478 results about "Fermentation rate" patented technology

The invention discloses a multi-cycle stirring egg-shaped anaerobic fermentation system. The system comprises a fermentation tank body and a driving device arranged on the fermentation tank body, wherein the fermentation tank body is egg-shaped, the center of the inner cavity of the fermentation tank body is provided with an inner barrel type spiral stirrer, the outside of the inner barrel type spiral stirrer is provided with a stirrer inner barrel, a temperature heating device is arranged between the wall of the fermentation tank body and the stirrer inner barrel, the bottom of the inside ofthe tank is provided with an umbrella-shaped gas flow distribution device; the upper part of the fermentation tank body is provided with a vent pipe and a pressure control device, the bottom of the fermentation tank body is provided with an umbrella-shaped gas flow distribution device gas inlet pipe and a mud-discharging and sand-discharging outlet; and the side wall of the fermentation tank bodyis communicated with a biogas circulation system, a biogas slurry circulation system, a gas-liquid circulation system, a heating system, a temperature control system and a biogas residue and biogas slurry storage pool respectively. The multi-cycle stirring egg-shaped anaerobic fermentation system adopts multi-cycle stirring to increase the stirring efficiency of the system; and the anaerobic fermentation environment is improved, the fluid is mixed evenly, the temperature difference slightly fluctuates, the energy consumption of the system is low, the fermentation rate is high and the system is suitable for popularization and application.

A process for producing ethanol from a fibrous lignocellulosic raw material. After pre-treatment of the raw material, the fibrous fraction is first hydrolysed at high consistency and then the modified material is subjected simultaneously to continued hydrolysis with a cellulase and to ethanol fermentation in a fermentation mixture. Fermentation is continued to convert an essential portion of the available cellulose into ethanol, then a liquid fraction containing solubilized hemicelluloses is added to the fermentation mixture and fermentation continued. By means of the invention, high fermentation rates, high ethanol concentrations and low ethanol production costs can be attained.

The invention provides a biologically-fermented complete compound feed. In the complete compound feed, a protein feed and an energy feed are fermented respectively and are matched with a premix compound, or the protein feed and the energy feed are matched together to be fermented, and are matched with the premix compound, or the protein feed, the energy feed and the premix compound are matched together to be fermented, wherein the premix compound accounts for 2 to 6 percent of the total weight of the feed; and the fermentation is performed by adding feed-level microbes which are beneficial tothe survival or growth period of raised animals into the feed or adding feed-level enzymic preparations into the feed, wherein the fermentation rate is between 50 and 100 percent, and the fermented feed contains a large number of fermentation products and beneficial bacterial colonies. The complete compound feed has the advantages of high utilization rate of absorption, growth promotion, disease prevention, disease resistance and high cost performance and is easy to digest, and safe, so the feed industry and the cultivation industry have new development space.

The invention provides a biological organic fertilizer prepared by aerobic fermentation of a biogas residue and a preparation method thereof. The preparation method comprises the steps: mixing the biogas residue and a swelling agent to form a fermentation raw material, inoculating a compound microbial agent in the fermentation raw material, uniformly mixing for fermenting, and adding urea, diammonium phosphate, a potassium chloride inorganic salt, brown nitrogen-fixing bacteria, mycorrhiza fungi, silicate bacteria, photosynthetic bacteria acetic bacteria, bifidobacterium, and saccharomycetes after the fermentation is completed to prepare the biological organic fertilizer, wherein the compound microbial agent comprises strains of bacillus, pseudomonas, staphylococcus, streptomyces, penicillium, aspergillus and trichoderma. The compound microbial agent prepared by using the biological organic fertilizer has a pertinence to aerobic fermentation of the biogas residue, and is capable of effectively increasing the fermentation rate of the biogas residue, shortening the fermentation time and realizing high-additional value production, innocent treatment and recycling of the biogas residue; and the problem of resources and environment of villages and small towns is solved, and the rapid development of construction of new countryside and towns and cities can be promoted.

