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1763 results about "Bio engineering" patented technology

Biological engineering, or bioengineering/bio-engineering, is the application of principles of biology and the tools of engineering to create usable, tangible, economically viable products.

System and method to bioengineer facial form in adults

A method and apparatus are provided for changing the form of the jaw and facial bones of an adult patient that did not develop fully during childhood. The method utilizes a device having a plate body with an expansion screw that fits within the mouth of the patient; flap springs that project from the plate body, and an overlay extending from the plate body. The device is placed within the mouth of the patient so that the overlay is in a position between at least two opposing teeth. In this position, opposing teeth contact the overlay during function (e.g. swallowing). This intermittent, unilateral application of force to the facial bones causes these bones to further develop, positioning out of place teeth into more proper positions, and inducing a more symmetrical and enhanced appearance of the face, as well as increasing the airway space behind the jaws. Concomitantly, the flap springs gently press against selected teeth that are out of alignment in order to guide those teeth into place. Simultaneously, the expansion device maintains these forces on the teeth, while assisting the jawbones to expand to accept the teeth in their proper position. The expansion device can be adjusted by small motors under the control of a microprocessor located on the body plate based on readings from sensors on the flap springs. The expansion device can be adjusted by remote signaling, using a global position satellite technology and global position coordinates.
Owner:BELFOR THEODORE +1

Silk fibroin fiber bundles for matrices in tissue engineering

The present invention provides a novel silk-fiber-based matrix having a wire-rope geometry for use in producing a ligament or tendon, particularly an anterior cruciate ligament, ex vivo for implantation into a recipient in need thereof. The invention further provides the novel silk-fiber-based matrix which is seeded with pluripotent cells that proliferate and differentiate on the matrix to form a ligament or tendon ex vivo. Also disclosed is a bioengineered ligament comprising the silk-fiber-based matrix seeded with pluripotent cells that proliferate and differentiate on the matrix to form the ligament or tendon. A method for producing a ligament or tendon ex vivo comprising the novel silk-fiber-based matrix is also disclosed.
Owner:ALLERGAN INC

Peptide for high performance inhibition of angiogenesis and method for preparing same and use thereof

ActiveCN1699408AImprove and enhance growthImprove and enhance the anti-tumor effectPeptide/protein ingredientsSkeletal disorderEscherichia coliInclusion bodies
The invention relates to a peptide for high performance inhibition of angiogenesis and method for preparing same and use, wherein high performance blood vessel production inhibiting agent RGD-ED with integration compatibility is designed, the inhibiting agent comprises polypeptide polypeptide-valine-arginine-arginine-alanine-aspartate-arginine-alanine-alanine-valine-praline, its one or two ends are connected with polypeptides containing arginine-glycine-aspartic acid sequence. The RGD-ED provided by the invention can be synthesized. The invention also discloses the expression of one RGD-ED in bacillus coli through gene engineering method, wherein the RGD-ED is prepared through the steps of inclusion body protein segregation, dissolution and renaturation, and ion-exchange chromatography segregation and purification.
Owner:CHINA PHARM UNIV

Programmed-release, nanostructured biological construct

A biologically engineered construct comprising of a polymeric biomatrix, designed with a nanophase texture, and a therapeutic agent for the purpose of tissue regeneration and / or controlled delivery of regenerative factors and therapeutic substances after it is implanted into tissues, vessels, or luminal structures within the body. The therapeutic agent may be a therapeutic substance or a biological agent, such as antibodies, ligands, or living cells. The nanophase construct is designed to maximize lumen size, promote tissue remodeling, and ultimately make the implant more biologically compatible. The nano-textured polymeric biomatrix may comprise one or more layers containing therapeutic substances and / or beneficial biological agents for the purpose of controlled, differential substance / drug delivery into the luminal and abluminal surfaces of the vessel or lumen, and the attraction of target molecules / cells that will regenerate functional tissue. The topographic and biocompatible features of this layered biological construct provides an optimal environment for tissue regeneration along with a programmed-release, drug delivery system to improve physiological tolerance of the implant, and to maximize the cellular survival, migration, and integration within the implanted tissues.
Owner:SCHAPIRA JAY N +1

