4496 results about "Alkaloid" patented technology

New thiol and disulfide-containing maytansinoids bearing a mono or di-alkyl substitution on the α-carbon atom bearing the sulfur atom are disclosed. Also disclosed are methods for the synthesis of these new maytansinoids and methods for the linkage of these new maytansinoids to cell-binding agents. The maytansinoid-cell-binding agent conjugates are useful as therapeutic agents, which are delivered specifically to target cells and are cytotoxic. These conjugates display vastly improved therapeutic efficacy in animal tumor models compared to the previously described agents.

A new method and system for desorption ionization is described and applied to the ionization of various compounds, including peptides and proteins present on metal, polymer, and mineral surfaces. Desorption electrospray ionization (DESI) is carried out by directing charged droplets and / or ions of a liquid onto the surface to be analyzed. The impact of the charged particles on the surface produces gaseous ions of material originally present on the surface. The resulting mass spectra are similar to normal ESI mass spectra in that they show mainly singly or multiply charged molecular ions of the analytes. The DESI phenomenon was observed both in the case of conductive and insulator surfaces and for compounds ranging from nonpolar small molecules such as lycopene, the alkaloid coniceine, and small drugs, through polar compounds such as peptides and proteins. Changes in the solution that is sprayed can be used to selectively ionize particular compounds, including those in biological matrices. In vivo analysis is demonstrated.

The invention relates to devices and methods for delivering nicotine and / or other alkaloids from tobacco, other plants and other natural sources. More particularly, the invention relates to devices and methods for delivering an aerosol of nicotine to a user's lungs without combustion of the nicotine source materials.

The present invention provides a process for the preparation and purification of thiol-containing maytansinoids comprising the steps of: (1) reductive hydrolysis of a maytansinoid C-3 ester with a reducing agent selected from the group consisting lithium trimethoxyaluminum hydride (LiAl (OMe)3H), lithium triethoxyaluminum hydride (LiAl(OEt)3H), lithium tripropoxyaluminum hydride (LiAl (OPr)3H), sodium trimethoxyaluminum hydride (NaAl (OMe)3H), sodium triethoxyaluminum hydride (NaAl(OEt)3H) and sodium tripropoxyaluminum hydride (NaAl(OPr)3H) to yield a maytansinol; (2) purifying the maytansinol to remove side products when present; (3) esterifying the purified maytansinol with a carboxylic acid to yield a mixture of an L- and a D-aminoacyl ester of maytansinol; (4) separating the L-aminoacyl ester of maytansinol from the reaction mixture in (3); (5) reducing the L-aminoacyl ester of maytansinol to yield a thiol-containing maytansinoid; and (5) purifying the thiol-containing maytansinoid.

A method for the administration of nicotine, THC, tobacco, cannabidiol or a base alkaloid includes administering in the oral or nasal cavity an absorption conditioning unit having at least two agents selected from the group consisting of (a) a buffer agent, (b) a capturing agent, (c) a penetration agent, and (d) a thermal agent, to the mammal, and then administering by inhalation a bioactive agent selected from the group consisting of nicotine, THC, cannabidiol and a base alkaloid. The absorption conditioning unit may be in a dosage form not containing a drug. The absorption conditioning unit may create a pH in the oral cavity or nasal cavity of 7.8-10 for a period of ten minutes or more after administration, the dosage form not containing an acid and not containing a drug.

A saliva-soluble stimulant unit comprising an active ingredient and optional ingredients comprising flavor and aroma additives incorporated in a gel prepared by gelling a water-binding gelling agent, in which the active ingredient comprises nicotine or other alkaloids with the same direction of activity, said unit having i) a texture profile, determined by texture profile analysis, with parameter values of firmness, hardness, brittleness, adhesiveness, elasticity, and cohesiveness within given ranges; (ii) a disintegration time within the range 5-60 minutes; and (iii) a nicotine content from 0.5 to 10 mg or a corresponding content of said alkaloids.

This invention provides a method for making maytansinoids having a chiral amino acid side chain, such as DM1 and DM4 that are used to treat cancer. According to this method, the side chain may be added with little epimerization of the amino acid chiral center.

The invention discloses a method which can carry out qualitative screening to 242 compounds (drugs or toxicants) simultaneously. A mode of liquid chromatogram-mass spectrometry (LC-MS / MS) multi-reaction monitoring (MRM) is adopted to carry out determinedness to objects by two pairs of parent ion-daughter ion pair and retention time. The 242 drugs or toxicants comprise toxic products such as opioids, amphetamine and cocaines and the like, bromazepam such as benzodiazepines and barbiturates, and common drugs, alkaloid, pesticide (including organophosphates, carbamates, pyrethroid pesticide residues and organochlorine pesticide), weed killer, raticide and the like.

