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311 results about "Tryptone" patented technology
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Tryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin. Tryptone is commonly used in microbiology to produce lysogeny broth (LB) for the growth of E. coli and other microorganisms. It provides a source of amino acids for the growing bacteria. Tryptone is similar to casamino acids, both being digests of casein, but casamino acids can be produced by acid hydrolysis and typically only have free amino acids and few peptide chains. Casamino acids are similar to tryptone, the latter differing by being an incomplete enzymatic hydrolysis with some oligopeptides present, while casamino acids are predominantly free amino acids.
Method for breeding test-tube plantlets of Paphiopedilum henryanum Braem seeds
InactiveCN101558744AEffective cultivation methodPlant tissue cultureHorticulture methodsActivated carbonBiotechnology
The invention discloses a method for breeding test-tube plantlets of Paphiopedilum henryanum Braem seeds, comprising seed bourgeoning, proliferation and differentiation, and sound seedling rootage. A culture medium for the seed bourgeoning includes 1 / 4 of MS, 15 percent (in volume) of coconut milk, 20 g / L of sucrose, 1 g / L of activated carbon and 0.6 percent of agar, with a pH of 5.2-5.4; a culture medium for the proliferation and differentiation comprises 1 / 2 of MS, 0.5-2.0 mg / L of 6-BA, 0.2 mg / L of NAA, 2 g / L of tryptone, 15 percent (V / V) of coconut milk, 20 g / L of sucrose, 1 g / L of activated carbon and 0.6 percent of agar, with a pH of 5.2-5.4; and a culture medium for the sound seedling rootage comprises 1 / 2 of MS, 1.0 mg / L of NAA, 15 percent (in volume) of coconut milk, 20 g / L of sucrose, 1 g / L of activated carbon and 0.6 percent of agar, with a pH of 5.2-5.4. The method achieves over 45 percent of seed bourgeoning rate and over 90 percent of planting rate and transplantation survival rate.
Owner:SUBTROPICAL CROPS INST OF GUIZHOU PROVINCE
Low serum efficiency mycoplasma gallisepticam attenuated strain culture medium and preparation method thereof
ActiveCN103074246AIncrease the titer of live bacteriaReduce allergic reactionsBacteriaMicroorganism based processesAntigenCulture mediums
The present invention relates to a low serum efficiency mycoplasma gallisepticam attenuated strain culture medium and a preparation method thereof, and belongs to the technical field of veterinary biology. The culture medium comprises: (1) a base culture medium; and (2) an auxiliary culture medium, wherein the auxiliary culture medium mainly comprises MEM, yeast extract powder, tryptone, glucose, an inorganic salt, and the like, and growth, high titer and stability of the semi-finished product can be ensured with the auxiliary culture medium. According to the present invention, a titer of the semi-finished product bacterial liquid prepared by using the preparation method is up to 10<11> CCU / ml; and the culture medium adopts reduced pig serum to culture mycoplasma gallisepticam so as to reduce allergic stress reactions on chicken by heterologous pig serum, consider animal biosafety, improve antigen titer, and reduce production cost.
Owner:兆丰华生物科技(南京)有限公司
Culture medium for composite enrichment of salmonella, Listeria monocytogenes and Staphylococcus aureus, and preparation thereof
InactiveCN101412978AShorten enrichment timeBacteriaMicrobiological testing/measurementLithium chlorideStaphylococcus cohnii
The invention discloses a complex enrichment medium used for salmonella, listeria monocytogenes and staphylococcus aureus and a method for preparing the same. The complex enrichment medium comprises the following components in weight portion: 15 to 19 portions of tryptone, 2 to 4 portions of peptone, 2 to 3 portions of sodium dihydrogen phosphate, 2 to 3 portions of glucose, 0.5 to 1.5 portions of aesculin, 1, 000 portions of distilled water, 1 to 2.5 portions of sodium pyruvate, 3 to 10.0 portions of mannitol, 1.0 to 25.0 portions of sodium chloride, 1 to 2.5 portions of lithium chloride, 0.0001 to 0.0002 portion of potassium tellurite, and 0.005-0.015 portion of nalidixic acid, wherein the pH value is between 7.1 and 7.5. The complex enrichment medium can inhibit the growth of other pathogenic microorganisms while performing enrichment on three target pathogens, thus the complex enrichment medium can be directly applied to the isolation culture and the biological assay test of target bacteria, and can also be directly applied to a detection technology of a plurality of pathogens based on one detection platform for multiple PCR and the like to make a diagnostic report.