Bioengineered tissue constructs and methods for production and use

Bioengineered constructs are formed from cultured cells induced to synthesize and secrete endogenously produced extracellular matrix components without the requirement of exogenous matrix components or network support or scaffold members. The bioengineered constructs of the invention can be treated in various ways such that the cells of the bioengineered constructs can be devitalized and / or removed without compromising the structural integrity of the constructs. Moreover, the bioengineered constructs of the invention can be used in conjunction with biocompatible / bioremodelable solutions that allow for various geometric configurations of the constructs.
Owner:ORGANOGENESIS

Succinic acid purification process through anaerobic fermentation

The invention belongs to the technical field of biological engineering and discloses a novel succinic acid purification process through anaerobic fermentation. By adopting the separation process based on solid-liquid separation, ultrafiltration, decoloration, acidification and crystallization and by introducing ammonium sulfate thermal cracking technology, the invention realizes cyclic utilization of the acid and the alkali, forms a closed clean production flow and effectively ensures the quality of the succinic acid used for food and medicine. The invention not only reduces the fermentation cost and improves the economic benefits but also reduces environment pollution, improves the ecological environment and promotes the sustainable development of the national economy. Therefore, the invention has wide social benefits.
Owner:NANJING UNIV OF TECH

Method of liquid fermentation culturing flammulina velutipes mycelium rich in Se and Zn

InactiveCN101228830AHigh content of active ingredientsHigh active ingredient content, added valueFungiFood preparationFood additiveBiotechnology
A method for cultivating golden mushroom mycelia abundant in Se and Zn elements through liquid fermentation belongs to the field of biotechnology. The invention substantially comprises cultivation of strains, cultivation of liquid seed, namely the domestication of two elements, and enrichment, fermentation and cultivation of two elements in a deep liquid, and collecting, drying and smashing of mycelia. The dried golden mushroom mycelia abundant in organic Se and organic Zn obtained with the method of the invention has over 35Mug / g organic Se and 4.25mg / g organic Zn. The powder of the mycelia with suitable food can be used as food additives and made into functional food, which has great health care function for the people in sub-health state resulted from deficient intake of microelements of Se and Zn. The invention is high in technological content, high content in effective components of the products, high additive value, which is suitable for industrial production.
Owner:JIANGNAN UNIV

Novel preparation method of peg-maleimide derivatives

The present invention relates to novel synthetic methods for PEG-maleimide derivatives, specifically, novel synthetic methods for PEG-maleimide. The final products thereof are useful as a pegylation reagent in the field of bioengineering and the medicinal field or the preparation of medical appliances and by conjugating with certain moieties or specific domains of polysaccharides and biological proteins such as antibodies as a bio-compatible or bio-degradable polymer.
Owner:SUN BIO INC

Programmed-release, nanostructured biological construct for stimulating cellular engraftment for tissue regeneration

A biologically engineered construct comprising of a polymeric biomatrix, designed with a nanophase texture, and a therapeutic agent for the purpose of tissue regeneration and / or controlled delivery of regenerative factors and therapeutic substances after it is implanted into tissues, vessels, or luminal structures within the body. The therapeutic agent may be a therapeutic substance or a biological agent, such as antibodies, ligands, or living cells. The nanophase construct is designed to maximize lumen size, promote tissue remodeling, and ultimately make the implant more biologically compatible. The nano-textured polymeric biomatrix may comprise one or more layers containing therapeutic substances and / or beneficial biological agents for the purpose of controlled, physiological, differential substance / drug delivery into the luminal and abluminal surfaces of the vessel or lumen, and the attraction of target molecules / cells that will regenerate functional tissue. The topographic and biocompatible features of this layered biological construct provides an optimal environment for tissue regeneration along with a programmed-release, drug delivery system to improve physiological tolerance of the implant, and to maximize the cellular survival, migration, and integration within the implanted tissues.
Owner:SCHAPIRA JAY N +1

Bacterial speices and method for producing succinic acid by microbial fermentation

This invention relates to a method for producing succinic acid type culture by animalcule fermentation, especially relating to an animalcule actinobacillus succinogenes SW0580 generating succinic acid by a fermentated sugariness raw material, its preservation number: CGMCC No. 1593 and a method for utilizing said animalcule fermentation to produce succinic acid, in which, said animalcule is the actinobacillus succinogenes separated from the rumen tested and 10-50g / L succinic acid can be produced by anaerobic fermentating 20-100g / L sugariness raw materials.
Owner:JIANGNAN UNIV

