read by an external detection unit; and the test paper detection information is used for controlling the external detection unit to complete the detection process. With the adoption of a method above, the colloidal gold testing method can be standardized and processized; and a colloidal gold detection card reading machine can be used for automatically identifying different pieces of colloidal gold test paper and automatically completing the detection process.

The invention discloses a kit and a method for detecting sST2 (soluble ST2) in blood of an abdominal aortic aneurysm and / or aortic dissection patient. According to the kit and the method, a pair of antibodies for identifying different epitopes of sST2 is assembled to obtain a double-antibody sandwich ELISA and immune colloidal gold test strip, so as to realize quantitive and qualitative detection of sST2. Tests prove that the ELISA kit and the immune colloidal gold test strip have relatively high specificity and sensitivity in an abdominal aortic aneurysm marker-sST2 protein of human, are simple to operate, can be utilized for detecting abdominal aortic aneurysm, aortic dissection and other diseases in scientific research and clinical application, and have the functions of auxiliary diagnosis, guide treatment and prognosis judgment.

The invention discloses a kit used for rapid detection of enterobacter sakazakii in milk, and applications thereof, and belongs to the field of food safety technology. The kit comprises a loop-mediated isothermal amplification (LAMP) reaction system, Bst DNA polymerase, a probe and a colloidal gold test strip. The kit is capable of solving a problem that result interpretation of existing LAMP detection methods is not capable of realizing on-site detection; result interpretation method of the test strip of the kit is simple, no instrument is needed, and interpretation can be realized by the naked eye.

The invention relates to a novel, quick, high-sensitivity, qualitative and quantitative clinical test method which uses a chemically modified functional polymorphic substrate as media and combines inorganic fluorescent semiconductor nano materials (quantum dots) and immunology means. The multi-functional quick fluorescence immunoassay test method using a functional substrate as media and using single color and multi-color quantum dots as a mark is suitable for a single or compound qualitative and quantitative test of various hormone, protein, nucleic acid and pathogens. Compared with traditional enzyme-linked immunoassay and colloidal gold test paper, the multi-functional quick fluorescence immunoassay test method has the advantages of being simple, quick, high in sensitivity, long in storage time and the like. The multi-functional quick fluorescence immunoassay test method can meet requirements of different testing conditions and samples, can conduct the single or compound qualitative and quantitative test on different antigens on the same substrate media at the same time, and has great potential on aspects of clinical applications.

The invention discloses an immune colloidal gold test strip for detecting porcine epidemic diarrhea virus as well as a preparation method and application thereof. The test strip sequentially comprises a sample pad, a colloidal gold pad, a nitrocellulose membrane, an absorbent paper and a PVC (poly vinyl chloride) base plate arranged below and used as an assembling platform according to a connection sequence, wherein the colloid gold pad comprises a glass cellulose membrane of a PEDV (porcine epidemic diarrhea virus) monoclonal antibody adsorbed with colloidal gold marks, the nitrocellulose membrane is provided with a goat rat resisting IgG (immunoglobulin G) polyclonal antibody coated quality control line and a rabbit resisting PEDV S protein polyclonal antibody coated detection line, and the PEDV monoclonal antibody is generated in secretion of hybridoma cells with a preservation number CCTCC C201392. Experiments prove that the test strip has the advantages of strong specificity and good stability; the operation is simple, technicists do not need special training, no special equipment is needed, the detection cost is low, the detection speed is fast and the results can be read in 5-10 minutes, and the test strip is applicable to field test.

The invention discloses an immune colloidal gold indicator paper and making method to test cow tuberculosis antibody, which comprises the following parts: sample pad, connecting pad, cellulose nitrate film, absorbent pad and PVC backing liner, wherein the sample pad, connecting pad, cellulose nitrate film and absorbent pad are attached on the PVC backing liner; the connecting pad clads MPB83 protein-colloidal gold marker; the purified MPB70 protein is cladded on the cellulose nitrate film, which constitutes detecting line and quality control line formed by pure rabbit-anti-MPB83 protein lgG. The invention also provides the making method of cow mycobacterium astopic antigen MPB83, which possesses the recombinant Escherichia coli BL21 / pET28a-MPB83 in the CCTCC with reserving number at CCTCC NO:M206142.