Owner:SOUTH CHINA UNIV OF TECH
Porcine reproductive and respiratory syndrome live vaccine heat-resistant freeze-drying protective agent and preparation method thereof
ActiveCN102166362AReduce loss rateHigh degree of aging resistanceViral antigen ingredientsInorganic non-active ingredientsLoss rateFreeze-drying
The invention relates to a porcine reproductive and respiratory syndrome live vaccine heat-resistant freeze-drying protective agent and a preparation method thereof. The heat-resistant freeze-drying protective agent is mixed by the raw materials based on weight percentage: 1-2% of gelatin, 4-6% of trehalose, 1-2% of tryptone, 1-2% of trypticase, 1-2% of polyvinylpyrrolidone, 0.164% of dipotassium phosphate, 0.052% of monopotassium phosphate, 1% of sorbic alcohol, 1% of sodium glutamate, 1% of arginine salt and the balance of water. The preparation method comprises the steps of: preparing the materials into two mixed solution, respectively preparing in a mixing way by means of degerming to prepare a heat-resistant freeze-drying protective agent, mixing according to the proportion of the protective agent to the virus-resistant raw material as 1:1, performing split charging, and drying in a freezing way. Before drying in a freezing way, the virus loss rate is low, a freeze-drying product is high in ageing-resistant degree, and the virus is invariable in content after the freeze-drying product is preserved at the temperature of 2-8 DGE C for 24 months. After the invention is used, the technical neck bottle that the porcine reproductive and respiratory syndrome live vaccine can be preserved and transported under the condition of -15 DEG C is solved.
Owner:WUHAN CHOPPER BIOLOGY
Clear liquid fermentation medium for clostridium butyricum and fermentation culture method thereof
InactiveCN102363754AHigh effective contentSimple post-processingBacteriaMicroorganism based processesBiotechnologySodium bicarbonate
The invention relates to a clear liquid fermentation medium for clostridium butyricum. The clear liquid fermentation medium, the pH value of which is 7-8, is made from glucose, tryptone, a yeast extract powder, ammonium sulfate, sodium bicarbonate, a maize liquid powder and the like. The clear liquid fermentation medium for clostridium butyricum contains metal salts for promoting the growth of gemma, wherein the metal salts are manganese sulfate, magnesium sulfate and ferrous sulphate. The enrichment medium of the clear liquid fermentation medium for clostridium butyricum contains yeast extract, beef extract, tryptone, glucose, soluble starch, sodium chloride, sodium acetate trihydrate, cysteine hydrochloride, methylene blue at the concentration of 0.5% and distilled water; and the pH is adjusted to 7.1. A fermentation culture method of the clear liquid fermentation medium for clostridium butyricum comprises the following steps of: glycerin tube refrigerated strain activation, heating and optimization, Erlenmeyer flask first-order seed culture, liquid fermentation and spray drying. The production of clostridium butyricum has advantages of simple operation, high amount of zymocyte, high gemma yield, simple post-treatment, less impurities in a bacterial powder sample and high amount of effective live bacteria, and saves cost at large.
Owner:HUAZHONG AGRI UNIV
Oxygen-resistant acid-resistant Bifidobacterium longum
InactiveCN102206599AImprove physiological and biochemical performanceImprove toleranceBacteriaMicroorganism based processesOxygenMicrobiological culture
The invention relates to oxygen-resistant acid-resistant Bifidobacterium longum, and is characterized in that: the name is CC-1, the category name is Bifidobacterium longum, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 4730, the preservation date is April 2, 2011, the preservation address is No.3, No.1 Court, Beichen West Road, Chaoyang District, Beijing, and the preservation institution is China General Microbiological Culture Collection Center. According to the invention, the physiological and biochemical properties of the bifidobacterium are effectively improved and the oxygen and acid tolerance of the bifidobacterium is increased; and oxygen-resistant acid-resistant Bifidobacterium longum is cultured in a PTYG (peptone-tryptone-yeast extract-glucose) liquid culture medium with a pH value of 4.0 in the presence of oxygen at the temperature of 37 DEG C, pour method viable count results show that the viable count of the cultured oxygen-resistant acid-resistant Bifidobacterium longum CC-1 can reach 4.7*10<7> CFU / ml, and the concentration has an important role in the research and application of bifidobacteria products.
