37 results about "Canine coronavirus" patented technology

This invention relates to vaccines and methods for protecting dogs against disease caused by Bordetella bronchiseptica. This invention relates to vaccines and methods for protecting dogs against disease caused by Leptospira bratislava. This invention also relates to combination vaccines and methods for protecting dogs against disease or disorder caused by canine pathogens, for example, infectious tracheobronchitis caused by Bordetella bronchiseptica, canine distemper caused by canine distemper (CD) virus, infectious canine hepatitis (ICH) caused by canine adenovirus type 1 (CAV-1), respiratory disease caused by canine adenovirus type 2 (CAV-2), canine parainfluenza caused by canine parainfluenza (CPI) virus, enteritis caused by canine coronavirus (CCV) and canine parvovirus (CPV), and leptospirosis caused by Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae or Leptospira pomona.

The invention discloses a fluorescence detection test strip for a canine coronavirus antibody as well as a preparation method and application thereof. The test strip provided by the invention comprises a sample absorption pad, a conjugate release pad a, a conjugate release pad b, a nitrocellulose membrane, a water absorption pad and a bottom plate, wherein a chicken anti-dog anti-antibody-biotin conjugate is sprayed on the conjugate release pad a; a streptavidin-fluorescent microsphere marker is sprayed on the conjugate release pad b; a detection line and a quality control line are arranged onthe nitrocellulose membrane; the detection line is coated with a canine coronavirus N expression protein; and the quality control line is coated with a sheep anti-chicken anti-antibody. The test strip disclosed by the invention has the advantages of high sensitivity, high specificity, easiness in operation, economical efficiency and practicability, and can realize field rapid quantitative detection of the canine coronavirus antibody.

The invention provides a method for labeling antibodies by colorful fluorescent granules and a test paper strip prepared from the antibodies. Animal viruses including, but not limited to, canine distemper viruses, canine parvoviruses, canine adenoviruses, canine coronavirus, rabies viruses, feline leukemia viruses, Marek's disease viruses, Newcastle disease viruses and the like are used as targets, and the corresponding antibodies labeled by colorful fluorescent micro-spheres are prepared by the aid of chemical covalent processes and can be applied to detecting the animal viruses. The method for labeling the antibodies by the colorful fluorescent granules and the test paper strip prepared from the antibodies have the advantages that colorful detection lines with bright developed colors can appear during detection, and accordingly qualitative results can be obtained; the contents of the animal viruses in samples further can be subsequently quantitatively obtained, accordingly, test results are clear and are high in stability, and the test paper strip can be stored at the room temperature for a long time.

The invention discloses an immunofluorescence chromatography detection card used for detecting a canine coronavirus antigen, and a preparation method, and aims to provide the immunofluorescence chromatography detection card which has the advantages of high sensitivity and reliable detection result and is used for detecting the canine coronavirus antigen. The immunofluorescence chromatography detection card has the technical key points that the detection card comprises a bottom plate, wherein a sample cushion, a combination cushion, a nitrocellulose membrane and a water adsorption cushion are connected in sequence onto the bottom plate; the combination cushion is provided with a CCV (canine coronavirus) monoclonal antibody labeled by fluorescent microspheres and goat anti-chicken IgY labeled by the fluorescent microspheres; the nitrocellulose membrane is provided with the quality control C line of coating chicken IgY, and the detection T line of the coating CCV monoclonal antibody. Theimmunofluorescence chromatography detection card belongs to the field of animal epidemic diseases.

