The invention discloses compound traditional Chinese medicine perfusion liquor for preventing and treating the endometritis of a dairy cow, which can be used for preventing and treating the diseases of the dairy cow, such as the endometritis and the like caused by bacteria, fungi and infusorium and belongs to the field of animal medicines. The invention also discloses a preparation method of the compound traditional Chinese medicine perfusion liquor, and the method has simple preparation process. The compound traditional Chinese medicine perfusion liquor comprises the following medicinal components of cnidium fruit, kuhseng, coptis root and licorice, and the compound traditional Chinese medicine perfusion liquor has obvious curative effect and fast effect taking in the diseases of the dairy cow, such as the endometritis and the like caused by the bacteria, the fungi and the infusorium and can effectively prevent and treat the uterine diseases of the dairy cow, such as postpartum infection and the like caused by the bacteria, the fungi and the infusorium. The compound traditional Chinese medicine perfusion liquor has stable quality, convenient use and low price, also has the advantages of no toxic or side effect, absence of antibiotics and the like and can effectively ensure the safety of a milk product.