Owner:TIANJIN UNIV OF SCI & TECH
Reinforced nutrient feed stuff for bee
InactiveCN101011114AComprehensive and balanced nutritionEasy to manufactureAnimal feeding stuffAccessory food factorsBiotechnologyYeast
The invention relates to a nourishment fodder for honey bees, which comprises soybean flour 100 parts, yeast powder 50-70 parts, tryptone 5-15 parts, bee honey 70-90 parts, water 30-50 parts, white sugar 10-30 parts, whole milk powder 3-5 parts, composite vitamin 1-3 parts and lypressin 0.5-1.5 parts.
Owner:GUANGDONG ENTOMOLOGICAL INST
Low-serum culture medium for efficiently culturing mycoplasma hyopneumoniae and preparation method thereof
ActiveCN103060220AIncrease the titer of live bacteriaReduce allergic reactionsAntibacterial agentsBacterial antigen ingredientsMycoplasma cultureOrganism
The invention relates to an efficient mycoplasma hyopneumoniae culture medium and a preparation method thereof, and belongs to the technical field of veterinary biology. The efficient mycoplasma hyopneumoniae culture medium comprises an A liquid and a B liquid mainly consisting of MEM, yeast leaching powder, tryptone, glucose, inorganic salt and the like. The prepared culture medium of the invention has the main advantages of low serum content which is only 10%-15%, while the serum content in common culture medium is 20% even more. The culture medium prepared by the low serum relieves the pig allergic to the stress reaction, meanwhile gives consideration to the biosafety of animals. Besides the valence of the semi-finished bacterial solution prepared by the method is up to 109CCU / ml, which is much higher than the culture medium prepared by the common technology.
Owner:兆丰华生物科技(南京)有限公司 +1
Fluorogenic selective and differential medium for isolation of Enterobacter sakazakii
Particular aspects provide novel compositions and methods useful for the growth, isolation and detection of microorganisms that have α-glucosidase activity (e.g., the bacterium E. sakazakii). Certain embodiments provide a novel growth and / or plating media, comprising a fluorogenic α-glucosidase substrate, which is both selective for and differential to E. sakazakii. In particular embodiments, the α-glucosidase substrate comprises 4-methylumbelliferyl-α-D-glucoside. Additional embodiments relate to a selection media. Further embodiments relate to a selective medium that is based on Tryptone Bile agar. Still further embodiments relate to OK media as defined herein. Other embodiments of the invention relate to methods for growing bacterial cultures on media that is selective for and differential to microorganisms that have α-glucosidase activity (e.g., the bacterium E. sakazakii).
Owner:WASHINGTON STATE UNIVERSITY
Heatproof lyoprotectant for live vaccine against pseudorabies and preparation method thereof, and lyophilized vaccine and preparation method thereof
ActiveCN107281481AFunction increaseImprove protectionViral antigen ingredientsAntiviralsMonosodium glutamateSucrose
The invention provides a heatproof lyoprotectant for a live vaccine against pseudorabies. The heatproof lyoprotectant comprises, by weight, 3 to 10 parts of gelatin, 1 to 5 parts of trehalose, 5 to 15 parts of sucrose, 0.1 to 2 parts bovine serum albumin, 1 to 8 parts of tryptone, 2 to 10 parts of enzyme-hydrolyzed casein, 1 to 5 parts of thiourea, 0.8 to 2 parts of L-monosodium glutamate, 0.1 to 3 parts of arginine, 0.5 to 5 parts of polyvinylpyrrolidone (PVP-K30) and 0.1 to 2 parts of mannitol. The invention also discloses a preparation method of the heatproof lyoprotectant and a lyophilized vaccine prepared from the heatproof lyoprotectant. When the heatproof lyoprotectant is used for protecting the vaccine and a specific lyophilization process is employed, lyophilization loss of viruses can be effectively reduced, the temperature tolerance of the viruses can be improved, and the vaccine can still maintain good physical properties and titer after long-term storage; i.e., the vaccine has stable characters and has the characteristics of heat resistance and long storage time.