The invention discloses an immune colloidal gold reagent used for detecting tobacco virus series and preparing method thereof, comprising an immune colloidal gold test strip used for detecting TMV virus and preparing method thereof, an immune colloidal gold test strip used for detecting CMV virus and preparing method thereof and an immune colloidal gold test strip used for detecting PVY virus and preparing method thereof; the preparing method has simple production process and low cost; the products have high sensitivity and good repetitiveness; the cost used for detection is only 1 / 5 to 1 / 10 of that of the RT-PCR and is much cheaper than an enzyme-labeled reagent kit; the detection has fast speed with the whole process being only 30 minutes, can be carried out on spot, and has the advantages of accurate result, fast speed, simple operation and easy promotion and use.

The invention discloses a Cpf1 reagent kit for quickly detecting nucleic acid of an African swine fever virus. The Cpf1 reagent kit comprises a Cpf1 detection system suitable for quickly detecting theAfrican swine fever virus, and an immune colloidal gold test strip, wherein the Cpf1 detection system comprises specific crRNA protein, specific Cpf1 protein and a single-chain DNA(ssDNA) reporting system in accordance with a p72 gene of the African swine fever virus, the specific crRNA is one or more of crRNAs from ASFV P72 crRNA1 to ASFV P72 crRNA10, and the sequence of the specific crRNA is SEQ NO.4 to SEQ NO.13; and the single-chain DNA(ssDNA) reporting system comprises ssDNA FQreporter for fluorescence detection of a microplate reader and / or ssDNA DB reporter for detecting the immune colloidal gold test strip. According to the Cpf1 reagent kit disclosed by the invention, for the first time, the Cpf1 is used for detecting the African swine fever virus, and has the advantages of beinghigh in sensitivity, high in specificity, short in time consumption, high in flux, independent of large-scale experiment equipment and the like. The advantages enable a detection method based on the immune colloidal gold test strip developed by the invention to be conveniently used in basic laboratories and breeding enterprises to be used for performing detection, identification and diagnosis on basic quick detection of the African swine fever.

This invention discloses one immune glue gold test bar and process method for organophosphorus in agriculture test technique field, wherein, the test bar comprises PVC underlay, adhesive agent layer, sample pad, combination pad, aqua fortis fiber film, weight band, test band, compare band, overlap band, water absorptive pad, limit line and arrow line. The process method comprises the following steps: processing the medicine BSA load protein coupler; processing the medicine special signal clone antigen; processing sheep, rabbit or mouse antigen IgG; processing glue gold; processing single clone antigen gold label.

The invention belongs to the field of clinical medical examination, particularly relates to a colloidal gold test strip for the combined detection of procalcitonin (PCT) / C-reactive protein (CRP). The colloidal gold test strip comprises a test strip bottom lining, and a sample pad, a polyester film on which gold-labeled antibodies are coated, a coating film and absorbent paper which are sequentially overlapped with and adhered to each other on the test strip bottom lining, wherein the coating film is provided with a control line which coats a rabbit antimouse immunoglobulin G (IgG) antibody, and two detection lines which are parallel to the control line and coat an antibody which can be in specific binding with a to-be-detected antigen PCT and an antibody which can be in the specific binding with a to-be-detected antigen CRP respectively; and two kinds of gold-labeled antibodies are provided, namely the antibody which is labeled by colloidal gold and can be in the specific binding with the to-be-detected antigen PCT and the antibody which is labeled by the colloidal gold and can be in the specific binding with the to-be-detected antigen CRP. By the colloidal gold test strip, the PCT / CRP can be detected simultaneously, the accuracy of diagnosing an inflammatory reaction is improved, and the gold test strip is easy to operate.

