142 results about "Anabolism" patented technology

The invention provides a group of relevant enzymes for preparing mannitol by performing anabolism on Chinese caterpillar fungus serving as a multifunctional production fungus and hirsutella sinensis based on glucose, a gene for encoding these enzymes and application thereof. The relevant enzymes include (1) hexokinase: manA1-A6 proteins of which the sequences are SEQ ID No.1-6, (2) phosphoglucoisomerase: manB1-B3 proteins of which the sequences are SEQ ID No.7-9, and (3) mannitol-1-P dehydrogenase: manC protein of which the sequence is SEQ ID No.10. In the invention, detailed researches are performed on the metabolic pathway of mannitol synthesized by using Chinese caterpillar fungus serving as a multifunctional production fungus, hirsutella sinensis and glucose on the aspect of principle, cloned DNA (Deoxyribose Nucleic Acid) comprising a nucleotide sequence provided by the invention can be transferred into engineering bacteria with transduction, conversion and conjugal transfer methods, and host mannitol is endowed with high expression by regulating the expression of a biosynthetic gene of the mannitol.

Nutritional compositions are provided containing at least one PPAR agonist, at least one PGC-1alpha agonist, and at least one creatine derivative. Methods of promoting anabolism and improving or enhancing physical performance using the compositions are provided. The compositions and methods can be combined with physical training or exercise.

Vegetarian amino acid sources are combined to create a protein blend that closely matches human muscle tissue in terms of amino acid composition. “Closely matching” means that the amino acid composition of the protein blend is within five percent (5%) of the amino acid composition of human muscle for all of the amino acids of the human muscle. The protein blend can be used as food or food supplement to facilitate and bolster muscle anabolism for healthy humans desiring such result and also to treat and or ameliorate the effects of certain diseases and conditions such as sarcopenia, cancer cachexia, HIV wasting, malnutrition, primary muscle diseases (myopathy) and side effects of corticosteroid therapy.

The invention discloses a corynebacterium glutamicum fermentation culture medium for producing L-isoleucine. The fermentation culture medium comprises a basic culture medium and a growth factor, wherein the growth factor is prepared from choline, betaine and vitamin B6; the content of the choline in the fermentation culture medium is 0.2-1g / L, the content of the betaine in the fermentation culture medium is 0.25-0.5mg / L, and the content of vitamin B6 in the fermentation culture medium is 0.05-0.3mg / L. The fermentation culture medium provides the growth factor for corynebacterium glutamicum, maintains osmotic pressure inside and outside the cells of the corynebacterium glutamicum to be balanced, and promotes the anabolism of amino acid, thus improving the yield and sugar-acid conversion rate of the L-isoleucine.

The invention discloses a fermentation production method based on epothilone B metabolic pathways and belongs to the technical field of fermentation engineering. The method comprises the following steps of: 1) inoculating Sorangium cellulosum into an M26 culture medium, carrying out shake cultivation to obtain a seed solution; 2) inoculating the seed solution into fermentation liquor containing resin, carrying out shake culturing for 72-120 hours at 25-35 DEG C, adding a precursor and small molecular substances into the fermentation liquor containing resin; and 3) filtering the fermentation liquor after the culture is finished, collecting the resin, washing the resin and oscillating and extracting with methyl alcohol to obtain extract liquor containing the epothilone B. According to the invention, the precursor and small molecular substances related to the biosynthetic pathways obtained by screening are added into the fermentation liquor, and the constructive metabolism pathways of the epothilone B are disturbed, so that the excellent bacterial strain gives full play to the ability of anabolism and the fermentation level of the epothilone B is improved greatly, thus the fermentation cost is reduced and the commercial process of the anti-cancer drug is promoted.

