The invention discloses a culture medium used for cultivating bisporic mushrooms as well as the cultivating method thereof. Per 100 square meters of culture medium contain the following cultural materials: cotton seed hull 1800 to 3200 catties, waste cotton 0 to 1200 catties, corncobs 0 to 1200 catties, cottonseed meal 0 to 200 catties, cattle manure 2 to 4 cubic meters, chicken manure 0 to 1.2 cubic meters, urea 0 to 60 catties, quicklime 80 to 120 catties, plaster powder 0 to 120 catties, calcium superphosphate 0 to 120 catties, enzyme microorganisms 2 to 4 catties, and water of proper amount. The method includes the following steps: firstly, the cultural materials are mixed; secondly, the mixed cultural materials are composted and fermented; thirdly, the cultural materials are processed in a shed; fourthly, sowing is carried out; fifthly, the culture medium is distributed; sixthly, the soil is covered; seventhly, the mushrooms are obtained. The culture medium has low cost, the conglutination of the cultural materials is not easy to happen, and the cultural materials have easy pile-turning, thereby causing the composting and the fermentation to be laborsaving and timesaving; the enzyme microorganisms can not only speed up the fermentation of the cultural materials and improve the fermentation quality, but also facilitate the absorption and utilization of the culture medium by the mycelium of the bisporic mushrooms.