The invention provides a primer set, a reagent kit and a method for detecting canine distemper viruses (CDV), canine parvoviruses (CPV) and canine coronaviruses (CCV), and belongs to the technical field of virus detection. The primer set comprises primers with nucleotide sequences shown as SEQ ID No.1-6. The primers correspond to three pairs of upstream and downstream primer pairs for the canine distemper viruses (CDV), the canine parvoviruses (CPV) and the canine coronaviruses (CCV). The reagent kit comprises the primer set. The method is based on the primer set and the reagent kit and is used for detecting the canine distemper viruses, the canine parvoviruses and the canine coronaviruses. The primer set, the reagent kit and the method have the advantages that the canine distemper viruses, the canine parvoviruses and the canine coronaviruses can be detected by the aid of the primer set, the reagent kit and / or the method, the primer set, the reagent kit and the method are good in specificity and accuracy and high in sensitivity and efficiency and are simple and speedy, reliable technical means can be provided to clinical detection, and the like.

The invention relates to a test paper strip and method for detecting a canine coronavirus through immunofluorescence chromatography technique, and specifically discloses the test paper strip for detecting the canine coronavirus through immunofluorescence chromatography technique. The test paper strip includes a marker pad containing a fluorescent-microsphere-labeled canine coronavirus antibody, and a capture membrane containing another canine coronavirus antibody. The invention further discloses the method for detecting canine coronavirus through immunofluorescence chromatography technique. The method comprises: dropwise adding a canine-coronavirus-containing solution to the sample pad of the test paper strip, and detecting a signal through a strip reader after reaction for a while.

This invention relates to vaccines and methods for protecting dogs against disease caused by Bordetella bronchiseptica. This invention also relates to combination vaccines and methods for protecting dogs against disease or disorder caused by canine pathogens, for example, infectious tracheobronchitis caused by Bordetella bronchiseptica, canine distemper caused by canine distemper (CD) virus, infectious canine hepatitis (ICH) caused by canine adenovirus type 1 (CAV-1), respiratory disease caused by canine adenovirus type 2 (CAV-2), canine parainfluenza caused by canine parainfluenza (CPI) virus, enteritis caused by canine coronavirus (CCV) and canine parvovirus (CPV), and leptospirosis caused by Leptospira Bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae or Leptospira pomona. The vaccines of the present invention include a Bordetella bronchiseptica p68 antigen.

The invention provides a reagent composition or to-be-tested substance treating fluid capable of inhibiting non-specific reactions compared with conventional immunochromatographic detection technology for a canine coronavirus. The invention also provides a high-performance high-sensitivity immunochromatographic detection apparatus and detection method using the reagent composition. When an immunochromatographic test strip is used for detecting the canine coronavirus in a sample, the sensitivity of immunoreactions can be improved and non-specificity of the immunoreactions can be lowered by using the reagent composition containing weak a diluent, a chelating agent, a surfactant and the like or a diluted solution of a to-be-tested substance for immunochromatography.

The invention relates generally to the field of virology, and relates to the identification and characterization of the targets involved in the evasion strategy of viruses and to the use thereof in methods to identify anti-viral compounds. More in particular to identify compounds which are modulators of myosin light chain kinase (MLCK), a target within said entry and immune-evasion strategy. Other aspects of the invention are directed to anti-viral compounds identified using the models and methods of the present invention, as well as to the use thereof in treating viral infections, such as for example caused by the feline infectious peritonitis virus (FIPV), a coronavirus which belongs to an antigenic group which comprises in particular feline enteric coronavirus (FECV), canine coronavirus (CCV), swine transmissible gastroenteritis coronavirus (TGEV), porcine respiratory coronavirus (PRCV) and human coronavirus (HCV), and which induces, in a host-dependent manner, a range of symptoms which range from mild enteritis to the severe debilitating disease, and, in some cases, up to death. In a particular aspect the present invention provides the use of MLCK inhibitors for the treatment of feline infectious peritonitis (FIP).

