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Lysostaphin freeze dried powder used for preventing and treating cattle edometritis

A technology of lysostaphin and freeze-dried powder, which is applied in the direction of anti-inflammatory agent, freeze-dried delivery, powder delivery, etc., can solve the problems of poor clinical effect, unfavorable breeding of dairy cows, endometrial fibrosis, etc., and achieve Promote the repair of uterine mucosa, strong bactericidal effect, strong bactericidal effect

Active Publication Date: 2007-02-14
昆山博青生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such as CN1302634A (medicine for treating uterine inflammation), but because the drug effect of traditional Chinese medicine is slow, so that the disease is protracted, or recurrent, so the clinical effect is not good
[0005] US 4692452 (Method for treatment of endometritis in mammalian females), reported the use of chemical agents to treat endometritis in animals (especially cows), but chemical agents are often irritating to the endometrium and can cause endometrial fibrosis , so that scars are formed, which is not conducive to further breeding and conception of dairy cows

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of embodiment 1 lysostaphin freeze-dried powder

[0037] 1. Composition and ratio

[0038] Lysostaphin 0.4%; Bovine serum albumin 10%; Glycine 30%; Mannitol 10%; Potassium dihydrogen phosphate 15%; Disodium hydrogen phosphate 30%.

[0039] 2. Preparation of semi-finished products

[0040] Weigh solid KH 2 PO 4 and Na 2 HPO 4 .12H 2 O, prepare 0.2mol / L phosphate buffer A (pH value 6.5 phosphate buffer, concentration is 0.2M). According to the formulation prescription, weigh the required glycine and mannitol, measure the required volume of bovine serum albumin, add it to the part A solution, stir and dissolve, and obtain the solution B. Measure the stock solution of recombinant lysostaphin, add it to solution B, make the biological activity of recombinant lysostaphin in the final solution be 400U / ml, add A solution to the final volume, and what is obtained at this time is the recombinant lysostaphin Semi-finished solution.

[0041]...

Embodiment 2

[0047] Example 2 Rabbit Subcutaneous Injection Topical Recombinant Lysostaphin Repeated Administration Toxicity Test

[0048] 1. Purpose of the experiment

[0049] The purpose of this experiment is to observe the nature, degree, development and recovery of toxic reactions in rabbits through subcutaneous injection of recombinant lysostaphin for external use, so as to provide reference materials for clinical safety drug dosage design and clinical toxicity monitoring.

[0050] 2. Experimental method

[0051] 24 New Zealand white rabbits, weighing 2.5-3.0kg. There are 3 dosage groups for recombinant lysostaphin for external use, and the dosages are 100, 20 and 4mg.kg respectively. -1 (equivalent to 241936, 48387 and 9677U·m -2 ), the solvent control group (0.9% sodium chloride injection), the subcutaneous injection amount is 1mg.kg -1 . Administered continuously 7 days a week for a total of 4 weeks.

[0052] 3. Experimental results

[0053] After administration, the...

Embodiment 3

[0056] Embodiment 3 Lysostaphin freeze-dried powder stability test

[0057] 1. Test equipment

[0058] Test strain: Staphylococcus aureus (ATCC6538);

[0059] Neutralizer: 0.5% sodium thiosulfate, 2% Tween-80, 0.5% lecithin in PBS;

[0060] Phosphate buffer solution (PBS0.03mol / l pH7.2).

[0061] 2. Test method

[0062] The 24h slant culture of the test bacteria was washed with PBS to make a bacterial suspension.

[0063] Take 4 pieces each of the sample piece (2.0cm×3.0cm) and the control piece (the same material as the sample, the same size, but without antibacterial material, and sterilized), divide them into 4 groups and place them in 4 sterilized in the bacterial plate.

[0064] Take the above-mentioned bacterial suspension, add 100 μL dropwise on each test piece and control sample, spread evenly, start timing, and act for 2, 5, 10, 20 minutes, use sterile tweezers to drop the sample into the corresponding medium containing 5mL respectively. In the t...

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PUM

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Abstract

A freeze-dried powder-injection of staphylococcus lysozyme for preventing and treating the endometritis of cow and the skin mucosa infection of other animals, and cleaning and restoring the postpartum uterus of cow is composed of staphylococcus lysozyme, bovine serum albumin, glycine, mannitol, and phosphate. Its preparing process is also disclosed.

Description

technical field [0001] The invention relates to freeze-dried biological preparations, in particular to lysostaphin freeze-dried powder Background technique [0002] Endometritis is one of the common diseases of dairy cows. Postpartum endometritis in dairy cows can prolong the time of uterine involution and disturb the estrous cycle. Inflammatory exudate can change the pH of the uterine environment, which is not conducive to the survival of sperm and eggs, reduces the conception rate, and leads to infertility. At the same time, it can also stimulate the receptors on the inner wall of the uterus for a long time, and through reflexes, it can cause hormone imbalance and cause ovarian diseases. . Also can secondary mastitis, laminitis etc. in addition. [0003] The incidence of endometritis in dairy cows is between 15-20%, most of which occur postpartum. In the United Kingdom, 95% of cow infertility is caused by endometritis, while the loss caused by infertility in the United ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/43A61K9/19A61P15/00A61P29/00
Inventor 黄青山陆婉英
Owner 昆山博青生物科技有限公司
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