206 results about "Sars virus" patented technology

The present invention provides design and synthesis of oligomeric compounds and compositions that can be administered to reduce the activity of SARS virus in vivo or in vitro, to prevent or treat SARS virus-associated disease, to detect AARS virus, and to diagnose SARS virus-associated diseases.

The present invention provides design and synthesis of oligomeric compounds and compositions that can be administered to reduce the activity of SARS virus in vivo or in vitro, to prevent or treat SARS virus-associated disease, to detect AARS virus, and to diagnose SARS virus-associated diseases.

The present invention belongs to the field of biomedicine, and relates to polypeptides capable of inhibiting human coronavirus infections, particularly to polypeptides capable of inhibiting human coronavirus infections in a broad spectrum manner, and applications thereof. According to the present invention, the polypeptides capable of providing broad spectrum inhibition effects for infections caused by more than 2 human coronaviruses are provided based on the conservation property of the S2 region of a coronavirus S protein and the similar fusion mechanism; the test results show that the polypeptides can achieve the commonality of the human coronavirus, ie., the similar HR region and the same fusion mechanism mediated by the coronavirus S protein so as to provide a series of HCoV-EK polypeptides, wherein the polypeptides provide good inhibition effects for currently popular human coronaviruses, and further provide good inhibition activity for SARS virus (RsSHC014-CoV or RsW1V1-CoV) possibly infecting human; and the polypeptides of the present invention can provide the prevention and treatment candidate drug for the currently popular human coronaviruses and the novel human coronavirus possibly emerging in the future.

The invention provides a method for permanently embedding an identity card number into a genome. The method comprises the following steps: permanently embedding an exogenous gene segment containing an identity card number into a genome by using CRISPR technology; and separating and reading specific DNA containing the identity card number. Thus, identity can always be identified via the permanently preserved DNA even an external packaging label is lost or wrong. The method is also applicable to preservation of microbes (including bacteria and viruses). For example, if a deactivated SARS virogene specimen is left, later generations can research on SARS viruses.

This invention relates generally to the field of virus detection. In particular, the invention provides chips, probes, primers, kits and methods for amplifying and detecting SARS-CoV nucleotides sequence. The clinical and other uses of the present chips, probes, primers, kits and methods are also contemplated.

The invention is confluens protein of SARS virus S protein and N protein, and the manufacturing, application and the gene project technology, vaccine and diagnosing reductant. Through analyzing the cistine series of SARS s protein and N protein, sifts out SARS virus S protein segment with strong antigen epitope, namely the 162nd cistine to 460nd cistine, and the N protein segment of SARS virus with strong antigen epitope, anmely the first cistine to 258nd cistine. It synthesizes the new SARS virus S protein segment and N protein segment gene series, connects the two gene segments in series. Expresses the confluens protein of the two protein segments, the two are connected by glycine and serine, inserts a glycine adjoining to the frist cistine methionine of N protein segment, the confluens proteiní» length is 560 cistines.

The invention discloses a monoclonal antibody 64360-52D1 capable of specifically combining with SARS-Cov-2 virus structural nucleocapsid protein (N protein), a preparation method thereof, and 6 complementarity-determining regions (CDR) of heavy chain and light chain variable regions; and more specifically, the monoclonal antibody is secreted by a hybridoma strain 64360#52D1, and can specifically recognize the SARS-Cov-2 virus N protein rather than SARS virus N protein. Thus, the monoclonal antibody can be used for identifying the two coronaviruses with high similarity. The invention further provides an enzyme-linked immunosorbent assay (ELISA) and an immune colloidal gold test strip detection method for specifically detecting the SARS-Cov-2 virus N protein by preparing the 64360#52D1 antibody. The antigen of the antibody is SARS-Cov-2 virus N protein subjected to heat treatment and expressed in mammalian cells; the finally obtained antibody belongs to an IgG1 subtype; and a sequence for encoding the variable region of the antibody is obtained in a gene cloning mode.