The invention relates to a CCV (canine corona virus) M protein monoclonal antibody and a preparation method thereof, and a preparation method of an immune colloidal gold test strip and particularly relates to a CCV M protein monoclonal antibody, comprising CCV M protein specific monoclonal antibodies 2H11 and 2G3. The preparation method of the CCV M protein monoclonal antibody comprises: preparinga CCV monoclonal antibody with separated and purified inactivated CCV as an immunogen by means of a monoclonal antibody technique; verifying the monoclonal antibody for the M protein by means of western-blot test, performing genetic cloning on the CCV M protein by means of recombinase ExnaseTMII to obtain a linear pCDNA3.1 vector, transfecting with 293T cells to carry out M protein expression, and carrying out IFA test verification to obtain two CCV M protein specific monoclonal antibodies 2H11 and 2G3. The invention also discloses the preparation method of the immune colloidal gold test strip. The CCV M protein monoclonal antibody herein has high specificity, high sensitivity, good operational simplicity and low cost, is applicable to clinical and field tests and is applicable to CCV etiological diagnosis, epidemiological investigation and quick diagnosis in animal hospitals.

The invention provides a group of nucleic acids for synchronously detecting and distinguishing five canine diarrhea viruses and also provides a multi-gene-chip high-flux molecular biological detectionmethod for the five canine diarrhea viruses. The nucleic acids include upper and lower primers and hybridization probes of a canine adenovirus type 1, a canine adenovirus type 2, canine coronaviruses, canine distemper viruses and canine parvoviruses of the five canine diarrhea viruses. By adopting the method, multi-gene-chip detection is performed by extracting the nucleic acids of the canine diarrhea viruses in samples to be detected, synchronous and accurate detection and distinguishing of the canine diarrhea viruses in samples to be detected are achieved, and the nucleic acids have the advantages of being good in specificity, high in sensitivity, good in stability, simple and convenient to operate and capable of achieving high-flux quick detection.

The present invention discloses a primer and a kit for detecting three common canine pathogen infections in dogs, and an application of the kit. The primer comprises three primer sets for canine picoronavis (CPV), canine coronavirus (CCV) and canine distemper virus (CDV), each primer set consists of 6 primers of a F3 primer, a B3 primer, a FIP primer, a BIP primer, a LF primer, and a LB primer, and sequences vary depending on the targeted pathogens. The detection kit comprises three detection reagents of CPV, CCV, and CDV, and also comprise a negative control, a pretreatment solution A and a pretreatment solution B. A use method of the kit is as follows: dipping patient dog samples (for detection of CPV and CCV, taking stool or anus swabs and for detection of CDV, taking eye or nose secretion), putting the samples into each detection reagent after treatment, conducting reaction at constant temperature of 65 DEG C, and determining negative and positive results according to color changesafter completion. Based on a LAMP technology, pathogen gene fragments are specifically amplified, sampling and detection can be completed within 60 minutes, and an easiest operation method is used torealize high-precision detection results.

The invention discloses a primer pair for detecting canine diarrhea viruses through a one-step method, a TaqMan probe, a method and application. Exploratory research on multiplex fluorescent PCR is carried out in multiple aspects of the specificity of a primer probe, a key factor in a reaction system, quality control construction and the like; a detection fragment is amplified by nucleic acid or reverse transcription products of a sample to be detected, and connected to a pMD18-T vector; plasmids are successfully constructed and used as a template; a multiplex real-time fluorescent quantitative PCR method of canine parvovirus, canine coronavirus, canine astrovirus and canine kobuviruses is established; the patent allows that at most four viruses causing canine diarrhea can be simultaneously detected in one reaction tube; the detection sensitivity is up to 1*10<2> copies; the detection sensitivity and the specificity of four canine diarrhea viruses can be obviously improved; furthermore, the detection period is effectively shortened; and thus, the patent has important meanings.

