104 results about "Bacillus sp. ET" patented technology

The invention discloses a composite microorganism viable bacteria preparation, which contains pediococcus, yeast and bacillus with the total bacteria count of more than 1 billion per gram. The invention also discloses a method for preparing the composite microorganism viable bacteria preparation, which comprises the following steps: performing amplification liquid culture on various strains respectively according to a conventional method in the field; mixing the materials according to a required proportion; and performing solid fermentation, drying and pulverization to obtain the composite microorganism viable bacteria preparation. The invention also discloses application of the composite microorganism viable bacteria preparation in preparing an organic waste water purifying agent and an aquaculture water body purifying agent, a deodorizer, a degreasing agent or an agent for removing pipe blockages for the hygiene of a refuse tip, a public place, an agricultural product market or a kitchen, feed additives in poultry and animal husbandry or pet culture, and preparations for controlling agricultural microorganism diseases. The composite microorganism viable bacteria preparation can effectively decompose a large amount of putrescent organic matters, inhibit the growth of harmful microorganisms and eliminate peculiar smells, and has the characteristics of high ammonia nitrogen removal rate, no generation of residual sludge, effective deodorization, low cost and the like.

Fertilizer compositions for plant production are described, comprised of decontaminated manure and Bacillus spores, preferably a humic acid derived from lignite and, optionally, one or more of N compounds, P compounds, K compounds, and combinations of two or more of these compounds. Preferred compositions are those wherein the ingredients are blended into an admixture resulting in a granular product. Other preferred compositions are those blended into an admixture resulting in a powdered product. Preferably, the ingredients are formed into hardened prills or pellets. Processes for production and use are also presented.

The invention discloses a novel compound microorganism living bacteria agent. The agent comprises probiotics such as pediococcus, saccharomyces, bacillus and the like, wherein the total bacterial quantity is above 107 colony-forming units (cfu) / gram. The invention further discloses a method for preparing the novel compound microorganism living bacteria agent and applications of the novel compound microorganism living bacteria agent in preparation of organic sewage purification additives, deodorization fly dispelling agents, degreasing agents, pipeline blockage removing agents, feed additives and microorganism disease prevention agents. The novel compound microorganism living bacteria agent has the comprehensive effects of high removal rates to ammonia nitrogen, total nitrogen and total phosphorus, small sludge residues, energy saving, environmental friendliness and the like and the characteristics of convenience in use, safety and quickness in function, no secondary pollution, benefits to ecological protection, low treatment cost, good comprehensive profit and the like.

The invention relates a special organic culturing substrate capable of preventing and treating diseases for peppers and the preparation method of the special organic culturing substrate. The special organic culturing substrate is prepared from the following raw materials: furfural residues, edible fungus residues, oil residues, saw dust, a microbial fermentation fungicide EM composite fungicide, a trichoderma fungicide and a bacillus fungicide, wherein the bacteria liquid of the trichoderma fungicide has the effective bacteria content of 2X10<8> / g-5X10<8> / g, and the bacteria liquid of the bacillus fungicide has the effective bacteria content of 2X10<9> / g-4X10<9> / g. According to the invention, different organic wastes are compounded, are fermented, decomposed and sterilized, then the trichoderma fungicide and the bacillus fungicide which have the function of preventing and treating diseases of pepper roots are added, compositing is carried out, and finally, the special organic culturing substrate is obtained. The special organic culturing substrate prepared by the preparation method contains a large amount of active trichoderma, bacillus and the metabolic products of the active trichoderma and the bacillus, has an obvious inhibition effect on the diseases of the peppers, and improves the quality and the yield of the peppers.