Saccharomyces cerevisiae and screening method and application thereof

ActiveCN101550400AShorten the fermentation cycleStrong low temperature fermentation abilityFungiBeer fermentationMicroorganismAlcohol
The invention relates to screening and application of saccharomyces cerevisiae suitable for the brewing of a series of kirschwasser, pertaining to the technical field of biological engineering. SC203 strain of the saccharomyces cerevisiae has been preserved in Center of General Microbiology of China Committee for Culture Collection of Microorganisms, with a registration number of CGMCC No. 2786. The strain is characterized by rapid growth, early fermentation, short fermentation cycle, strong tolerance against low temperature, acid, alcohol, sulfur dioxide and the like and is applicable to the brewing of such a series of kirschwasser as kirschwasser, ice kirschwasser, kirschwasser liqueur, kirschwasser ratafee and the like. In addition, the strain can also be used for the brewing of grape wine and other common ratafee and icewine.
Owner:山东九道生物科技有限公司

Method of producing cellulose, lignin and xylose by biomass material

A method for using biomass to produce cellulose, lignin and wood sugar comprises that breaking and screening the biomass, using low-boiling-point organic acid to immerge to decompose the biomass into wood sugar and oligosaccharide, and fluidize the cellulose and lignin, cools the liquid biomass after reaction, adds organic solvent, mixes uniformly and lays, after deposits completely, separating deposits and washes, dries to obtain non-shaped cellulose, distills the mother liquid of cellulose, recycles organic acid and generated volatile organics, distills mother liquid, cools and lays until complete deposition, separates deposits, washes, and dries to obtain wood sugar and oligosaccharide, evaporates to recycle the wood sugar and the organic solvent of deposited mother liquid, then separates the deposits, and dries to obtain lignin. The invention can completely circulate organic acid and organic solvent without waste discharged.
Owner:深圳市利世隆科技有限公司 +1

Cultivating material for edible fungus and its production technology

This invention involves a mushroom cultivation material and production process, it belongs to the field of bio-engineering technology. This mushroom cultivation material contains Chinese crud drug residue 30 ~ 40 share, cotton shells 50 ~ 60 share, urea 1 ~ 2 share, wheat bran 5 ~ 7 share, potassium sulfate 0.5 to 1.5 share, calcium superphosphate 0.5 to 1.2 share, zinc sulfate, magnesium sulfate, borax, ferrous sulfate together for 0.4~1 share, calcined lime 0.5 to1.3 share. Because of using materials containing substances such as Chinese crud drug residue, the produced mushroom cultivation material of this invention has antibacterial effect. Through the implementation of the invention reached so no longer waste residue cause environmental pollution, reduce the factory heavy economic burden caused by the sewage problem.
Owner:王贤

Process for coupling producing bioloigical diesel oil and 1,3-propylene glycol

InactiveCN1648207AReduce or even eliminate poison inactivationSave on separation costsOrganic chemistryLiquid hydrocarbon mixture productionBiodieselMicrobial transformation
The present invention belongs to the field of bioengineering technology, and especially a kind of couple producing biological diesel oil and 1, 3-propylene glycol. The present invention features that the course of lipase catalyzed reaction between methanol or ethanol and fat to produce biological diesel oil and the course of biologically converting glycerin into 1, 3-propylene glycol are coupled via membrane filtering for simultaneous proceeding. The present invention has the effects of eliminating the suppression of short-chain alcohol and glycerin on lipase, prolonging the service life of immobilized enzyme, omitting the glycerin separating and extracting step, lowering the production cost, saving time, raising production efficiency and providing economic and feasible process for producing biological diesel oil and 1, 3-propylene glycol.
Owner:DALIAN UNIV OF TECH

Method for producing fermented tea using microorganism

The invention provides a method for producing fermented tea by biological engineering method. The invention absorbs the traditional tea fermentation process, and fully employs the achievements of modern fermentation biological engineering research. Multiple fungus, microzyme and probiotic bacteria, which satisfy the relevant industry requirements and meet with the microbe categories for natural fermentation tea, compose the microbe combination; after water-removing and rolling, the fresh tea leaves enter biofermentation process without external water source so that the quality and security of products can be guaranteed. The invention has few process links and simple operation, greatly reduces the fermentation period and improves the production efficiency, implements the technical scale; the fermented tea has the same health-care function as the tea preserved for many years, and has good smell, lasting appeal, color, soup color and excellent taste.
Owner:胡徐玉 +1

