38 results about "Feline parvovirus" patented technology

The invention discloses an application of a compound pulchinenoside B4 shown as a formula I or pharmaceutically acceptable salt thereof in the preparation of drugs for treating viral and / or bacterialdiseases. The compound pulchinenoside B4 has strong biological activity, and has excellent treating effects on endometritis, foot rot, feline parvovirus, canine parvovirus, canine distemper, canine renal failure and canine acute nephritis.

The invention provides a recombinant feline parvovirus VP2 protein antigen and application thereof in vaccine preparation and virus diagnosis. A plurality of B cell antigens of the feline parvovirus VP2 protein are subjected to tandem expression by using a prokaryotic expression vector. The expressed recombinant protein is purified and used as a coating antigen for detecting the feline parvovirus antibody. And compared with a whole virus coating method in parallel, the values of detected positive and negative serum are highly consistent. The antigen treatment method provided by the is convenient, the test time is shortened, and the operation steps are simpler. According to the invention, an indirect ELISA method is established for detecting the antibody level of the feline parvovirus in feline serum, which has the characteristics of good repeatability and high specificity, and can be used for feline parvovirus serology investigation. Therefore, the indirect ELISA detection kit for the feline parvovirus based on the tandem expression of the VP2 protein B cell antigens, provided by the invention, is very suitable for the detection of clinical large samples and is suitable for large-scale popularization.

The invention provides a feline panleukopenia virus antibody ELISA kit and a detection method for a feline panleukopenia virus antibody ELISA. The feline panleukopenia virus antibody ELISA kit comprises an enzyme-labeled plate and an enzyme-labeled antibody which are coated with antigens; the antigens are FPV purified antigens, and the enzyme-labeled antibody is a goat anti-feline IgG antibody labeled by horse radish peroxidase; and the feline panleukopenia virus antibody ELISA kit and the detection method for the feline panleukopenia virus antibody ELISA have very high sensibility, specificity and accuracy for the detection of the feline panleukopenia virus antibody.

The invention belongs to the technical field of vaccine preparation, relates to a novel feline parvovirus vaccine, and discloses a coding gene of a feline parvovirus VP2 protein. According to the invention, an FPV VP2 protein sequence is coded and optimized through a genetic engineering technology, a recombinant plasmid is constructed, a recombinant protein is expressed by using escherichia coli, and the FPV VP2 protein is co-expressed with four molecular chaperones respectively; and a target protein is purified by different purification methods to obtain a virus-like particle which is similar to natural viruses in size and has good hemagglutination. A new thought and a new basis are provided for novel feline parvovirus vaccines.

The invention relates to the technical field of molecular biomedicine, in particular to a PCR (Polymerase Chain Reaction) detection kit for cat and / or dog pathogens, a detection method and application. The kit is used for detecting canine distemper virus, canine influenza A virus, canine parainfluenza virus, canine parvovirus, canine coronavirus, canine rotavirus, canine babesia, canine ascaris, canine Ehrlichia, canine brucella, rabies virus, borrelia burgdorferi, reference gene ACTB, feline herpes virus, feline calicivirus and feline parvovirus. The kit comprises primers and probes of feline coronavirus, feline immunodeficiency virus, feline leukemia virus, feline mycoplasma, feline mycoplasma, feline chlamydia, giardia, toxoplasma, bartonella and reference gene GAPDH, collected DNA and RNA are added into the kit, a real-time fluorescence PCR instrument is adopted for PCR reaction, FAM, HEX, ROX and CY5 fluorescence signals are collected in each cycle, analysis of related pathogens is carried out, and the kit can be used for detecting the feline and the canine. Compared with a traditional detection method, the method has the advantages of higher specificity and higher sensitivity.

The invention discloses an anti-FPV (feline parvovirus) feline genetic engineering antibody ELISA kit and a detection method thereof and belongs to the technical field of genetic engineering antibodyanalysis and detection. The kit comprises an ELISA plate coated by an antigen and an enzyme-labeled antibody, wherein the antigen is a FPV-VLPS purified antigen, and the enzyme-labeled antibody is a goat anti-cat IgG antibody labeled by horseradish peroxidase. The detection method comprises the following steps: coating the ELISA plate; drawing a standard curve; adding the goat anti-cat IgG antibody labeled by the horseradish peroxidase, carrying out TMB color developing and stop of a stop solution; measuring an OD450 value of each reaction hole; and performing judgment according to judgment standard and the like. The kit and the detection method have very high sensitivity, specificity and stability in detecting anti-FPV (feline parvovirus) feline genetic engineering antibodies, and providestrong support for study in detecting the level of the anti-FPV (feline parvovirus) feline genetic engineering antibodies in animals before and after emergency immunization.

The invention belongs to the technical field of virus antibodies, and discloses a feline panleukopenia virus antibody sequence, a tetrapeptide chain molecule, an immunoglobulin molecule and application, wherein the feline panleukopenia virus antibody sequence is a heavy chain variable region amino acid sequence SEQ ID NO: 1 and a nucleotide sequence SEQ ID NO: 3; and a light chain variable regionamino acid sequence SEQ ID NO: 2 and a nucleotide sequence SEQ ID NO: 4. A sequence screening method comprises the following steps of preparing a bacteriophage antibody; screening a bacteriophage antibody library; identifying an anti-feline panleukopenia virus feline-derived bacteriophage single-chain antibody by using a PhageELISA method; sending a scFv bacterial liquid with a positive PhageELISAidentification result to a sequencing company for sequencing to obtain variable region sequences of a heavy chain and a light chain of the feline-derived anti-feline panleukopenia virus genetic engineering antibody. The invention provides support for the construction of feline-derived anti-feline panleukopenia virus genetically engineered antibodies with high affinity and low immunogenicity. Themethod has important significance for promoting the development of feline-derived antibody drugs.