The invention discloses a sample pad processing liquid, a H7 subtype avian influenza virus colloidal gold test strip and a preparation method thereof, the processing liquid is prepared as follows: taking 8-20g of PEG(polyethylene glycol)-200, 11-25g of PVP (polyvinyl pyrrolidone), 10-45g of BSA (bovine serum albumin), 0.5-30ml of Triton X - 100, and 0.8-15ml of Twain-20, then using 5mM-10mM phosphate buffer solution to fix the volume to 1L, and adjusting the pH value to 9.5; and the colloidal gold test strip is formed by overlapping and pasting in turn a sample pad, a glass fiber membrane, a cellulose nitrate membrane and absorbent paper on a bottom plate, and the sample pad is processed by the processing liquid. The processing liquid can increase the sample pad hydrophilicity, contributes to the sample pad fast wetting, and promotes the chromatographic effect, aqueous solution formed by dissolving large molecules in water has suspension and dispersion effect, and can protect virus antigen in a sample. The test strip has the advantages of safe and simple operation, strong specificity, high sensitivity, and low rate of cross infection.

The invention relates to a colloidal gold test strip for semi-quantitative detection of progesterone and a preparation method thereof. The invention belongs to the technical field of dairy cow pregnancy diagnosis. Colloidal gold test strip for semi-quantitative detection of progesterone, the sample pad, the gold pad containing gold-labeled progesterone antibody complex, and the nitrocellulose membrane are pasted end to end on the bottom plate; there are detection lines and control lines on the nitrocellulose membrane . Preparation of test strips: preparation of colloidal gold: prepared from chloroauric acid and trisodium citrate; preparation of purified polyclonal antibody: preparation of artificial antigen of dairy cow progesterone by dicyclohexylcarbodiimide coupling method; artificial antigen of progesterone was then used Immune mice to prepare progesterone polyclonal antibody; prepare gold-labeled antibody: colloidal gold and antibody adsorption, centrifugal purification; colloidal gold test strip preparation: soak gold-labeled pad with Tween-containing PB solution, gold-labeled antibody perfusion glass fiber Membrane; nitrocellulose membrane Spray bovine progesterone artificial antigen and goat anti-mouse secondary antibody into 2 lines with a film dispenser. The invention is simple in operation, convenient and practical, efficient and fast, and easy to popularize.

A synthetic method of a general artificial antigen of phthalate plasticizers for immunodetection belongs to the field of bio-chemical engineering technology. The synthetic method of the present invention comprises the following steps of carrying out an esterification reaction between phthalic acid and 6-(fluorenyl methoxy carbonyl acyl-amino)-1-hexanol, removing fluorene methoxy carbonyl acyl protecting groups to form a hapten, and coupling with amino from carrier protein to obtain the general artificial antigen of phthalate plasticizers. Experimental results disclose that titer of antiserum obtained by immunizing animals by the antigen of the invention is up to 160000;the 50% inhibiting concentration IC50 for DBP, DEHP and DINP is less than 500 ng / ml; and the generated antibodies have good generality and high sensitivity. The antigen or antibodies of the invention can be used for the establishment of enzyme-linked immunosorbent assay and colloidal gold test paper rapid detection methods, thus can be used for rapid detection of residues of phthalic acid ester plasticizers in food, and have broad application prospects.