A new method and system for desorption ionization is described and applied to the ionization of various compounds, including peptides and proteins present on metal, polymer, and mineral surfaces. Desorption electrospray ionization (DESI) is carried out by directing charged droplets and / or ions of a liquid onto the surface to be analyzed. The impact of the charged particles on the surface produces gaseous ions of material originally present on the surface. The resulting mass spectra are similar to normal ESI mass spectra in that they show mainly singly or multiply charged molecular ions of the analytes. The DESI phenomenon was observed both in the case of conductive and insulator surfaces and for compounds ranging from nonpolar small molecules such as lycopene, the alkaloid coniceine, and small drugs through polar compounds such as peptides and proteins. Changes in the solution that is sprayed can be used to selectively ionize particular compounds, including those in biological matrices. In vivo analysis is demonstrated.

A method of treating and processing alkaloid, oil and protein-containing lupine seeds for the extraction of products from the lupine seeds by means of targeted fractionation is described, whereby the comminuted lupine seed is de-oiled by introducing a solvent and the residue is depleted of substances soluble in the acid range, preferably of alkaloids, by adding acids. The invention is characterized by the provisions that the lupine seeds are comminuted and / or shaped to form discoid flakes in such a way that after pre-crushing of the shelled or non-shelled seed containing the plant seeds the comminution of the plant seeds is carried out by means of a cooled flocculating roller, and the seed is heated by indirect supply of heat, largely with exclusion of water, and that after de-oiling the depletion of the flakes of substances soluble in the acid range, preferably of alkaloids, is performed by aqueous extraction, with a refined product of reduced alkaloid level and an aqueous extract being obtained.

The invention relates to devices and methods for delivering nicotine and / or other alkaloids from tobacco, other plants and other natural sources. More particularly, the invention relates to devices and methods for delivering an aerosol of nicotine to a user's lungs without combustion of the nicotine source materials.

A method for producing a consumable nicotine-alternative composition includes measuring a quantity of a cigarette nicotine-alternative alkaloid and / or a larger quantity of lobeline. The quantities of cigarette nicotine-alternative alkaloid and / or lobeline are diluted into one or more successive intermediate solutions, a last of which constitutes a final solution. The final solution is apportioned so that each portion contains a precise quantity of cigarette nicotine-alternative alkaloid and / or lobeline appropriate for consumption in a single use by a single person. Each portion is introduced into a separate single-serving dispenser.

A medicine with high activity to suppress alpha-glucosidase for treating diabetes, and hyperlipemia, and delaying sanility contains the common alkaloid extracted from mulberry plants and the flavone extract.

Four genes, A622, NBB1, PMT, and QPT, can be influenced for increasing nicotinic alkaloid levels in Nicotiana plants, as well as for synthesizing nicotinic alkaloids in non-nicotine producing plants and cells. In particular, overexpressing one or more of A622, NBB1, PMT, and QPT may be used to increase nicotine and nicotinic alkaloid levels in tobacco plants. Non-nicotine producing cells can be engineered to produce nicotine and related compounds by overexpressing A622 and

Materials derived from Citrus plants can be administered orally to humans for the purpose of producing or maintaining weight loss as well as for improving the person's physical performance and increasing the person's lean muscle mass. The Citrus materials include those portions of the plant that are normally considered waste or inedible, such as the leaves, peel, and immature, unripe fruit. The materials contain at least one of the alkaloids from the group consisting of synephrine, hordenine, octopamine, tyramine and N-methyltyramine (1). Two species, Citrus aurantium and Citrus reticulata, are particularly useful. The materials can be administered in their natural form or as extracts, and can be administered in various ways including capsules and tablets. The Citrus materials may also be used as a tea. For weight loss and weight control, the materials can be administered concurrently with caloric restriction or in the absence of caloric restriction. The materials may also be administered for the purpose of increasing muscle mass concurrently with a high protein diet as well as with an exercise program.

