35 results about "Coenzyme A" patented technology

The invention relates to a composition with the efficacies of whitening, moisturizing, repairing and caring the skin, as well as a preparation method and applications of the composition. The skin care composition is prepared from the following raw materials: water, sodium hyaluronate, sodium dihydrogen phosphate dihydrate, potassium chloride, sodium hydroxide, calcium chloride, glutamine, benzalkonium chloride, magnesium sulfate, aminobutyric acid, sodium ascorbate, alanine, arginine, lysine hydrochloride, valine, histidine, leucine, taurine, coenzyme A, alcohol, polysorbate 80, thiamine, disodium diphosphate, recombinant human epidermal cell growth factors and the like. The skin care composition disclosed by the invention can permeate the muscle bottom for replenishing water for the skin from the deep layer, so that the skin is full of elasticity, the color of the skin is brightened, the fine wrinkles are reduced, the skin is clean and watery, the balance of water and oil is regulated, the pores are shrunk, meanwhile, the brightness of the face can be increased, the injured epidermal layer is effectively repaired, the activity of tyrosinase is inhibited, the generation of skin melanin is reduced, so that the skin is healthy, natural and white.

The present invention provides a method for stabilizing reduced coenzyme Q10, which is useful as a food, nutritional product, nutritional supplement, animal drug, drink, feed, cosmetic, pharmaceutical product, therapeutic drug, prophylactic drug and the like. The present invention also provides a method of producing a reduced coenzyme Q10-containing composition which includes the co-presence of reduced coenzyme Q10 and reduced coenzyme Q9 and / or reduced coenzyme Q11.

The invention relates to the preparation of a wild cactus polyoses extract and the highly effective function thereof for reducing serum cholesterase. The extract is a polyoses extracted from the natural wild cactus polyoses, and can reduce the total cholesterol content in the hypercholesterolemia and obviously improve the lipid metabolic disturbance by improving the activity of in vivo lipid metabolic key enzyme lecithin cholesterin acylase and reducing the activity of synthetical rate-limiting enzyme 3-hydroxy-3-methyl pentane diacid coenzyme A reductase of liver cholesterin; the invention has no obviouse adverse reaction and can be used for preparing medicines used for lowering the cholesterin.

The present invention deals with a method for the preparation of 2,4- dihydroxybutyric acid (2,4-DHB) comprising the successive steps of converting malate, succinyl-CoA and / or glyoxylate into malyl-CoA, converting malyl-CoA previously obtained into malate-4-semialdehyde, and converting malate-4- semialdehyde into 2,4-DHB using a DHB dehydrogenase.

The invention provides a single-excitation double-emission carbon-based biomass dot as well as a preparation method and application thereof. The preparation method comprise S1, a proper number of clean Chinese cabbage leaves are weighted, and are ground in a utensil; S2, after absolute ethyl alcohol and an acetone solution according to the volume ratio of 1:0.9 to 1:1.1 are added, and then the mixture is uniformly mixed for being soaked; S3, after being soaked for 28-32 minutes, filtering is conducted to obtain a chlorophyll extracting solution; S4, 25 mL of chlorophyll extracting solution proportional to 0.1g-0.11g of polyoxyethylene diamine are added in a polytetrafluoroethylene reaction kettle, and mixing and heating are conducted for 9-11h under the temperature controlled to be 195-205DEG C; S5, after cooling to the room temperature is conducted, suction filtration is conducted, and a filtrate is adjusted to that with a pH value roundly equal to 7; and S6, the filtrate is transferred into a dialysis bag for lucifugal dialysis for 11-13h to obtain the single-excitation double-emission carbon-based biomass dot. According to the dot and the preparation method and application thereof, the carbon-based biomass dot can be directly used as a probe for ratiometric fluorescence imaging of active substance coenzyme A in cells, the background fluorescence interference of biological tissue can be eliminated, and the accuracy and the sensitivity of the fluorescence imaging of the coenzyme A can be improved.

