The invention provides an aquatic animal preparation capable of protecting liver. The aquatic animal preparation capable of protecting liver is composed of an A preparation and a B preparation. The A preparation is composed of 70 to 100% of a Chinese herbal medicine mixture, 0 to 15% of a flavonoid compound, 0 to 15% of a saponin compound, 0 to 15% of an alkaloid, 0 to 15% of trace element, and 0 to 15% of a polysaccharide; and the B preparation is one or a mixture of a plurality of ingredients selected from a feed additive, a photosynthesis accelerant, and a vitamin. In vivo, the aquatic animal preparation is capable of increasing the activity of liver cells, repairing cell damage, removing a plurality of toxins in aquatic animals, relieving liver burden, improving immunity, resisting free radical injury, reducing lipid peroxide, protecting liver, nourishing gallbladder, and preventing tumor; beneficial substances of living bacteria agents and Chinese herbal particles dissolved in water are capable of inhibiting planting of pathogenic bacteria in water bodies, and killing virokine.