2270 results about "Lysozyme" patented technology

The invention relates to a compound biological antiseptic and preservation agent for food. The compound biological antiseptic and preservation agent is prepared by evenly mixing pure biological solid preparations or products such as epsilon-polylysine, nisin, natamycin, lysozyme, bacillus subtilis, ascorbic acid, a toona sinensis leaf extract, a water-soluble resveratrol preparation, tea polyphenol and chitosan. The compound biological antiseptic and preservation agent has a strong inhibition effect on spoilage bacteria and pathogenic bacteria in various foods and good inhibition action on oxidative deterioration of the food. Compared with the existing various preservatives, the compound biological antiseptic and preservation agent has the characteristics of broad antibacterial spectrum, high safety, high efficiency and convenience in use.

The invention relates to a foodstuff that can enhance the milk yield of lactation period sow, consisting of the following components in weight portion: 250 to 350 portions of calcium hydrogen phosphate, 250 to 350 portions of mountain meal, 100 to 130 portions of common salt, 30 to 40 portions t of choline, 45 to 55 portions of complex trace elements, 8 to 12 portions of complex vitamin, 30 to 50 portions of potassium chloride, 5 to 15 portions of methionine, 15 to 35 portions of lysine, 2 to 8 portions of valine, 50-150 portions of zeolite powder, 15 to 25 portions of medium and short chain fatty acids, 5 to 20 portions of feed attractant, 0.5 to 1 portions of lysozyme, 10 to 25 portions of mycotoxin absorbent, 5 to 7 portions of vitamin E, 2 to 5 portions of phytase, and 3 to 6 portions of chromium Picolinate. The invention adds nutritive additives and mycotoxin absorbent etc. into the foodstuff, thereby obviously intensifying the immunity of lactation period sow, dramatically enhancing the milk yield, bringing forward the oestrous period, and significantly increasing the ablactation weight of the piglet.

The present invention is directed to nanoparticulate active agent compositions comprising lysozyme as a surface stabilizer. Also encompassed by the invention are pharmaceutical compositions comprising a nanoparticulate active agent composition of the invention and methods of making and using such nanoparticulate and pharmaceutical compositions.

The invention pertains to the field of microbial feed additives and relates to a novel feed additive containing lysozyme, probiotic powder and a carrier and a preparation method thereof, wherein the probiotic powder is bacillus subtillis powder and / or lactobacillus sp. powder and / or saccharomyces cerevisiae powder. The novel feed additive is the first time to add the lysozyme to a probiotic preparation which is then prepared to the novel feed additive. A product has and enhances the advantages of both the microecological preparation and the lysozyme, has better effects than the independently added microecological preparation or the lysozyme and can significantly improve weight increment and the feed conversion rate, the balance of intestinal flora as well as immunity and disease resistance. The novel feed additive has multi-effects of treatment, prevention, health care and immunity strengthening as well as simple preparation technology and is applicable to industrialized application.

The invention provides a feed for improving the disease resistance of a grass carp. The feed is characterized by comprising soybean cake, wheat meal, shell meal, fish meal, table salt, rice bran, fish oil, betaine, traditional Chinese medicine feed premix additive, zinc-containing polypeptide, zinc-containing microelement additive, vitamin mixture additive at least containing vitamin C, vitamin E and inositol, yeast and amino acid mixture additive at least containing threonine. The feed provided by the invention can improve the immunity of the organism of the grass carp and protect the grass carp from getting sick; the application of antibacterial chemical medicines and anti-virus chemical medicines is reduced to a certain degree, the survival rate of the grass carp is increased, and the activity of lysozyme and superoxide dismutase in the grass carp is improved; and moreover, the feed ensures little drug residue and low toxicity, and does not influence the quality of the grass carp or harm the human health.

The invention provides a method for preparing lysozyme molecular imprinting nano particles with magnetic responsiveness and extremely high adsorption capacity. In the method, lysozymes are used as template molecules. The method comprises the following steps of: carrying out template polymerization under the conditions that suitable functional monomers, a cross-linking agent and an initiator and magnetic nano particles the surfaces which are coated with SiO2 and are modified by sulfydryl and acryloyloxy exist; and washing out the template molecules to obtain the lysozyme molecular imprinting nano particles with magnetic responsiveness and high adsorption capacity. The invention also provides a method for preparing the nano particles. The lysozyme molecular imprinting nano particles prepared by adopting the method disclosed by the invention have the characteristics of high adsorption capacity, simpleness for preparation, low cost, strong specific absorption, high physical, chemical stability, capability of being recycled repeatedly and the like.