The invention discloses a technique for biotransforming CO2 into isopropyl alcohol by utilizing blue algae, and provides the following six methods for preparing recombined blue algae: (1) leading a CoA transferase gene, an acetoacetate decarboxylase gene and an alcohol dehydrogenase gene into the blue algae; (2) leading the CoA transferase gene and the acetoacetate decarboxylase gene into the blue algae; (3) leading a coded gene of the alcohol dehydrogenase into the recombined blue algae in (2); (4) leading an acetyl-CoA acetyltransferase gene, an acetoacetyl-CoA transferase gene, the acetoacetate decarboxylase gene and the alcohol dehydrogenase gene into the blue algae; (5) leading the acetyl CoA acetyltransferase gene, the acetoacetyl-CoA transferase gene and the acetoacetate decarboxylase gene into the blue algae; and (6) leading the alcohol dehydrogenase gene into the recombined algae generating acetone in (5). The technique provided by the invention is one of ideal paths for realizing the continuous and healthy development of renewable and clean energy by utilizing the recombined algae to produce acetone and the isopropyl alcohol.

The invention discloses a nasal cavity and paranasal sinus-applied medicine for treating obesity and a preparation method thereof, a product adopts 11 necessary ingredients of coenzyme A, carnitine, methionine, choline, inositol, vitamin B1, vitamin B2, vitamin B6, niacin, folic acid and vitamin B12, and a multi-purpose liquid carrier for paranasal sinus medicine application is matched for preparation. All necessary factors and adding quantities thereof are selected according to the full process of fat metabolism; the carrier is liquid with protection role for a nasal cavity or paranasal sinuses; and the necessary factors can be directly sent to the paranasal sinuses with abundant blood and be directly absorbed for entering into the blood circulation through the nasal cavity and paranasal sinus medicine application, thereby realizing high absorption efficiency and no stimulus to the gastrointestinal tract, accelerating the fat metabolism, gradually reducing excess fat to the degree of ideal body weight and further being a treatment method with simple and easy operation, safety and effectivity.

This invention provides compounds of Formulae (IA), (IB), (IC), (ID), (IE), (IF), (IG), (IH), (IJ), (IK), (IL), (II), (III), (IIIA), and (IIIB); pharmaceutically acceptable salts and solvates thereof; and compositions thereof. This invention further provides methods for treating a disease, including but not limited to, liver disease or an abnormal liver condition; cancer (such as hepatocellular carcinoma or cholangiocarcinoma); a malignant or benign tumor of the lung, liver, gall bladder, bile duct or digestive tract; an intra- or extra-hepatic bile duct disease; a disorder of lipoprotein; a lipid-and-metabolic disorder; cirrhosis; fibrosis; a disorder of glucose metabolism; a cardiovascular or related vascular disorder; a disease resulting from steatosis, fibrosis, or cirrhosis; a disease associated with increased inflammation (such as hepatic inflammation or pulmonary inflammation); hepatocyte ballooning; a peroxisome proliferator activated receptor-associated disorder; an ATP citrate lyase disorder; an acetyl-coenzyme A carboxylase disorder; obesity; pancreatitis; or renal disease.

Disclosed are a method and a composition for stabilizing a reduced coenzyme Q10 which is useful as a food, a food with a nutrient function, a food for specified health uses, a nutritional supplement, a nutritional product, a veterinary drug, a beverage, a feeding stuff, a cosmetic, a pharmaceutical product, a therapeutic agent, a prophylactic agent and the like. The method is characterized by allowing a reduced coenzyme Q10 to coexist with a reduced coenzyme Q9 and / or a reduced coenzyme Q11. The composition comprises a reduced coenzyme Q10 and a reduced coenzyme Q9 and / or a reduced coenzyme Q11.