A process for producing ethanol from a fibrous lignocellulosic raw material. After pre-treatment of the raw material, the fibrous fraction is first hydrolysed at high consistency and then the modified material is subjected simultaneously to continued hydrolysis with a cellulase and to ethanol fermentation in a fermentation mixture. Fermentation is continued to convert an essential portion of the available cellulose into ethanol, then a liquid fraction containing solubilized hemicelluloses is added to the fermentation mixture and fermentation continued. By means of the invention, high fermentation rates, high ethanol concentrations and low ethanol production costs can be attained.

The invention discloses a livestock and poultry feces organic fertilizer fermentation compound functional package, which comprises a compound functional package A and / or a compound functional packageB. The compound functional package A comprises a compound microbial agent, a compound enzyme preparation, humic acid, white spirit vinasse powder and zeolite powder; the compound functional package Bcomprises isomaltooligosaccharide, molasses meal, monopotassium phosphate, magnesium sulfate, ferrous bisglycinate, manganese bisglycinate, bamboo vinegar powder and citric acid. The package A and thepackage B are combined, the fermentation speed can be greatly increased, quick heating deodorization and thorough decomposition can also be achieved in the low-temperature season, diseases and insect pests and antibiotic residues can be effectively removed, the quality is stable, the quality guarantee period is long, and practicability is high. The invention further discloses a preparation method and application of the compound functional package, the compound functional package is used for livestock and poultry feces organic fertilizer fermentation, the harmless treatment efficiency is high, resource utilization is high, an obtained organic fertilizer product is high in functionality and comprehensive in nutrition, and has no toxicity, stink, harm and pollution, and the organic fertilizer use standard requirement is met.

The invention discloses a grapefruit vinegar, a preparation method thereof and a production method of beverage thereof, and belongs to the technical field of fermenting food processing. The method comprises the following steps: by using S.cerevisiae HY16 CGMCC No.3450 as the strain and grapefruit juice as the raw material, carrying out alcoholic fermentation to prepare the grapefruit wine; fermenting the grapefruit wine into the grapefruit vinegar by using the acetic acid bacterial strain; and sealing, ageing, filtering, filling and sterilizing to obtain the grapefruit vinegar beverage. The invention adopts the self-bred new strain and new process, thereby increasing the fermentation rate, shortening the production cycle and obviously enhancing the quality of the fruit vinegar product; and the invention provides an effective way for the development and utilization of grapefruit resources. The invention can be popularized in the industries of fermentation and food beverages.

The invention discloses a new preparation process of an agaricus bisporus culture material. The product belongs to the field of edible mushroom, and the invention is characterized by relating to the agaricus bisporus culture material. The invention aims at solving the problem that a new tunnel aeration fermentation method is provided to produce the agaricus bisporus culture material. The method has the advantages that: 1, wheat straw and chicken manure are taken as main raw materials, the raw materials are easily available, nitrogen content in the chicken manure is high, and heat productivityis high; 2, automatic mechanical continuous operation is adopted, manpower is saved, and mass production can be realized; 3, heat distribution in the culture material is uniform, fermentation temperature in the material is basically consistent, and quality can be guaranteed; and 4, fermentation speed is high, production efficiency is high, and less sundry bacteria are contained. The raw materialsused for producing the agaricus bisporus culture material comprise the wheat straw, the chicken manure, calcium carbonate, inorganic compound fertilizer, calcium superphosphate, lime powder and gypsum powder, and the product is used for planting agaricus bisporus.

An improved method and device is accomplished for ethanol production using an in-line extraction of ethanol by ultrasonic atomization, thereby removing the effect of the ethanol inhibition factor that adversely affects the rate and yield. The in-line removal of ethanol as it is formed increases the fermentation rate, improves the yield and uses 20-25% of the energy required as compared to thermal distillation processes currently used. The improved method makes sure that ultrasonic vibration does not deactivate enzymes to any significant level. The elimination of the effect of the ethanol inhibition factor allows for a continuous fermentation process as opposed to the costly, time-consuming repeated batch processes.