The invention discloses a hormone-free tissue culture method for dendrobium candidum, which comprises four tissue culture stages including germinating of seeds of the dendrobium candidum into spherical stems, multiplication and differentiation, and rootage and sound seedlings at stem sections; culture media used at the four stages include a hormone-free MS (Murashige and Skoog) culture medium, a hormone-free MS basal culture medium plus 15-20 g / l mashed potato culture medium, a hormone-free MS basal culture medium plus 15-20 g / l mashed banana plus 15-20 g / l mashed potato culture medium and a hormone-free 1 / 2 MS basal culture medium plus 15-20 g / l mashed banana plus 15-20 g / l mashed potato culture medium. The polysaccharide content and total alkaloid content in the culture of the dendrobium candidum obtained by hormone-free culture are increased compared with hormone-containing culture; the growth state of the dendrobium candidum is normal; the genetic diversity of the plant variety is protected; with the adoption of the method, the tissue culture of the dendrobium candidum is more convenient, green and environmentally friendly, and the resources are saved.

The invented Pu Er tea extract is dark reddish brown colour, can be dissolved in water and does not dissolve in ethyl alcohol, methyl alcohol and trichloromethane, its main chemical components are ; 15%-45% of tea-polysaccharide, 50%-70% of tea alkaloids, 4.7%-14.0% of protein and the rest is minute quantity of tea self-body substances, such as thearubigens, theaflavin and water soluble pectin. It can be used as raw material for preparing oral liquor, soft capsule, capsule and lozenge, etc.

The invention relates to a complex extraction process for mulberry leaf flavonoid, polysaccharide and alkaloid through cogeneration, which is characterized by having the following extraction steps: raw material processing, extraction, and separation. The complex extraction process adopting a complex extraction method for the mulberry leaf flavonoid, the polysaccharide and the alkaloid obtained through the cogeneration has the advantages of mild condition, low energy consumption, small environment pollution; macroporous absorbent resin and chloroform used in the complex extraction process can be reused through regeneration; the economic value of the obtained effective ingredients is very high; the mulberry leaf resource is greatly saved; a new utilization approach of the agricultural product mulberry leaf is opened up; and the medicinal utilization value and the economic added value of the mulberry leaf are improved.

A method for the administration of nicotine, THC, tobacco, cannabidiol or a base alkaloid includes administering an absorption conditioning unit having at least two agents selected from the group consisting of (a) a buffer agent, (b) a capturing agent, (c) a penetration agent, and (d) a thermal agent, to the mammal, and then administering by inhalation a bioactive agent selected from the group consisting of nicotine, THC, cannabidiol and a base alkaloid. The absorption conditioning unit may be in a dosage form not containing a drug. The absorption conditioning unit may create a pH in the oral cavity of 7.8-10 for a period of ten minutes or more after administration, the dosage form not containing an acid and not containing a drug.

The invention relates to a method for preparing flavors and fragrances, in particular to a method for catalyzing and adjusting Maillard reaction to produce tobacco flavor by adopting natural organic acid or alkaloid. When the tobacco flavor is prepared by using the Maillard reaction, before the reaction is carried out, pH value of a reaction system is adjusted to be 2-11 by a natural plant extractive containing organic acid or alkaloid, and then the Maillard reaction is catalyzed. In the invention, the source of raw materials is wide, the obtained flavor has high fragrance intensity and rich quality of aroma, thereby the quality of tobacco is improved greatly.

The invention discloses a method for extracting various active ingredients from dendrobium officinale. The method comprises the following steps of: carrying out flash-extracting; centrifuging the flash-extracted crude extracting solution; carrying out alcohol precipitation and tertiary dehydration to the centrifuged liquid supernatant to obtain free polysaccharide; combining the centrifuged precipitates with polysaccharide and alkaloid extract; and extracting the various active ingredients from the dendrobium officinale, so that the comprehensive utilization of the dendrobium officinale resources is realized. The method disclosed by the invention is high in yield, capable of greatly shortening the extraction time during the extraction process, capable of avoiding the influences of thermal destruction to the products, capable of reducing the production cost and applicable to large-scale production.

The invention provides an aquatic animal preparation capable of protecting liver. The aquatic animal preparation capable of protecting liver is composed of an A preparation and a B preparation. The A preparation is composed of 70 to 100% of a Chinese herbal medicine mixture, 0 to 15% of a flavonoid compound, 0 to 15% of a saponin compound, 0 to 15% of an alkaloid, 0 to 15% of trace element, and 0 to 15% of a polysaccharide; and the B preparation is one or a mixture of a plurality of ingredients selected from a feed additive, a photosynthesis accelerant, and a vitamin. In vivo, the aquatic animal preparation is capable of increasing the activity of liver cells, repairing cell damage, removing a plurality of toxins in aquatic animals, relieving liver burden, improving immunity, resisting free radical injury, reducing lipid peroxide, protecting liver, nourishing gallbladder, and preventing tumor; beneficial substances of living bacteria agents and Chinese herbal particles dissolved in water are capable of inhibiting planting of pathogenic bacteria in water bodies, and killing virokine.

