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1568 results about "Pseudomonas" patented technology
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Pseudomonas is a genus of Gram-negative, Gammaproteobacteria, belonging to the family Pseudomonadaceae and containing 191 validly described species. The members of the genus demonstrate a great deal of metabolic diversity and consequently are able to colonize a wide range of niches. Their ease of culture in vitro and availability of an increasing number of Pseudomonas strain genome sequences has made the genus an excellent focus for scientific research; the best studied species include P. aeruginosa in its role as an opportunistic human pathogen, the plant pathogen P. syringae, the soil bacterium P. putida, and the plant growth-promoting P. fluorescens.
P. aeruginosa mucoid exopolysaccharide specific binding peptides
InactiveUS6962813B2Enhance opsonization and phagocytosisEnhance cytocidal effectAnimal cellsImmunoglobulins against bacteriaDiseaseMonoclonal antibody
The present invention relates to peptides, particularly human monoclonal antibodies, that bind specifically to P. aeruginosa mucoid exopolysaccharide. The invention further provides methods for using these peptides in the diagnosis, prophylaxis and therapy of P. aeruginosa infection and related disorders (e.g., cystic fibrosis). Some antibodies of the invention enhance opsonophagocytic killing of multiple mucoid strains of P. aeruginosa. Compositions of these peptides, including pharmaceutical compositions, are also provided, as are functionally equivalent variants of such peptides.
Owner:THE BRIGHAM & WOMEN S HOSPITAL INC +1
Nanosilver-containing antibacterial and antifungal granules and methods for preparing and using the same
InactiveUS6379712B1Improve solubilityPreventing mold build-upPowder deliveryOrganic active ingredientsEscherichia coliDisease
The present invention relates to nanosilver-containing antibacterial and antifungal granules ("NAGs"). The NAGs have longlasting inhibitory effect on a broad-spectrum of bacteria and fungi, which include, but are not limited to, Escherichia coli, Methicillin resistant Staphylococcus aureus, Chlamydia trachomatis, Providencia stuartii, Vibrio vulnificus, Pneumobacillus, Nitrate-negative bacillus, Staphylococcus aureus, Candida albicans, Bacillus cloacae, Bacillus allantoides, Morgan's bacillus (Salmonella morgani), Pseudomonas maltophila, Pseudomonas aeruginosa, Neisseria gonorrhoeae, Bacillus subtilis, Bacillus foecalis alkaligenes, Streptococcus hemolyticus B, Citrobacter, and Salmonella paratyphi C. The NAGs contain ground stalk marrow of the plant Juncus effusus L. which has been dispersed with nanosilver particles. The nanosilver particles are about 1-100 nm in diameter. Each of the nanosilver particles contain a metallic silver core which is surrounded by silver oxide. The present invention also provides a process for making the NAGs. The NAGs can be used in a variety of healthcare and industrial products. Examples of the healthcare products include, but are not limited to, ointments or lotions to treat skin trauma, soaking solutions or cleansing solutions for dental or women hygiene, medications for treating gastrointestinal bacteria infections, sexual related diseases, and eye diseases. Examples of industrial products include, but are not limited to, food preservatives, water disinfectants, paper disinfectants, construction filling materials (to prevent mold formation).
Owner:LEGEND WIN FINANCE
Recombinant antibodies and immunoconjugates targeted to CD-22 bearing cells and tumors
InactiveUS7541034B1Growth inhibitionBacteriaPeptide/protein ingredientsPseudomonas aeruginosa exotoxin APseudomonas
Methods and compositions relating to recombinant anti-CD22 antibodies with high binding affinity, and immunoconjugates comprising the anti-CD22 antibody linked to a therapeutic agent such as a Pseudomonas exotoxin or a detectable label. The invention provides diagnostic methods, and means to inhibit the growth of malignant B cells.