The present invention is about the tecnnique of production of coenzyme Q10 with enzyme engineering method, with Coenzyme Q0 and 10 polyisoprene or coenzyme Q1 and solanesol as raw materials, for coenzyme Q0, coenzyme Q1, 10 polyisoprene phosphorylated and Solanesol bromided. With the action of polymerase, Coenzyme Q10 was obtained in a successive process of extraction, concentration, crystallization, drying at the temperature or 40 deg.C to 60 deg.C. The described Polymerase was obtained as follows:choosing and cultivating the red Pseudomonas photosynthetic bacteria containing coenzyme Q10; breaking bacterial cells and obtaining sediment with 3% saline;obtaining Polymerase through separating sediments. The present invention is specified and improves the reaction efficiency and product quality. Coenzyme Q10,produced by the enzyme engineering method, does not exist Isoprene with cis-trans in its Side Chain.The product biological activity is identical to that made with fermentation, and production costs much lower.

The invention relates to a delta-5-desaturase from a Corbrin-production bacterium cordyceps sinensis hirsutella sinensis. The delta-5-desaturase participates in eicosapentaenoic coenzyme A anabolism started from eicosatetraenoic coenzyme A. The invention also relates to a gene coding the enzyme, and an application thereof. The amino acid sequence of the delta-5-desaturase has more than 90% homology with a sequence represented by SEQ ID No.1 or SEQ ID No.3. From the principle, the invention carries out detailed researches upon the metabolic pathway of eicosatetraenoic coenzyme A to synthesizing the eicosapentaenoic coenzyme A. A cloned DNA comprising the nucleotide sequence provided by the invention can be transferred to engineering bacteria through methods of transduction, transformation, and combined transferring. Through the regulation upon eicosapentaenoic coenzyme A biosynthetic gene expression, high expression of a host delta-5-desaturase is provided, an effective approach is provided for increasing eicosapentaenoic acid yield. The application has an important application prospect.

The present invention relates to the normal pressure catalytic hydrogenation process of preparing coenzyme Q0 hydroquinone and the refining process. The preparation and refining process includes mainly the normal pressure catalytic hydrogenation of coenzyme Q0 under the action of 5 % Pd / C catalyst, and washing with non-polar solvent for refining to eliminate un-reacted matter and impurity. The process has no high pressure operation, easy separation of product and no water related operation to result in loss of the product, so that the present invention has high product yield and purity and low cost.

The invention discloses a medicine for treating myocarditis. The medicine is prepared from the following materials in parts by weight: 2-6 parts of ginseng, 4-8 parts of radix scrophulariae, 6-10 parts of salvia miltiorrhiza, 8-12 parts of astragalus mongholicus, 2-3 parts of honeysuckles, 2-4 parts of pseudo-ginseng, 4-8 parts of leech, 2-4 parts of Szechuan Lovage Rhizome, 10-15 parts of radix isatidis, 8-12 parts of folium isatidis, 3-6 parts of polygala, 8-12 parts of wild jujube seeds, 8-12 parts of angelica, 5-13 parts of schisandra chinensis, 3-12 parts of liquorice and 1-2 parts of vermilion cinnabar. The medicine further comprises cinnamyl aldehyde, creatinine, vitamin C, vitamin E, adenosine-triphosphate, coenzyme A, potassium chloride, insulin, glucose, coenzyme Q10, and the like. The medicine has the effects of preventing virus, diminishing inflammation, clearing away heat, tonifying heart and replenishing the blood, soothing the nerves and tonifying qi. By combining traditional Chinese and western medicine, the medicine has remarkable curative effect on treating delirium, deficiency of heart-blood, myocarditis, heart strain, angina, worry beyond measure and amnesia.

The present invention provides a method for stabilizing reduced coenzyme Q10, which is useful as a food, nutritional product, nutritional supplement, animal drug, drink, feed, cosmetic, pharmaceutical product, therapeutic drug, prophylactic drug and the like. The present invention also provides a method of producing a reduced coenzyme Q10-containing composition which includes the co-presence of reduced coenzyme Q10 and reduced coenzyme Q9 and / or reduced coenzyme Q11.