The invention relates to a method for preparation of immobilized complexion bacterial agent used as controlling the secondary pollution to river bottom mud, which is characterized in that individually prepare photosynthetic bacterium fermenting culture liquid and bacillus fermenting culture liquid, and prepare immobilized complexion bacterial agent in solid fermenting culture medium. The immobilized complexion bacterial agent is not easy to leak after putting into the polluted river, not only exerting the degradation of microbial bacterial agent to contaminant, and also exerting the adsorption and immobilization of zeolitic powder to the contaminant given off by bottom mud, and thus gaining the ends of decreasing the bottom mud pollution to superjacent water. The tests indicate that the pollution deactivation can decreases dramatically with the immobilized complexion bacterial agent developed in the invention.

The invention relates to a mixed bacterial agent for reducing viscosity of thick oil, as well as a preparation method and a use thereof, belonging to the technical field of crude oil recovery. The mixed bacterial agent for reducing the viscosity of the thick oil comprises xanthomonas, pseudomonas, microbacterium and bacillus, and the volume ratio of fermentation solutions of all bacterial genera is (20-100):(40-180):(30-100):(10-80). The preparation method comprises the following steps: respectively fermenting and mixing first-stage seed solutions of all bacteria: (1) getting strains from sources: extracting the strains out of water of Bohai sea gulf, and taking thick crude oil as a carbon source for acclimation and screening; (2) preparing the first-stage seed solutions: respectively activating on a peptone-yeast slant culture medium and transferring into a liquid peptone-yeast culture medium for shaking and activation; and (3) preparing a fermentation solution of mixed bacteria: respectively transferring the first-stage seed solutions of the strains into a fermentation culture medium for culture, wherein the living bacteria count in the mixed bacteria is 10<8>-10<10> bacteria / ml. The mixed bacterial agent for reducing the viscosity of the thick oil is used for reducing the viscosity of the crude oil. The mixed bacterial agent for reducing the viscosity of the thick oil, disclosed by the invention, has the advantages of high activity, rapid growth, no secondary pollution, good viscosity-reducing effect and the like.

The invention provides a holothuria scabra artificial seeding breeding method. The method comprises the steps such as parent holothurian cultivating, induced spawning, spawn washing and hatching, larva cultivating, seedling collecting and childish holothurian cultivating, wherein spirulina powder, marine yeast, aquatic yeast and bacillus serve as bait, not only are bait sources convenient, but also the investment of single cell culture facilities is saved, meanwhile, manpower needed by single cell algae bait culture is saved, the holothurian seedling cost can be greatly saved, and the popularization of the holothuria scabra seedling breeding technology is facilitated. The substitutive bait is adopted to replace living single cell algae, invested funds of seeding facilities can be saved by about 25 percents, the human input of seeding can be saved by about 30 percents, and the ratio of the seeding input to the seeding output 1:3. Induced spawning is performed on the two days before or after the first day and the fifteenth day of a lunar month, the good effect of two times of spawn sampling in one month is achieved, the induced spawning success rate is 83.3 percents, and the technical problem that the induced spawning difficulty of holothuria scabra parent holothurian is high is effectively solved.

The invention belongs to the fields of agriculture, an agricultural technology and bacillus application, and relates to bacillus megaterium H3 and application thereof in promoting crop growth and reducing absorption of heavy metal by crops. The bacillus megaterium H3 is adopted as a raw material, and is subjected to bacteria activation so as to prepare into a bacterial suspension. The bacillus megaterium H3 bacterial suspension is inoculated in heavy metal contaminated soil, so that the rice growth can be promoted, and the cadmium content in rice grains is reduced. When a passivator and the bacillus megaterium H3 bacterial suspension are complex-used in the heavy metal contaminated soil, the vegetable growth can be promoted, the vegetable quality is improved, and the cadmium content of edible parts of vegetables is reduced.