The invention provides arrays and probes for resequencing a SARS virus using an array of probes that are complementary to a SARS reference sequence and to each possible single nucleotide substitution of the reference sequence. Methods of identifying mutations in viral sequences and methods of characterizing viral isolates are also provided. The invention also provides high throughput methods to monitor epidemics and pandemics caused by pathogens such as viruses.

This invention relates generally to the field of virus detection. In particular, the invention provides chips, probes, primers, kits and methods for amplifying and detecting SARS-CoV nucleotides sequence. The clinical and other uses of the present chips, probes, primers, kits and methods are also contemplated.

The invention provides a novel coronavirus antigen epitope and application thereof. A super computer is used for simulating S, E, M and N protein structures of the novel coronavirus, and novel coronavirus epitopes SEQ ID NO 1-38 are obtained through calculation. The epitopes have very good immunogenicity; an antibody generated by inducing a part of epitope polypeptide has the effect of neutralizing the novel coronavirus; the antigen epitope can be combined with antibodies in serum of novel coronavirus patients at home and abroad, has the potential of resisting various novel coronavirus strains, and also has the potential of identifying and applying different strains. The epitope provided by the invention can be used for (1) research and development of novel coronavirus vaccines and universal coronavirus vaccines such as MERS viruses and SARS viruses; and (2) preparing a novel coronavirus antibody, and further detecting and typing viruses and treating diseases caused by the viruses. Thenovel coronavirus antigen epitope has a wide application prospect in the aspects of prevention of coronavirus and propagation and outbreak thereof, and detection and diagnosis of virus strains.

This invention relates to Severe Acute Respiratory Syndrome associated coronavirus (SARS virus) isolated and recombinant proteins, in particular the nucleocapsid (N) protein and spike (S) protein, as well as fragments thereof and their use in the diagnosis, treatment and prevention of Severe Acute Respiratory Syndrome (SARS). The proteins and fragments carry epitopes that are specific for the SARS virus. Thus, detection methods based on these proteins or fragments as well as the monoclonal antibodies against these proteins or fragments are specific for the SARS virus.

The invention discloses a sealed one-piece exposure suit, connecting cap, coat, trousers and podotheca into a sealed whole, a mouth set on the front side of coat and trousers, a zipper set in the place of the mouth, the mask front set outside the zipper, the veil made of transparent material, one-way valve set on the cap, concave or convex groove set at the bottom of the podotheca, and air-purifying equipment set on the waist, which can make 1-100mmH2O pressure inside the exposure suit, assure the gas in the exposure suit exhausts from inside to outside on normal condition and the phenomenon that matter flows from outside to inside does not happen, except that purified gas normally is pumped in the exposure suit, and avoid the pathogenic microbe infects doctors and nurses, like the infection caused by SARS virus. It can be used to prevent serious infectious disease.

The present invention relates to isolation and characterization of a class of isolated novel viruses which is the precursor of the virus causing Severe Acute Respiratory Syndrome (SARS) in humans (“hSARS virus”). The precursor virus which is a SARS coronavirus-like virus (“SCoV-like virus”) is identified to be morphologically and phylogenetically similar to hSARS virus. The present invention relates to a nucleotide sequence comprising the genomic sequence of the SCoV-like virus. The invention further relates to nucleotide sequences comprising a portion of the genomic sequence of the SCoV-like virus. The invention also relates to the deduced amino acid sequences of the SCoV-like virus. The invention further relates to the nucleic acids and peptides encoded by and / or derived from these sequences and their use in diagnostic methods and therapeutic methods. The invention further encompasses chimeric or recombinant viruses encoded by said nucleotide sequences and antibodies directed against polypeptides encoded by the nucleotide sequences. Furthermore, the invention relates to vaccine preparations comprising the SCoV-like virus, including recombinant and chimeric forms of said virus.