Owner:SICHUAN HUAPAI BIO PHARMA
Salmonella characteristic chromogenic liquid nutrient medium, preparation method thereof and rapid detection method of salmonella
ActiveCN102433373AEasy to prepareEase of industrial productionMicrobiological testing/measurementMicroorganism based processesLithium chlorideDipotassium phosphate
The invention relates to the field of safety monitoring of food microorganisms, and discloses a salmonella characteristic chromogenic liquid nutrient medium, a preparation method thereof and a rapid detection method of salmonella. The salmonella characteristic chromogenic liquid nutrient medium comprises the following main components: tryptone, yeast powder, sodium chloride, lithium chloride, sodium deoxycholate, dipotassium phosphate, combined inhibitor, combined accelerator, characteristic enzymolysis substrate and cosolvent. The rapid detection method disclosed by the invention comprises two steps, i.e. pre-enrichment culture and chromogenic identification, and is characterized in that salmonella characteristic enzyme hydrolyzes corresponding substrates to result in that the culture medium is purple, thus rapidly judging the existence of salmonella; and the addition of the accelerator contributes to recovering damaged cells of salmonella and promoting growth of salmonella; and the added inhibitor can selectively inhibit the growth of other competitors so as to reduce the interference on detection by the competitors. The detection method disclosed by the invention has the advantages of short detection period, strong specificity and high accuracy, is simple to operate and is suitable for large-throughput detection of salmonella in food.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Method and reagent kit for enriching and sequencing peptide fragment containing histidine
InactiveCN101042377AGreat practicabilityIon-exchange process apparatusComponent separationProtein insertionAminoacidemia
This invention provides one new method and agent case for collecting and testing group aminoacidemia peptides, which comprises the following steps: protein restores alkyl base and tryptone protein enzyme cut, peptides section N end amidogen decoration and group aminoacidemia peptides color spectrum collection and mass spectrum testing sequence; identifying the protein with peptides sections in mixture objects.
Owner:INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
Haemophilus parasuis culture medium
ActiveCN103215208AMaintain integrityIncrease success rateBacteriaMicroorganism based processesSerum igeHaemophilus
The invention discloses a haemophilus parasuis culture medium. A preparation method of the haemophilus parasuis culture medium comprises the steps of preparing a basic culture solution, treating coenzyme A, and perfecting a culture medium, wherein the basic culture solution comprises peptone, tryptone, sodium chloride, dextrose, yeast extract, glycerin and distilled water, and the basic culture solution, the coenzyme A and fetal bovine serum are uniformly mixed so as to obtain the haemophilus parasuis culture medium. The haemophilus parasuis culture medium provided by the invention can keep haemophilus parasuis, thereby facilitating the transportation of suspicious haemophilus parasuis samples; and the death of haemophilus parasuis is not caused in the process of transportation, thereby increasing the separation probability of the haemophilus parasuis.
Owner:广西悦牧生物科技有限公司
Method of using wheat straws for producing bacterium cellulose
InactiveCN101525647AWide variety of sourcesLow priceMicroorganism based processesFermentationFiltrationCulture mediums
The invention relates to a method of using wheat straws for producing bacterium cellulose, which includes the following steps: wheat straws are milled into 40 to 80 meshes and immersed by dilute sulphuric acid or hydrochloric acid in a reaction container according to the solid / liquid ratio of 1:6 to 1:30 of the wheat straws to the dilute acid liquid, the wheat straws react with the dilute sulphuric acid or hydrochloric acid at the temperature of 100 DEG C to 121 DEG C, the residual slag of the wheat straws and the acid hydrolyzed liquid are separated by sucking filtration, and the hydrolyzed liquid is collected and refrigerated for spare; the hydrolyzed liquid is detoxified; the detoxified hydrolyzed liquid is used as the carbon source of culture medium, 0.1 percent to 1 percent of yeast concrete and 0.1 percent to 0.5 percent of tryptone are added to be prepared into culture medium, the acetobacter or the glucose oxidized bacilli is vaccinated with the vaccinating amount of 6 percent to 10 percent to be cultivated in an oscillating table of 36 to 30 DEG C and 160 to 250 r / m or statically cultivated in an incubator of 26 DEG C to 30 DEG C, and the bacterium cellulose is obtained. The carbon source of culture medium prepared by the method has good quality and low price, and can be used for industrial production.