The invention discloses an ecological remediation method, relating to raising seedling transplanting of an ecological remediation plant, and belonging to the technical field of plant cultivation. Vegetation is planted in a desert marginal area mixed with desert alga and bacillus, thereby playing wind prevention and sand fixation effects; with the adoption of the method, the land is protected compared with a single plant method, thereby playing the wind prevention and sand fixation effects; as seeds are strong in fertility, strong in adaptability and strong in stress resistance, the use amount of pesticides and fertilizers is reduced, and the liquorice quality is improved; and the algae and microorganism are united to be used, the mutual effect of the algae and the microorganism promotes growth, therefore, with the adoption of the method, a sand stabilizing structure is formed in desert soil rapidly and permanently compared with a former ecological restoration technology in the prior art, and the benefit of ecological environment is generated.

The invention provides a crop straw decomposing agent produced by using seaweed powder to absorb a composite microbial fermentation stock solution and a preparation method thereof. The active components of the composite flora in the composite microbial fermentation stock solution include: photosynthetic bacteria, yeast, acetic acid bacteria, actinomycetes and Bacillus, the total number of strains is 0.1-1 billion / ml. After using the seaweed powder to absorb the compound bacteria and microbial fermentation stock solution, it is dehydrated by a filter press and dried at low temperature. Finally, it is made into a large amount of bacterial flora, organic acids, organic matter and trace amounts. Elemental decomposing agent for organic materials, the decomposing agent effectively increases the organic matter content of the soil, and is an ideal soil remediation agent and microbial balance agent.

The invention relates to a weathered coal bio-organic fertilizer and a production method thereof. The weathered coal bio-organic fertilizer is characterized by comprising the following raw materials in part by weight: 45 to 55 parts of weathered coal, 20 to 30 parts of chilli powder residue, 8 to 12 parts of cottonseed cake, 10 to 15 parts of saw powder or straw, 1 to 2 parts of urea, 50 to 60 parts of water, 8 to 12 parts of trichoderma culture solution and 8 to 12 parts of bacillus culture solution. The production method is characterized by comprising the following steps of: adding the weathered coal, the chilli powder residue, the cottonseed cake, the saw powder or straw and the water into a fermenting tank; adding the urea and the trichoderma culture solution into the fermenting tank to perform fermentation; and then adding the bacillus culture solution into the fermenting tank with stirring to continuously perform fermentation to obtain the weathered coal bio-organic fertilizer. The weathered coal bio-organic fertilizer has the advantages of wide source of the raw materials, simple method, high bioactivity, comprehensive nourishment supplying to crops, robust growth, high disease resistance and stress resistance, capacity of effectively improving the utilization ratio of an organic fertilizer and a chemical fertilizer, increment in production and income, improvement on soil, rich nutrition and no chemical residues.

The invention relates to a special biological disease-resistant vermin-proof culture medium for flowers and a preparation method thereof. The special biological disease-resistant vermin-proof culture medium for flowers is prepared from smoke dross, furfural residue, microbial fermentation inoculum, superphosphate, trichoderma microbial inoculum, bacillus microbial inoculum and expanded perlite. The preparation method comprises the following steps: compounding smoke dross, furfural residue, microbial fermentation inoculum and superphosphate; after the mixture is decomposed, sterilizing, adding trichoderma microbial inoculum and bacillus microbial inoculum with disease-resistant function; and after composting for some time, adding the expanded perlite. Abundant active trichoderma and bacillus and metabolites thereof contained in the culture medium provided by the invention have obvious inhibiting action on flower diseases; and the smoke residue and resolvents thereof have the action of expelling and killing vermins which are easily generated in the medium. When being used for planting flowers, the special culture medium for flowers can protect the environment, and is beneficial to human health.

A nutritive bio-additive for feed of livestock and fowls contains fermented product of lysine, bacillus and photosynthetic bacteria, bean cake, plant starch and trace elements. Its advantages are high biological effect-cost ratio, high effect on decomposing harmful substances in animal body, and rich methionine and somatic protein for high immunity.