Lindley eupatorium herb total flavone and its pharmaceutically acceptable salt is used in preparing medicine for preventing and treating virus diseases, especially SARS. Lindley eupatorium herb total flavone is selected from quercetin, kaempferol, jaceidin, hyperoside, trifolirhizin, astragaloside or their mixture. Lindley eupatorium herb total flavone in the concentration of 100 microgram / ml has no toxicity to VeroE6 cell and complete supperssion on SARS virus.

The present invention uses the replaseable biosensor chip technology as core, and uses the related MEMS system formed from microreactor and microflow cell to make determination of environment pollution. After the harmfule molecule (for example influenza virus or SARS virus) is combined on the functional chip, said polluted molecule can store the information, and can release it in specific environment, and its released information is directly propertional to the polluted extent, so that it can implement the determination of environmental pollution.

A small-interference ribonucleic acid (SiRNA) molecule able to attack the mRNA molecular of SARS virus and its application in preparing the medicines for treating SARS and its virus infection associated diseases are disclosed. Said SiRNA is a double-stranded RNA molecule.

The present invention provides a series of artificial CpG-containing single-stranded oligodeoxynucleotides (ODNs), each of which is consisted of single-stranded oligodeoxynucleotide DNA molecule containing one or more CpG(s), wherein said ODNs can stimulate human peripheral blood mononuclear cell (PBMC) to produce antiviral substances. These ODNs can protect the cells against the attack from virus, wherein said virus is preferably selected from the group consisted of influenza virus and single-stranded positive strand RNA virus such as SARS virus, hepatitis C virus, dengue virus and Japanese encephalitis virus. Moreover, the antiviral use of artificial CpG ODNs and its use for treating and preventing viral infection are also provided.

The present invention discloses a method for clinical detection of SARS virus and its kit. According to the characteristic analysis of SARS virus genome sequence a specific region can be selected, and according to the characteristics of that said virus is of ribonucleic acid virus a specific primer sequence can be used and the reagent and techniques related to RT-PCR and PCR can be adopted so as to create a set of methods for quickly detecting SARS virus. Said invented method has higher accuracy, and provides its diagnosis method and its kit.

The detection process of SARS virus of the gene detection reagent kit with rationing standard specific SARS virus nucleic acid as reference includes RNA extraction, PCR amplification and fluorescent detection. The venous blood sample, gargled liquid or respiratory tract secretion of the patient is used as the analysis sample directly, RNA of the sample is extracted as the nucleic acid template, and the template is used in fluorescent PCR amplification. The used fluorescent amplification detecting reagents includes one-step process RT-PCR buffering liquid, deoxynucleoside triphosphate (dNTP) mixture, specific amplification primer and specific probe. The method of the present invention is fast and convenient in detecting SARS virus.

The invention relates to a banknote counter sterilizer and a sterilization method thereof. By means of the sterilizer, the banknotes go through a linear sterilization bin in a shape of U thwarted rightward with two-sided ultraviolet irradiation and are efficiently sterilized in both sides when counted at high speed. The sterilizer has no harm to the banknotes and is safe and convenient in use. Moreover, the sterilizer is equipped with an air wet sterilization purifier, which can efficiently purify the interior of the sterilizer and the indoor air and can be applied to ordinary banknote counter, thus upgrading the technology thereof. The sterilizer solves the difficulty of the synchronization of banknote counter speed and banknote sterilization, filling the blank at both home and abroad, and can effectively kill various pathogenic microorganisms tainted on the banknotes such as SARS virus, flu virus and tubercle bacillus, with high sterilizing rate reaching the national and international disinfection and sterilization standards; the sterilizer of the invention can be widely applied to the disinfection of banknotes received by banks, post and telecommunications, railway stations, subway stations, bus stations, airports, stores, hotels and restaurants as well as to the banknote treatment of customs, hospitals, areas of infectious diseases and epidemic victims, thereby being beneficial to the prevention of pestilence and to the health of people.

Cepharanthine is used as effective component in preparing medicine for resisting SARS virus. Within the safe dosage range, the present invention has high inhibition rate to SARS virus and powerful effect of preventing SARS virus infection. The medicine can alleviate the syndromes of SARS patient and has fast after cure, less toxicity, high stability and other advantages.