The invention discloses a composite leavening agent combining xylanase with multiple strains and a method for fermenting a straw feed. The leavening agent consists of the following components in part by weight: 4 parts of xylanase, 2 parts of lactobacillus acidophilus, 2 parts of propionibacterium, 2 parts of saccharomyces cerevisiae, 3 parts of bacillus subtilis, 1 part of lactobacillus plantarum and 1 part of bacillus coagulans, wherein the xylanase can quickly degrade cell walls of straw plants and release nutrient substances; and under the synergistic effects of a plurality of microorganisms, fermentation speed is increased, fermentation period is shortened, fermentation activity is enhanced, protein conversion rate is increased and fermentation cost is lowered. The prepared fermented straw feed has low cellulose content, high protein content, high viable count, long retention period, soft and wet feel, strong acid incense, high palatability and high nutritional value. The problem of low resource utilization ratio of crops such as straws and the like is solved; simultaneously, environmental pollution is reduced; and the leavening agent and the method have high economic benefit and social benefit.

The invention discloses a new bacterial strain for quick flavor enhancement of flue-cured tobacco, which is named Bacillus LBT1.0003 and is preserved at CGMCC No.5331 in China General Microbiological Culture Collection Center (CGMCC). Obtained from separating and selecting from surfaces of alcoholized tobacco, the bacterial strain is easy to cultivate and is good in flavor enhancement effect and quick in fermentation speed. After the bacterial strain is used in fermentation of flue-cured tobacco, the physical and chemical indicators of the flue-cured tobacco (like total sugar, reducing sugar and potassium) are obviously improved. Results from a smoke panel test show that after the tobacco is processed by the microorganism, the taste quality of the tobacco is greatly improved, the smoke concentration is increased and the fragrance is increased, and the tobacco has soft and delicate fragrance, has the smell of stale tobacco and has less irritative and spicy taste.

The invention belongs to the technical field of microorganism fermentation engineering, and in particular relates to a method for producing high-concentration ethanol by fermenting fresh sweet potato raw materials with slag. The method of the invention comprises three steps, namely fermentation raw material preparation, seed liquor preparation and fermentation. In the process of fermentation raw material preparation, composite pretreatment enzyme solution which contains composite pectinase, cellulose and xylanase is added; and the high-concentration ethanol is obtained after the fermentation. The method has the characteristics of simple process, high operability, high fermentation speed, high final ethanol concentration, high fermentation efficiency, low residual sugar and effective reduction of ethanol production cost.

The invention relates to a biomass pretreatment method, a method and an equipment for preparing firedamp by biomass anaerobic fermentation, comprising the following steps of: 1) treating the biomass as short sections of 3-40mm; 2) soaking the short sections in water at the temperature of 15-80 DEG C for 15-90min; 3) tearing up the soaked short sections into fragments; 4) leading fragments to carry out a hydrothermal reaction under a heating or heating and pressurizing condition; and 5) quenching the straws to obtain saccharification solution or saccharification slag after the hydrothermal reaction. The method improves the biological degradability by the pre-treatment of the straws, quickens the fermentation speed, greatly improves the fermentation efficiency by respectively fermenting the pre-treated solid and liquid, avoids the exhaust of a large amount of waste liquid by the circular fermentation of hydrothermal reaction of the fermentation liquid, leads the whole system to run at high efficiency and greatly improves the conversion rate of the biomass.