Owner:UNITED STATES OF AMERICAN AS REPRESENTED BY THE SEC OF THE DEPT OF HEALTH & HUMAN SERVICES
Compositions and methods for treating cystic fibrosis
Particular aspects provide electrokinetically-generated fluids (e.g., electrokinetically-generated gas-enriched fluids and solutions), and therapeutic compositions and methods comprising use thereof in treating at least one symptom of cystic fibrosis. In particular embodiments, at least one symptom of cystic fibrosis treated by the present invention include inhibition of Pseudomonas infection, synergy with tobramycin (including TOBI) for use against bacterial infection, and synergy with a bronchiodilator. In particular embodiments, the electrokinetically-generated fluids or therapeutic compositions and methods comprise combination with other therapeutic agents (e.g., antibiotics, albuterol, budesonide, etc.). In certain aspects, the methods comprise regulating or modulating intracellular signal transduction by modulation of at least one of cellular membranes, membrane potential, membrane proteins such as membrane receptors, including but not limited to G protein coupled receptors, and intercellular junctions (e.g., tight junctions, gap junctions, zona adherins and desmasomes).
Owner:REVALESIO CORP
Topical compositions containing probiotic bacillus bacteria, spores, and extracellular products and uses thereof
The present invention discloses compositions derived from an isolated Bacillus species, spores, or an extracellular product of Bacillus coagulans comprising a supernatant or filtrate of a culture of said Bacillus coagulans strain, suitable for topical application to the skin or mucosal membranes of a mammal, which are utilized to inhibit the growth of bacterium, yeast, fungi, virus, and combinations thereof. The present invention also discloses methods of treatment and therapeutic systems for inhibiting the growth of bacterium, yeast, fungi, virus, and combinations thereof, by topical application of therapeutic compositions which are comprised, in part, of isolated Bacillus species, spores, or an extracellular product of Bacillus coagulans comprising a supernatant or filtrate of a culture of said Bacillus coagulans strain. In addition, the present invention also discloses compositions, methods of treatment, and therapeutic systems for inhibiting the growth of bacterium, yeast, fungi, virus, and combinations thereof, comprising an extracellular product of Pseudomonas lindbergii comprising a supernatant or filtrate of a culture of said Pseudomonas lindbergii strain.
Owner:GANEDEN BIOTECH
Codon optimization method
A heterologous expression in a host Pseudomonas bacteria of an optimized polynucleotide sequence encoding a protein.
Owner:DOW AGROSCIENCES LLC
Compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof
InactiveCN106434430AEliminate pollutionAchieve biodegradableFungiBacteriaEcological environmentBacillus megaterium
The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.
Owner:中山市润泽生物科技有限公司
Nanosilver-containing antibacterial and antifungal granules and methods for preparing and using the same
Owner:LEGEND WIN FINANCE
Microorganism bacterium composition, special biological organic fertilizer for tobacco containing the same, preparation and use method for the microorganism bacterium composition
InactiveCN101054568AImprove coordinationImprove fertilizer efficiencyBio-organic fraction processingBacteriaBiotechnologyBacillus megaterium
The present invention provides a microbe composition, tobacco-dedicated biological organic fertilizer containing the composition, its usage method. The microbe composition comprises aztobacter sp., pseudomonas fluorescence, Bacillus megaterium, bacillus mucilaginosus and bacteria capable of preventing and treating plant disease and insect pests. The microbe composition is mixed according to proportion with oligochitosan, lignite, grass charcoal, and bean cake and fermented to make bacteria powder fertilizer, and further added with potassium-enriching shale, bentonite and water-keeping agent to make bacteria granule fertilizer. The tobacco-dedicated biological organic fertilizer contains the above-mentioned bacteria powder fertilizer and granule fertilizer which contains natural mineral and good organic substance, C / N value being large, which fit the growth rule of tobacco plant and promote potassium fertilizer efficiency and possesses long-lasting potassium-supply capability and provides effective ingredient for tobacco.
Owner:周文彩
Novel aldolase and production process of substituted alpha-keto acids
4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and / or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.