The invention discloses a multiplex PCR primer for detecting four viruses of a dog, a detection method and application. The primer comprises primers CCV-F and CCV-R for detecting a canine coronavirus,primers CPIV-F and CPIV-R for detecting a canine parainfluenza virus, and primers CAV-F and CAV-R for detecting a canine adenovirus type I and a canine adenovirus type II. The PCR detection method established by the primer can detect a single virus, can also simultaneously detect mixed infection of four viruses CCV, CPIV, CAV-1 and CAV-2, has the advantages of high detection sensitivity, good specificity and the like, can reduce the occurrence of false positive, can realize rapid, accurate, sensitive and early discovery of pathogen targets, provides technical support for establishing a standardized detection method for health monitoring of the dog, and can greatly save time and cost.

The invention discloses a canine distemper virus and canine coronavirus duplex PCR detection kit and a duplex PCR method for rapid identification and detection of canine distemper virus and canine coronavirus. According to the genome sequences of the canine distemper virus and canine coronavirus, a pair of PCR primers are designed respectively to amplify corresponding specific fragments and realize detection and identification of canine distemper and canine coronavirus. The invention utilizes the duplex PCR technology to conduct single tube synchronous detection of canine distemper virus and canine coronavirus, reduces the detection cost and workload of canine distemper virus and canine coronavirus, and realizes identification and detection of canine distemper virus and canine coronavirus. The invention establishes a duplex PCR method that strong specificity and high sensitivity, saves time and labor, and is easy to observe the result, and has the advantages of simple operation, strong specificity, high sensitivity and repeatability, and no complicated aftertreatment.

The present invention provides the amino acid and nucleotide sequences of a CCV spike gene, and compositions containing one or more fragments of the spike gene and encoded polypeptide for prophylaxis, diagnostic purposes and treatment of CCV infections.

The invention discloses a nanoRT-PCR detection method for identifying CCoVI and CCoVII. Through the optimization on the primers, annealing temperature and primer concentration in a nanoRT-PCR reactionsystem, the effective amplification of respective target strips of the CCoVI and the CCoVII can be obtained; meanwhile, the non-specific amplification between primers can be avoided. The lowest nucleic acid detection quantity of the nanoRT-PCR detection method is 100 times higher than that of an ordinary RT-PCR method; the products with the identical size with the expected segment size and the completely correct sequence are obtained through nanoRT-PCR amplification. The nanoRT-PCR method for identifying CCoVI and CCoVII provided by the invention has ultra-high sensitivity and specificity, belongs to an efficient detection measure, can be applied to the epidemic investigation of canine coronavirus diseases; meanwhile, the foundation can be laid for the identification and prevention and control of the CCoV; great practical significance and values are realized.

The invention relates to the technical field of molecular biomedicine, in particular to a PCR (Polymerase Chain Reaction) detection kit for cat and / or dog pathogens, a detection method and application. The kit is used for detecting canine distemper virus, canine influenza A virus, canine parainfluenza virus, canine parvovirus, canine coronavirus, canine rotavirus, canine babesia, canine ascaris, canine Ehrlichia, canine brucella, rabies virus, borrelia burgdorferi, reference gene ACTB, feline herpes virus, feline calicivirus and feline parvovirus. The kit comprises primers and probes of feline coronavirus, feline immunodeficiency virus, feline leukemia virus, feline mycoplasma, feline mycoplasma, feline chlamydia, giardia, toxoplasma, bartonella and reference gene GAPDH, collected DNA and RNA are added into the kit, a real-time fluorescence PCR instrument is adopted for PCR reaction, FAM, HEX, ROX and CY5 fluorescence signals are collected in each cycle, analysis of related pathogens is carried out, and the kit can be used for detecting the feline and the canine. Compared with a traditional detection method, the method has the advantages of higher specificity and higher sensitivity.

This invention relates to vaccines and methods for protecting dogs against disease caused by Bordetella bronchiseptica. This invention relates to vaccines and methods for protecting dogs against disease caused by Leptospira bratislava. This invention also relates to combination vaccines and methods for protecting dogs against disease or disorder caused by canine pathogens, for example, infectious tracheobronchitis caused by Bordetella bronchiseptica, canine distemper caused by canine distemper (CD) virus, infectious canine hepatitis (ICH) caused by canine adenovirus type 1 (CAV-1), respiratory disease caused by canine adenovirus type 2 (CAV-2), canine parainfluenza caused by canine parainfluenza (CPI) virus, enteritis caused by canine coronavirus (CCV) and canine parvovirus (CPV), and leptospirosis caused by Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae or Leptospira pomona.

