The invention discloses a gynecological lotion and a preparation method thereof. The gynecological lotion contains the active ingredients of raw materials by weight parts: 2-3 of cloves, 3-5 of pepper, 10-15 of cnidium, 3-5 of mint, 10-15 of salvia, 7-9 of radixstemonae, 10-15 of smilax, 5-8 of white moss leather, 7-9 of sophora, 4-6 of Phellodendron, 3-5 of polygonum cuspidate, 4-6 of dandelion, 3-5 of berberine, 10-15 of fructuskochiae and 0.1-0.3 of borneol. The novel gynecological lotion consists of over ten Chinese herbs, has reasonable component prescription compatibility, can obviously reduce the number of vaginal disease-causing micro-organisms and the infection chances, and has more prominent effects of bacterial, fungal and trichomonas vaginitis caused by common micro-organisms.