SARS virus antibody detecting method, rapid diagnosis kit and preparation method
A technology for rapid diagnosis and antibody detection, applied in the field of diagnosis and detection of SARS coronavirus antibodies, can solve the problems of difficulty in virus antigen preparation, inability to mass-produce, poor specificity, etc., and achieve the effects of simple preparation method, simple operation and good stability.
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Embodiment 1
[0030] A kind of SARS coronavirus mutant strain antibody detection method comprises the steps: (1) the anti-human Ig antibody of colloidal gold labeling comprises IgG and IgM to be carried on glass fiber or non-woven cloth carrier: and with colloidal gold labeling antibody The detection carrier that is connected to the carrier is coated with the detection line formed by the genetically engineered SARS coronavirus variant antigen and the control line formed by the anti-mouse IgG antibody: (2) get human serum and drop it on the carrier of the colloidal gold-labeled antibody, if the serum contains The SARS coronavirus mutant antibody forms a detection line and a control line on the detection carrier, which is positive, otherwise it is negative.
[0031] refer to figure 1 A gold-labeled rapid diagnostic kit for antibodies to variants of SARS coronavirus is equipped with a gold-labeled detection plate 1 for antibodies to variants of SARS coronavirus.
[0032] refer to figure 2 ,...
Embodiment 2
[0038] The SARS coronavirus variant antibody detection method and its gold-labeled rapid diagnostic kit are included with the example.
[0039] The preparation method of the kit is the same as the example except that the colloidal gold-labeled antibody is an anti-human IgM monoclonal antibody.
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