Owner:DONGHUA UNIV
Method for preparing high-activity nattokinase by taking black beans as raw material
ActiveCN103205409AIncrease enzyme activityGreat tasteMicroorganism based processesEnzymesFreeze-dryingNutrient solution
The invention discloses a method for preparing high-activity nattokinase by taking black beans as raw material, belonging to the technical field of biology. The method comprises the following steps: soaking the black beans by using a special nutrient solution, cooking the black beans after soaking and further inoculating bacillus natto to perform fermentation, wherein the special nutrient solution comprises the following components: lactose, tryptone, Na2HPO4, NaH2PO4, MgSO4 and CaCl2. The method further comprises the steps of performing extraction separation and freeze-drying on a fermentation product. The nattokinase obtained by adopting the method has high enzyme activity, which can achieve 42500IU / g, and the nattokinase has the advantages of high enzyme activity, low ammonia smell and good taste in comparison with the nattokinase produced by taking yellow beans as the raw material and performing a traditional fermentation method; and the content of nutritional components in obtained fermented black bean residue is also obviously improved. The method has the advantages of short fermentation period, simple preparation process and high bioavailability, and is conductive to industrial production.
Owner:湖北真福医药有限公司
Selective culture medium used for quantitative detection of bacillus
ActiveCN102127518AHigh selectivityFast and accurate quantitative detectionBacteriaMicrobiological testing/measurementBiotechnologyMicroorganism
The invention discloses a selective culture medium used for the quantitative detection of bacillus. Each litre of the selective culture medium comprises the following components: 1-10g of glucose, 1-5g of yeast extract, 5-10g of tryptone, 0.2-2g of calcium chloride, 0.1-1g of magnesium sulfate, 15-20g of agar and 100-500IU of bacteriostatic agent. Through variable temperature cultivation, the selective culture medium adopts can be used to increase the selectivity of the conventional plate counting method to bacillus, eliminate the influences of other microorganisms or impurities in s sample to be detected and achieve the aim of detecting bacillus fast and quantitatively.
Owner:清远海贝生物技术有限公司
Solid-state freeze-dried product of quantitative strain and preparation method and using method thereof
The invention discloses a solid-state freeze-dried product of a quantitative strain and a preparation method and a using method thereof. 0.1% to 0.5% of glucose, 5% to 10% of sucrose, 0.5% to 1.5% of gelatin, 1% to 2% of tryptone, 0.5% to 1% of soy peptone, 0.5% to 1% of sodium chloride, 0.2% to 0.5% of yeast powder and 0.2% to 0.5% of activated carbon are prepared into solution serving as a protective agent, and after the solution and diluted bacteria suspension are uniformly mixed according to a certain proportion, the mixture is prepared into the dried product by freezing-drying; the product is excellent in solubility and can be completely dissolved in a dissolving agent in 1 to 2 seconds. The cylindrical freeze-dried product has an amount of bacteria of 100cfu to 1,000cfu or can customize bacterium content in a specific range. One single freeze-dried product is taken and dissolved into N ml of dissolving agent, and the bacterium content per N tenths ml is smaller than 100cfu, so that the solid-state freeze-dried product is accurate and convenient. Use of the protective agent can greatly reduce a damage degree of the strain in the freeze-drying process; the proper protective agent provides an excellent shape and freeze-drying environment for the product so as to guarantee activity of the strain to be protected to the greatest degree. The dissolving agent provides an excellent pH value, an excellent osmotic pressure and proper nutrient substances to the strain so as to guarantee the strain to obtain the optimal recovery conditions after the strain is dissolved.