Double aqueous phase extraction method for separating 1,3-dihydroxypropane from fermentation liquor

ActiveCN101012151ASolve the problem of low extraction efficiencySolve problems such as unrecyclableOrganic compound preparationHydroxy compound preparationInorganic saltsBio engineering
The invention discloses a double-water phase extracting technique of 1, 3-propanediol from ferment liquid in the biological engineering technical domain, which is characterized by the following: adding inorganic salt and hydrophilic organics in the ferment liquid of 1, 3-propanediol to form new double-water phase; extracting to separate 1, 3-propanediol from ferment liquid.
Owner:DALIAN UNIV OF TECH

Double aqueous phase extraction method for separating 2,3-dihydroxybutane from fermentation liquor

InactiveCN101012152ASolve the problem of low extraction efficiencySolve problems such as unrecyclableOrganic compound preparationHydroxy compound preparationInorganic saltsBio engineering
The invention discloses a double-water phase extracting technique of 2, 3-butanediol from ferment liquid in the biological engineering technical domain, which is characterized by the following: adding inorganic salt and hydrophilic organics in the ferment liquid of 2, 3-butanediol to form new double-water phase; extracting to separate 2, 3-butanediol from ferment liquid.
Owner:DALIAN UNIV OF TECH

Technique for preparing antimicrobial peptide by using Bacillus subtilis

The invention discloses a technique for preparing antimicrobial peptide by using Bacillus subtilis, belonging to the technical field of bioengineering. By using Bacillus subtilis BRT39 capable of generating broad-spectrum high-efficiency antimicrobial peptide as a production strain, liquid seed preparation, fermentation liquid preparation, fermentation liquid pretreatment, antimicrobial peptide extraction and refinement, antimicrobial peptide dry powder preparation and other techniques are carried out to prepare the high-purity antimicrobial peptide finished product, wherein the activity recovery rate is up to higher than 43.5%. The invention has the advantages of simple technique, high yield of antimicrobial peptide, and high purity; the prepared antimicrobial peptide dry powder belongs to a natural antimicrobial agent and has no toxic or side effect, and thus, can be used as a food preservative, feed additive, agricultural biological control agent or animal disease preventing agent; and the antimicrobial peptide has the advantages of no toxicity, no residue, no drug resistance and high safety.
Owner:XUZHOU UNIV OF TECH

Bioengineered tissue substitutes

In one aspect, the invention provides methods for forming a target tissue substitute. The methods of the invention comprise the following steps: (a) providing a scaffold comprising one or more layers of one or more arrays of microfibers, wherein one or more of the arrays of microfibers is designed to mimic the configuration of one or more structural elements in a target tissue; and (b) culturing cells on the scaffold to form a target tissue substitute. In another aspect, the invention provides implantable medical devices. The implantable medical devices of the invention comprise a scaffold comprising one or more layers of one or more arrays of microfibers, wherein one or more of the arrays of microfibers is arranged to mimic the configuration of one or more structural elements in a target tissue. Typically, cells are cultured on the scaffold to form a target tissue substitute.
Owner:UNIV OF WASHINGTON

Extracting and processing technique of carrageen

The invention relates to an extracting and processing technique of carrageen by utilizing eucheuma, which comprises the procedures of cleaning, alkali treatment, washing, enzymatic hydrolysis, extracting, filtering, potassic alteration, dehydration, crushing and drying. By adopting enzyme biotechnology technology, the extraction rate and quality of carrageenan are improved, while the production cycle and energy consumption thereof being lowered. Colloid dissolves out of carrageen cells completely after the organization of cellulose in carrageen cell wall being destroyed during the procedure of enzymatic hydrolysis, thus improving the extraction rate of carrageenan. Meanwhile, the time of boiling procedure is shortened and the temperature thereof is lowered. The yield of products is increased and steam consumption during the process is reduced. Small molecule pigments also dissolve out of carrageen cells during the procedure of enzymatic hydrolysis, thus improving the transparency and purity of the obtained carrageenan. Since alcohol is adopted in dehydration procedure, the water content of the products is greatly reduced. Meanwhile, bactericidal effect of alcohol is favorable to inhibit the breeding of microorganisms during the process, thereby fulfilling the objective of disinfection and bacteriostasis. The above technique has the advantages of high extraction efficiency, high utilization rate of raw materials and high transparency and purity of the obtained carrageenan.
Owner:青岛聚大洋藻业集团有限公司

