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Method for separating and purifying coptis alkaloids by utilizing polyamide resin

A technology of polyamide resin and Coptis chinensis, which is applied in the field of natural medicine, can solve the problems of expensive instruments and equipment, less sample loading, complicated operation, etc., and achieve the effects of improving safety and economic benefits, reducing production costs, and simple process operation.

Inactive Publication Date: 2011-09-07
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is also a method (Journal of Paharmacy and Pharmacology, 2005, 57: 367-374.) firstly through extraction, then repeated column chromatography such as Dia-ion, MCI and ODS, and finally recrystallization to obtain berberine, coptisine and palmatine , table berberine, jatrorrhizine and Groenlandicine and other 6 alkaloid monomers, this method is also cumbersome to operate, wastes time, and has low economic benefits
[0005] Compared with traditional methods, the method of countercurrent separation of Coptidis alkaloids (separation and purification technology, 2011, 76: 428-431.) greatly improves the efficiency. It is difficult to expand the application, the economic benefit is not high, and the equipment is expensive, so the separation of coptis alkaloids by countercurrent also has certain limitations for the promotion of industrialization
[0006] Polyamide resin is a commonly used separation material. It has the characteristics of simple equipment, reusability, low energy consumption, and wide application range. It has been widely used in the fields of traditional Chinese medicine and food, especially for the separation of phenols and flavonoids. And its use in the separation of coptis alkaloids has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 30g Coptis rhizome, add 70% ethanol containing 0.5% hydrochloric acid with a weight volume ratio of 5 times and ultrasonically extract it three times, each time for one hour, combine the extracts, and recover the ethanol under reduced pressure with a rotary evaporator to obtain a crude extract. Take 1.5g of crude extract and dissolve it in methanol, then use 1 times the amount of 100-200 mesh polyamide to accompany the sample, evaporate the solvent and load the sample. Take 100-200 mesh polyamide which is 25 times the amount of the crude extract, and put it in a column with a diameter of 2 cm by wet method with dichloromethane to obtain a column layer with a height of 40 cm, and then use a dichloromethane-methanol system to carry out a pressure gradient Elution: wash polyamide resin 25BV (column volume) with dichloromethane first, then wash 8BV with dichloromethane-methanol (100:1), wash 12BV with dichloromethane-methanol (50:1), dichloromethane- Rinse 3BV with met...

Embodiment 2

[0022] Take 30 g of Coptidis rhizome, add methanol containing 1% hydrochloric acid with a weight-to-volume ratio of 5 times, reflux and extract three times, each time for one hour, combine the extracts, and use a rotary evaporator to recover ethanol under reduced pressure to obtain a crude extract. Take 1.5g of crude extract and dissolve it in methanol, then use 0.8 times the amount of 100-200 mesh polyamide to accompany the sample, evaporate the solvent and load the sample. Take 100-200-mesh polyamide that is 20 times the amount of the crude extract, and put it in a column with a diameter of 2 cm by wet method with dichloromethane to obtain a column layer with a height of 31 cm, then wash 83 BV with dichloromethane, and elute The flow rate is 5mL / min, and the fractions are checked by the TLC method. The TLC conditions are the same as those in Example 1, and the same fractions are combined. 112.01mg of palmatine, 341.22mg of berberine, 23.02mg of coptisine, 35.35mg of tetrandr...

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PUM

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Abstract

The invention relates to the natural medicament field, in particular to a method for separating and purifying coptis alkaloids by utilizing polyamide resin. The method is characterized in that polyamide is used to deal with coptis coarse extract, adding the mixture in a polyamide column, dichloromethane is used for elution or the mixed solvent of dichloromethane and methanol is used for gradient elution, the fractions are inspected through thin layer chromatography, the same fractions are combined to separate out seven alkaloids, namely berberine, palmatine, coptisine, columbamine, jatrorrhizine, magnoflorine and groenlandcine. The method has the advantages of simple process operation, low sample loss, higher separation efficiency and lower production cost.

Description

technical field [0001] The invention relates to the field of natural medicines, in particular to a method for separating and purifying coptis alkaloids by using polyamide resin. Background technique [0002] Coptis chinensis is one of the most commonly used traditional Chinese medicines in my country. It is an ancient antibacterial drug. It has been used clinically for antipyretic and analgesic and anti-intestinal bacterial infection for a long time. In recent years, it has been found that Coptidis Rhizoma also has pharmacological effects such as antiarrhythmia, lowering blood fat, lowering blood sugar, anticancer, and antiplatelet aggregation. The main active ingredients of Coptis chinensis are berberine, palmatine, coptisine, epiberberine, tetrandrine, jatrorrhizine, Groenlandcine and magnoline and other isoquinoline alkaloids. Studies have shown that these alkaloids have antibacterial, anti-inflammatory, hypoglycemic, anticancer and other activities. [0003] At present...

Claims

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Application Information

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IPC IPC(8): C07D491/147C07D455/03C07D491/22
Inventor 孔令义杨新苗汪俊松罗建光罗俊
Owner CHINA PHARM UNIV
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