The invention relates to a building method of genetic engineering acetic acid bacteria of recombinantly expressing coenzyme PQQ (pyrroloquinoline quinone) synthetic proteins. The PQQ biosynthetic proteins are PqqA, PqqB, PqqC, PqqD, PqqE, and functional equivalents thereof. An alcohol dehydrogenase promoter from acetobacter pasteurianus and a coenzyme PQQ synthetic protein gene cluster pqqABCDE sequence are orderly connected into plasmids which can be stably copied in the acetic acid bacteria, so as to recombine pBBR-padh-pqq; recombinant plasmid pBBR-padh-pqq is transferred into the acetic acid bacteria, so as to obtain the genetic engineering acetic acid bacteria of overexpressing the coenzyme PQQ synthetic proteins. Thus, the concentration of in vivo coenzyme PQQ of the acetic acid bacteria in the fermentation process of acetic acid is improved. Acetic acid fermentation is carried out by using the genetic engineering bacteria disclosed by the invention, the fermentation delay phase can be shortened, and the fermentation rate of the acetic acid is increased. Thus, the production cost is reduced.

The invention discloses a preparation method of molasses for recycling. The preparation method comprises the following steps of: firstly, preparing a culture medium, a molasses culture solution tank A, a molasses culture solution tank B, a molasses culture solution tank C as well as a yeast fermented inoculum; secondly, selecting different saccharomycetes and combining for fermenting to obtain alcohol and alcohol slops, wherein the fermenting is divided into three phase; and thirdly, after the molasses is fermented, collecting the alcohol, washing indecomposable macromolecular organic matters in residual alcohol slops with water; decomposing the washed macromolecular organic matters to form soluble bioactive micro-molecules by adopting a chemical activation mode; and chelating and mixing the soluble bioactive micro-molecules to form organic soluble fertilizer with different formulas. According to the preparation method and the characteristics of different saccharomycetes, in particular yeast which can be used for fermenting non-fermentable sugar, the fermentation rate of cane molasses for producing the alcohol is increased by fully utilizing sugers of various types in the molasses, so that the production efficiency of the equipment and the labor efficiency can be increased and the energy consumption is reduced.

The invention discloses a saccharomyces cerevisiae and application thereof, which relate to a microorganism and a technique of brewing fruit wine. The strain of the saccharomyces cerevisiae is also used for preparing a sea-buckthorn fruit wine by using sea-buckthorns or sea-buckthorn fruits as raw materials so as to produce a high-quality sea-buckthorn fruit wine. The separation source of the strain of the saccharomyces cerevisiae comprises a sea-buckthorn fruit juice natural fermentation broth, peels and pulps of the sea-buckthorn fruits, and soil of a sea-buckthorn orchard, and the characteristic of a colony is that the tube wall has no membranes and webs when the colony is cultured in a wort liquid medium; and the colony grown on a wort agar solid medium is white and butyrous, the surface is glossy, and the edge is orderly. A brand new strain is obtained after cultivation, screening and optimization, which can sufficiently adapt to the high acidity of the sea-buckthorns and overcome the defect that a port wine active dry yeast cannot adapt to the high acidity of the sea-buckthorns, and has high strain fermentation rate and superior safety; and the sea-buckthorn fruit wine produced by using the saccharomyces cerevisiae has the advantages of low residual sugar, high alcohol content, rich fruity aroma, plumpy wine body, and unique style of the sea-buckthorn fruit wine.

The invention relates to a preparation method of a biological organic and inorganic mixed liquid fertilizer. The preparation method comprises the steps of evenly mixing materials according to the ratio of EM solid glass Cassie to dried poultry litter to 1% EM stoste being (5-8) to (88-92) to (3-4) (parts by weight), yarding and fermenting for 21-25 days at room temperature, airing until the moisture content is lower than or equal to 30wt%; putting the materials into a fermentation tank according to the ratio of an ingredient to EM active carbon of 1 to 9 (weight ratio); hermetically fermenting for 5-7 days under an intermittent mixing condition; filtering the fermentation liquor to remove insoluble substance to obtain the clear liquid and store; mixing nutrients of nitrogen, phosphorus and potassium in the filtrate, so that the content ratio of nitrogen to phosphorus to potassium achieves the ratio of N to P2O5 to K2O being 1 to 2 to 2.5 (weight ratio), and N+P2O5+K2O is higher than or equal to 110g / L, and storing and bottling the materials. The preparation method disclosed by the invention is fast in fermentation speed, short in preparation period, and high in fermentation degree; the efficient biological organic and inorganic mixed liquid fertilizer can be obtained, and the preparation method has the effects of comprehensive nutrition, high fertilizer efficiency, nuisanceless property and obvious yield-increasing effect of crop.