Owner:浙江泰林生命科学有限公司
Culture medium used for separating and screening lactic acid bacteria, preparation method and application thereof
The invention provides a culture medium used for separating and screening lactic acid bacteria. The formula of the culture medium comprises the following components: relative to the dosage of 1000ml of a solvent, 5.0-15.0g of tryptone, 5.0-15.0g of beef extract, 2.0-10.0g of yeast extract, 1.0-3.0g of diammonium citrate, 15.0-25.0g of glucose, 0.2-2.0 mL of Tween-80, 2.0-8.0g of sodium acetate, 15.0-20.0g of agar, 2.0-30.0g of calcium carbonate, a compound acid-base indicator containing 0.01-0.5g of methyl red and 0.01-0.7g of bromothymol blue, 0.5-5.0g of ascorbic acid, 0.1-0.5g of L-cysteine HCl, and a selective antibiotic composed of 250000-500000 IU of polymyxin B and 0.01-0.05g of nalidixic acid, wherein the pH value of the culture medium is 6.8; and the solvent is composed of a primary solvent and a secondary solvent, the primary solvent is distilled water or aged seawater, and the secondary solvent is saline solution. The culture medium has the characteristics of being high in accuracy, obvious in authentication phenomenon and the like during separation and screening for lactic acid bacteria.
Owner:江门市澳保生物科技有限公司
Method for enriching and sequencing protein terminal peptide fragment and reagent kit
InactiveCN101042374AApplicable identificationApplicable terminal analysisIon-exchange process apparatusComponent separationProtein insertionTest sequence
This invention provides one collection and testing sequence for protein end enriching and testing method and test case, which comprises the following steps: protein end amidogen base decorating, alkali restoring base and tryptone enzyme slices, end peptide enrich end and mass spectrum test sequence. The invention through enzyme slice peptide mixture and end peptide section enriching and testing to get the protein end sequence information for protein testing and end analysis for protein group study. This invention also describes this method agent case.
Owner:INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
Method for preparing bacterial cellulose from corn stalks
InactiveCN102051395AImprove hydrolysis efficiencyEasy to produceFermentationEconomic benefitsCellulase
The invention relates to a method for preparing bacterial cellulose from corn stalks. The method comprises the following steps: pretreating the corn stalks in an ionic liquid at 90-120 DEG C for 15 minutes-21 hours, then adding deionized water, stirring for regeneration, and adding cellulase to carry out enzymolysis, thereby obtaining an enzymolysis liquid; and detoxifying the enzymolysis liquid to obtain a culture medium for culturing the bacterial cellulose, adding yeast extract and tryptone to obtain a culture medium, adding a seed liquid of bacterial cellulose producing bacteria, and standing to culture at 25-30 DEG C for 8-28 days, thereby obtaining the bacterial cellulose. According to the invention, the farm crop byproduct corn stalks, which are often discarded or incinerated, are utilized as a raw material, thus a cheap carbon source is developed, which reduces the environmental pollution and can be used for producing products with high added value, thereby creating high economic benefit for related industries.
Owner:DONGHUA UNIV
Bacterium and fungus strain storage culture medium
The invention relates to a bacterium and fungus strain storage culture medium comprising the following components: tryptone, sodium chloride, agar, yeas extract and glycerol. The tryptone is used for providing a microorganism nitrogen source; the sodium chloride is used for maintaining a uniform osmotic pressure; the agar is used as a supporting component in the culture medium; the yeas extract is rich in proteins, amino acids, peptides, nucleotides and vitamins B and can be used for replenishing various vitamins, amino acids and growth factors for the growth of microorganisms; and the glycerol is used as a carbon source and can be used for playing roles of preventing freeze and preserving moisture. The bacterium and fungus strain storage culture medium is simple and convenient in operation of strain storage, long in storage time, high in stability and capable of increasing the survival rate and the reproductive rate of the strains.
Owner:苏桂华
Butyric acid bacterium fermentation culture medium and preparation method thereof and butyric acid bacterium culture fermentation method
ActiveCN103805549ALow priceWide variety of sourcesBacteriaMicroorganism based processesCulture mediumsGlucic acid
The invention provides a butyric acid bacterium fermentation culture medium and a preparation method thereof and a butyric acid bacterium culture fermentation method. The butyric acid bacterium culture medium includes a nitrogen source, a carbon source and sodium chloride, wherein the nitrogen source is one or any combination of fish meal, bean pulp or yeast extract, and the nitrogen source is glucose and / or corn flour. The number of butyric acid bacteria can stably achieve more than 109CFU / mL after the fermentation culture medium is adopted for fermentation for 14-16h; according to the fermentation culture medium, the nitrogen source and the carbon source which are widely available are used for replacing tryptone, glucose and the like; the fermentation cost is low, the fermentation time is shortened, the fermentation efficiency is improved, and the production cost is greatly lowered.