The invention relates to the field of clinical immunology, particularly to colloidal gold test paper for quickly detecting troponin I and a preparation method for the colloidal gold test paper. The colloidal gold test paper comprises a coated film, a colloidal gold pad 1, a colloidal gold pad 2, a sample pad and a water absorbing pad which are mutually attached in a staggered way; the colloidal gold pad 1 is sprayed with a colloidal gold labeled cardiac troponin I resisting antibody; the colloidal gold pad 2 is sprayed with a colloidal gold labeled BSA (Bovine Serum Albumin)-resisting monoclonal antibody or PEG (Polyethylene Glycol)-resisting monoclonal antibody; and the coated film is coated with a cardiac troponin I resisting antibody detection line and a rabbit antimouse antibody quality control line. According to the improvement on the test paper strip, a PEG resisting technology and a BSA-resisting monoclonal antibody technology are introduced into the detection of the cardiac trooping I for the first time, so that the detection sensitivity of the cardiac trooping I is greatly improved and the clinical application of a traditional colloidal gold quick diagnosis test paper is enlarged.

The invention discloses a test strip for semi-quantitative detection of microalbuminuria, belonging to medical test consumables. The test strip is characterized in that the test strip for semi-quantitative detection of microalbuminuria is a colloidal gold test strip. An anti-human albumin antibody and rabbit IgG with acolloidal gold label is enveloped on a gold label fiber pad; anti-rabbit IgG antibody is enveloped on a quality control line C, and human albumin is enveloped on a detection line T. The test strip is simple, sensitive and accurate, can be used for semi-quantitative detection of microalbuminuria in human urine and suitable for detection of early renal injury patients.

The invention relates to a colloidal gold method detection cloud platform based on mobile terminal reading, and belongs to the technical field of telemedicine. The cloud platform includes a cloud management and calculation server, a two-dimension code data server, a health record server and a mobile terminal, wherein the mobile terminal includes a camera used for obtaining image signals of a colloidal gold test board, a communication subsystem used for transmitting data tested by the mobile terminal to the cloud management and calculation server through a network as per the instruction of a main processor, and a main processor used for controlling the working conditions of the camera and the communication subsystem; and the cloud management and calculation server is used for processing the data tested by the mobile terminal and sending the received data and testing result to the mobile terminal. Through utilizing the cloud platform provided by the invention, any user with the mobile terminal can use the colloidal gold test board for detection, the testing result enables mechanisms and individuals to share the testing resources, and the testing cost can be lowered.

The invention provides a colloidal gold test strip for detecting an IgM antibody. The IgM antibody is a specific IgM antibody for nine respiratory tract infection pathogens, and the colloidal gold test strip comprises a sample pad, a conjugate pad, a nitrocellulose film and a water absorption pad which are attached to a polyvinyl chloride base plate in sequence; the conjugate pad is a glass fiber film wrapped with a rabbit-anti-human IgM antibody-colloidal gold conjugate; the nitrocellulose film is wrapped with 9 detection lines and 1 quality control line in sequence; the 9 detection lines are respectively a mycoplasma pneumoniae recombined antigen, a chlamydia pneumoniae recombined antigen, an influenza a virus antigen, an influenza B virus antigen, a sendai virus antigen, a legionella pneumophila antigen, a Coxiella burnetii antigen, a respiratory syncytial virus antigen and an adenovirus antigen, and the quality control line is a second antibody. The invention further provides a colloidal gold test strip card comprising the colloidal gold test strip and a colloid gold kit, a preparation method of the colloidal gold test strip card, and a method for realizing detection by adopting the colloidal gold test strip, the test strip card or the kit.

The present invention provides a clenbuterol monoclonal antibody and applications thereof. The present invention discloses a clenbuterol monoclonal antibody preparation method, wherein clenbuterol hapten is synthesized, the synthesized clenbuterol hapten and carrier protein are coupled to obtain clenbuterol antigen, and the clenbuterol antigen is adopted to immunize animals to obtain a high specificity monoclonal antibody. The present invention further provides a method for application of the clenbuterol monoclonal antibody in a clenbuterol enzyme-linked immunosorbent assay kit to detect clenbuterol. The present invention further provides a method for application of the clenbuterol monoclonal antibody in a clenbuterol colloidal gold test paper card to detect clenbuterol. The prepared clenbuterol monoclonal antibody has characteristics of high specificity and low cost, wherein the clenbuterol drug detection clenbuterol enzyme-linked immunosorbent assay kit prepared by using the clenbuterol monoclonal antibody and the clenbuterol drug detection clenbuterol colloidal gold test paper card prepared by using the clenbuterol monoclonal antibody have characteristics of convenient operation, high specificity, high sensitivity, high accuracy, high precision, fast detection and the like.