The invention discloses a method for producing bio-organic fertilizers by quick fermentation of kitchen wastes. The bio-organic fertilizers are obtained by primary fermentation and secondary fermentation. Since primary fermentation products are partially returned to a fermentation matrix, fermentation rate is increased. According to characteristics of the kitchen wastes, yarrowia lipolytica, Saccharomyces willianus and bacillus laterosporus are selected for zymolysis of the kitchen wastes. Functional bacteria including streptomyces jingyangensis and the bacillus laterosporus are added, the streptomyces jingyangensis is capable of generating antibiotics to protect crops from pest and disease damages, and the bacillus laterosporus is resistant to high temperature, high salinity, dryness and high permeability and capable of improving soil salinity and alkalinity after being applied to soil and continuing decomposing macromolecular substances in the soil to provide nutrition for the crops, so that efficacies of the bio-organic fertilizers are improved.

The invention discloses a kitchen waste treatment and resourceful utilization method, and belongs to the technical field of solid waste treatment. The method includes the steps of modifying zeolite through the cooperation of magnesium salt, ferric salt and alkali liquor to obtain a material A, putting the material A into kitchen waste slurry for fermentation, and improving soil fertility with supernate of fermentation liquor as the denitrification carbon source and solid biogas residues as the good-performance nitrogen and phosphorus slow-release organic fertilizer. The method is easy to operate, the fermentation rate can be greatly increased, the rapid generation of organic acids with acetic acid and lactic acid as the main part is promoted, the organic acids serve as the denitrificationcarbon source, the efficient immobilized removal of nitrogen and phosphorus in the fermentation liquor can be realized, the defect that high-concentration nitrogen and phosphorus enter the denitrification carbon source is overcome, nitrogen and phosphorus can be transferred into the biogas residues to serve as the slow-release fertilizer, and the resourceful utilization of all kitchen waste is realized.

The invention provides a method for promoting growth of probiotics by adding rutin in a culture medium. According to the method, a probiotics strain (lactobacillus rhamnosus) is inoculated into a rutin-added improved MRS lactic acid bacteria culture medium for fermentation, wherein the improved MRS lactic acid bacteria culture medium includes the following components in percentage by weight: 2% of glucose, 2% of peptone, 1% of a beef extract, 0.5% of a yeast extract, 0.5% of sodium acetate, 0.2% of dipotassium phosphate, 0.2% of diammonium hydrogen citrate, 0.025% of magnesium sulfate, 0.005% of manganese sulfate, 0.3% of tween-80, and the pH is 7.0; 1g-5g of rutin (with the impurity of 99%) is added into each liter of the fermentation culture medium. According to the method, the fermentation speed of probiotics in fermentation liquid and the viable count of probiotics can be remarkably increased; after carrying out fermentation growth on probiotics for 24 hours, the maximal viable count of obtained probiotics fermentation liquid can reach about 10<9>CFU / mL; added rutin has the effects of decreasing capillary fragility, improving microcirculation, resisting radiation and free radicals and the like, and the functional effect of a cultured product can be substantially improved.

The invention relates to a colon bacillus alcohol fermentation engineering bacterium resisting alcohol. A colon bacillus mutant strain highly resisting the alcohol is obtained through a plurality of generations of directional screening; and an alcohol dehydrogenase gene II and a pyruvate decarboxylase gene of zymomonas mobilis are transferred into the mutant strain to obtain the novel colon bacillus alcohol fermentation engineering bacterium. When the engineering bacterium uses pentose and hexose as substrates to ferment for producing the alcohol, the engineering bacterium can still maintain higher fermentation rate under high-concentration alcohol, and has higher transformation rate of the alcohol.