The invention discloses a primer, a probe and a kit for detecting canine distemper virus of a giant panda. Based on the design of the primer and the probe, the canine distemper virus of the giant panda (giant panda / SX / 2014 strain) (Genbank:KP793921) is used as a template, the detection specificity is improved, and cross reaction between the canine distemper virus of the giant panda and other viruses such as canine parvovirus (CPV), canine coronavirus (CCV), canine parainfluenza virus (CPIV) and canine rotavirus (CRV) is avoided; when the primer and the probe are applied to detection of the canine distemper virus of the giant panda, the sensitivity is high, a detection limit is 5x101copy / microliters, and a detection result is reliable; and compared with an existing RT-PCR method, the primerand the probe are very high in conformity, and can realize portable quick diagnosis on site.

A canine respiratory coronavirus (CRCV) that is present in the respiratory tract of dogs with canine infectious respiratory disease and which has a low level of homology to the enteric canine coronavirus, but which has a high level of homology to all bovine coronavirus strains (e.g., Quebec and LY138) and human coronavirus strain OC43.

The invention discloses an RDA method and kit for rapidly detecting canine coronaviruses (CCV). The kit comprises a specific primer pair and an RDA fluorescence labeling probe, so as to realize safe,specific, sensitive, simple and convenient detection of the canine coronaviruses (CCV) and overcome the defects of the existing traditional detection technology. According to the kit provided by the invention, a nucleic acid extraction step can be omitted, the detection of the canine coronaviruses (CCV) is realized under a constant temperature condition within 20 minutes, and the specificity is 100%. Compared with a common PCR method, the RDA fluorescence method has the advantages of reaction at constant temperature, no need of temperature change and complex instruments, shorter reaction timeand suitability for on-site rapid detection. The method and the kit provided by the invention have the characteristics of simple and rapid operation, good specificity, high sensitivity, low cost and the like, can provide an effective technical means for on-site rapid detection and screening of the canine coronaviruses (CCV), and have a wide application prospect.

The invention relates generally to the field of virology, and relates to the identification and characterization of the targets involved in the evasion strategy of viruses and to the use thereof in methods to identify anti-viral compounds. More in particular to identify compounds which are modulators of myosin light chain kinase (MLCK), a target within said entry and immune-evasion strategy. Other aspects of the invention are directed to anti-viral compounds identified using the models and methods of the present invention, as well as to the use thereof in treating viral infections, such as for example caused by the feline infectious peritonitis virus (FIPV), a coronavirus which belongs to an antigenic group which comprises in particular feline enteric coronavirus (FECV), canine coronavirus (CCV), swine transmissible gastroenteritis coronavirus (TGEV), porcine respiratory coronavirus (PRCV) and human coronavirus (HCV), and which induces, in a host-dependent manner, a range of symptoms which range from mild enteritis to the severe debilitating disease, and, in some cases, up to death. In a particular aspect the present invention provides the use of MLCK inhibitors for the treatment of feline infectious peritonitis (FIP).

The invention discloses a nucleic acid composition for detecting canine coronavirus and canine parvovirus, a kit and application, and relates to the technical field of pathogen detection. The composition comprises a first nucleic acid composition for detecting canine coronavirus and a second nucleic acid composition for detecting canine parvovirus, the composition can be used for real-time fluorescent quantitative PCR detection of canine digestive tract virus, and the inventor verifies that the nucleic acid composition is good in pathogen detection specificity and high in sensitivity. The lowest detection limits of the two pathogens reach 5 * 10 < 2 > copies / mL, and early diagnosis or treatment of affected dogs is facilitated. Corresponding detection kits and detection reagents can be prepared based on the nucleic acid composition.