The invention relates to gum-protecting whitening toothpaste. The gum-protecting whitening toothpaste is prepared from the following components: sorbitol, xylitol, sodium phytate, brilliant blue pigment, sodium lauroyl sarcosine, ethylene diamine tetraacetic acid (EDTA) disodium, plant chitosan, trehalose, sodium saccharin, sodium pyrophosphate, glycerol, sodium hyaluronate, xanthan gum, hydroxyethyl cellulose, polyvinyl pyrrolidone (PVP) cross-linking agent, shell powder, silicon dioxide (friction type), silicon dioxide (thickening type), zinc citrate, lysozyme, pawpaw extract, vitamin E, peppermint essence, silica (whitening particles) and pure water. The components coordinate with one another, so that the effects of protecting gum and whitening teeth are achieved.

The invention relates to a method for preparing a novel ocean lysozyme antibiotic preservative coated film. The method is characterized by comprising the following steps of: preparing solution of sodium alginate by dissolving sodium alginate and glycerol in sterile water; adding a compound preservative formed by cean lysozyme, potassium sorbate and tea polyphenol in the solution of sodium alginate to prepare solution of antibiotic film; and colloidizing the solution of antibiotic film in 4 to 6 weight percent CaC12 solution to obtain the film. The method has the advantages of simple process, low cost, high performance-to-price ratio, good preservative effect, safety and wide application to freshness keeping of various aquatic products.

The invention relates to a bacillus S-12 of high yield ocean microorganism lysozyme which separated from sea mud of The East China Sea.It is conserved at the type cultivating matter protect center of HuHan China, with CCTCC NO:M204089.The ratio that this S-12 and Bacillus bacterial homology is between 98% and 99%.It is a new fungus ilk with a filiation.The bacteriolysin molecular weight produced by this fungus is 39KD,consisted of 354 aminophenolls. It can be widely used in cosmetic, medicament, disinfector and breeding fields.

The invention belongs to the technical field of bioremediation of marine oil pollution, and relates to an immobilized algal-bacterial symbiotic system for processing petroleum pollutants. The immobilized algal-bacterial symbiotic system is prepared by the following steps: (1) selection and axenic cultivation of algae species, firstly carrying out lysozyme and sodium dodecyl sulfate (SDS) sterile pretreatment on chlorella algae fluid, and then inoculating into a BG11 culture solution containing antibiotics to carry out sterile cultivation, so as to obtain sterile chlorella algae fluid for later use; (2) selection and cultivation of petroleum degrading bacteria; (3) preparation of composite carrier bacteria algae gel balls, namely immobilizing the sterile chlorella algae fluid obtained in the step (1) and the petroleum degrading bacteria obtained in the step (2) by adopting sodium alga acid and polyvinyl acetate (PVA) as immobilized carriers, and finally obtaining the immobilized algal-bacterial symbiotic system. The immobilized algal-bacterial symbiotic system can float on the water to carry out degradation of petroleum pollution, and is free of secondary pollution, free of toxic action on microorganisms, rapid in reaction speed, stable and reliable in operation, less in microorganism loss, and free of separation.

A natural anti-staling agent and filming agent for crystal pork is proportionally prepared from nisin, thia-phenol, lysozyme and filming agent chosen from glacic acetic acid- chitosan mixture and callegen or pollutant polyose.

The invention relates to the fields of microorganism preparations and aquaculture, and discloses a lysozyme microcapsule. The microbial lysozyme microcapsule is prepared by the following steps of: (1) dropping a mixing solution of sodium alginate and lysozyme into an acetic acid solution containing chitosan and calcium chloride, and stirring; (2) after dropping, curing for 0.5-4hours, and taking and washing a solid to obtain lysozyme / sodium alginate / chitosan beads; and (3) pre-freezing the lysozyme / sodium alginate / chitosan beads, and freeze-drying. The microcapsule disclosed by the invention is used for preparing fish feed, and is capable of reserving comparatively high activity of the microbial lysozyme after being extruded at the high temperature in the feed processing; in the process of conserving at normal temperature, the stability of the microbial lysozyme can be kept for more than half an year; and in feeding and breeding, the microbial lysozyme can bear the strong acid and digesting environment in the stomach of the aquatic animal, release in the intestinal tract and develop an effective growth-promoting healthcare effect.

The invention provides for cells containing nucleic acids which include lysozyme gene expression controlling region nucleotide sequences which typically are linked to a polynucleotide encoding a heterologous polypeptide.