The invention provides an immune colloidal gold test paper card for testing quinolones, comprising a reaction film, a sample pad, a conjugate release pad, a water absorbing pad, and a back liner, wherein, the reaction film comprises a test zone coated with quinolones-carrier protein conjugate and a quality control zone coated with sheep-anti-mouse IgG; the conjugate release pad coats quinolones monoclonal antibody-colloidal gold marker. The invention also provides the method using the test paper card to test quinolones, which comprises the following steps: firstly, pre-treating sample; secondly, testing the sample with the test paper card; thirdly, analyzing test result. The invention can be used to test residual quantity of quinolones in animal derived food such as chicken, chicken liver, pork, pig liver, urine, and honey, and has the advantages of simple operation, high sensitivity, fast test, low cost, on-site supervision, and satisfaction of test requirement of a large quantity of samples.

The invention relates to a preparation method of a piece of human H-FABP colloidal gold test paper. The preparation method comprises the steps of preparing colloidal gold particles, preparing gold labelling antibody, preparing a gold labelling combination mat, coating a nitrocellulose membrane, preparing a sample processing mat, assembling the test paper and the like. According to the preparation method of the human H-FABP colloidal gold test paper, by designing a buffer solution system in the preparation process of the test paper, the problems that the detection time is long, the specificity is poor, the cost is high, and the like in the existing test paper can be solved. The invention also discloses a piece of human H-FABP colloidal gold test paper and application thereof.

The invention provides a nucleic acid aptamer-based escherichia coli O157:H7 colloidal gold test strip. The nucleic acid aptamer-based escherichia coli O157:H7 colloidal gold test strip comprises a sample pad, a colloidal gold combined pad, a nitrocellulose membrane immobilized with streptavidin, and an absorbent pad; the nitrocellulose membrane is provided with a detection line and a quality control line; the sample pad, the colloidal gold combined pad, the nitrocellulose membrane, and the absorbent pad are glued onto a plastic liner board successively; colloidal gold labeled nucleic acid aptamer is arranged on the colloidal gold combined pad; a capture probe is immobilized on the detection line; and a quality control probe is immobilized on the quality control line. The invention also provides a method used for detecting escherichia coli O157:H7 with the nucleic acid aptamer-based escherichia coli O157:H7 colloidal gold test strip. According to the nucleic acid aptamer-based escherichia coli O157:H7 colloidal gold test strip, the escherichia coli O157:H7 outer membrane protein high specificity aptamer is taken as a recognition element of test strip instead of antibodies, so that problems of immunochromatographic test strip containing antibodies, such as high cost, large batch difference, induced cross reaction, and low sensitivity, are solved, and high-efficiency rapid detection of escherichia coli is realized.

The invention discloses a preparation method and application of a product obtained by coupling melamine with carrier protein, as well as a melamine antibody. The product obtained by coupling the carrier protein with the melamine is used as artificial antigen and applied to an immunological method for melamine detection. The preparation method comprises the following steps of immunizing animals with the coupled product so as to prepare an antibody used for melamine detection, fusing BALB / C mouse spleen cells immunized with the coupled product and SP2 / 0 mouse myeloma cells, obtaining hybridoma capable of stably transferring culture and secreting anti-melamine specific monoclonal antibodies by screening positive hybridoma and cloning cells, and preparing an ascites monoclonal antibody. The prepared monoclonal antibody is utilized to establish a direct competitive ELISA method having high specificity, sensitivity and accuracy to the melamine, as well as an immune colloidal gold test strip. The preparation method for the product obtained by coupling melamine with carrier protein, as well as the melamine antibody provides service for the rapid detection of melamine-type residue in foods.