The invention discloses a leptospira canicola, leptospira icterohaemorrhagiae and canine coronavirus triple inactivated vaccine. The leptospira canicola, leptospira icterohaemorrhagiae and canine coronavirus triple inactivated vaccine is prepared by inactivating leptospira canicola, leptospira icterohaemorrhagiae and canine coronavirus epidemic strains separated in China and then adding an adjuvant. The invention relates to a method for isolated culture, identification and inactivation of pathogens, a production process, vaccine preservation and an immunization method. The canine triple inactivated vaccine is used for preventing leptospira canicola, leptospira icterohaemorrhagiae and canine coronavirus diseases, high antibodies can be generated after immunizing dogs, and the triple inactivated vaccine has a good protective effect on virulent attack. Leptospira is a human and animal infectious disease, dogs are human companion animals, and pet dogs are in close contact with human beings, so that the triple inactivated vaccine has good social benefits and important public health significance.

The invention discloses a preparation method of a canine coronavirus chemiluminescence detection reagent, and belongs to the technical field of biological detection preparations. The method comprises the following steps: preparing a magnetic microspheron-G19 conjugate: activating magnetic microspheres by adopting EDC and NHS, adding a canine coronavirus antibody G19, oscillating and coupling the material, and storing the material in a magnetic microsphere buffer solution for later use; preparing a G15-acridinium ester conjugate: fully and uniformly mixing G15 and acridinium ester, carrying out labeling coupling, carrying out oscillation labeling, sealing, purifying, and storing in a luminous reagent buffer solution for later use. The method is used to obtain the luminosity RLU of the detected sample. According to the preparation and use method of the chemiluminescence detection reagent, the blank of domestic canine coronavirus chemiluminescence detection reagents is filled, and qualitative, quantitative and rapid detection of canine coronavirus antigens is truly realized.

The invention provides a bigeminy fluorescent quantitative detection reagent for canine coronavirus, and relates to the technical field of virus detection. The canine coronavirus bigeminy fluorescent quantitative detection reagent comprises a CPV / CCV bigeminy detection reagent card and a sample diluent, the CPV / CCV bigeminy detection reagent card comprises a reagent card body, and a sample adding hole, a combination pad and an NC membrane which are sequentially arranged on the reagent card body, the combination pad contains fluorescent microspheres labeled by fluorescence, and the fluorescent microspheres comprise microspheres coupled with a canine coronavirus antibody and microspheres coupled with a canine parvovirus antibody. The concentration of canine parvovirus / canine coronavirus in canine faeces is detected by adopting a double-antibody sandwich method, a test sample is added into a diluent to be uniformly mixed and dropwise added into a sample adding hole, and an antigen can be diffused onto a combination pad along with the sample diluent and is combined with a fluorescently-labeled antibody, so that simultaneous detection of canine coronavirus and parvovirus is realized; detection is accurate and the effect is good.

Problem to be Solved: The present invention provides a novel mucosal adjuvant.Solution: The present invention provides a mucosal adjuvant composition containing at least a composition comprising molecules having a molecular weight in a range of 100 to 300 kDa obtained from cells or culture fluid of Bordetella bronchiseptica. Administration of the mucosal adjuvant composition to a non-human animal at a surface of the mucous membrane can enhance immunity at the surface of mucous membrane. Therefore preventive effects against trans-mucosal infection can be increased by administering an inactivated vaccine against trans-mucosal infection and the mucosal adjuvant composition to a non-human animal at a surface of mucous membrane. The present invention is effective for preventing trans-mucosal infections, including one or more infections of e.g., canine parainfluenza, canine adenovirus, canine coronavirus, canine parvovirus, canine distemper virus, canine herpesvirus, reovirus and pneumovirus.