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Haemophilus parasuis culture medium

A technology of Haemophilus suis and culture medium, which is applied in the field of agricultural microorganisms, can solve the problems of culture medium not suitable for Haemophilus parasuis, unsuitable culture medium, etc., so as to improve the separation rate of bacteria, improve the probability of bacteria production, and maintain Integrity effect

Active Publication Date: 2013-07-24
广西悦牧生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, TSA and TSB are not suitable as a medium for transporting suspicious samples, nor as a medium for preserving Haemophilus parasuis

Method used

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  • Haemophilus parasuis culture medium

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Experimental program
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Effect test

Embodiment 1

[0026] Take 15g / L peptone, 5g / L tryptone, 5g / L sodium chloride, 2g / L glucose, 5g / L yeast extract and 15‰ glycerin, mix the remaining distilled water, heat to boiling and keep stirring until the mixture is completely dissolved , adjust the pH value of the solution to 7.0~7.2 with sodium hydroxide, divide the solution with adjusted pH value, sterilize it at 121°C for 15~20min, and obtain the basic culture medium after cooling; take 0.15g / L coenzyme A. Dissolve, dilute, filter and sterilize with 10‰ sterile water; add 1ml of coenzyme A dilution and 5ml of sterile calf serum to each 100ml of basic culture solution through aseptic procedures, mix well, and divide the medium into test tubes. Take 10-15ml of each test tube and store at 4-5°C to obtain Haemophilus parasuis culture medium.

Embodiment 2

[0028] Take 14g / L peptone, 6g / L tryptone, 5g / L sodium chloride, 2g / L glucose, 5g / L yeast extract and 15‰ glycerin, mix the rest with distilled water, heat to boiling and keep stirring until the mixture is completely dissolved , adjust the pH value of the solution to 7.0~7.2 with sodium hydroxide, divide the solution with adjusted pH value, sterilize it at 121°C for 15~20min, and obtain the basic culture medium after cooling; take 0.14g / L coenzyme A. Dissolve, dilute, filter and sterilize with 8‰ sterile water; add 1ml of coenzyme A dilution and 5ml of sterile calf serum to each 100ml of basic culture solution through aseptic procedures, mix well, and divide the medium into test tubes. Take 10-15ml of each test tube and store at 4-5°C to obtain Haemophilus parasuis culture medium.

Embodiment 3

[0030] Take 16g / L peptone, 4g / L tryptone, 4g / L sodium chloride, 3g / L glucose, 4g / L yeast extract and 13‰ glycerin, mix the rest with distilled water, heat to boiling and keep stirring until the mixture is completely dissolved , adjust the pH value of the solution to 7.0~7.2 with sodium hydroxide, divide the solution with adjusted pH value, sterilize it at 121°C for 15~20min, and obtain the basic culture medium after cooling; take 0.15g / L coenzyme A, dissolve and dilute with 10‰ sterile water and filter out the bacteria; add 1ml of coenzyme A dilution and 5ml of sterile calf serum to each 100ml of basic culture solution in a sterile procedure, mix well, and divide the medium into test tubes. Take 10-15ml of each test tube and store at 4-5°C to obtain Haemophilus parasuis culture medium.

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Abstract

The invention discloses a haemophilus parasuis culture medium. A preparation method of the haemophilus parasuis culture medium comprises the steps of preparing a basic culture solution, treating coenzyme A, and perfecting a culture medium, wherein the basic culture solution comprises peptone, tryptone, sodium chloride, dextrose, yeast extract, glycerin and distilled water, and the basic culture solution, the coenzyme A and fetal bovine serum are uniformly mixed so as to obtain the haemophilus parasuis culture medium. The haemophilus parasuis culture medium provided by the invention can keep haemophilus parasuis, thereby facilitating the transportation of suspicious haemophilus parasuis samples; and the death of haemophilus parasuis is not caused in the process of transportation, thereby increasing the separation probability of the haemophilus parasuis.

Description

[0001] technical field [0002] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a culture medium for Haemophilus parasuis. Background technique [0003] Haemophilus parasuis can cause polyserositis and arthritis in pigs. The bacterium is very delicate and has relatively strict requirements on the medium. Its growth depends on nicotinamide adenine dinucleotide (NAD or V factor) and does not require X factor (heme and other porphyrin substances). Its growth rate is extremely slow. In vitro It is easy to die in culture and storage. It can grow pinpoint-sized colonies only after culturing on the blood agar plate for more than 72 hours. HPS is easily ignored or covered by other bacteria. [0004] Haemophilus parasuis is sensitive to freezing and heat. Samples of isolated bacteria, such as joint fluid and pleural effusion, should be inoculated with culture medium immediately, otherwise the isolation and culture often canno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/21
Inventor 陈泽祥李军杨威彭昊许力干谢宇舟禤雄标胡帅马春霞谢永平潘艳
Owner 广西悦牧生物科技有限公司
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