The present invention relates to microbiology technology, and is especially microbial preparation for repairing aniline contaminated water and its preparation process and usage. The microbial preparation consists of aerobes Pseudomonas, Alealigenes, Zoogloea, Aeromonas, Arthrobacter and Bacillus in the weight ratio of 8-10 to 3-4 to 1-2 to 1-3 to 1-2 to 1-2, as well as culture liquid containing aniline. It is applied into aniline contaminated water to reach aniline degrading rate up to 80-100 %. It is prepared through domestication in relatively high concentration nitro arene compound and nutritious compound and screening. It has high growth and reproduction capacity, powerful adaptability, high biomass, low cost, powerful water purifying effect and no secondary pollution.

The invention relates to a high-efficiency extraction method of cannabidiol, and belongs to the technical field of extraction methods of plant effective components. In order to solve the problems of low extraction efficiency, low product purity and low yield of an existing cannabidiol extraction method, the invention provides the high-efficiency extraction method of cannabidiol. The method comprises the following steps: fermentating a hemp raw material by trichoderma harzianum, carrying out ultrahigh-pressure treatment, carrying out fermentation by bacillus polymyxa, carrying out washing and centrifugation, removing impurities by macroporous adsorption resin, and carrying out purification by a silica gel chromatography column, so that a cannabidiol solid with purity of 98% is finally obtained, and the tetrahydrocannabinol impurity is not detected. When cannabidiol is extracted from hemp by using the method, a pretreatment method combining fungus solid fermentation, ultrahigh pressure treatment and bacillus solid fermentation is adopted, an enzyme system generated by microbial fermentation metabolism can destroy the dense physical and chemical structure in hemp cells, and the hemp cells are thoroughly crushed by ultrahigh-pressure treatment, so that cannabidiol is fully released, and the extraction rate of cannabidiol and the utilization rate of hemp can be remarkably improved.

The invention discloses a protein feed and a preparation method of the protein feed. The preparation method comprises the following steps of: mixing bean pulp, water, rice bran and bran according to a weight proportion of 5-7:5-7:1:1, and obtaining a base material; mixing lactobacillus plantarum, saccharomyces cerevisiae and bacillus according to a weight proportion of 0.9-1.1:0.9-1.1:1, and obtaining a bacterial solution with the total bacterium number more than 109 CFU / ml; and preparing a fermentation substance by 100 parts of base material, 10-20 parts of white popinac leaf, 7.5-15 parts of bacterial solution and 5-6 parts of brown sugar or white sugar, or 100 parts of base material and 20-50 parts of white popinac leaf, wherein when more than 20 parts of white popinac leaf is added, 0.05-0.08 part of 0.4 wt% ferric sulfate solution, 7.5-15 parts of bacterial solution and 5-6 parts of brown sugar or white sugar are added at every five parts of white popinac leaf. The protein feed is high in protein content, low in mimosine content, simple in technology, low in cost and pollution-free.

A method for producing a lactic acid bacteria fermented milk product comprising fermenting milk with both inoculated lactic acid bacteria (LAB) and inoculated Bacillus bacteria.

The invention provides a composite microbial disease / insect-resistant agent for green vegetables and a manufacturing method thereof. The composite microbial disease / insect-resistant agent for green vegetables comprises a composite microbial inoculant and a fertilizer, wherein the composite microbial inoculant is a mixed microbial inoculant formed by fermenting Bacillus and Rhodospirillum in a culture medium; the fertilizer comprises a potassium fertilizer and a pH regulator; the mass ratio of the composite microbial inoculant to the fertilizer is (30-50):(50-70); and the mass ratio of the Rhodospirillum microbial inoculant to the Bacillus microbial inoculant is (1-3):1. By adopting the composite microbial inoculant, the agent has the advantages of sufficient nutrients and reasonable supply, can enhance the nutrient absorption capacity of the plant root, and effectively inhibits harmful bacteria from growth and reproduction. When being used for a long time, the agent can improve the soil and avoid multiple diseases and insects due to deficiency of nutrient elements.

