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Immobilized algal-bacterial symbiotic system for processing petroleum pollutants and application thereof

A technology for symbiosis of bacteria and algae, pollutants, applied in water pollutants, fixed on/in organic carriers, seawater treatment and other directions, can solve the problems of weakening photosynthesis, limiting microorganisms, low dissolved oxygen content in water, etc. No secondary pollution, high degradation efficiency

Inactive Publication Date: 2014-06-11
ZHEJIANG MARINE DEV RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the bacteria that use biological methods to treat marine oil pollution are aerobic bacteria, so the level of oxygen content greatly affects their oil degradation efficiency
However, due to the weakening of photosynthesis and the respiration of aquatic organisms in the water polluted by oil, the dissolved oxygen content in the water is low, which limits the improvement of the microbial degradation rate of oil.

Method used

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  • Immobilized algal-bacterial symbiotic system for processing petroleum pollutants and application thereof

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Effect test

Embodiment 1

[0028] The aseptic culture of embodiment 1 algal species

[0029] Chlorella algae fluid was pretreated with lysozyme / SDS, and then inoculated into BG11 culture fluid containing different combinations of antibiotics for aseptic culture.

[0030] The above pretreatment method is as follows: take the algae liquid cultured in BG11 culture medium for 7 days, centrifuge at 2000r / min for 10min, remove the supernatant, wash and centrifuge repeatedly with sterile distilled water for 3 to 5 times, add 1mL Tween80 (0.005%), 5 drops EDTA (0.1mol / L), 30mL sterile water, shake well and let stand for 10min. Add 1mL lysozyme and 5 drops of SDS, shake well, and let stand for 10min. After centrifuging to remove the supernatant, wash with sterile water 3 to 5 times.

[0031] Due to the different antibacterial mechanism of each antibiotic, the above 6 kinds of antibiotics were combined (Table 1) for aseptic culture of microalgae, and the method of detecting the sterilization effect with a plate...

Embodiment 2

[0039] The selection and cultivation of embodiment 2 petroleum degrading bacteria

[0040] The present invention selects 6 kinds of petroleum-degrading bacteria, which are purchased from China Marine Microorganism Culture Collection Management Center (MCCC), including 3 species of Pseudomonas, the strain numbers are 1A00241, 1A00269 and 1A00364, and 2 species of Bacillus, the strain number is 1A0008, 1A0009, 1 species of Achromobacter, the strain number is 1A04524, and the above-mentioned 6 kinds of petroleum degrading bacteria are named Y1, Y2, Y3, Y4, Y5, Y6 respectively.

[0041] The formula of petroleum-degrading bacteria medium used in the above step (2) is: petroleum 10g, NH 4 NO 3 3g, KH 2 PO 4 0.5g, K 2 HPO 4 0.5g, 2mL of trace element solution and 1000mL of water, the pH is 7.5.

[0042] The above trace element liquid components are: MgSO 4 0.4%, CuSO 4 0.1%, MnSO 4 0.1%, FeSO 4 ·H 2 O0.1% and CaCl 2 0.1%.

[0043] The culture time of the above step (2) i...

Embodiment 3

[0044] Embodiment 3 Preparation of composite carrier bacteria and algae gel ball

[0045] The formula (mass fraction) of the immobilized carrier is 0.3% sodium alginate, 20% PVA, 8% SiO 2 and 0.6%CaCO 3 .

[0046] Take 6 parts of 50mL immobilized carrier solution, add 25mL of bacteria solution containing Y1, Y2, Y3, Y4, Y5, Y6 strains and 25mL of chlorella algae solution to each part, mix well and drop boric acid solution with a syringe (4% boric acid concentration, pH=6.7), immobilized and cross-linked at 10°C for 20 hours, then took out the pellets and desalted them with deionized water for 2 hours, and named the six kinds of composite carrier bacteria and algae gel balls as B1 , B2, B3, B4, B5, B6, and stored in sterile seawater at 4°C for later use.

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Abstract

The invention belongs to the technical field of bioremediation of marine oil pollution, and relates to an immobilized algal-bacterial symbiotic system for processing petroleum pollutants. The immobilized algal-bacterial symbiotic system is prepared by the following steps: (1) selection and axenic cultivation of algae species, firstly carrying out lysozyme and sodium dodecyl sulfate (SDS) sterile pretreatment on chlorella algae fluid, and then inoculating into a BG11 culture solution containing antibiotics to carry out sterile cultivation, so as to obtain sterile chlorella algae fluid for later use; (2) selection and cultivation of petroleum degrading bacteria; (3) preparation of composite carrier bacteria algae gel balls, namely immobilizing the sterile chlorella algae fluid obtained in the step (1) and the petroleum degrading bacteria obtained in the step (2) by adopting sodium alga acid and polyvinyl acetate (PVA) as immobilized carriers, and finally obtaining the immobilized algal-bacterial symbiotic system. The immobilized algal-bacterial symbiotic system can float on the water to carry out degradation of petroleum pollution, and is free of secondary pollution, free of toxic action on microorganisms, rapid in reaction speed, stable and reliable in operation, less in microorganism loss, and free of separation.

Description

technical field [0001] The invention belongs to the technical field of bioremediation of marine oil pollution, and relates to an immobilized bacteria and algae symbiosis system for treating oil pollutants. Background technique [0002] The ocean is one of the three major ecosystems on the earth, and it is an important barrier to maintain the global ecological balance. While the ocean provides a large amount of resources for human beings, it is also facing more and more severe pollution. Among them, oil is the most important pollution in the marine environment. things. The main sources of marine oil pollution are oil transportation, oil field exploitation, coastal oil discharge and atmospheric petroleum hydrocarbon settlement. According to the statistics of relevant United Nations organizations, the annual marine oil pollution caused by human production and life is about 1×10 7 t, including the pollution caused by offshore oil well blowout accidents and tanker accidents, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/08C02F3/34C02F3/32C12R1/89C12R1/38C12R1/07C12R1/025C02F101/32C02F103/08
Inventor 徐珊郑斌陆阿定杨会成周宇芳胡建坤
Owner ZHEJIANG MARINE DEV RES INST
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