The invention provides an application of a eutectic compound composed of a proton pump inhibitor and a hydroxymethylglutaryl coenzyme A reductase inhibitor in preparation of an antitumor drug. The compound is characterized in that: the proton pump inhibitor is selected from one of omeprazole, esmeprazole, pantoprazole, rabeprazole and lansoprazole; and the hydroxymethylglutaryl coenzyme A reductase inhibitor is selected from one of atorvastatin, rosuvastatin, simvastatin, fluvastatin, pravastatin, lovastatin and pitavastatin or pharmaceutically acceptable salt of the atorvastatin, rosuvastatin, simvastatin, fluvastatin, pravastatin, lovastatin and pitavastatin. The antitumor drug can be used for treating one of liver cancer, lung cancer, stomach cancer, ovarian cancer, colon cancer, cervical cancer, oral squamous cell carcinoma and leukemia.

The invention relates to the field of cosmetics and manufacturing of the cosmetics, in particular to a moisturizing, antioxidant and freckle-removing cream and a preparation method of the moisturizing, antioxidant and freckle-removing cream. The invention discloses an anti-aging cosmetic which is prepared from 1, 2-butanediol, squalane, alpha-arbutin, glycerol, polyglycerol-10 oleate, isopropyl palmitate, cetearyl glucoside, glyceryl stearate, cetostearyl alcohol, a liquorice root extract, a polygonum cuspidatum extract, vitamin E, carbomer, phenethyl resorcinol, a glycyrrhiza glabra extract, allantoin, polyglutamic acid, ethylhexylglycerin, coenzyme A and phenoxyethanol. Arginine and a mint extract. The lipidosome and the skin care product composition have good whitening and freckle-removing effects, are beneficial to increase of skin elasticity and water content of cuticle, and are simple in preparation process and suitable for industrial large-scale production.

The invention relates to a medicine for treating myocarditis. The medicine is prepared from a traditional Chinese medicine part and a western medicine part; the traditional Chinese medicine part comprises 20g of poria cocos, 15g of cassia twig, 15-20g of astragalus membranaceus, 10-15g of codonopsis pilosula, 10-15 g of caulis spatholobi, 9-12 g of loranthus parasiticus, 6-9 g of peach kernel, 6-9g of safflower, 10-15 g of angelica sinensis, 10-15 g of atractylodes macrocephala, 15-20 g of rhizoma alismatis, 10-15 g of fingered citron, 10-15 g of allium macrostemon, 10-12 g of trichosanthes kirilowii, 8-12g of fried jujube kernel, 10-12g of semen boitae, 6-9g of radix sophorae flavescentis, 9-12g of poria with hostwood and 9g of liquorice root; the western medicine part comprises vitaminB12, coenzyme A, inosine and cytochrome C. when the medicine is used for a patient with myocarditis caused by viral infection, antibiotics and vitamin C should be added. The above six western medicines have the effects of improving myocardial metabolism, sterilizing and inhibiting bacteria. The traditional Chinese and western medicine method is adopted for treating myocarditis, the treatment effect directly reaches the focus, and the recurrence rate is low.

The invention relates to an LCD (Lactoyl Coenzyme A Dehydratase)-PCT (Propionic Acid Coenzyme A Transferase) gene serial recombinant expression vector and an application thereof. The vector is established by connecting a PCT gene for encoding a PCT and an LCD gene for encoding an LCD in series, wherein the PCT gene and the LCD gene are respectively cloned from a clostridium propionicum genome; the vector is converted to an escherichia coli competent cell, and the expression of the PCT and the LCD is realized in the escherichia coli competent cell, so that an LCD-PCT recombinant genetically engineered bacterium is obtained; and an LCD-PCT catalytic active liquid obtained after the engineered bacterium is cultured and broken contains the PCT and the LCD which can be used for effectively catalyzing D-lactate to be converted into acrylic acid with the yield of about 41%, so that the efficient biological conversion of the acrylic acid can be realized, the production cost can be reduced, the environment pollution can be relieved, and the vector has very high production and application values.