Owner:AJINOMOTO CO INC
Over-expression of extremozyme genes in pseudomonads and closely related bacteria
InactiveUS20050130160A1Increase cell densityImprove the level ofSugar derivativesBacteriaPseudomonasOver expression
Owner:DOW GLOBAL TECH LLC
Expression of mammalian proteins in Pseudomonas fluorescens
The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances Processes for improved production of isolated mammalian, particularly human, proteins are provided.
Owner:PELICAN TECH HLDG INC
Plant restoration method for heavy metal pollution of soil
InactiveCN1593797AImprove extraction efficiencyNo secondary pollutionContaminated soil reclamationRestoration methodSoil heavy metals
Owner:SOUTH CHINA UNIV OF TECH
Modified pseudomonas exotoxin a
ActiveUS20150099707A1Increased serum half-lifeOptimize treatment planOrganic active ingredientsBacteriaPseudomonas aeruginosa exotoxin APseudomonas
The invention provides a Pseudomonas exotoxin A (PE) comprising an amino acid sequence having a substitution of one or more B-cell and / or T-cell epitopes. The invention further provides related chimeric molecules, as well as related nucleic acids, recombinant expression vectors, host cells, populations of cells, and pharmaceutical compositions. Methods of treating or preventing cancer in a mammal, methods of inhibiting the growth of a target cell, methods of producing the PE, and methods of producing the chimeric molecule are further provided by the invention.
Owner:UNITED STATES OF AMERICA
Mutated anti-cd22 antibodies and immunoconjugates
ActiveUS7982011B2Immunoglobulin superfamilyAntibody mimetics/scaffoldsAntiendomysial antibodiesCancer cell
Recombinant immunotoxins are fusion proteins composed of the Fv domains of antibodies fused to bacterial or plant toxins. RFB4 (Fv)-PE38 is an immunotoxin that targets CD22 expressed on B cells and B cell malignancies. The present invention provides antibodies and antibody fragments that have improved ability to bind the CD22 antigen compared to RFB4. Immunotoxins made with the antibodies and antibody fragments of the invention have improved cytotoxicity to CD22-expressing cancer cells. Compositions that incorporate these antibodies into chimeric immunotoxin molecules that can be used in medicaments and methods for inhibiting the growth and proliferation of such cancers. Additionally, the invention provides a method of increasing the cytotoxicity of forms of Pseudomonas exotoxin A (“PE”) with the mutation of a single amino acid, as well as compositions of such mutated PEs, nucleic acids encoding them, and methods for using the mutated PEs.
Owner:UNITED STATES OF AMERICA
Expression of mammalian proteins in Pseudomonas fluorescens
The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances Processes for improved production of isolated mammalian, particularly human, proteins are provided.
Owner:PELICAN TECH HLDG INC
Composite bacterium agent for organic material fermentation
The invention relates to an organic materials zymolysis complex bacillus agent in the field of zymolysis bacillus agent technology. It comprises: low temperature bacteria, Bacillus cereus, gliotoxin, pseudomonas, slim streptomycete, high temperature bacteria and high temperature actinomyces with the weight ration 4:2:1:1:3:2:2. The low temperature bacterium is enzyme yeast with the heat resisting temperature below 20 deg. The high temperature bacterium is enzyme yeast with the heat resisting temperature below 70 deg. The heat resisting temperature below 70 deg of the high temperature actinomyces bellows 70 deg.
Owner:青岛地恩地生物科技有限公司
Kit for quickly detecting 15 pneumonia pathogenic bacteria
ActiveCN107338315AMicrobiological testing/measurementMicroorganism based processesBacteroidesStaphylococcus aureus
The invention discloses a kit for quickly detecting 15 pneumonia pathogenic bacteria. The kit can detect streptococcus pneumoniae, staphylococcus aureus, haemophilus influenzae, mycoplasma pneumoniae, pseudomonas aeruginosa, baumanii, enterococcus faecalis, enterococcus faecium, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, stenotrophomonas maltophilia, burkholderia cepacia, legionella pneumophila and chlamydia pneumoniae which cover clinically common pneumonia pathogenic bacteria difficult to culture. 16S rDNA and specific gene sequences corresponding to the pneumonia pathogenic bacteria are detected by combining gene chips with multiple asymmetric PCR reactions, and the categories of the bacteria in a to-be-detected sample are identified in genus and species. The kit makes up for the defect that current clinical detection of pneumonia pathogenic bacteria is not in time or comprehensive and a novel detection means for early diagnosis and early treatment of patients suffering from pneumonia is provided.
