38 results about "Jatrorrhizine" patented technology

The invention discloses berberine represented by structural formula (I), coptisine represented by structural formula (II) or their active metabolites, and pharmaceutically acceptable salts thereof for the preparation of prevention and / or treatment of uric acid The application in nephropathy (gout nephropathy) medicine, and the active metabolite of berberine as shown in structural formula (III), (IV), (V), (VI), (VII), (VIII), (IX) comprises Jatrorrhizine, dihydroberberine, tangsongcaofen, berbererythrine, demethylene berberine, palmatine, and African tetrandrine.

The invention discloses a pharmaceutical composition for preventing and treating rheumatoid arthritis. The traditional Chinese medicine raw materials for constituting active ingredients of the pharmaceutical composition by weight are as follows: 5.5-6.0mg of berberine, 0.3-0.4mg of palmatine, 1.5-2.0mg of jatrorrhizine, 1.5-1.8mg of magnoflorine, 0.1-0.2mg of phellodendrine, 0.2-0.3mg of coptisine, 55-60mg of baicalin, 1.5-2.0mg of baicalein, 5.0-7.0mg of wogonoside, 0.03-0.05mg of wogonin, 20-25mg of geniposide, 0.1-0.2mg of crocin and 0.25-0.3mg of chlorogenic acid. The results of pharmacological tests show that the pharmaceutical composition can significantly reduce the disease severity of CIA mice with the rheumatoid arthritis, significantly improve the inflammation and the immune injury situation at lesion sites in comparison with a control group and be used for preparing drugs for preventing and treating the rheumatoid arthritis.

The invention discloses a coptis alkaloid and ferulic acid compound with glucose-lowering activity, which comprises the following components in percentage by weight: 0.1 to 99.9 percent of coptis alkaloid and 0.1 to 99.9 percent of ferulic acid, wherein the coptis alkaloid contains berberine or coptisine or palmatine or jatrorrhizine or the sum of four coptis alkaloids, or is total coptis alkaloids extracted from coptis. The compound has the advantages of better glucose-lowering effect compared with that of the coptis alkaloid or ferulic acid, and obvious glucose-lowering effect according to administration in a dosage of 0.5 to 5 grams every person per day.

The invention relates to a jatrorrhizine platinum (II) complex, a synthesis method thereof and an application of the complex in preparing antitumor drugs. The synthesis method of the jatrorrhizine platinum (II) complex comprises the following steps: (1) dissolving jatrorrhizine bromide derivatives, potassium chloride and lutidine amine in a solvent, and reacting at 90°C to obtain Yellow solid powder is Jat ligand; (2) Jat ligand prepared in step (1) and dichlorobis(dimethylsulfoxide) platinum (II) are dissolved in acetonitrile solution at 55-65 The coordination reaction is carried out at ℃; (3) the product is washed and dried to obtain the target product. For the first time, the present invention uses the jatrorrhizine derivative Jat as an active ligand to synthesize its platinum complex, which exhibits superior in vivo and in vitro anti-tumor activity and targeting, has potential medicinal value, and is expected to be used in various anti-tumor drugs preparation. The structural formula of the jatrorrhizine platinum (II) complex is as follows:

The invention discloses application of jatrorrhizine hydrochloride in preparation of a drug for preventing and treating influenza, and belongs to the field of traditional Chinese medicine application. The application is implemented by the following technical scheme: firstly incubating influenza virus neuraminidase liquid from different subtypes and jatrorrhizine hydrochloride in an incubator at 37 DEG C for 30 minutes, adding a fluorogenic substrate buffer solution to the incubator to be detected, finding out the situation that jatrorrhizine hydrochloride can play an effective role on H1N1, H3N2 and influenza virus B, detecting anti-influenza action of jatrorrhizine hydrochloride on a cellular level, finding out the situation that jatrorrhizine hydrochloride can treat the cells infected H1N1 and H3N2, and at last detecting the influence of jatrorrhizine hydrochloride on a mouse influenza model. The study shows that when jatrorrhizine hydrochloride is added, the mouse weight reducing degree of the influenza infected mice can be obviously inhibited; jatrorrhizine hydrochloride can enable the mice to gradually recover to the health level and obviously lower the lung index of the influenza infected mice.

