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Novel feed additive and preparation method thereof

A feed additive and a new technology are applied in the field of new feed additives and their preparation, which can solve the problems that the preparation method is not suitable for industrial application, the scale production is difficult, the antibacterial spectrum of lysozyme is narrow, etc., so as to improve the quality of animal products and improve immunity strength, and the effect of improving disease resistance

Active Publication Date: 2009-09-16
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In contrast, there are few studies on the application of lysozyme in feed additives, and lysozyme itself has the disadvantages of narrow antibacterial spectrum and unstable performance, which is one of the reasons that limit its wide application in feed
[0004] Patent CN1666635 discloses a composition containing lysozyme and its use. The composition contains lysozyme, synergistic substances, coating materials and carriers. The components are made into products, the process is cumbersome, the cost is high, the scale production is difficult, and the preparation method is not suitable for industrial application
Patent US6221404B1 discloses a method that can enhance the feeding effect of probiotics - Bacillus cereus CIP5832, that is, adding probiotics and lysozyme or its salt at the same time can significantly improve the feeding effect of probiotics, but its limitations are Single strain of probiotics

Method used

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  • Novel feed additive and preparation method thereof
  • Novel feed additive and preparation method thereof
  • Novel feed additive and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Preparation of Bacillus subtilis powder

[0047] 1) Plate culture and rejuvenation: The Bacillus subtilis slant strains stored in the refrigerator are streaked and inoculated on the BPY plate medium containing 200ug / ml lysozyme by streaking, and cultivated at 37°C for 24 hours to rejuvenate the Bacillus subtilis , And form a single colony; pick a single colony on a fresh slant medium, cultivate it at 37°C for 36 hours, and place it in the refrigerator for later use;

[0048] 2) Preparation of first-level seeds: transfer the fresh slant strains of Bacillus subtilis cultured in step 1) to the fresh slant medium of eggplant bottles, and cultivate them at 37°C for 12 hours to make them in the mid- to late logarithmic stage, which are first-level seeds ;

[0049] The inclined plane medium is BPY solid medium;

[0050] 3) Preparation of secondary seeds: The primary seeds prepared in step 2) are made into a bacterial suspension with sterile water, and inoculated to a 1.6...

Embodiment 2

[0054] Example 2 Preparation of Lactobacillus powder

[0055] 1) Plate culture and rejuvenation: The Lactobacillus slant strains stored in the refrigerator are streaked on the MRS plate medium containing 200ug / ml lysozyme by streaking inoculation, and cultured at 37°C for 24 hours to rejuvenate the Lactobacillus, and Form a single colony; pick a single colony on a fresh MRS slant medium, culture it at 37°C for 36 hours, and place it in the refrigerator for later use;

[0056] 2) Preparation of first-level seeds: transfer the fresh slant strains of Lactobacillus cultured in step 1) to a 500mL Erlenmeyer flask containing 300ml MRS medium, and incubate at 37°C for 12h at 100rpm to make it logarithmic In the middle and late stages, it is the first seed;

[0057] 3) Preparation of secondary seeds: Inoculate the Lactobacillus seed liquid that has been cultured in step 2) into a 2.0L Erlenmeyer flask containing 1.6L MRS medium according to the 10% inoculum, and cultivate it at 37°C for 1...

Embodiment 3

[0061] Example 3 Preparation of Saccharomyces cerevisiae powder

[0062] 1) Plate culture and rejuvenation: Saccharomyces cerevisiae slant strains stored in the refrigerator are streaked on 98 plate medium by streaking inoculation, and cultured at 28°C for 36 hours to rejuvenate Saccharomyces cerevisiae and form a single colony; pick A single colony was cultured on fresh No. 98 slant medium, cultured at 28°C for 24-36 hours, and placed in a refrigerator for later use

[0063] 2) Preparation of first-level seeds: transfer the fresh slant strains of Saccharomyces cerevisiae cultured in step 1) to 500mL Erlenmeyer flasks containing 250ml of No.98 medium, and cultivate at 28°C for 12h at 150rpm to make it logarithmic In the middle and late stages, it is the first seed;

[0064] 3) Preparation of secondary seed: transfer the cultured Saccharomyces cerevisiae seed liquid in step 2) into a 2.0L Erlenmeyer flask containing 1.2L of No. 98 medium, and statically cultivate it at 28°C for 16 ...

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Abstract

The invention pertains to the field of microbial feed additives and relates to a novel feed additive containing lysozyme, probiotic powder and a carrier and a preparation method thereof, wherein the probiotic powder is bacillus subtillis powder and / or lactobacillus sp. powder and / or saccharomyces cerevisiae powder. The novel feed additive is the first time to add the lysozyme to a probiotic preparation which is then prepared to the novel feed additive. A product has and enhances the advantages of both the microecological preparation and the lysozyme, has better effects than the independently added microecological preparation or the lysozyme and can significantly improve weight increment and the feed conversion rate, the balance of intestinal flora as well as immunity and disease resistance. The novel feed additive has multi-effects of treatment, prevention, health care and immunity strengthening as well as simple preparation technology and is applicable to industrialized application.

Description

technical field [0001] The invention belongs to the field of microbial feed additives, and relates to a novel feed additive containing lysozyme, probiotic powder and a carrier and a preparation method thereof. Background technique [0002] In recent years, the incidence of livestock and poultry diseases has been increasing day by day, and the death rate caused by diseases has increased year by year, causing major economic losses to animal husbandry. At present, antibiotics are still used in the prevention and treatment of animal diseases, but due to the long-term and large-scale use of antibiotics, various disadvantages such as drug resistance, flora imbalance, decreased immunity, high disease recurrence rate, and drug residues are becoming more and more serious. Seriously endanger animal organisms and human health. At present, countries around the world have adopted measures to ban or restrict the use of antibiotics, such as: the European Union has banned the use of antibi...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/165A23K1/17A23K10/18A23K20/163A23K20/189A23K20/24A23K40/00
Inventor 孙占敏张玳华姚琨贾秋英刘宇赵军白玉卿赵雁青莫云
Owner BEIJING DABEINONG TECH GRP CO LTD
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