Owner:GENERAL HOSPITAL OF PLA +1
Bacillus amyloliquefaciens JK6 and biological fertilizer and application
The invention discloses bacillus amyloliquefaciens JK6 and biological fertilizer and application. The serial number of strain preservation is CGMCC No. 10658 and is stored in the China general microbiological culture collection center on March 23, 2015. The bacillus amyloliquefaciens JK6 is mainly used for biological prevention and control of pseudomonas solanacearum and is provided with an iron-production carrier, a biological membrane, protease, catalase, cellulose and IAA characteristics, multiple fungal pathogens of banana wilt pathogen, litchi anthrax germs, litchi frost phytophthora pathogens, rice blast fungus, cucumber wilt pathogen and the like, and growth of lettuce plants is obviously promoted; the K6 strain can be successfully planted, and disease preventing functional genes of yndj, srfAB, fend and ituC are expressed in soil; the strain is high in reproduction speed, simple in production technology, high in adverse resistance, easy to store and favorable for industrialized production, the biological fertilizer is prepared with the strain, soil-born diseases can be prevented and controlled, growth of the plants can be promoted, and application prospect is broad.
Owner:SOUTH CHINA AGRI UNIV
Solid-chemical composition for sustained release of organic substrates and complex inorganic phosphates for bioremediation
InactiveUS6620611B2Improve solubilityIncreasing speed and effectivenessBacteriaWater treatment compoundsPseudomonasTrichoderma spp
A slow-release solid chemical composition for environmental bioremediation is provided. The composition comprises a source of soluble organic substrates which include sugars, soluble organic polymers and mixtures of them in an amount of 7% to 90%, insoluble organic substrates an amount of 10% to 70%, complex inorganic phosphates in an amount of 0.5% to 7% and soluble organic salts in an amount of 2% to 70%. The insoluble organic substrates include fibrous plant materials, starches, cellulosic materials and mixtures of these substrates. The complex inorganic phosphates include ringed metaphosphates, linear polyphosphates and mixtures. The organic salts include lactates, formates, acetates, citrates, etc. Also the composition further comprises microorganisms which include Bacillus spp., Rhizobium spp., Bradyrhibzobium spp., Fibrobacter spp., Clostridium spp., Pseudomonas. spp., Geobacter spp., Arthrobacter spp., Nocardia, spp., aspergillus spp., Trichoderma spp., Candida spp., Yarrowia spp. and combinations of these microorganisms. The composition can be prepared in various forms, including granules, briquettes, pellets, tablets or capsules.
Owner:HINCE ERIC CHRISTIAN MR
Aldolase and production process of substituted alpha-keto acids
Owner:AJINOMOTO CO INC
Preparation of microcapsules of viable microbe biopesticide
The invention relates to microcapsules prepared from viable microbes such as pseudomonas, bacillus and paenibacillus serving as main active components, belonging to the technical field of microorganism and biochemistry. The method for preparing microcapsules of viable microbe biopesticide comprises the following steps of: adsorbing microbial fermentation culture solution through an adsorption effect to prepare capsule cores and solidifying the capsule cores, and adding wall materials, so as to prepare a microcapsule preparation. The microcapsule preparation prepared by adopting the method has high content of viable microbes, high storage property and strong high-temperature and ultraviolent resistances, and stable and good prevention and control effect on plant diseases and insect pests.