The invention belongs to the technical field of domestic fungus production, relates to a domestic fungus culture material and especially relates to a mushroom medium and a preparation method thereof. The mushroom medium is prepared from 80-100 parts by weight of clay, 20-40 parts by weight of wood chip, 10-20 parts by weight of straw, 20-30 parts by weight of livestock and poultry faeces, 10-20 parts by weight of mushroom dreg, 2-5 parts by weight of lime and 6-12 parts by weight of a nutrition additive. The mushroom medium has the advantages of slow acid-base balance change, high content and long action time of nutrients in the nutrient additive, slow medium fermentation rate, long action time, long mushroom production period, and adaptability to different temperatures and humidities.

The fixing barm alcohol fermentation bio-system comprises: an air cleaning system, an adding system for fermented liquid, a fixing barm fluid bed fermentation reactor, a CO2 cycle system, and a collecting system for mash; all connected in turn with rubber pipe or metal pipe to connect out from last system top and connect in the next system bottom. This invention is simple, can improves material utility with low consumption and land area.

The invention relates to the field of wine making, and particularly relates to strains with high cellulase activity as well as a screening method and a use method of the strains. The strains with high cellulase activity are aspergillus. The screening method comprises the following steps of: A. enriching and cultivating bacteria through an inorganic salt culture medium; B. carrying out once or multiple-time inoculation on the bacteria enriched and cultivated on filter paper dipped with the inorganic salt culture medium; C. acquiring a cultured product at the fracture part of the filter paper, and carrying out once or multiple-time streaking on the cultured product on a carboxymethylcellulose culture medium plate, so as to obtain pure strains; D. cultivating the pure strains obtained from the step C in the inorganic salt culture medium and carrying out fibrinolysis on the cultivated pure strains; E. inoculating the pure strains with high fibrinolysis capacity in the step D to a steriled solid fermentation medium for cultivating; and F. extracting coarse enzyme liquid by adopting a water bath method, and carrying out enzyme activity test to obtain the strains with high cellulase activity. The strains and bread yeast are compounded for use and fermented by taking wine waste lees and brans as main materials. With the adoption of the strains with high cellulase activity, the fermentation rate of the wine waste lees can be improved.

The invention discloses pseudomonas mendocina and application thereof, and relates to pseudomonas mendocina NK-01 CCTCC M 208005, and a method for producing medium-and-long-chain-length polyhydroxyalkanoate which is the copolymer of 3-hydroxy hexanoate, 3-hydroxy octanoic acid, 3-hydroxy capric acid, Delta<7>-3-jalapinolic acid and 3-hydroxy stearic acid. In the method, Pseudomonas mendocina NK-01 CCTCC M 208005 is fermented in the culture medium of carbon source including glucose or treacle, or the mixture of glucose and treacle. The invention overwhelms the defects that long chain fatty acid needs to be added in the carbon source when long-chain-length polyhydroxyalkanoate, microbial growth is inhibited easily, the fermentation rate is low and the rate of production of fermentation products is low. By adopting the strain and the production process, a novel high elasticity biologic degradable material easy to be processed and shaped, namely the copolymer of 3-hydroxy hexanoate, 3-hydroxy octanoic acid, 3-hydroxy capric acid, Delta<7>-3-jalapinolic acid and 3-hydroxy stearic acid, can be produced. In addition, the fermentation conditions and the process enable large-scale industrial production.