The invention relates to a colloidal gold test strip for semi-quantitatively detecting the concentration of tacrolimus drug in human whole blood and a reagent kit, belonging to the field of medical detection. A membrane reacting system of the colloidal test strip comprises a colloidal gold combining pad and a detection pad. The colloidal gold test strip is characterized in that the colloid gold combining pad is coated by anti-tacrolimus antibody marked by colloid gold; and the detection pad is provided with a detection line, and tacrolimus-carrier protein conjugate is fixed on the detection line. The detection method does not need steps of precipitating organic solvent, centrifugation and the like during detection, and can semi-quantitatively detect tacrolimus in whole blood simply, rapidly, accurately and effectively.

The invention discloses a colloidal gold test strip for fast detecting rheumatoid factors, belonging to medical test consumables. The colloidal gold test strip is characterized in that human denatured IgG with a colloidal gold label is enveloped on a gold label fiber pad; anti-mu chain IgM and human denatured IgG are enveloped on a T line, and the ratio of unit area envelop quantity of the human denatured IgG on the colloidal gold label, the anti-mu chain IgM and human denatured IgG on the T line on the gold label fiber pad is 0.133-0.889 to 5 to 1. The test strip uses an antibody capture method and a double antigen sandwich method principle during detection, has sensitivity and specificity, can fast detect the rheumatoid factors in human serum, plasma or whole blood by combining with an immune colloidal gold technique and has the advantages of simplicity, sensitivity and accuracy.

The invention relates to a monoclonal antibody as well as a preparation method and application thereof, in particular to a monoclonal antibody of fluoroquinolone drugs and application thereof, belonging to the technical field of immunochemistry. The monoclonal antibody of fluoroquinolones 4G11' is produced from a mouse hybridoma cell strain 4G11, and the subtype of 4G11 is an IgG1 type. The invention has the advantages that the monoclonal antibody 4G11' produced from the mouse hybridoma cell strain 4G11 has high cross reaction rate with various fluoroquinolone medicines and can be produced in large quantity and used for preparing an enzyme linked immunosorbent assay kit and colloidal gold test paper for detecting the fluoroquinolone medicines, achieves the purpose of quickly and sensitively detecting the residual fluoroquinolone medicines in milk, muscle and aquatic products and provides a basis for developing a kit for detecting various residual fluoroquinolone medicines.

The invention discloses a method, a special kit and test paper strips for detecting beta-lactam antibiotics based on penicillin-binding protein. The receptor kit used for detecting beta-lactam antibiotics comprises: protein represented by SEQ ID NO: 1, enzyme labeled ampicillin and a standard substance solution, wherein the standard substance is penicillin G. The kit and colloidal gold test paper strips provided by the invention have advantages of high sensitivity, high accuracy, high precision, low cost, simple operation, short detection period, simple storage, and long shelf-life. The kit and colloidal gold test paper strips are suitable for various work units. With the kit and colloidal gold test paper strips, simultaneous and rapid detections of large batches of samples can be realized, and on-site high flux rapid detections can be realized. Therefore, the kit, the colloidal gold test paper strips and the method provided by the invention play an important role in the detections of beta-lactam antibiotics.

The invention relates to a polyclonal antibody for detecting the residue and the degradation of a CP4-EPSPS protein in a processed food and application thereof. An animal is immunized by using a CP4-EPSPS protein peptide segment of a coupled keyhole limpet hemocyanin (KHL) to obtain antiserum, namely the antibody of the invention. The application comprises a Western blotting and a colloidal gold test strip and has the characteristics of simplicity, practicability, high sensitivity and the like, wherein the Western blotting is used for detecting the residue and the degradation of the CP4-EPSPS protein in food; and test strip technology is used for the rapid detection of the CP4-EPSPS protein in the food. The invention provides a high-practicability method for the safety detection of a genetically modified food, and has a very high practical application value.