213 results about "Coronavirus antibody" patented technology

The invention discloses a middle east and respiratory syndrome coronavirus antibody and a preparation method thereof, and belongs to the technical field of cell-mediated immunity. RDB protein of an MERS-CoV virus S protein receptor binding domain is obtained by using in-vitro expression and purification of a baculovirus expression vector system, and Bab / c mice are immunized by using high-purity MERS RBD protein, an efficient antiviral neutralizing anti-body is screened by the methods such as an enzyme-linked immuno sorbent assay (ELISA), a false virus neutralization experiment and the like. The antibody and the crystal structure of the MERS RBD protein are analyzed, and a crucial amino acid of the antibody combined with viral proteins is determined from the molecular level. Thus, the antibody is further humanized, and the possibility is provided for clinical detection, prevention and treatment of MERS-CoV virus infection.

The invention discloses a novel fluorescence immunochromatography test strip for joint inspection of SARS-CoV-2 IgG-IgM antibodies of coronaviruses. The test strip comprises a bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad, the combination pad, the nitrocellulose membrane and the water absorption pad are sequentially connected end to end and adhered to the bottom plate; the combination pad is coated with an SARS-CoV-2 structural protein-marker and a goat anti-rabbit IgG-marker, and the nitrocellulose membrane is provided with a detection line T1 coated with a mouse anti-human IgG monoclonal antibody, a detection line T2 coated with a mouse anti-human IgM monoclonal antibody and a quality control line C coated withrabbit IgG. When the test strip is used for quantitatively detecting SARS-CoV-2 IgG and IgM antibodies, the detection sensitivity is high, and the specificity is good and can reach 96%; the batch-to-batch difference is small, and good repeatability is achieved; the test strip can be stored for half a year at normal temperature without reducing the sensitivity and has good stability; the test strip is simple to operate and low in cost, can quickly and quantitatively detect the levels of SARS-CoV-2 IgG and IgM antibodies in a human body, assists a nucleic acid detection means, and provides powerful support for epidemic situations.

The invention relates to a neutralizing antibody of coronavirus or an antigen-binding fragment thereof, a nucleic acid molecule encoding the antibody or the antigen-binding fragment thereof, a vectorcontaining the nucleic acid molecule, a host cell containing the vector, application of the antibody or the antigen-binding fragment thereof in preparation of drugs for treating or preventing diseasescaused by coronavirus, and application in detection of products. The coronavirus antibody and the antigen-binding fragment thereof have broad-spectrum and strong neutralizing ability for various coronaviruses including SARS-CoV-2, and have good clinical application prospects in the future.

The invention relates to the technical field of in-vitro diagnostic reagents, in particular to a kit for combined detection of novel 2019 coronavirus IgM and IgG antibodies and a preparation method ofthe kit. The kit comprises a duplex detection card and a detection buffer solution; a 2019 novel coronavirus IgM antibody detection test strip and a 2019 novel coronavirus IgG antibody detection teststrip are arranged in the duplex detection card; the 2019 novel coronavirus IgM antibody detection test strip and the 2019 novel coronavirus IgG antibody detection test strip respectively comprise asample pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad is coated with an anti-sheep erythrocyte antibody, and the nitrocellulose membrane is provided with a fluorescent antibody solidus, a detection line and a quality control line. By using the kit, the detection rate can be improved, and the operation is simple.

The invention relates to a recombinant protein and test strip for detection of antibodies of 2019 novel coronavirus through a double-antigen sandwich method and a preparation method and application ofthe recombinant protein and test strip, and belongs to the technical field of virus detection. The amino acid sequence of the recombinant protein for detection of the antibodies of the 2019 novel coronavirus through the double-antigen sandwich method is shown in SEQ ID NO. 1. The recombinant protein is a fusion protein of multiple dominant epitopes of the 2019-nCoV, a reagent for detection of theantibodies of the 2019-nCoV through the double-antigen sandwich method can be prepared, storage at room temperature, fast and single detection with high sensitivity, high throughput and low instrument cost can be achieved at any time, the operation is simple, and convenience of clinical use can be improved greatly.