Owner:成都特普生物科技股份有限公司
Microbial agent for purifying river wastewater and preparation method of microbial agent
The invention relates to a microbial agent for purifying river wastewater and a preparation method of the microbial agent. The microbial agent comprises the following components in parts by weight: 20-40 parts of bacillus natto, 10-30 parts of nitrobacteria, 5-15 parts of white-rot fungi, 5-15 parts of pseudomonas and 10-40 parts of photosynthetic bacteria. The microbial agent is well-adapted and has the capabilities of fast forming dominant bacterial communities and replenishing indigenous rhizobia to the water body; and the microbial agent can be used for reducing the COD (Chemical Oxygen Demand), NH3-H, total nitrogen, TP (Total Phosphorus) and the like, removing odor and color and producing high-activity bio-enzyme to suppress the growth of harmful bacteria and acceleratively decompose residual baits as well as animal and plant residues, and a remarkable water quality purifying effect is achieved.
Owner:浙江一清环保工程有限公司
High level expression of recombinant toxin proteins
ActiveUS20110287443A1High yieldQuality improvementAntibody mimetics/scaffoldsBacteria peptidesBacteroidesHigh level expression
The present invention relates to the field of recombinant toxin protein production in bacterial hosts. In particular, the present invention relates to production processes for obtaining high levels of a recombinant CRM197, Diphtheria Toxin, Pertussis Toxin, Tetanus Toxoid Fragment C, Cholera Toxin B, Cholera holotoxin, and Pseudomonas Exotoxin A, from a bacterial host.
Owner:PFENEX
Biological organic fertilizer prepared by aerobic fermentation of biogas residue and preparation method thereof
InactiveCN103848698AWon't burnDecompose thoroughlyBio-organic fraction processingWaste based fuelMicrobial agentPseudomonas
The invention provides a biological organic fertilizer prepared by aerobic fermentation of a biogas residue and a preparation method thereof. The preparation method comprises the steps: mixing the biogas residue and a swelling agent to form a fermentation raw material, inoculating a compound microbial agent in the fermentation raw material, uniformly mixing for fermenting, and adding urea, diammonium phosphate, a potassium chloride inorganic salt, brown nitrogen-fixing bacteria, mycorrhiza fungi, silicate bacteria, photosynthetic bacteria acetic bacteria, bifidobacterium, and saccharomycetes after the fermentation is completed to prepare the biological organic fertilizer, wherein the compound microbial agent comprises strains of bacillus, pseudomonas, staphylococcus, streptomyces, penicillium, aspergillus and trichoderma. The compound microbial agent prepared by using the biological organic fertilizer has a pertinence to aerobic fermentation of the biogas residue, and is capable of effectively increasing the fermentation rate of the biogas residue, shortening the fermentation time and realizing high-additional value production, innocent treatment and recycling of the biogas residue; and the problem of resources and environment of villages and small towns is solved, and the rapid development of construction of new countryside and towns and cities can be promoted.
Owner:青岛福瑞斯生物能源科技开发有限公司
Pseudomonas. chlororaphis subsp. Aurantiaca Pa40 and application thereof
The invention provides a Pseudomonas.chlororaphis subsp.Aurantiaca Pa40, preservation number of which is CGMCCNo.2764. The strain generates HCN, prolease and siderophore relative to antibacterium and has plate board inhibition effect on a majority of tested plant pathogenic fungi and a part of tested plant pathogenic bacteria. Control effect of the biocontrol strain Pa40 on rhizoctonia solani is detected by taking wheat as an indicator plant on the plate, in the greenhouses and in the fields; a result shows that the control effects of the Pa40 on the rhizoctonia solani on the plate bed, in the greenhouse and in the fields are respectively 53.97%, 68.10% and 72.46%; wherein the control effects on the rhizoctonia solani in the greenhouses and fields are higher than that of validamycin.