The invention relates to a rhodotorula glutinis oil genetic engineering strain and a construction method and an application thereof. The construction method of the genetic engineering strain is mainly as follows: utilizing rDNA (recombinant deoxyribonucleic acid) of rhodotorula glutinis as a target sequence for homologous integration, using strong promoter genes PGK1 of saccharomyces cerevisiae and malate dehydrogenase genes ME of chaetomium cochloides to construct an expression vector to be introduced into rhodotorula glutinis, and enabling ME genes to obtain high-efficient expression in a rhodotorula glutinis body, wherein the content of lipid in a transformant is improved by 2.5 times in comparison with a wild strain. According to the construction method disclosed by the invention, key enzyme genes and a strong promoter for anabolism of the lipid are introduced on the basis that the anabolism of microbial oil is known, so that the lipid metabolism is regulated and controlled, and the yield of oil is improved. The genetic engineering strain can be applied to production of the microbial oil and development of functional oil related products, such as medicaments, health care products and the like.

The invention belongs to the technical field of cosmetics, and particularly relates to a composition with a seven-whitening effect as well as a preparation method and an application of the composition. The composition with the seven whitening effects comprises the following components in parts by mass: 8-12 parts of an antioxidant, 2-5 parts of a melanin synthesis inhibitor, 1-3 parts of an enzymeinhibitor, 1-2 parts of a melanin polymer inhibitor, 4-7 parts of a melanin reducing agent, 2-4 parts of a melanin transfer inhibitor and 3-5 parts of a metabolic promoter. The composition with the seven whitening effects provided by the invention complies with skin physiology knowledge and a melanin anabolism process path, the whitening problem is solved through a reasonable and scientific skinphysiological structure, and the whitening effect is remarkable.

It has now been discovered that GLP-1 treatment after acute stroke or hemorrhage, preferably intravenous administration, can be an ideal treatment because it provides a means for optimizing insulin secretion, increasing brain anabolism, enhancing insulin effectiveness by suppressing glucagon, and maintaining euglycemia or mild hypoglycemia with no risk of severe hypoglycemia.

The invention provides a preserving fluid and a preserving method for maintaining high cell activity of a tissue sample, wherein the preserving fluid comprises the components: ion buffer solution components, carbohydrate components,, components avoiding the generation of ice crystals in and out solutions of tissue cells at a low temperature of 0-6 DEG C, components providing supplement, antioxidant and anti-apoptosis components for anabolism of the tissue cells; the preserving fluid has good biocompatibility, does not contain toxic or harmful components, and also does not contain components such as animal-derived protein, antibiotics and hormones influencing tissue gene expression. Different from traditional ultralow-temperature cryopreservation (-80 DEG C), the cryopreservation method disclosed by the invention can effectively realize low-temperature storage (0-6 DEG C) of fresh tissue samples of human beings and animals, maintains high cell activity of tissues, maintains tissue morphology, and is used for preservation and transportation of the tissue samples. Meanwhile, the nucleic acid integrity of the tissue and the stability of gene expression can be effectively maintained, the original state of the tissue is maintained, and the method can be used for gene detection, scientific research and other related experiments.

The invention discloses a cytochrome P450 gene which participates in anabolism of tanshinone compound as well as coding product and application thereof, and the invention belongs to the field of medicinal plant gene engineering. The gene is obtained by clone from red sage root for the first time, and is a key enzyme gene in anabolism pathway of tanshinone compound, and can catalyze ferruginol into 11-hydroxy ferruginol. The CYP76AH3 gene provided by the invention has a nucleotide sequence shown in the SEQ ID No.1. The gene coding protein comprises an amino acid residue sequence shown in the SEQ ID No.2 of the sequence table and a protein derived from the SEQ ID No.2 whose activity is identical to that of the amino acid residue sequence shown in the SEQ ID No.2. The CYP76AH3 gene provided by the invention is closely related to anabolism of tanshinone compound, and has important theoretical and real meanings for regulating plant diterpenoids compounds and increasing contents of diterpene active component tanshinone in red sage root by biological technology.

The invention relates to a method for producing epsilon-poly-L-lysine. The method is characterized in that a medium containing 1-10g / L of glycine is added at an early or middle stage of culture. By adding glycine in a fermentation process in the invention, glycine which enters a folic acid metabolism approach allows sufficient one-carbon groups to be supplied to a biosynthesis process, the anabolism activity of bacterial strains to be improved, the synthesis of a precursor L-lysine and epsilon-poly-L-lysine to be increased, and the accumulation amount of epsilon-poly-L-lysine to be 20-50% higher than the accumulation amount obtained through original technologies.

