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Method for preparing antibiotic substituent for feed by probiotic asynchronous fermentation of Chinese herbal medicine

An asynchronous fermentation, Chinese herbal medicine technology, applied in the fields of microorganisms, feed, and food nutrition, can solve the problems such as the lack of separate drying of medicinal residues and medicinal liquids, the lack of scientific prescriptions, and the singleness

Active Publication Date: 2012-04-11
河南安信生物科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is: it is intended to overcome the research or products of the few Chinese herbal medicines extracted by fermentation for feeding on the above-mentioned market that are all single aerobic fermentation or anaerobic fermentation, and the fermentation products or researches of Chinese herbal medicines are also mostly single However, due to the lack of scientific formulas and the lack of separate drying of medicinal residues and medicinal liquids, it is planned to use asynchronous fermentation and enzymatic hydrolysis technology to extract Chinese herbal medicines, that is, to remove impurities and ultrafinely pulverize several kinds of Chinese herbal medicines for feeding, and then add water and nutrient salts in proportion to make them. To form a Chinese herbal medicine fermentation liquid, first insert Aspergillus niger for aerobic fermentation to produce cellulase and pectinase, then insert Enterococcus lactis and Lactobacillus plantarum for anaerobic fermentation, and then enzymatically hydrolyze the damaged cell wall to dissolve saponins and alkaloids , sterol organic acids and other active substances

Method used

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  • Method for preparing antibiotic substituent for feed by probiotic asynchronous fermentation of Chinese herbal medicine
  • Method for preparing antibiotic substituent for feed by probiotic asynchronous fermentation of Chinese herbal medicine
  • Method for preparing antibiotic substituent for feed by probiotic asynchronous fermentation of Chinese herbal medicine

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Weigh 1000kg of camellia seed cake powder, 300kg of eucommia leaves, 300kg of astragalus, 250kg of hawthorn, 250kg of pine needle powder, 200kg of epimedium, and 200kg of licorice. 120 meshes (crossing a 0.15mm sieve) are made into compound Chinese herbal medicine for subsequent use.

[0024] Weigh 100kg of glucose, 24kg of yeast extract, K 2 HPO 4 15kg, NaCl 6kg, MgSO 4 0.9kg, mixed to make nutrient salt for later use.

[0025] After activating the strains of Aspergillus niger, Enterococcus lactis and Lactobacillus plantarum twice with slant and MRS liquid medium, they were inoculated into 250mL Erlenmeyer flasks with a liquid volume of 100mL in each bottle. 7 The CFU / mL concentration was added to the 5L liquid seed fermenter, and then press 1.5×10 7 The CFU / mL concentration was expanded into a 50L liquid seed fermenter, cultured and fermented at 39°C for 24 hours, and the Aspergillus niger strain liquid and the mixed strain liquid of Enterococcus lactis and Lactob...

Embodiment 2

[0031] Weigh 500kg of camellia seed cake powder, 150kg of eucommia leaves, 150kg of astragalus, 125kg of hawthorn, 125kg of pine needle powder, 100kg of epimedium, and 100kg of licorice. 120 meshes (crossing a 0.15mm sieve) are made into compound Chinese herbal medicine for subsequent use.

[0032] Weigh 50kg of glucose, 12kg of yeast extract, K 2 HPO 4 7.5kg, NaCl 3kg, MgSO 4 0.45kg, mixed to make nutrient salt for later use.

[0033] After activating the strains of Aspergillus niger, Enterococcus lactis and Lactobacillus plantarum twice with slant and MRS liquid medium, they were inoculated into 250mL Erlenmeyer flasks with a liquid volume of 100mL in each bottle. 7 The CFU / mL concentration was added to the 5L liquid seed fermenter, and then press 1.5×10 7 The CFU / mL concentration was expanded into a 50L liquid seed fermenter, cultured and fermented at 39°C for 24 hours, and the Aspergillus niger strain liquid and the mixed strain liquid of Enterococcus lactis and Lactob...

Embodiment 3

[0039] Weigh 5000kg of camellia seed cake powder, 1500kg of eucommia leaves, 1500kg of astragalus, 1250kg of hawthorn, 1250kg of pine needle powder, 1000kg of epimedium, and 1000kg of licorice. 120 meshes (crossing a 0.15mm sieve) are made into compound Chinese herbal medicine for subsequent use.

[0040] Weigh 200kg of glucose, 48kg of yeast extract, K 2 HPO 4 30kg, NaCl 12kg, MgSO 4 1.8kg, mixed to make nutrient salt for later use.

[0041] After activating the strains of Aspergillus niger, Enterococcus lactis and Lactobacillus plantarum twice with slant and MRS liquid medium, they were inoculated into 250mL Erlenmeyer flasks with a liquid volume of 100mL in each bottle. 7 The CFU / mL concentration was added to the 5L liquid seed fermenter, and then press 1.5×10 7 The CFU / mL concentration was expanded into a 50L liquid seed fermenter, cultured and fermented at 39°C for 24 hours, and the Aspergillus niger strain liquid and the mixed strain liquid of Enterococcus lactis and...

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Abstract

The invention discloses a method for preparing antibiotic substituent for feed by probiotic asynchronous fermentation of Chinese herbal medicine, belonging to the field of food nutrition and the technical fields of microorganisms and feed. The method comprises the following steps: extracting Chinese herbal medicine by adopting asynchronous fermentation and enzymolysis technologies, namely adding water and nutritive salt in proportion to prepare a Chinese herbal medicine fermentation liquid after a plurality of Chinese herbal medicines are subjected to impurity removal and super-micro smashing; firstly inoculating Aspergillus niger for aerobic fermentation, thus cellulose and pectinase are prepared; and then inoculating enterococcus faecium and lactobacillus plantarum for anaerobic fermentation; and then carrying out enzymolysis on damage cell walls, and dissolving out active substances of saponin, alkaloid, phytosterol, enzymic preparations, organic acid and the like. A plate-and-frame filter press is adopted to filter the fermentation liquid, the filtration liquid is directly subjected to low-temperature spray drying to prepare antibiotic substituent powder A; a filtration cake is dried by adopting a roller drier to prepare antibiotic substituent powder B, and the power A and the powder B are mixed and formulated in proportion to replace fermented enzymolysis Chinese herbal medicine extracts of antibiotic for feed.

Description

technical field [0001] The invention belongs to the technical field of food nutrition, microbes and feed, and specifically relates to the asynchronous fermentation of Aspergillus niger and lactic acid bacteria to prepare Chinese herbal medicines such as camellia seed cake (meal) powder, ulmoides ulmoides leaves, astragalus, hawthorn, pine needle powder, epimedium and licorice, which can replace feed Fermentation and enzymatic hydrolysis of antibiotic herbal extracts. Background technique [0002] In recent years, food safety incidents have occurred frequently, and the hazards of antibiotics have attracted much attention: first, antibiotics are likely to remain in livestock and poultry products such as meat, eggs, and milk, endangering human health; Increased drug resistance. Therefore, some countries have banned its use. In 1974, the European Community prohibited the use of penicillin and tetracycline in feed, and in 1998, the use of spiramycin, bacitracin zinc, virginiamy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/17A23K20/195
Inventor 程茂基孟秀丽吴成章薛芹
Owner 河南安信生物科技股份有限公司
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