The invention relates to a main material for constructing a tillage layer of newly rectified cultivated land, a construction method of the tillage layer and application. The main material for constructing the tillage layer of the newly rectified cultivated land comprises peat materials, active organic materials and biological activators; the peat materials include herbal peat, woody peat and / or humus soil; the active organic materials are natural plant organic materials rich in polysaccharide / organic acid / amino sugar; the biological activators comprise amino acid bacteria and bacillus which promote degradation of all the active organic materials to form soil organic matter, facilitate formation of a good agglomeration structure of the soil and activate rapid circulation of the system. Thetillage layer is rapidly constructed by engineering, a traditional mode is subverted, a lengthy cultivation and ripening process formed by the high-quality tillage layer can be simplified into one-time engineering operation, geotechnical cultivation of the newly rectified cultivated land is improved in a short time, and crop yields and benefits are continuously ensured.

A strain of Lactobacillus salivarius isolated from resected and washed human gastrointestinal tract inhibits a broad range of Gram positive and Gram negative microorganisms and secretes a product having antimicrobial activity into a cell-free supernatant. The activity is produced only by growing cells and is destroyed by proteinase K and pronase E, the inhibitory properties of the strain and its secretory products being maintained in the presence of physiological concentrations of human bile and human gastric juice. The strain exhibits a broad-spectrum of activity against bacteria including Listeria, Staphylococcus, including methocillin resistant St. aureus (MRSA), and Bacillus, but does not inhibit many closely related lactobacilli. An antimicrobial agent is obtained from the strain which has bacteriocin-like properties.

The invention provides a method for labeling and culturing a penaeus japonicus family in the same pond of a higher-place pond and relates to the cultivation of young shrimps. Sand is paved in the higher-place pond and the higher-place pond is disinfected; a pipeline device for inflating and oxygenating is mounted, and seawater is filtered by the paved sand; the whole pond is disinfected, and a nutrition preparation and a bacillus viable bacterium preparation are sprayed; a fixed suspension net box is produced; the young shrimps are selected and transported; after the young shrimps are put into the pond, the water level is raised; the bacillus viable bacterium preparation and an organic fertilizer are sprayed, and povidone iodine is sprayed to disinfect a water body; after the young shrimps are put into the pond, fairy shrimp larvae which are manually incubated are fed in the morning and at night of each day within first 10 days; during a cultivation post-period, a young shrimp particle feed added with vitamins, amino acids and a lactobacillus viable bacterium preparation is fed; during the cultivation post-period, a water sample is taken every 5 days to observe the quantity of vibrio; when a lot of the vibrio is bred, a disinfectant is used for disinfecting the higher-place pond and antibiotics are added into a bait; when the young shrimps grow to be about 4cm, a fluorescent mark is injected to carry out family marking, and all the marked families are put into the same higher-place pond to be poly-cultured, and then the same pond property testing experiment is carried out.

The invention discloses a bactericidal composition containing bacillus amyloliquefaciens and difenoconazole, and application of the bactericidal composition. The bactericidal composition is prepared by taking the bacillus amyloliquefaciens and the difenoconazole as active components, wherein the mass ratio of the bacillus amyloliquefaciens to the difenoconazole is equal to (5 to1)-(1 to 5). The invention also discloses preparation methods of agricultural preparation forms, i.e., wettable powder and a suspending agent, of the bactericidal composition, and application of the agricultural preparation forms in control of strawberry anthracnose, powdery mildew and gray mold. Compared with a single agent, the bactericidal composition provided by the invention has an obvious synergistic effect for the strawberry anthracnose, the powdery mildew and the gray mold, and can remarkably improve prevention and control effects; the potential drug resistance risk of the strawberry anthracnose, the powdery mildew and the gray mold for the difenoconazole and the bacillus is reduced; after the bactericidal composition is used, the consumption of chemical pesticide is reduced, the pesticide cost is lowered, and the problems of environmental pollution and residue of agricultural products are effectively solved; the bactericidal composition is safe to humans and animals, good for organisms and environmentally safe, thus being widely applied to the control of the strawberry anthracnose, the powdery mildew and the gray mold.