Belonging to the field of gene engineering, the invention relates to an AP2 / EREBP transcription factor bNwrI1 gene and an application thereof in adjusting plant seed photosynthesis and oil and fat anabolism. The transcription factor is extracted from brassica napus and is one of the following polypeptides: a polypeptide provided with an amino acid sequence as SEQ ID NO:2 indicates, a polypeptide which is formed with the amino acid sequence replaced, deleted and added by one or more than one amino acid residue and provided with the amino acid sequence and a polypeptide with functions thereof derivating from the polypeptide provided with the amino acid sequence. The transcription factor and coding genes thereof enjoy extensive application value in plant culturing field and can enable the plant to coordinately regulate photosynthesis and fatty acid anabolism, improves seed photosynthesis and oil and fat accumulation capacity, thus improving plant yield and oil content as well as correlated characteristics.

The invention provides a synthetic medium for culturing a phellinus igniarius liquid. The synthetic medium takes glucose as a carbon source and 20 kinds of amino acid as a nitrogen source, and consists of inorganic salt and vitamin. The ratio of carbon to nitrogen is designed as 50 to 1; and the specific concentration g / l is as follows: the glucose: 30 to 80 grams; glutamic acid: 0.1 to 1.5 grams; glutamine: 0.1 to 1.5 grams; aspartic acid: 0.1 to 1.5 grams; asparagines: 0.1 to 1.5 grams; glutamic acid: 0.01 to 0.1 gram; tryptophan: 0.01 to 0.1 gram; valine: 0.01 to 0.1 gram; alanine: 0.01 to 0.1 gram; etc. During the whole culture process of the synthetic medium, phellinus igniarius mycelium is quick in growth; the yield of the phellinus igniarius mycelium can reach 20 grams per liter to the highest degree; simultaneously no agricultural and sideline product is introduced as the carbon source and the nitrogen source; substances such as proteins in the medium, medium compositions and so on are not carried; phellinus igniarius exopolysaccharide produced through fermentation has high purity and stable quality; and the yield of phellinus igniarius polysaccharide can reach 0.198 gram per liter to the highest degree. The synthetic medium has the advantages of clear compositions, stable quality and so on, and can be used for mass culture of the phellinus igniarius liquid and research of the regulation and control rule of polysaccharide anabolism.

The invention discloses an analogy method based on an activated sludge purification process of a HPP lattice gas cellular automaton model, comprising the following steps: setting up a corresponding initial model in an activated sludge system and initializing a cellular state; setting a boundary point; and ensuring an evolution rule according to the initial model. The method comprises the reaction process of particles; and the reaction process comprises particle absorption, particle catabolism, particle anabolism and endogenous respiration. Based on an artificial life system and the HPP lattice gas cellular automaton model, the invention designs the evolution rule of an activated sludge cell automaton and realizes the evolution, simulates a circumstance that microorganism absorbs and decomposes organic matters, well simulates the logarithmic phase, the decelerating grow phase and the endogenous respiration phase of an activated sludge growth model curve, well simulates the degradation curve of BOD organic matters, and has a directive function for further recognizing and understanding the saprobia settlement process.

