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Composite microorganism viable bacteria preparation and preparation method and application of same

A technology of compound microorganisms and live bacteria preparations, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high cost, large amount of excess sludge, large odor, etc., and achieve effective deodorization costs. , High ammonia nitrogen removal rate, and the effect of eliminating odor

Inactive Publication Date: 2009-09-23
SHANGHAI ECO WELL BIOSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a method that can avoid the above-mentioned defects, effectively decompose organic matter, and inhibit harmful microorganisms in order to overcome the shortcomings of the existing technology for treating organic sewage with large amount of residual sludge, large odor and high cost. Growth, deodorizing compound microbial living bacteria preparation and its preparation method and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Carry out amplified culture respectively:

[0026] After activation of Pediococcus cerevisiae CGMCC 1.123 on a slant, inoculate it in a liquid medium and cultivate it at 35°C for 48 hours. The liquid medium formula: 3% glucose, 1.5% tryptone, 3% beef extract, 2% peptone, Tween 80 0.1%, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.25%, pH6.5.

[0027] After Pichia pastoris CGMCC 2.1463 is activated on a slant, it is inoculated in a liquid medium and cultured at 28°C for 48 hours. The liquid medium formula: 1% glucose, 0.2% malt powder, 0.2% yeast extract, 0.5% sodium acetate, pH7 .0.

[0028] After the Bacillus amyloliquefaciens CGMCC 1.1099 was activated on a slant, it was inoculated in a liquid medium and cultured at 28°C for 48 hours. The liquid medium formula: 1.5% soybean powder, 1% starch, 0.2% yeast extract, 1% glucose, pH6. 5.

[0029] Viable bacteria count, detect the number of bacteria of Pediococcus cerevisia...

Embodiment 2

[0032] (1) Carry out amplified culture respectively:

[0033] After the Pediococcus lactis ATCC 12697 was activated on the slant, it was inoculated in a liquid medium and cultured at 33°C for 48 hours. The liquid medium formula: 3% glucose, 1.5% tryptone, 3% beef extract, 2% peptone, Tween 800.1 %, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.25%, pH6.5.

[0034] Candida albicans CGMCC 2.538 and Saccharomyces cerevisiae CGMCC 2.769 were activated on a slope, inoculated in a liquid medium, and cultured at 27°C for 48 hours. The liquid medium formula: 1% glucose, 0.2% malt powder, 0.2% yeast extract, acetic acid Sodium 0.5%, pH 7.0.

[0035] After the Bacillus subtilis CGMCC 1.1414 is activated on the slant, it is inoculated in the liquid medium and cultured at 27°C for 48 hours. The liquid medium formula: 1.5% soybean powder, 1% starch, 0.2% yeast extract, 1% glucose, pH6.5 .

[0036] Viable count, detecting the number of Pediococ...

Embodiment 3

[0039] (1) Carry out amplified culture respectively:

[0040] Pediococcus pentosaceus ATCC 25744 was activated on a slant, inoculated in a liquid medium, and cultured at 34°C for 44 hours. The liquid medium formula: 3% glucose, 1.5% tryptone, 3% beef extract, 2% peptone, Tween 800.1%, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.25%, pH6.5.

[0041] Saccharomyces Dekkeri ATCC 10559 was activated on a slant, inoculated in a liquid medium, and cultured at 26°C for 52 hours. The liquid medium formula: 1% glucose, 0.2% malt powder, 0.2% yeast extract, 0.5% sodium acetate, pH7 .0.

[0042] After the Bacillus licheniformis CGMCC 1.521 was activated on the slant, it was inoculated in the liquid medium and cultured at 26°C for 52 hours. The liquid medium formula: 1.5% soybean powder, 1% starch, 0.2% yeast extract, 1% glucose, pH6.5 .

[0043] Viable count, detection of the number of Pediococcus pentosaceae, Saccharomyces dekkerae, and Ba...

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Abstract

The invention discloses a composite microorganism viable bacteria preparation, which contains pediococcus, yeast and bacillus with the total bacteria count of more than 1 billion per gram. The invention also discloses a method for preparing the composite microorganism viable bacteria preparation, which comprises the following steps: performing amplification liquid culture on various strains respectively according to a conventional method in the field; mixing the materials according to a required proportion; and performing solid fermentation, drying and pulverization to obtain the composite microorganism viable bacteria preparation. The invention also discloses application of the composite microorganism viable bacteria preparation in preparing an organic waste water purifying agent and an aquaculture water body purifying agent, a deodorizer, a degreasing agent or an agent for removing pipe blockages for the hygiene of a refuse tip, a public place, an agricultural product market or a kitchen, feed additives in poultry and animal husbandry or pet culture, and preparations for controlling agricultural microorganism diseases. The composite microorganism viable bacteria preparation can effectively decompose a large amount of putrescent organic matters, inhibit the growth of harmful microorganisms and eliminate peculiar smells, and has the characteristics of high ammonia nitrogen removal rate, no generation of residual sludge, effective deodorization, low cost and the like.

Description

technical field [0001] The invention relates to a compound microbial live bacteria preparation and its preparation method and application. Background technique [0002] At present, the following methods are usually used in China to treat organic sewage such as urban domestic sewage, food industry wastewater, and animal manure sewage: when the COD is greater than 1000mg / L, an anaerobic and then aerobic biological treatment process is adopted; when the COD is less than 1000mg / L, the aerobic biological treatment process (activated sludge method, biofilm method) is adopted. After the treatment by the above method, the general COD can reach the standard, but the treatment effect of ammonia nitrogen is poor. Among them, the activated sludge method has disadvantages such as a large amount of excess sludge, a large odor in sludge treatment, and high treatment costs. Since the 1990s, foreign countries began to extract high-efficiency degrading bacteria from polluted soil for organ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34A61L9/00C11D3/38A23K1/16A01P3/00C12R1/01C12R1/645C12R1/725C12R1/865C12R1/84C12R1/07C02F103/20C02F103/32A23K10/18
Inventor 张琴陈建吕曾伟明胡海峰
Owner SHANGHAI ECO WELL BIOSCI
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