The invention relates to a method for extracting alkaloids in Coptidis Rhizoma, comprising the following steps: pulverizing Coptidis Rhizoma medicinal materials, extracting in a water bath, filtering, and purifying the filtrate with acid precipitation or column chromatography to obtain the alkaloids; the alkaloids include Coptis Berberine, berberine, palmatine, and jatrorrhizine. The extraction method provided by the invention can obtain a high content of alkaloids, which is convenient for subsequent analysis and precise purification.

The invention discloses a method for separating alkaloid compounds in eucalyptus by refining countercurrent chromatography in a pH-zone. The conditions used in the separation are: the solvent system is chloroform:methanol:water=3.5-4.5:2.5- 3.5: 2.5-3.5, pH-zone refined countercurrent chromatography column volume is 200-400, sample loading is 2-5g, rotation speed is 300-1000rpm, upper phase is stationary phase, lower phase is mobile phase, flow rate is 1.5-2.5 mL / min, the stationary phase retention rate is 35.71%, and the detection wavelength is 250-260nm. The preparation cost of the method of the present invention is lower than that of the prior art, and the operation is simple and efficient, and it can separate and prepare jatrorrhizine, berberine, palmatine, and African fangji with a purity greater than 96% from the traditional Chinese medicine Gongluo in large batches. The separation and preparation method of alkali monomer compound, the invention firstly separates tetrandrine from the traditional Chinese medicine Gongluomu.

The present invention discloses a method for separating alkaloid compounds in eucalyptus by using high-speed countercurrent chromatography. The conditions used for the separation are: the solvent system is chloroform:methanol:0.5mol L-1 hydrochloric acid solution=3.5-4.5:1- 2: 1.5-2.5, the column volume of high-speed countercurrent chromatography is 200-400, the sample volume is 200-500, the rotation speed is 300-1000rpm, the upper phase is the stationary phase, the lower phase is the mobile phase, the flow rate is 1.5-2.5 mL / min, and the detection Wavelength 250-260nm. The preparation cost of the method of the present invention is lower than that of the prior art, and the operation is simple and efficient, and it can separate and prepare jatrorrhizine, berberine, palmatine, and African fangji with a purity greater than 96% from the traditional Chinese medicine Gongluo in large batches. The separation and preparation method of alkali monomer compound, the invention firstly separates tetrandrine from the traditional Chinese medicine Gongluomu.

The invention relates to the technical field of biomedicine, in particular to the application of jatrorrhizine hydrochloride in reversing the drug resistance of pancreatic cancer cells. Under the synergistic effect of said jatrorrhizine hydrochloride, gemcitabine has a more significant Reversal of the resistance of pancreatic cancer cells to gemcitabine. The present invention studies pancreatic cancer Panc-1 cells and gemcitabine drug-resistant Panc-1 cells, and finds that under the action of jatrorrhizine hydrochloride, the drug sensitivity of pancreatic cancer cells to gemcitabine is enhanced, and low concentrations of gemcitabine and jatrorrhizine hydrochloride Combined use can achieve the effect of high concentration gemcitabine on drug-resistant Panc‑1 cells, and as the concentration of jatrorrhizine hydrochloride increases, the combination with gemcitabine can effectively inhibit the growth of drug-resistant Panc‑1 cells, and its growth inhibitory effect is much higher The drug resistance of drug-resistant Panc‑1 cells to gemcitabine was significantly reversed when gemcitabine was used alone.

The invention provides medicine root berberrubine derived from an amur cork-tree processed product and a separation preparation method and application thereof. The method includes the steps that 1, jatrorrhizine hydrochloride reference substances are precisely weighed, placed in an evaporation dish to be baked, taken out and cooled, chromatographic grade methyl alcohol is added, millipore filter filtering is conducted, and a baked jatrorrhizine reference substance solution is obtained; 2, the baked jatrorrhizine reference substance solution is separated through a preparative liquid phase, eluant of a target chromatographic peak is collected and purified through a sephadex column, the purified eluant is subjected to nitrogen blowing and freeze drying, and pink powder, namely, medicine root berberrubine crystals prepared through separation are obtained. The prepared medicine root berberrubine is a new compound found in amur cork-tree processed decoction pieces, the separation preparation method has the advantages of being high in efficiency, stable in process, easy and convenient to operate and low in cost, high-purity separation preparation of a large number of medicine root berberrubine monomers can be achieved, and the medicine root berberrubine compound has good macrophage phagocytosis and high immunization.