The invention provides the feline parvovirus VP2 protein and the prepared virus-like particles, wherein the amino acid sequence of the VP2 protein is SEQ ID NO:2; the nucleotide sequence of its coding gene is SEQ ID NO:1. Another aspect of the present invention provides a recombinant baculovirus, which contains the nucleotide fragment encoding the above-mentioned VP2 protein; the recombinant baculovirus constructed in the present invention is used to prepare virus-like particles of feline parvovirus in insect cells. The invention also provides a feline parvovirus subunit vaccine, which includes an antigen and a vaccine adjuvant, and the antigen used is the virus-like particle prepared by the invention. The vaccine prepared by the invention can increase the antibody titer of the cat after immunizing the cat, and can effectively prevent the infection of the feline parvovirus.

The invention belongs to the technical field of biology, and particularly relates to a primer probe set and a kit for detecting FPV through an RAA fluorescence method.The primer probe set can detect more FPV genotypes after degeneracy design, compared with other primer probe sets, it is found that the primer probe set has the advantages of being high in sensitivity and better in detection effect, and meanwhile the primer probe set can be used for detecting the FPV genotypes. The invention also prepares a corresponding RAA kit and establishes a corresponding RAA detection method, tests show that the kit can be carried out under a constant temperature condition (37-42 DEG C), can realize rapid and qualitative detection of the feline parvovirus within 5-30 min, has the characteristics of convenience in operation, strong specificity, high sensitivity and good repeatability, and can completely meet the requirements of rapid diagnosis of the feline parvovirus, rapid diagnosis of the feline parvovirus, rapid diagnosis of the feline parvovirus, rapid diagnosis of the feline parvovirus, rapid diagnosis of the feline parvovirus, rapid diagnosis of the feline parvovirus and rapid diagnosis of the feline parvovirus. The kit is suitable for clinical rapid detection and has a wide application prospect.

The invention discloses a primer set, a reagent and a method thereof for detecting feline parvovirus based on a polymerase helical reaction. The primer set includes specific primers PSR-FPVF and PSR-FPVR, accelerated primers PSR-FPVF FJ and PSR-FPVF RJ, the nucleotide sequence of the specific primer PSR-FPVF is shown in SEQ ID NO.1, the nucleotide sequence of the specific primer PSR-FPVR is shown in SEQ ID NO.2, and the nuclear sequence of the accelerated primer PSR-FPVF FJ The nucleotide sequence is shown in SEQ ID NO.3, and the nucleotide sequence of the accelerating primer PSR‑FPVF RJ is shown in SEQ ID NO.4. The PSR detection method constructed by this primer set is simple to operate, has low requirements for detection equipment, and greatly shortens the detection time. It can be completed within 45 minutes at a constant temperature of 67°C. After the amplification, a final concentration of 20×SYBR is added to the system. Green I nucleic acid dye, the detection result can be judged by naked eyes under visible light, which greatly improves the detection efficiency.

The invention discloses a cat parvovirus neutralizing monoclonal antibody and application thereof, and relates to the technical field of bioengineering. The invention provides a hybridoma cell strain which is preserved in China Center for Type Culture Collection on May 6, 2022, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: C2022111. The invention also provides a cat parvovirus neutralizing monoclonal antibody which is secreted by the hybridoma cell, and the specific sequences of the cat parvovirus neutralizing monoclonal antibody are shown as SEQ ID NO: 1 and SEQ ID NO: 2. The hybridoma cell can secrete and generate the cat parvovirus neutralizing monoclonal antibody, and the monoclonal antibody is proved to have very high neutralizing activity through in-vitro cellular level neutralization tests and animal protective experiments, can specifically treat FPV, is simple to prepare and convenient to operate, and has great significance for preventing and treating the FPV.

The present invention relates to a vaccine for immunizing a cat against feline viruses. The present invention also relates to a nucleic acid clone that encodes the capsid protein of the isolated feline calicivirus. The present invention further relates to a live or killed vaccine comprising the isolated feline calicivirus, a subunit vaccine comprising the capsid protein of the isolated feline calicivirus, a nucleic acid vaccine comprising a nucleic acid clone of the isolated feline calicivirus, and a recombinant virus vector vaccine comprising nucleic acid encoding the capsid protein of the isolated feline calicivirus. The present invention also relates to a method for identifying a feline calicivirus useful for producing a vaccine composition and for assays for diagnosing cats infected with feline calicivirus. Also disclosed is a method of immunizing animals, especially cats, against disease, in particular against feline calicivirus (FCV). The method includes administering to a cat therapeutically effective amounts of first and second FCV vaccines. The first vaccine is administered orally or parenterally (e.g., subcutaneously, intramuscularly, and the like). The second vaccine is administered orally or oronasally N days following administration of the first vaccine, wherein N is an integer from 3 to 120, inclusive. A third vaccine administration may also be given. The present invention also describes methods and materials for treating and immunizing animals with vaccine, and in particular cats against both FPV or Feline Parvovirus, which has also been called Panleukopenia or FPL and against another disease, FHV or Feline Herpes Virus, which has also been called Feline Rhinotracheitis Virus.