The invention relates to a method for applying establishment of gene recombination mobile fermentation monad to alcoholic fermentation. The microorganism is obtained by leading an operon capable of expressing in the mobile fermentation monad, wherein the operon consists of a sequence for coding a promoter and a sequence for coding a structure gene and comprises a sequence coding at least one promoter, and the sequence of the promoter can be identified by the mobile fermentation monad and regulates expression of at least more than one structural gene. The operon comprises a sequence for coding at least one heat shock protein HSP and is used for enhancing the survivability of the mobile fermentation monad in an adverse environment and increasing the temperature of alcoholic fermentation; the operon comprises a sequence for coding at least one lysine anabolism enzyme yfdz so that the mobile fermentation monad can automatically synthesize lysine; the operon comprises a sequence for coding at least one methionine anabolism enzyme metB so that the mobile fermentation monad can automatically synthesize methionine used for lowering the nutrition requirement of the mobile fermentation monad for cultures; and the operon also comprises a sequence for coding a structural gene, which ensures that the mobile fermentation monad can utilize at least one pentaose to generate. The operon enters the mobile fermentation monad in a plasmid transforming way or integrated with the genome of the mobile fermentation monad to obtain a transporton recombinant in a transporton way. The mobile fermentation monad obtained by gene engineering enhances the survivability in an adverse environment, can be fermented to generate the alcohol under higher temperature, obviously lowers the nutrition requirement for the cultures, can utilize the pentaoses to generate the alcohol and still stably retains the characteristics after transfer of culture for 200 times.

The invention belongs to the technical field of biological materials, and particularly relates to a self-crosslinking hyaluronic acid and gelatin composite hydrogel injection, and a preparation methodand application of the self-crosslinking hyaluronic acid and gelatin composite hydrogel injection. The structure of the self-crosslinking hyaluronic acid and gelatin composite hydrogel injection is shown as a formula I. According to the preparation method, the two sulfydryl modified natural materials are prepared through a spontaneous oxidation reaction for forming disulfide bonds. The self-crosslinking hyaluronic acid and gelatin composite hydrogel injection provided by the invention can be used as a tissue engineering scaffold or / and other biological material loaded cells, drugs and bioactive substances. The preparation method is scientific in design and is simple and convenient to operate, and the prepared hydrogel injection has good hydrophilicity, biocompatibility and degradability,can well support normal form and proliferation behaviors of cells and promote anabolism of the cells, and shows an application prospect in tissue engineering and regenerative medicine.

This invention discloses a kind of genetic engineering bacteria with enhanced tryptophan anabolism, and the recombinant plasmid contained in the genetic engineering bacteria. The recombinant plasmid is prepared by: connecting tryptophan synthetase gene trpBA and phosphoglycerate dehydrogenase gene mutant M3serA gene in series to obtain M3BAT-I, a kind of genetic engineering bacteria with enhanced tryptophan anabolism, expressing induced by IPTG, and analyzing with SDS-PAGE to express tryptophan synthetase beta subunit, phosphoglycerate dehydrogenase M3 mutant, and tryptophan synthetaselpha subunit. After culture of the genetic engineering bacteria with enhanced tryptophan anabolism (tnaA gene removed), the tryptophan content in the supernatant is increased from 2 mg / L to 99 mg / L.

The invention discloses a production and fermentation process of coenzyme Q10. On the premise that the fermentation quality of the coenzyme Q10 is retained, according to the synthesis rate of the main byproduct 5-demethoxylation coenzyme Q10 in the bacteria metabolism process of the coenzyme Q10 as well as the content change thereof, the feedback adjustment is performed on the initial reaction of metabolism, and the speed restriction action is acted on the anabolism, therefore, the feedback adjustment is performed on the accumulation of the product coenzyme Q10 in the cell, the fermenting level of the coenzyme Q10 is greatly promoted through the optimization to the concentration of the main substrate in the fermenting formula and through the optimization control to the stirring rotational speed during the fermenting process and can be improved to above 3000 u / ml, the determination of the optimal time for pot laying improves the utilization ratio of time of the production of the coenzyme Q10 in the cell, the fermentation cost is reduced, and the consumption of the fermenting raw material is greatly reduced.

The invention provides a salmon and trout feed based on a peroxisome proliferator activated receptor (PPAR) and a method for preparing the salmon and trout feed. The salmon and trout feed comprises proteins, fat, trace elements and other basic substances which are required by the salmon and trout growth, and further contains natural PPAR alpha and / or PPAR beta binding active substances, such as daidzin, curcumin, isoflavone, arachidonic acid, berberine, naringenin, polydatin, kaempferol, isoflavoues aglycone, conjugated linoleic acid and the like. The salmon and trout feed can regulate the fat and fatty acid anabolism of the cultured salmon and trout, the fat content of the salmon and trout is lowered, the beneficial omega-3 fatty acid content is improved, and the salmon and trout quality is improved.