The invention discloses a microbial additive for alfalfa haylage, a preparation method and an application thereof. The additive is prepared from a bacterial suspension of aspergillusoryzae and an MRS liquid nutrient medium for streptococcus lactis according to the ratio of 50-70%: 50-30% in weight percent, wherein per milliliter of the bacterial suspension of the aspergillusoryzae at least contains 1.0*108 aspergillusoryzae spores, and per milliliter of the MRS liquid nutrient medium at least contains 4.0*108 cfu streptococcus lactis. The microbial additive quickens the initial fermentation rate of lactobacillus, enhances acid production capability, effectively inhibits the activity of saccharomycetes and other undesirable microorganism, strengths haylage effect and effectively promotes the production performance of cows, beef cattle, mutton sheep and swine. The strain adopted by the invention can be obtained easily, can be produced massively on application and has practicability.

The invention relates to a preparation method for cactus-black rice nutritional vinegar. The preparation method comprises the steps of (1) raw material treatment: firstly processing the cactus, removing spines and peels from the cactus and pulping for use, then smashing the black rice, and carrying out starch gelatinization, liquidation and saccharification; (2) alcoholic fermentation: adding the cactus pulp into a saccharification fermented liquor and adding yeast to carry out alcoholic fermentation; and (3) liquid deep acetic fermentation and postprocessing processes such as rough filtration, sterilization, ageing, blending and fine filtration. Compared a conventional processing method for edible vinegar, the preparation method provided by the invention increases utilization rate and processing depth of the black rice and the cactus, is fast in fermentation speed, low in production cost and high in automatic degree, and is suitable for large-scale production. The cactus-black rice nutritional vinegar has pure fragrance, unique flavor, thick sour flavor and rich nutrition, can prevent hypertension, hyperlipidemia and hyperglycemia, relieve fatigue, resist aging, beautify looks and care skins, and greatly enhances the nutritional and health-care function of the edible vinegar.

The invention provides a method for improving the fermentation capability of a slurry dry process, belonging to the field of solid waste recycling, which can be used for solving the problems that reacted intermediate products and energy are difficult to transmit and diffuse in a medium resulted from the large viscosity of reaction matrix and high total solid (TS) concentration; slow hydrolysis acidification and low aerogenesis speed are caused by that a large amount of substances difficult to degrade exist in slurry and the further biotransformation is limited by the microbial residue cell-wall structure. In the method, dewatered slurry is placed in microwave of which the frequency is 915MHz and the power is 10-60kW for carrying out pretreatment for 3-10 minutes; the pretreated dewatered slurry is cooled to the selected fermentation temperature, and then mixes with seed slurry uniformly based on the mass ratio of 5: 1-3:1 to obtain a mixture which is placed in an anaerobic fermentation tank for standing for 3-5 days in a sealing and non-stirring state; a stirring motor is started, and the stirring speed is controlled to 10-30r / min; and materials are fed and discharged continuously so as to ensure the materials to stay in the anaerobic fermentation tank for 40-60 days. The method for improving the fermentation capability of the slurry dry process can be used to reduce the slurry viscosity and improve the dry fermentation rate and gas production rate; and in addition, the method for improving the fermentation capability of the slurry dry process can be used to save equipment investment and land occupancy compared with the wet fermentation.

The invention discloses an immobilization method of Aspergillus niger. According to the immobilization method, a pretreated fibrous material is used as an immobilization medium, the Aspergillus niger is activated and then cultured in a fermentation container containing the immobilized medium, and the strain is adsorbed on the fibrous material in the culturing process. When an airlift-type fiber bed reactor with polyester fiber as the immobilization medium is used for continuously producing citric acid for 15 batches, the acid producing level is not reduced and the fermentation process is stable. Ultimately, the citric acid yield is 88.65g / L, the fermentation rate is 1.06g / L / h, and the yield rate (citric acid yield / glucose consumption) is 93.3% (w / w). Compared with free fermentation, the yield of immobilized fermentation is obviously increased, the fermentation period is shortened, the number of mycelia in a fermentation broth is obviously reduced, and the viscosity is decreased therewith, so that separation of a product and the mycelia at a later period is facilitated.