Owner:TIANJIN SHENGJI GRP CO LTD
Bifidobacterium bifidum and application thereof
ActiveCN108707565ACan regulate immune responseEnhanced inhibitory effectBacteriaLactobacillusEscherichia coliMortality rate
Owner:BEIJING BOJINYUAN BIOTECH
Clostridium butyricum as well as colstridium butyricum feed additive and preparation method thereof
InactiveCN102550818ALow costImprove factory efficiencyBacteriaAnimal feeding stuffBiotechnologyDipotassium hydrogen phosphate
The invention discloses a method for preparing a clostridium butyricum feed additive and a clostridium butyricum feed additive prepared by the method. The method for preparing the clostridium butyricum feed additive is characterized by adopting clostridium butyricum (the clostridium butyricum is preserved in the China Center of Industrial Culture Collection and the serial number is CICC 20036). The method comprises the following steps: such materials as tryptone, beef extract, yeast cream, glucose, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, magnesium sulphate, calcium chloride, ferrous sulfate, cysteine hydrochloride and the like are prepared into a dedicated liquid culture medium for seeds; strains are grafted into the culture medium for activating seeds; nitrogen source, carbon source and the like are prepared into a solid anerobic fermentation culture medium; and the seed liquid is grafted into the solid anerobic fermentation culture medium for solid state fermentation and then is dried and sieved. By utilizing the cheap nitrogen and carbon sources to form the medium, the invention provides a novel method for large-scale and industrial production of clostridium butyricum; and the method reduces the cost effectively and increases the benefits of plants.
Owner:SHANGHAI CHUANGBO ECOLOGICAL ENG
Nematode-eating fungus with nematode-killing function, preparing method and use thereof
The present invention is one kind of nematode eating fungi capable of killing nematode and its preparation process and application, and belongs to the field of microbial pesticide technology. The nematode eating fungus is obtained through liquid fermentation of the strain, extracting the effective nematode killing component from the metabolic product and subsequent preparation step. The nematode eating fungus strain is Dactylella shizishanna YMF1.00022, and the liquid fermentation culture medium consists of gelatin, tryptone, yeast leaching powder, ammonium sulfate, ferrous sulfate, magnesium sulfate, disodium hydrogen phosphate, sodium dihydrogen phosphate and water in certain proportion. The extracting process of the effective nematode killing component includes taking supernatant from the fermented matter, adding ammonium sulfate, centrifuging, dissolving in buffering phosphoric acid solution, dialysis and other steps.
Owner:YUNNAN UNIV
Manufacturing method of purple puffball liquid strain
The invention discloses a manufacturing method of a purple puffball liquid strain, which is formed in a mode that purple puffball sporocarps, potato, trypsin peptone, carbamide, glucose, beef extract, corn kernels, vitamin B1, dipotassium phosphate, magnesium sulfate, calcium chloride and the like are used for manufacturing a purple puffball liquid medium which is subjected to medium degerming, inoculating and strain culturing. The manufacturing method has prominent characteristics compared with the prior art. The manufacturing method of the purple puffball liquid strain is simple, the production cost is reduced by more than 20%, and the strain pollution is reduced by more than 30%; the myceilum biomass (125.55mk / kg) is higher than that of the traditional method by 20mg / kg, and the infection intensity (62%) of the strain to trees is higher than that of the traditional method by more than 15%. The method can realize the scale and industrialization production of the purple puffball liquid strain and develops a new wealth acquiring route for mountain area peasants in Guizhou Province.
Owner:GUIZHOU INST OF BIOLOGY
Liquid medium used for culturing haemophilus parasuis
InactiveCN106282075APromote growthImprove securityBacteriaMicroorganism based processesLiquid mediumAdditive ingredient
The invention provides a liquid medium used for culturing haemophilus parasuis. The liquid medium comprises the following components: tryptone, soya peptone, sodium chloride, dipotassium phosphate, glucose, yeast powder, bovine serum and a coenzyme I, and the liquid medium has a pH value of 7.2-7.4. The liquid medium has the beneficial effects that the liquid medium is beneficial to growth of haemophilus parasuis by supplementing the nutritional ingredients required by various microorganisms and effectively controlling the pH value in the medium via sodium hydroxide; the viable counts are not lower than 10<9>CFU / ml; and inactivated vaccines are prepared from haemophilus parasuis liquid and have good safety and immune efficacy.