Owner:CHINA AGRI UNIV
Preparation method and application for compound fungus agent for degrading organic matter
InactiveCN102344899AImprove biological activityImprove adsorption capacityBacteriaWater contaminantsBiotechnologyBenzoic acid
The invention relates to a preparation method for a compound fungus agent for specifically degrading an organic matter for a biological enhancing water treatment system. The compound fungus agent is added into a biological treatment process, has high biological activity, a stable effect and low cost, is easy to adsorb on the surface of a carrier and is difficult to lose. The preparation method issimple and practical, and has a short preparation period and low cost. The compound fungus agent comprises the following four strains: 20 to 30 percent of pseudomonas-stutzeri, 20 to 30 percent of pseudomonas putida, 10 to 20 percent of pseudomonas-pertucinogena and 30 to 50 percent of bacillus subtilis. The compound fungus agent is prepared by combining and screening the strains, performing slant culture and primary culture, mixing according to the proportion, and performing secondary cycle culture. The preparation method is widely used in a biological enhancing water treatment process, has a long-term and stable operation period, and has specific degradation ability for toxic and harmful organic pollutants such as halogenated hydrocarbons, phenol, benzoic acid, aromatics, polycyclic aromatic hydrocarbon, heterocyclic compounds, phthalic acid ester and the like.
Owner:SHENYANG JIANZHU UNIVERSITY
Bacillus coagulans HEW-B379 with probiotic effect, and application thereof
ActiveCN106011036AStrong heat resistanceStrong fermentation abilityAntibacterial agentsBacteriaEscherichia coliFeed conversion ratio
The invention provides a Bacillus coagulans HEW-B379 with a probiotic effect. The above strain is named as HEW-B379, and the preservation number of the strain is CGMCC No.12553. The Bacillus coagulans HEW-B379 has a substantial probiotic property, and can effectively inhibit growth breeding of enteropathogenic Escherichia coli, Staphylococcus aureus, Salmonella typhi, salmonella, Shigella, Proteus species, Shewanella putrefaciens and Pseudomonas aeruginosa. The Bacillus coagulans HEW-B379 has strong stress resistance, can resist high temperature and simulated gastric juice and simulate bile salt environment, can keep the survival rate of 99-100%, and can effectively adjust microbial balance of animal intestinal tracts, inhibit growth of harmful microbes, promote nutrition absorption of animals, improve the conversion rate of a feed and improve the productivity of the animals.
Owner:BEIJING HESWOF BIOTECH CO LTD
Microorganism advanced treatment for oil refining waste water
ActiveCN101148293AAvoid pollutionStable waterTreatment using aerobic processesBacteriaInitial treatmentBacillus megaterium
The present invention relates to deep treating process of refinery waste water after initial treatment. Refinery waste water is deeply treated biologically with microbial flora comprising Pseudomonas sp., Zoogloea sp., Plesiomonas sp., Bacillus, Bacillus megaterium and Rhodopseudomonas globiformis in a biological aeration filtering pond and / or a bioreaction pond while controlling the intake water pH value in 6-9. The deep treating process has simple path, high safety, low power consumption, low running cost and other advantages, and the water output can reach the reuse standard stably including CODcr not more than 60 mg / l, oil not more than 2 mg / l, NH3-N not more than 2 mg / l and SS not more than 10 mg / l.
Owner:JIANGSU BODA ENVIRONMENTAL PROTECTION
Biological-Based Catalyst to Delay Plant Development Processes
ActiveUS20080236038A1Extended shelf lifeFacilitating longer-distance transportationBiocideBacteriaBacteroidesBrevibacterium ketoglutamicum
The present invention is directed to methods for delaying a plant development process comprising exposing a plant or plant part to one or more bacteria or enzymes. In specific embodiments, the one or more bacteria are selected from the group consisting of Rhodococcus spp., Pseudomonas chloroaphis, Brevibacterium ketoglutamicum, and a mixture comprising any combination of these bacteria. Apparatuses for delaying a plant development process comprising a catalyst that comprises one or more of the above bacteria.
Owner:CROWPIERCE TECH LLC
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