The present invention provides a set of cordycepin anabolism-related enzymes from bering producing strain cordyceps sinensis hirsutella sinensis-participated ADP ribose starting, genes encoding the enzymes, and application thereof. The related enzymes include proteins shown as SEQ ID No. 1-17, and the coding genes correspond to nucleotide sequences shown as SEQ ID No. 18-34. The invention conducts detailed researches on the principle of metabolic pathways of cordycepin synthesis from bering producing strain cordyceps sinensis hirsutella sinensis ADP ribose. The cloned DNA of the nucleotide sequences provided by the invention can be transferred into engineering bacteria by a transduction, transformation and conjugal transfer method. By adjusting the expression of cordycepin biosynthesis genes, high expression of cordycepin for the host is given.

The invention discloses a preparation method of quick-dissolving branched-chain amino acid in the technical field of nutritional supplements. The preparation method comprises the steps that powder of leucine, isoleucine and valine in any proportions is taken as raw materials to be mixed, and the powder particle size ranges from 60 mesh to 100 mesh; water or an ethanol-water solution accounting for 0.4% to 0.6% of the weight of the raw materials is taken, a mixture of granulesten, xylitol, glycerol and dextrin is added into the water or the ethanol-water solution, and the materials are fully mixed to be uniform to serve as a spraying agent, wherein the weight of the mixture accounts for 0.3% to 1% of the total weight of the raw materials; the spraying agent is uniformly sprayed to wrap the surface of the raw materials which are continuously stirred through a spraying device, and then the quick-dissolving branched-chain amino acid product is obtained. The product prepared through the method can be quickly dissolved in water, and liquid obtained through dissolving is kept clarified; meanwhile, drying is not needed in the processing process. The product prepared through the method can serve as a nutritional supplement for allowing people to eat, is good in mouthfeel and convenient to eat and has the effect of promoting anabolism.

The invention discloses a composite amino acid vitamin injection and the application thereof. Every 1000ml of the composite amino acid vitamin injection comprises 5-40g of L-leucine, 2-30g of L-isoleucine, 2-30g of L-valine, 5-90g of L-alanyl-L-glutamine, 1-5g of L-threonin 1-10g of L-lysine, 1-12g of L-methionine, 1-10g of L-phenylalanine, 0.5-8g of L-tryptophan, 2-30g of L-arginine, 1-10g of L-histidine, 1-100mg of vitamin B6, 0-50mg of vitamin B1 and 0-100mu g of vitamin B12. Aiming at variation of stress, immunity, metabolism and the like of a human body in a perioperative anesthesia period, the composite amino acid vitamin injection achieves the purposes of inhibiting catabolism, promoting anabolism, generating heat and keeping warm, improving body substance energy metabolism and immunity functions and accelerating heal through creative formulae on the basis of enhanced recovery after surgery.

The invention relates to a method for producing nervonic acid by utilizing transgenic microalgae. The method comprises the steps that the gene of a key enzyme, namely 3-ketoacyl CoA (coenzyme A) synthase, with high nervonic acid production capacity of fatty acid prolonged anabolism pathway in organism is transferred to a host microalgae cell, so that the microalgae is endowed with or enhanced in the nervonic acid synthesis capability; by cultivating the transgenic microalgae and promoting oil and fat accumulation of the microalgae, oil and fat with high content of nervonic acid can be extracted from the cultivated microalgae cells, and further, nervonic acid can be extracted from the oil and fat.