Owner:YEBIO BIOENG OF QINGDAO
Mycoplasma anatis culture medium and separation and purification method for mycoplasma anatis
ActiveCN105462884AHigh in nutrientsExtended storage timeBacteriaMicroorganism based processesBiotechnologyMycoplasma culture
The invention provides a mycoplasma anatis culture medium. The mycoplasma anatis culture medium is composed of a liquid culture medium and a solid culture medium. The liquid culture medium is prepared from deionized water, a 10% thallium acetate solution, PPLO broth, glucose, peptone, tryptone, 1% phenol red, a CMRL-1066 culture medium containing L-glutamine, inactivated horse serum, yeast leachate, penicillin, L-cysteine hydrochloride and nicotinamide adenine dinucleotide. Besides the situation that agar powder is added and phenol red is removed, other components of the solid culture medium are the same as those of the liquid culture medium. The culture medium is used for separating and purifying mycoplasma anatis, wherein a bacterial colony is separated from the solid culture medium, the separated bacterial colony is placed in the liquid culture medium to grow, then filtering, dilution and plate coating of liquid culture substances and purification of picked single colony multiplication are conducted, and the steps are repeated till purified mycoplasma anatis is obtained. The culture medium can promote growth of mycoplasma anatis, and the success rate of separation and purification of mycoplasma anatis is high.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Staphylococcus aureus chromogenic medium and test piece thereof
ActiveCN103290094AHigh selectivityGrowth inhibitionBioreactor/fermenter combinationsBiological substance pretreatmentsStaphylococcus cohniiMonopotassium phosphate
The invention discloses staphylococcus aureus chromogenic medium and a test slip thereof. The chromogenic medium contains tryptone, yeast extract powder, beef extract powder, glucose, sodium pyruvate, glycine, sodium chloride, monopotassium phosphate, disodium hydrogen phosphate, a bacteriostatic agent mixture and two specific enzyme chromogenic substrates. The chromogenic medium and the test slip made by using the chromogenic medium can be used for quickly detecting staphylococcus aureus in a large-flux sample, are obvious in positive result color development, accurate in tested result, strong in specificity, high in flexibility, short in test period, simple and convenient to operate, low in test environment requirement and test personnel, and capable of carrying out quantitative analysis while carrying out qualitative test on the staphylococcus aureus, and therefore, the chromogenic media and the test slip are very suitable for being used by grass-roots supervisory authorities and enterprises, and are extensive in application prospect.
Owner:GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD
High-nutrition white fungus beverage having typical flavor of white funguses, and making method of high-nutrition white fungus beverage
ActiveCN108041383ATypical tremella flavorHigh in functional nutrientsFood ingredient functionsPolysaccharide/gum food ingredientsAdditive ingredientTremella
The invention provides a making method of a high-nutrition white fungus beverage having typical flavor of white funguses. According to the method, special mixed bacteria are used for fermentation. Themethod comprises the following steps of (1) preparing fermentation substrates, wherein the fermentation substrates consist of the following components in percentage by weight: 0.2% of food grade glucose, 0.1-0.2% of food grade casein tryptone, 0.05-1% of a yeast extract and the balance of a white fungus polysaccharide extraction solution; (2) sterilizing the fermentation substrates, inoculating the sterilized fermentation substrates with mixed bacteria, and performing vibrating fermentation culture at 32-35 DEG C for 3-7 days so as to obtain a fermentation solution; and (3) adding an appropriate quantity of food auxiliary materials to the fermentation solution obtained in the step (2) so as to obtain the high-nutrition white fungus beverage. The white fungus polysaccharide fermentation solution has typical white fungus flavor, contains rich functional nutrient components and contains various probiotics, so that functional requirements of people for a plant-derived beverage are met, and the flavor requirements of people for a plant-derived beverage are also met.
Owner:龚雪梅 +1
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