A process for spirulina deodorization relating to a kind of spirulina, particularly, a method of deodoeization by means of fermentation. And an effective method for spirulina deodorization is provided. Steps are, spirulina wall-breaking; adding glucose into already wall-broken spirulina liquid, inoculating active dried yeast, fermenting. Said spirulina wall-breaking by means of enzymolysis-isotropic wall-breaking and its steps are, adopting lysozyme enzymolysis, after enzymolysis to be crushed evenly. In the best conditions of technique, said wall-broken spirulina liquid containing slight fresh flavor and keeping original color.

The invention relates to a compound rice cake antistaling agent and a fresh keeping method. The compound antistaling agent comprises 0.03-0.1wt% of nisin, 0-0.1wt% of lysozyme, 0.03-0.1wt% of natamycin and a film forming agent; and the film forming agent comprises chitosan, acid and water, the specific proportions are 1-3wt% of the chitosan, 0.5-1wt% of the acid and the water, and a pH value of the film forming agent is controlled at 3.0-5.0, wherein, the acid is selected from lactic acid, citric acid or glacial acetic acid. The rice cake is soaked into 3-4 folds of the compound antistaling agent by volume for 1-5minutes, and then the rice cake is packaged in vacuum. The fresh keeping method can help better preserve the quality of the rice cake, inhibit external microbe invasion, sterilize the rice cake inside, and effectively solve the problem of relatively hard texture of the rice cake.

The invention relates to biological complex enzyme toothpaste and a preparation method thereof. The toothpaste is prepared from the following components by weight percentage: 5-10% of sorbierite, 15-20% of hydrous silica, 10-20% of aluminum hydroxide, 10-20% of glycerin, 2-3% of sodium lauroyl sarcosine, 1-1.3% of flavor, 0.8-1.2% of cellulose gum, 0.3-0.6% of carrageenin, 0.3-0.5% of droxyethylcellulose, 0.5-1.2% of sodium phytate, 0.15-0.3% of saccharin sodium, 0.3-0.5% of strontium chloride, 0.1-0.3% of trisodium phosphate, 0.00003-0.00008% of staphylococcus lysozyme, 0.00005-0.00015% of muramidase, 0.00001-0.00004% of proteolytic enzyme, 0.00002-0.00005% of glycosidic hydrolase, 0.00002-0.00005% of peroxydase, 0.08-0.15% of borneol, 0.1-0.15% of borax, 0.08-0.1% of vermilion, 0.1-0.15% of natrii sulfas exsiccatus, 0.05-0.1% of triclosan, 0.1-0.4% of allantoin, and the balance of water. The total weight percentage of the components is 100%. The biological complex enzyme toothpaste has complete function.

The invention relates to a biogenic composite pork preservative. The biogenic composite pork preservative comprises 4.0-4.8% of a bacterial depressant, 10-14% of a synergist, 27-33% of Chinese herbal medicine extract A, 16-20% of Chinese herbal medicine extract B and the balance water. The bacterial depressant contains nisin, natamycin, lysozyme, chitosan and propolis supercritical CO2 extract. The synergist contains acetic acid, vitamin C, vitamin E and sodium lactate. The Chinese herbal medicine extract A is prepared through extracting grape seeds, garlic, clove, cassia bark, rosemary, pepper and ginger through an ethanol extraction method and then blending the extract products, Origanum vulgare L. essential oil and tea crude extract. The Chinese herbal medicine extract B is prepared through water extraction of konjak, Chinese honeylocust fruits, rheum officinale and pomegranate peel. The biogenic composite pork preservative has a reasonable formula, is safe for eating, can be prepared by simple processes, is convenient for operation in use and has good fresh-keeping effects.

A method for simultaneously extracting the macrogenomic DNA and total RNA of microbe from the sea sediment includes such steps as treating the sediment by modifying solution, adding sand, oscillating, adding proteinase and lysozyme, cracking adding SDS and PVPP solution, cracking further, adding extrant, extracting, depositing in isopropanol and purifying by nucleic acid adsortion resin.

The invention discloses a preparation method of a controlled release antibacterial film. The method includes the following steps of: (1) dissolving zein and a hydrophobic antibacterial agent into an alcoholic solution; (2) preparing a sodium caseinate aqueous solution; (3) adding the sodium caseinate aqueous solution into the solution obtained in step (1) under stirring; (4) subjecting the solution obtained in step (3) to rotary evaporation and centrifugation, then freeze drying the supernatant, thus obtaining nanoparticle powder; (5) dissolving a film-forming matrix in water, adding the nanoparticle powder and lysozyme to form a mixed solution; and (6) adding glycerin, and conducting film pouring, thus obtaining the film product. The method provided in the invention can simultaneously reduce initial microbial activity and inhibit microbial growth for a long term, and has the advantages of low instrument requirement, and controllable bacterium inhibiting intensity, thus having broad application prospects in food and medical products.