The invention relates to a recirculating prawn aquaculture method in combination with a nitrification-assimilation effect and belongs to the technical field of aquaculture. A culture system used in the method comprises a culture pond, a microfilter, a water circulating pump, a biofilter, an ultraviolet sterilization device and a Roots blower; when the culture system is utilized for culture, browngranulated sugar 40-50% of the bait feeding amount or molasses 50-60% of the bait feeding amount is added into the culture pond every day to promote growth of heterotrophic bacteria, and part of metabolic waste is removed by utilizing the bacterial assimilation effect. According to the method, in the circulating water culture system with nitrification as a water treatment core, a carbon source iscontinuously increased in the culture process, growth of the rhodobacteriaceae bacteria in a culture water body is promoted, the bacterial assimilation effect is strengthened, meanwhile, the nitrification effect of nitrification bacteria is not inhibited, the assimilation effect and the nitrification effect jointly purify the water body, the microbial water treatment efficiency is improved, the load of a water treatment system is reduced, and a culture water environment is improved.

The invention relates to a promoting agent for promoting tobacco mature yellowing and a promoting method thereof. The promoting agent comprises the following ingredients in percentage by weight: 0.05 to 0.25 percent of glufosinate, 0.5 to 1.5 percent of potassium bicarbonate and the balance water. The promoting method comprises the following steps that in the tobacco mature period of 7 to 10 days before the tobacco harvesting, the promoting agent is sprayed onto leaves of tobacco leaves until the surfaces of the tobacco leaves are wet. In the mature period of the tobacco, the promoting agent capable of accelerating the tobacco mature yellowing is sprayed onto the leaves of tobacco plants, and the activity of nitratases and GS enzymes in the nitrogen element metabolic process is inhibited through the promoting agents, so that the nitrogen element anabolism is decelerated, the catabolism is accelerated, and the degradation of pigment in the tobacco leaves and the macromolecular substances in the tobacco leaves is accelerated, so that the tobacco leaves naturally realize mature yellowing, and meanwhile, the mature degree of the tobacco leaves is also ensured after the sufficient degradation of pigment and macromolecular substances.

The invention provides a strawberry NCED gene and a construction of a vector thereof. The nucleotide sequence of the gene is shown in SEQ ID NO:1 or has a cDNA sequence which has more than 90% of homology with the nucleotide sequence shown in SEQ ID NO:1 and is used to code the same functional protein. In the invention, the coding gene of the 9-cis-epoxy carotene dioxygenase is cloned from strawberry, the gene has critical regulating action on the synthesis of ABA in the processes of maturity and wilting or the conditions of drought, high temperature and low temperature. The invention lays the foundation for the research of the strawberry ABA anabolism mechanism and provides a gene source for the genetic engineering regulation of the metabolism and accumulation of ABA in plant.

The invention discloses a method for screening control enzyme of microalgae adipopexis. The method comprises the steps of (1) establishing a salt environment under the manual simulation of a laboratory, and culturing salt-sensitive chlorella and salt-resistant chlorella; (2) analyzing fluorescence difference gel electrophoresis proteomics of the whole protein of algae cells; and (3) screening and identifying key control enzyme of the microalgae adipopexis. The method disclosed by the invention has the advantages that due to the same key control enzyme for inducing grease synthesis in different algae plants by adopting the salt-resistant chlorella and the salt-sensitive chlorella as screening objects under the salt environment, the effective control for anabolism of microalgae grease with different energies and the construction of high-yield grease gene engineering microalgae are excepted to be realized; the error between gel and gel in the traditional two-dimensional electrophoresis (silver staining and coomassie-brilliant-blue staining) is eliminated, and the accuracy and the repeatability are improved; and simultaneously, an established general technology system for discovering and identifying the key control enzyme of the grease synthesis can be applied to the fields of metabonomics analysis of animals, plants, microorganisms and fungus in a radiation manner.

The invention discloses a compound bacterium agent capable of improving the content of starch and dry substances in potatoes and application of the compound bacterium agent. The compound bacterium agent is composed of lactobacillus plantarum P-8 and lactobacillus casei Zhang. The two bacteria have obvious influences on growth and anabolism of the potatoes; particularly, the content of the starch and the dry substances in the potatoes can be improved, so that relatively ideal raw materials are provided for preparation of potato starch and other high-additional-value products; and meanwhile, the storage period of the potatoes is prolonged.