This invention describes the discovery and synthesis of N-thiolated 2-oxazolidinones as a new class of anti bacterial agents. These compounds can be synthesized from 2-oxazolidinones by Ndeprotection and N-sulfenylation. These new substances were found to exhibit potent anti-bacterial activity, including bacteriostatic properties against Staphylococcus spp., including methicillin resistant Staphylcoccus aureus (MRSA), and Bacillus spp., including Bacillus anthracis.

The invention relates to a feedstuff additive prepared from bacillus antibacterial peptides and bacillus probiotics and a using method thereof in feedstuff, and belongs to the field of feedstuff additives. The compound feedstuff additive is prepared from bacillus antibacterial peptides and bacillus probiotics, wherein the content of bacillus antibacterial peptides in per gram of compound feedstuff additive is 2-20 mg, and the content of bacillus probiotics in per gram of compound feedstuff additive is 1.0-5.0*109 cfu. The feedstuff additive can be used as an antibiotic substituted product, antibiotic residues are removed, the quality of meat, eggs and milk is improved, and the feedstuff additive can be used for preventing and treating kinds of animal diarrhea, improving production performance of animals, immunity and disease resistance and increasing the feedstuff conversion rate. The additive is a green feedstuff additive and is beneficial to improving the quality of livestock and poultry products and health of humans.

The invention discloses a strawberry growing technology. Through years of growing experience, the proportion of nitrogen to phosphorus to potassium in each growing process is matched reasonably; during fertilization, a thin-fertilizer frequent-fertilization method is adopted, so that each nutrient element can be supplemented in time in each growing process of strawberries, and the grown strawberries are bright in color and luster, uniform in shape and fine in mouthfeel; specific to the problem of year-by-year production reduction troubling farmer households, a rare earth micro fertilizer is applied additionally at a fruiting stop period, and inorganic phosphorus potassium in soil is decomposed through potassium bacteria and bacillus to increase the permeability of soil, so that the strawberry yield of the next year will not be lowered.

The invention relates to fermentation of aquatic feed and especially relates to a treatment method for improving the digestion and utilization ratio of broad beans of fish. The treatment method comprises the following steps: (1) preparation of a compound microbial inoculum: separately inoculating YPD liquid nutrient media with yeast and bacillus to obtain a yeast fermentation solution and a bacillus fermentation solution, and then mixing the yeast fermentation solution and the bacillus fermentation solution according to a volume ratio of (1:1) to (1:1.2) to obtain the compound microbial inoculum; (2) preparation of broad beans: soaking dry broad beans in salt water at room temperature for 12-24h, and performing boiling, filtering and cooling; and (3) solid state fermentation of the broad beans: mixing the broad beans and the compound microbial inoculum according to a ratio of 1kg of broad beans to 400-450ml of compound microbial inoculum, performing uniform stirring, and maintaining the temperature at 30-32 DEG C for 20-24h to obtain the broad bean fermented material. The invention provides the method based on fermentation of the broad beans by using the compound microbial inoculum, and proven by experiments, the method can reduce anti-nutritional factors in the broad beans and improve the digestion and utilization ratio of the broad beans of the fish and the growth performanceof the fish.

The invention relates to a novel biological floc. The biological floc includes microalgae aggregates and commensalism heterotrophic bacteria, and the commensalism heterotrophic bacteria are Pesudomonas sp. and Bacillus sp. The biological floc significantly improves the water quality of the nursing pond of the litopenaeus vannamei, improves the survival rate, growth index and immunity of the litopenaeus vannamei, reduces the application amount of compound feed and external carbon sources, and reduces the aeration intensity and quantity of exchanged water.