The invention discloses a traditional Chinese medicine prescription for treating peripheral neuromuscular diseases of neurological department. The traditional Chinese medicine prescription consists of the following raw materials: 8-10 parts of radix ginseng rubra, 10-14 parts of radix astragali, 15-20 parts of radix paeoniae alba, 5-9 parts of fructus evodiae, 20-24 parts of fructus chaenomelis lagenariae, 8-12 parts of betel nuts, 8-10 parts of herba periliae, 8-12 parts of radix platycodonis, 12-16 parts of vermilion, 10-20 parts of dried orange peel, 10-14 parts of caulis akebiae, 5-9 parts of liquorice root, 4-10 parts of herba lycopodii, 8-12 parts of caulis spatholobi, 4-10 parts of radix dipsaci, 6-10 parts of fructus psoraleae, 8-12 parts of poria, 8-12 parts of frankincense, 4-10 parts of myrrh, 10-14 parts of radix aucklandiae, 12-16 parts of rhizoma corydalis, 0.2-0.4 part of vitamin B1, 0.2-0.6 part of vitamin B6, 0.1-0.3 part of triphosadenine, 0.2-0.4 part of coenzyme A and 0.2-0.6 part of nicotinic acid. The traditional Chinese medicine prescription provided by the invention, in comparison with the prior art, has the characteristics of being convenient in administration, low in toxic and side effects, good in therapeutic effect, short in curing time and less in recurrence.

This invention provides compounds of Formulae (IA), (IB), (IC), (ID), (IE), (IF), (IG), (IH), (IJ), (IK), (IL), (II), (III), (IIIA), and (IIIB); pharmaceutically acceptable salts and solvates thereof; and compositions thereof. This invention further provides methods for treating a disease, including but not limited to, liver disease or an abnormal liver condition; cancer (such as hepatocellular carcinoma or cholangiocarcinoma); a malignant or benign tumor of the lung, liver, gall bladder, bile duct or digestive tract; an intra- or extra-hepatic bile duct disease; a disorder of lipoprotein; a lipid-and-metabolic disorder; cirrhosis; fibrosis; a disorder of glucose metabolism; a cardiovascular or related vascular disorder; a disease resulting from steatosis, fibrosis, or cirrhosis; a disease associated with increased inflammation (such as hepatic inflammation or pulmonary inflammation); hepatocyte ballooning; a peroxisome proliferator activated receptor-associated disorder; an ATP citrate lyase disorder; an acetyl-coenzyme A carboxylase disorder; obesity; pancreatitis; or renal disease.

The invention relates to a homocysteine diagnosing / measuring reagent (kit) using the technologies of an enzyme colorimetric method and an enzyme linkage method. At the same time, the invention also relates to a homocysteine concentration measuring method, the reagent composition and reagent ingredients, which belong to the technical field of medical detection and measurement. The reagent (kit) mainly comprises the following ingredients: a buffer solution, cozymase, glutathione disulfide, cozymase A-glutathione, acetaldehyde, glutathione homocysteine transhydrogenase, glutathione-cozymase A-glutathione transhydrogenase, acetaldehyde dehydrogenase and stabilizing agents. The method has the following steps: mixing samples and the reagent by the certain volume percent for making the samples and the reagent take a series of enzymatic reaction, and then, placing reactants under an ultraviolet / visible light analyzer to detect the rising degree of the light absorbancy in the position with the main wave length of 340 nm, so the concentration of the homocysteine can be measured and calculated.

The present invention relates to a method for producing coenzyme Q10 using an enzyme engineering method, using coenzyme Q0 and decapolyisoprene as raw materials, or using coenzyme Q1 and solanesol as raw materials, wherein Phosphorylation of polyisoprene, bromination of solanesol; under the action of polymerase, under the condition of 40 ℃ ~ 60 ℃, the synthesis reaction of coenzyme Q10 is carried out in the reactor, followed by extraction, concentration and crystallization , and dried to obtain the finished product. The polymerase is obtained through the following steps: selecting Rhodopseudomonas photosynthetic bacteria containing coenzyme Q10 and culturing; breaking the bacterial cells and precipitating with 3% saline; separating the precipitate to obtain the polymerase. The invention has specificity, improves the reaction efficiency and improves the product quality. Coenzyme Q10 produced by the enzyme engineering method has no isoprene with cis structure in its side chain, and the biological activity of the product is the same as that of the product produced by the microbial fermentation method, and the production cost is greatly reduced.