Preparation method of biodegradable medicine composite macromolecular scaffold material

A preparation method of biodegradable medicine-compounded macromolecular scaffolding material includes the following steps: dissolving macromolecular polylactic acid, polycaprolactone and restenosis-resisting medicine in the solvent, pouring the prepared solution into a container for film-forming, making the said film into filament, dipping the said filament in the mixed solution prepared with L-lactic acid and diglycolide copolymer, solvent and restenosis-resisting medicine and drying in the air or freeze-drying, then soaking the said filament in anticoagulative solution, drying in the air, making filament wind round the mould, thermosetting and forming so as to obtain the invented product. The described solvent is chloroform, 1,4-dioxane and dimethyl sulfoxide, the restenosis-resisting medicine is taxol, taxadter, arotinoid ethylester, probucol, dectan and cilomosi, and the anticoagulative solution is prepared with carboxylated sulfurnic aid esterified chitin aqueos solution or heparin sodium aqueos solution and acetone through the process of mixing and solvation reaction.
Owner:TSINGHUA UNIV +1

Method for preparing antihypertensive peptide by utilizing combined enzyme enzymolysis silkworm chrysalis protein

The invention relates to the technical field of biological engineering, in particular to a method for preparing antihypertensive peptide by utilizing combined enzyme enzymolysis silkworm chrysalis protein. The method comprises the following steps: dissolving degreased dry silkworm chrysalis powder or silkworm chrysalis protein powder in water in the mass ratio of 1: 7 to 8, adjusting the pH value of the dissolved solution to be 7.5 to 8.5, and then adding combined protease to carry out enzymolysis reaction, wherein the content of alkaline protease is 0.3 to 0.5 percent of the degreased dry silkworm chrysalis powder or the silkworm chrysalis protein powder, the content of papain is 0.05 to 0.1 percent, the content of neutral protease is 0.05 to 0.1 percent, the enzymolysis reaction temperature is 55 to 65 DEG C, and the enzymolysis reaction time is 180 to 240 minutes. The prepared antihypertensive peptide has higher restriction effect on the ACE activity, antihypertensive function on hypertensive patients, does not influence normotensive patients, plays the better health care effect and is safe, has no poison or side effect and is low in cost.
Owner:常州康和生物技术有限公司

Saccharomyces cerevisiae strain with high yield of ester and low yield of higher alcohol as well as building and application of saccharomyces cerevisiae strain

PendingCN105385615AReduce outputOvercome flavor incongruityFungiHydrolasesEster hydrolaseBio engineering
The invention discloses a saccharomyces cerevisiae strain with high yield of ester and low yield of higher alcohol as well as a building method of the saccharomyces cerevisiae strain, and belongs to the technical field of bioengineering. According to the building method provided by the invention, through completely knocking out an amino acid transaminase gene BAT2 and an ester hydrolase gene IAH1 in an original strain, and selecting a strong promoter PGK1 over-expression alcohol acetyltransferase I gene ATF1 at the same time, the saccharomyces cerevisiae strain with high yield of ester and low yield of higher alcohol is obtained. Compared with a parent strain, other fermentation performances of built recombinant bacteria are not affected, the total quantity of acetic acid ester is obviously increased and reaches 1303.6mg / L, wherein the content of ethyl acetate is 52 times that of the original strain, isoamyl acetate is increased to 73.7mg / L, the content of main higher alcohol is 151.8mg / L and is reduced by 61.4 percent in comparison with that of the original strain. By using the saccharomyces cerevisiae, ester yield is significantly increased while the higher alcohol yield is reduced, the higher requirements of white spirit related fields on yeast are met and the application prospect is wide.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY

Biologically engineered stent

InactiveUS20100161032A1Promote formationPromote endothelial cell chemotaxisStentsOrganic active ingredientsMedicineIsograft
Biologically engineered stents are provided, some having novel double-walled and hybrid composition constructions that are suitable for multi-drug delivery. Some embodiments of biologically engineered stents (BES) in accordance with the invention can deliver drugs in the form of gene therapy vectors to cells in the walls of stented vessels, thereby promoting local production of therapeutic factors that attract and enhance the formation of endothelium in the stented vessel. Other embodiments of BES include xenografts, allografts or isografts comprising sleeve-like natural matrices derived from vessels of animal and human subjects including postmortem human donors.
Owner:AVELLANET FRANCISCO

Cropping GST protein thermal induction fusion expression plasmid and preparation method thereof

InactiveCN101215573ASuitable for western blot experimentsWill not affect judgmentVector-based foreign material introductionDNA/RNA fragmentationEscherichia coliProtein target
The invention belongs to the technical field of biological engineering and in particular is a heat-induced fusion expression recombinant plasmid of truncated GST protein and a process for preparation. The invention selects utility truncated GST188 protein which is composed of 188aa as a carrier, a pXXGST-1 recombinant plasmid which expresses short peptide fusion protein in an escherichia coli heat-induced expression system and a heat-induced type pXXGST-2 recombinant plasmid which is used to express comparison to realize the aim of synthesizing short peptide creatures, which is especially provided for scanning, drawing, positioning antigen linear epitopes and identifying epitope motif. Experiments of the invention prove the adaptability and the applicability of the GST188 core protein which is used as the short peptide creatures expression vector, in antigen epitope scanning identification, and in particular when anti- recombinant target protein antiserum is used to indentify the epitope motif.
Owner:SHANGHAI INST OF PLANNED PARENTHOOD RES +2

Cross-linking adsorbent resin with ultrahigh magnetic property and preparation method thereof

The invention discloses a magnetic ultrahigh cross-linking adsorbent resin and a preparation method thereof, belonging to the field of magnetic resin. The ultrahigh cross linking adsorbent resin provided by the invention is formed by a framework and magnetic particles wrapped in the framework, wherein the resin framework has the following basic structural formula that the saturation magnetization intensity of the resin is 0.1-10e mu / g; and the average particle size of the resin is 60-300 mu m. The resin provided by the invention is synthesized by adopting a suspension polymerization method and a reaction system comprises an oil phase, magnetic particles and a water phase, wherein the magnetic particles are wrapped in a double layer mode through a coupling agent and a dispersing agent, thereby enhancing the acid-resistant property and oil-phase dispersing property of the magnetic particles; chloromethyl styrene is adopted as a monomer to be synthesized into magnetic chlorine balls by adopting a one-step method; and then, a cross-linking reaction is carried out on the magnetic chlorine balls under the condition of nitrogen protection and in the presence of a buffer agent, thereby preparing the magnetic ultrahigh cross-linking adsorbent resin. The magnetic ultrahigh cross-linking adsorbent resin prepared by the invention can be applied to the elimination of organic pollutants in water, in particular to micro-pollution organic matters, and has wide application prospects in the fields of biological engineering, chemical analysis and the like.
Owner:NANJING UNIV

Method for preparing xylose and arabinose by hydrolyzing lignocellulose

The invention discloses a method for preparing xylose and arabinose by hydrolyzing lignocelluloses in the technical field of biological engineering. In the method, aqueous oxalate solution is used for carrying out catalytic hydrolysis on crushed lignocelluloses; lignocelluloses hydrolysate solution and xylose resides are obtained by solid-liquid separation after the reaction; the hydrolysate solution is neutralized to obtain oxalate sediment and obtain solid oxalate by solid-liquid separation again; the oxalate reacts with sulfate in an acidizing tank for regeneration to obtain the aqueous oxalate solution; the oxalate solution is used repeatedly to catalyze next batch of lignocelluloses raw material. The invention uses oxalic acid as organic acid catalyst and has the advantages of high conversion rate, strong selectivity, less side reaction, being capable of recycling, and the like, the oxalic acid can replace regular inorganic acid catalyst, and the invention solves the problem thatthe traditional technology has equipment erosion, evaporating equipment scaling, serious pollution and the like and can be used for hydrolyzing the hemicellulose in lignocelluloses raw material and used for preparing the xylose and the arabinose.
Owner:TSINGHUA UNIV
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