The invention discloses a composition for preparing a camel milk facial mask, and particularly a composition for preparing a camel milk moisturizing skin-calming facial mask, a camel milk moisturizing clear facial mask, a camel milk moisturizing compact facial mask and a camel milk moisturizing conditioning facial mask according to functions. By virtue of the synergism of camel milk, water, sodium carbomer, glycerinum, xanthan gum, butanediol, sodium hyaluronate, poly(sodium L-glutamate), skin conditioner and preservative, the characteristics and advantages of the camel milk are maximally maintained, so that the composition for preparing the cameral milk facial mask has the advantages of the camel milk, namely, proteins, vitamins, minerals and fatty acid rich in the composition can provide rich nutrient substances for the skin and can improve the face skin; particularly, the camel milk contains lactoferrin and lysozyme which are short in cow milk, so that the composition has the effects of sterilizing, killing demodex, removing toxicity, softening stratum corneum epidermidis, moisturizing and tendering the skin, preventing wrinkles and aging resistance, and delaying senescence.

The invention discloses a compound biological antistaling agent for pork, which is prepared by the following components according to the mass proportions of: 0.3-0.8% of lactoferritin, 0.02-0.05% of lact-streptocin, 0.1-0.5% of lysozyme and balanced amout of water. The invention further discloses a method for keeping and cooling the pork by the biological fresh-keeping agent, which comprises the steps of: socking the cut cooled pork in the biological fresh-keeping agent for 0.5-1 minute or spraying the biological fresh-keeping agent on the surface of the cut cooled pork; cooling, draining for 3-5 minutes and packaging, storing under the temperature of 0-4 degrees centigrade. The biological fresh-keeping agent of the present invention achieves the advantages of long fresh-keeping time and good controlled quality, and has nutrition reinforcing and health caring function, but no side-effect to human body. The fresh keeping method in the invention has the advantages of easy operation, low cost, safety, environmental protection and no toxic or side effect.

The invention discloses a freezing method of fresh shrimp and a freeze protective agent of fresh shrimp. The protective agent comprises the first biological preservative and the second biological preservative; the first biological preservative is tea polyphenol solution; and the second biological preservative is a mixture of chitosan, sodium alginate, calcium chloride, lysozyme, propolis, lactoferritin, xylitol and sorbicolan. The protective agent is utilized for freeze preservation of fresh shrimps. The freeze protective agent of fresh shrimp disclosed by the invention is prepared from natural ingredients; the first biological preservative and the second biological preservative are combined, so that the fresh flavor and the original state of appearance of the aquatic products can be better protected; moreover, the freeze protective agent of fresh shrimp reduces nutrient loss, such as protein loss and the like, while inhibits bacteria production; thus, the freeze protective agent of fresh shrimp is capable of greatly prolonging the shelf life and the storage period of the aquatic products.

The invention relates to a polyurethane material with protein adsorption resistance and cell adhesion resistance and a preparing method thereof. The material comprises a substrate and a modification layer. The substrate is made of polyurethane. The modification layer is obtained by polymerizing a vinyl pyrrolidone monomer on the surface of the substrate by utilization of an atom transfer radical polymerization technology. The material is prepared by steps of: subjecting the polyurethane to isocyanic acid esterification, performing amination, fixing a bromine initiator, and initiating graft polymerization of the vinyl pyrrolidone on the surface of the material. The surface of the polyurethane material prepared by the method has good hydrophilicity, good resistance to bovine serum fibrinogen adsorption, bovine serum albumin adsorption and lysozyme adsorption, and a function of resisting cell adhesion of mouse fibroblast cells (L-929), so that the polyurethane material is suitable for preparing biomedical catheter materials with complex shape structures.

The present invention provides an antimicrobial peptide-lysozyme fusion protein. The protein comprises an antimicrobial peptide thanatin protein, 3GSA flexible peptide and T4 lysozyme protein from a terminal N to a terminal C. The preparation method comprises: obtaining a coding gene sequence comprising the antimicrobial peptide thanatin, 3GSA flexible peptide and T4 lysozyme by virtue of artificial synthesis; constructing a recombinant plasmid by taking pETDuet plasmid as a carrier; transforming the recombinant plasmid into BL21, and obtaining an expression strain by screening; putting the selected monoclone into a LB culture medium, and performing protein induction to obtain a fusion protein expressed in the supernatant; and purifying the fusion protein and cutting off an Sumo tag to obtain the objective antimicrobial peptide-lysozyme fusion protein. The protein provided by the present invention is proven to have remarkable effect in increasing the survival rate of piglets, reducing the diarrhea rate of nursery pigs, and decreasing the incidence of respiratory disease of nursery pigs, and is capable of being used for preparing related pharmaceutical preparations.