The present invention is a composition and methods of administering the composition to improve a person's mental and physical health by increasing energy levels, motivation, willingness to communicate, appetite, and memory. The composition may be used to treat mental conditions by improving a person's wiliness and ability to communicate. The increase in appetite effect of the composition may be used to treat eating disorders. The composition comprises a carbohydrate, one or more NAD+ / NADP+ producing vitamins, poly-sulfurous chelating agents, vasodilating salts, coenzyme-A producing vitamins, antioxidants, kinins, and biological energy sources.

The invention relates to a homocysteine diagnosing / measuring reagent (kit) using the technologies of an enzyme colorimetric method and an enzyme linkage method. At the same time, the invention also relates to a homocysteine concentration measuring method, the reagent composition and reagent ingredients, which belong to the technical field of medical detection and measurement. The reagent (kit) mainly comprises the following ingredients: a buffer solution, cozymase, glutathione disulfide, cozymase A-glutathione, glutathione homocysteine transhydrogenase, glutathione-cozymase A-glutathione transhydrogenase, cozymase A-disulfide reductase and stabilizing agents. The method has the following steps: mixing samples and the reagent by the certain volume percent for making the samples and the reagent take a series of enzymatic reaction, and then, placing reactants under an ultraviolet / visible light analyzer to detect the rising degree of the light absorbancy in the position with the main wave length of 340 nm, so the concentration of the homocysteine can be measured and calculated.

The present invention is a composition and methods of administering the composition to improve a person's mental and physical health by increasing energy levels, motivation, willingness to communicate, appetite, and memory. The composition may be used to treat mental conditions by improving a person's wiliness and ability to communicate. The increase in appetite effect of the composition may be used to treat eating disorders. The composition comprises a carbohydrate, one or more NAD+ / NADP+ producing vitamins, poly-sulfurous chelating agents, vasodilating salts, coenzyme-A producing vitamins, antioxidants, kinins, and biological energy sources.

The invention relates to a homocysteine diagnosing / measuring reagent (kit) using the technologies of an enzyme colorimetric method and an enzyme linkage method. At the same time, the invention also relates to a homocysteine concentration measuring method, the reagent composition and reagent ingredients, which belong to the technical field of medical detection and measurement. The reagent (kit) mainly comprises the following ingredients: a buffer solution, cozymase, glutathione disulfide, cozymase A-glutathione, pyruvic acid, glutathione homocysteine transhydrogenase, glutathione-cozymase A-glutathione transhydrogenase, pyruvate dehydrogenase and stabilizing agents. The method has the following steps: mixing samples and the reagent by the certain volume percent for making the samples and the reagent take a series of enzymatic reaction, and then, placing reactants under an ultraviolet / visible light analyzer to detect the rising degree of the light absorbancy in the position with the main wave length of 340 nm, so the concentration of the homocysteine can be measured and calculated.

The invention relates to a homocysteine diagnosing / measuring reagent (kit) using the technologies of an enzyme colorimetric method and an enzyme linkage method. At the same time, the invention also relates to a homocysteine concentration measuring method, the reagent composition and reagent ingredients, which belong to the technical field of medical detection and measurement. The reagent (kit) mainly comprises the following ingredients: a buffer solution, cozymase, glutathione disulfide, cozymase A-glutathione, 3-formyl acetic acid, glutathione homocysteine transhydrogenase, glutathione-cozymase A-glutathione transhydrogenase, malonate-semialdehyde dehydrogenase and stabilizing agents. The method has the following steps: mixing samples and the reagent by the certain volume percent for making the samples and the reagent take a series of enzymatic reaction, and then, placing reactants under an ultraviolet / visible light analyzer to detect the rising degree of the light absorbancy in the position with the main wave length of 340 nm, so the concentration of the homocysteine can be measured and calculated.