The invention relates to a method for detecting novel coronavirus 2019-nCoV through multi-protein joint inspection combination, which belongs to the technical field of novel coronavirus 2019-nCoV detection. The invention provides the method for detecting the novel coronavirus 2019-nCoV through multi-protein joint inspection combination. A detection test strip for detecting the novel coronavirus 2019-nCoV antibody is prepared by directly scribing a high-purity and specific novel coronavirus antigen on a nitrocellulose membrane, and IgG, IgM and / or IgA antibodies in a sample can be simultaneously detected by utilizing multiple antigens. Through joint inspection of three or four specific antigens, protein sequences of the antigens are optimally designed, so that the sensitivity and specificity are effectively improved, and the problems of false negative and false positive are effectively avoided.

The invention discloses a fluorescence detection test strip for a canine coronavirus antibody as well as a preparation method and application thereof. The test strip provided by the invention comprises a sample absorption pad, a conjugate release pad a, a conjugate release pad b, a nitrocellulose membrane, a water absorption pad and a bottom plate, wherein a chicken anti-dog anti-antibody-biotin conjugate is sprayed on the conjugate release pad a; a streptavidin-fluorescent microsphere marker is sprayed on the conjugate release pad b; a detection line and a quality control line are arranged onthe nitrocellulose membrane; the detection line is coated with a canine coronavirus N expression protein; and the quality control line is coated with a sheep anti-chicken anti-antibody. The test strip disclosed by the invention has the advantages of high sensitivity, high specificity, easiness in operation, economical efficiency and practicability, and can realize field rapid quantitative detection of the canine coronavirus antibody.

The invention provides a recombinant protein and test paper for detecting a novel coronavirus 2019-nCoV IgA antibody, and a preparation method and application, and belongs to the technical field of virus detection. The amino acid sequence of the recombinant protein is shown in SEQ ID No.1; the test paper comprises a bottom plate as well as a sample absorption pad, a fluorescent microsphere pad, anitrocellulose membrane and a water absorption pad which are in sequential lap joint and adhesion to the bottom plate; a 2019 novel coronavirus specific antigen marked by fluorescent microspheres is sprayed to the fluorescent microsphere pad; a detection zone and a quality control zone are fixed on the nitrocellulose membrane; an anti-human IgA antibody is sprayed to the detection zone; and a sheep anti-chicken IgY antibody is sprayed to the quality control zone. By detecting the IgA antibody in a saliva sample, the test paper provided by the invention is capable of simply, rapidly and accurate detecting novel coronaviruses, and early-stage detection on the novel coronaviruses can be achieved.

The invention provides a test strip and a kit for detecting a human novel coronavirus IgM antibody and a preparation method of the test strip and the kit. A nitrocellulose membrane of the test strip is provided with a detection line and a quality control line; the detection line is coated with novel coronavirus nucleocapsid protein and S1 protein, and the quality control line is coated with a chicken IgY antibody; the combination pad of the test strip is coated with a quantum dot labeled goat anti-human IgM antibody and a quantum dot labeled goat anti-chicken IgY antibody. According to the test strip provided by the invention, novel coronavirus N protein and S1 protein are simultaneously coated on the detection line; the goat anti-human IgM antibody and the goat anti-chicken IgY antibody are labeled by using quantum dots; the human IgM antibody is a serum specific antibody firstly appearing after being infected with novel coronavirus; and the obtained test strip can be used for effectively and accurately detecting patients infected with the novel coronavirus at the early stage, and has important significance for preventing further diffusion of the virus.

The invention discloses a preparation method of a novel coronavirus 2019-nCoV IgM / IgG antibody combined detection kit, and belongs to the technical field of biological detection. According to the kit,latex chromatography is adopted, a PVC plate is used as a carrier, a sample pad, a compound pad, a nitrocellulose membrane and absorbent paper are sequentially overlapped and attached from left to right to form a latex test strip, and the latex test strip is loaded in a plastic clamping shell. The kit disclosed by the invention has the beneficial effects that the kit adopts latex chromatography,and IgM antibodies and IgG antibodies of the novel coronavirus 2019-nCoV in human whole blood, serum or plasma are detected by an immunocapture method. The situation that the body immune system responds to the new coronavirus can be rapidly mastered, and great reference significance is achieved for early diagnosis and post-cure reexamination of patients. In addition, the detection kit has the characteristics of high sensitivity, strong specificity, simplicity in operation, no need of complex equipment, short detection time and the like, and can play an important assistant role in eliminating the new coronavirus for human beings.