The invention discloses a fresh keeping agent containing essential oil, for fruits, and an application. The fresh keeping agent for fruits is characterized by being compounded from lysozyme, tea polyphenols, nisin, essential oil A, essential oil B, Vc and distilled water in a certain proportion. According to the fresh keeping agent for fruits, disclosed by the invention, the essential oil is optimized to be used as a main component of the fresh keeping agent, so that the fresh keeping agent has excellent effects of bacteriostasis and oxidation resistance and is applied to corrosion resistance and fresh keeping of the fruits, the fresh keeping and storage period of the fruits can be greatly prolonged, the fruits are guaranteed to maintain original quality in the fresh keeping period, and the quality of the fruits is effectively guaranteed. The fresh keeping agent for the fruits, disclosed by the invention is simple in compounding method, and convenient to apply; and the fresh keeping agent is completely compounded from natural components, and harmful chemical substances are not added, so that the fresh keeping agent is safe and environmental-friendly, and has an important industrial value.

Disclosed herein is a composition and a method for the treatment of otitis media and paranasal sinusitis using human defensins, lysozyme and / or lactoferrin as a new class of non-antibiotic antimicrobials. From studies of otitis media and paranasal sinusitis, it was observed that certain innate immune modulators were important in the bodies response to the infection. Therefore, these innate immune modulators, lysozyme, lactoferrin, and defensins were tested for use as a non-antibiotic treatment for infection, particularly infections such as otitis media and sinusitis.

The invention relates to a composite natural fresh-keeping agent for cold fresh meat and application of composite natural fresh-keeping agent. The composite natural fresh-keeping agent for the cold fresh meat is characterized by comprising tea polyphenol, chitosan, nisin, natamycin, lysozyme, oregano essential oil, vitamin C, vitamin E, erythorbic acid, a spice extract and water in certain proportions by weight, wherein the spice extract is obtained by extracting ginger, garlic, cinnamon, cardamom, clove and rosemary in certain proportions by weight through ethanol extraction. The composite natural fresh-keeping agent for the cold fresh meat is prepared from biological-source raw materials by a simple preparation method, has the advantages of non-toxicity, safety, broad antibacterial spectrum and the like, is convenient to operate during using and prominent in fresh-keeping effect, and has an important industrial value.

The invention provides a simple method for directly extracting microorganism macrogenome from an oil / gas pool environment sample, which comprises the following steps: an organic phase / water phase is extracted to obtain an microbe component in an oil sample / oil-water sample, vacuum filtering is carried out on the water phase enrichment microbes, cells are cracked by lysozyme, protease, sodium dodecyl sulfate (SDS) and the like, passes through the cetyl trimethyl ammonium bromide (CTAB) under high salt condition to remove organic macro-molecule components like protein and polysaccharide, extraction is carried out through nucleic acid and deposition is carried out, thereby the unbiased oil / gas pool microorganism macrogenome component can be obtained. The method of the invention has the advantages that the process is fast and simple, the cost is low, the whole extraction process can be completed in 10 hours; the obtained genome enables high purity, the ratio of OD260nm to OD280nm is between 1.7 and 2.0, the electrophoresis result detects that the integral genome possesses no degradation condition. The method of the invention aims at the oil / gas pool complex environments, and is suitable for extraction of other microbe genome in the soil and water body polluted by petroleum.

The invention belongs to the technical field of environment-friendly water treatment, particularly relates to a water purifying agent for wastewater treatment in rubber accelerator M production and further relates to a method for treating rubber accelerator M production wastewater by adopting the water purifying agent. The water purifying agent contains a flocculation water purifying agent, a microbial water purifying agent and an enzyme preparation, wherein the flocculation water purifying agent is prepared from polyaluminum chloride, polyacrylamide, ferrous sulfate and aluminum sulfate; the microbial water purifying agent is prepared from nitrobacterium powder, paracoccus denitrificans powder, thiobacterium powder, aniline degrading bacteria powder and flocculating bacteria powder; the enzyme preparation is prepared from pectinase, cellulase, oxidoreductase, lysozyme and alkaline proteinase. The water purifying agent and the method have the beneficial effects that by adopting the water purifying agent for wastewater treatment, disclosed by the invention, the water purifying effect is good, the water purifying speed is high, the safety is high, and no secondary pollution is caused; materials with adsorption capacity are used as raw materials and are assisted with microbes, so as to treat the wastewater, so that the range of use is wide, the transparency of treated water is extremely high, and recycling can be achieved.