The invention discloses a
saccharomyces cerevisiae strain with high yield of ester and low yield of higher
alcohol as well as a building method of the
saccharomyces cerevisiae strain, and belongs to the technical field of bioengineering. According to the building method provided by the invention, through completely knocking out an
amino acid transaminase gene BAT2 and an
ester hydrolase gene IAH1 in an original strain, and selecting a strong
promoter PGK1 over-expression
alcohol acetyltransferase I
gene ATF1 at the same time, the
saccharomyces cerevisiae strain with high yield of ester and low yield of higher
alcohol is obtained. Compared with a parent strain, other
fermentation performances of built recombinant
bacteria are not affected, the total quantity of
acetic acid ester is obviously increased and reaches 1303.6mg / L, wherein the content of
ethyl acetate is 52 times that of the original strain,
isoamyl acetate is increased to 73.7mg / L, the content of main higher alcohol is 151.8mg / L and is reduced by 61.4 percent in comparison with that of the original strain. By using the
saccharomyces cerevisiae, ester yield is significantly increased while the higher alcohol yield is reduced, the higher requirements of
white spirit related fields on
yeast are met and the application prospect is wide.