A Chinese medicine in the form of oral liquid, injection, spray, instant particles, tablet, or capsule for preventing and treating SARS is prepared from the raw materials including common goldenrod herb. Its advantage is high effect on killing and suppressing SARS virus and coronavirus.

A SARS-resistant suit of clothes with antifogging, temp regulating water-drinking, sweat-discharging, urine-discharging, talking and safety helmet functions is composed of the cylindrical head-piece with semi-spherical top, which contains toxin filter, electronic antifogging device, temp regulator, safety belt, microphone, loudspeaker, water supplying tube, oxygen supplying inlet, etc. the SARS-resistant coat and trousers able to discharge sweat, urine discharging device, SARS-resistant shoes, urine container, and controller.

This invention relates to a detection mode of SARS virus antibody and a preparing method of the box for rapid diagnosis reagent. The checking method includes that (1) putting antihuman Ig antibody marked by colloidal gold in the carrier, and coating detecting thread made of variant antigen of SARS coronavirus and controlling thread made of antipest IgG antibody in the detecting carrier connecting with the marked one. (2) putting human serum in the marked carrier. The box for rapid diagnosis reagent includes checkerboard marked by variant antibody of SARS coronavirus, which comprises water-absorbing layer of adding-kind edge, detecting layer and water-absorbing layer of water-uptake edge. There is antihuman Ig antibody layer between detecting layer and water-absorbing layer of water-uptake edge, and in the detecting layer there is detecting thread made of variant antigen of SARS coronavirus and controlling thread made of antipest IgG antibody coating.

The present invention provides a method for measuring SARS virus nucleocapsid protein (SARS-NP) using first and second antibodies both binding specifically to SARS-NP, wherein the first or second antibody is an antibody recognizing an epitope located in a region (Region C) of amino acid 283 to 422 from the N-terminus of the amino acid sequence of SARS-NP.

The present invention relates to the field of biological engineering technology, specifically, to a SARS virus vaccine with adenovirus carrier, preparation method thereof and use of SARS virus S gene for preparation of severe acute respiratory syndrome (SARS) virus vaccine.

The washing skin care article consists of extractives of honeysuckle and skullcap root, and medium. The products of the present invention has skin cleaning and protecting effect, and inhibiting and killing effect on various kinds of germs and viruses, and especially it has relatively high inhibiting effect on coronavirus including SARS virus. The products of the present invention include washing article, cosmetics, wet tissue, toothpaste, etc. The present invention makes it possible to prevent the invasion of various bacteria and viruses to human body and to prevent infectious diseases.

The invention provides a primer and probe combination for detecting beta coronavirus. The primer and probe combination comprises primers and probes aiming at a beta coronavirus A general system, a beta coronavirus B general system, a beta coronavirus C general system, a beta coronavirus C general system MERS and / or a beta coronavirus D general system. The invention also provides an application ofa 18S rRNA sequence as a reference gene in virus detection of mammals and poultry. The invention further provides a kit for integrally detecting the beta coronavirus in animals, and the kit comprisesa reaction solution packaged by the kit, and further comprises a cracking extracting solution and a cleaning solution. According to the invention, through the design of the primers and probes, animal-derived beta coronaviruses such as novel coronaviruses SARS-CoV-2, SARS viruses and MERS viruses can be simultaneously detected in one reaction, so that complete coverage of three major high-infectivity respiratory system pathogens is realized in one reaction, and the kit and the method have relatively high application value for tracing animal souces of the three major high-infectivity respiratorysystem pathogens.

The invention discloses novel specific polypeptide, which comprises 114 novel specific polypeptides, and is characterized in that the novel specific polypeptide has a length of about 8 to 20 amino acids, can establish a model of an SARS virus polypeptide sequence, can react with an antibody in human serum produced by the irritation of SARS viruses and the like. The novel specific polypeptide can be used for preparing a medicament for diagnosing, treating and preventing severe acute respiratory syndrome caused by coronaviruses.