The invention discloses a new preparation process of an agaricus bisporus culture material. The product belongs to the field of edible mushroom, and the invention is characterized by relating to the agaricus bisporus culture material. The invention aims at solving the problem that a new tunnel aeration fermentation method is provided to produce the agaricus bisporus culture material. The method has the advantages that: 1, wheat straw and chicken manure are taken as main raw materials, the raw materials are easily available, nitrogen content in the chicken manure is high, and heat productivityis high; 2, automatic mechanical continuous operation is adopted, manpower is saved, and mass production can be realized; 3, heat distribution in the culture material is uniform, fermentation temperature in the material is basically consistent, and quality can be guaranteed; and 4, fermentation speed is high, production efficiency is high, and less sundry bacteria are contained. The raw materialsused for producing the agaricus bisporus culture material comprise the wheat straw, the chicken manure, calcium carbonate, inorganic compound fertilizer, calcium superphosphate, lime powder and gypsum powder, and the product is used for planting agaricus bisporus.