This invention provides a method for making maytansinoids having a chiral amino acid side chain, such as DM1 and DM4 that are used to treat cancer. According to this method, the side chain may be added with little epimerization of the amino acid chiral center.

The present invention is directed to novel compositions of bio-active compounds comprising 4-hydroxyisoleucine and one or more compounds selected from the group of amino acids, alkaloids, glycosides, volatile oils, saponins, sapogenins, mannans, flavonoids, fatty acids, vitamins and provitamins, minerals, and carbohydrates. Preferably, the novel compositions of bio-active compounds include 4-hydroxyisoleucine and one or more amino acids selected from the group consisting of arginine, aspartate, threonine, serine, glutamate, proline, glycine, alanine, cysteine, valine, methionine, isoleucine, leucine, tryptophan, phenylalanine, ornithine, proline, lysine, histidine, and gamma-aminobutyrate. The composition of bio-active compounds preferably include between about ten percent and about seventy percent of 4-hydroxyisoleucine and between about twenty percent and about forty percent of other amino acids. The bio-active compounds of the novel composition of the present invention may be derived, isolated, and / or extracted from Fenugreek seeds. A preferred method for extracting the bio-active compounds from Fenugreek seeds includes the steps of: (1) providing a plurality of Fenugreek seeds; (2) preparing the Fenugreek seeds; and (3) extracting a novel composition of bio-active compounds from the Fenugreek seeds, which include a preliminary extraction step and a secondary extraction step. The compositions of bio-active compounds have been found to be helpful in restoring healthy energy balance in humans and animals, aiding in weight management efforts, and for balancing blood sugar levels by way of assisting the body to make more efficient use of existing (i.e., endogenous) insulin.

A food process comprises starting with hemp (Cannabis sativa) seeds that have been cultivated to have low levels of tetrahydrocannabinol (THC) alkaloid, e.g., less than 0.3%. The hemp seeds are dehulled to produce split seed kernels, mixing the split seed kernels with hot water to hydrate them into a slurry, grinding the slurry to blend and smooth it into a product base, cooking the product base to achieve a particular flavor and aroma consistent with a target food product, cooling the product base to stop cooking, and further processing the product base into a target food product like soups and beverages. The products produced have high levels of protein, vitamins, and other nutritional values.

The invention discloses a method for preparing antibiotic substituent for feed by probiotic asynchronous fermentation of Chinese herbal medicine, belonging to the field of food nutrition and the technical fields of microorganisms and feed. The method comprises the following steps: extracting Chinese herbal medicine by adopting asynchronous fermentation and enzymolysis technologies, namely adding water and nutritive salt in proportion to prepare a Chinese herbal medicine fermentation liquid after a plurality of Chinese herbal medicines are subjected to impurity removal and super-micro smashing; firstly inoculating Aspergillus niger for aerobic fermentation, thus cellulose and pectinase are prepared; and then inoculating enterococcus faecium and lactobacillus plantarum for anaerobic fermentation; and then carrying out enzymolysis on damage cell walls, and dissolving out active substances of saponin, alkaloid, phytosterol, enzymic preparations, organic acid and the like. A plate-and-frame filter press is adopted to filter the fermentation liquid, the filtration liquid is directly subjected to low-temperature spray drying to prepare antibiotic substituent powder A; a filtration cake is dried by adopting a roller drier to prepare antibiotic substituent powder B, and the power A and the powder B are mixed and formulated in proportion to replace fermented enzymolysis Chinese herbal medicine extracts of antibiotic for feed.

The invention discloses shell powder biological environment-friendly internal wall coating, which comprises the following components in part by weight: 240 to 350 parts of water, 1 to 3 parts of alkaloid, 2 to 8 parts of dispersant, 1 to 3 parts of wetting agent, 2 to 6 parts of defoaming agent, 100 to 200 parts of shell powder, 150 to 250 parts of titanium white, 30 to 80 parts of talcpowder, 150 to 300 parts of acrylic emulsion, 25 to 75 parts of gelatin, 10 to 30 parts of glycerol, 1 to 3 parts of ethanol and 1 to 2 parts of preservative. The paint film formed by the coating disclosed by the invention is full, and the coating disclosed by the invention has good decorative and protective functions and very high environment friendliness, is free from materials harmful to the environment and human bodies, avoids polluting the environment and is a shell powder interior wall coating with high comprehensive performance.