42results about How to "Improve metabolic energy" patented technology

The invention discloses a complex enzyme preparation for feeding piglets. The complex enzyme preparation for feeding piglets comprises the following seven enzymes: acid protease, amylase, xylanase, beta-glucanase, cellulase, pectinase and phytase; and the enzymatic activity ratio of the seven enzymes is 1: 1: (8-10): (3.3-4): 1: (0.5-0.55): (0-0.02). By applying the complex enzyme preparation disclosed by the invention, various anti-nutritional factors in feed can be degraded, the viscosity of chyme in an intestinal tract can be reduced, and the metabolic energy of the feed can be improved; the protein and starch digestibility of the piglets can be improved; excessive reproduction of harmful microorganisms in the intestinal tract can be reduced, damage to the intestinal wall can be reduced, and the microecological balance of the intestinal tract can be regulated; the survival rate and disease resistance of the piglets can be improved, the diarrhea rate can be reduced, and the overall uniformity is improved; and the weight gaining of the piglets can be promoted, and the cultivation cost is reduced.

The invention discloses a complex enzyme preparation for a growing and fattening pig feed. The complex enzyme preparation for the growing and fattening pig feed comprises the following eight enzymes: acid protease, amylase, beta-mannase, xylanase, beta-dextranase, cellulase, pectinase and phytase; and the enzyme activity ratio of the eight enzymes is sequentially 1: (0.8-14.5): (0.19-0.3): (33.7-35): (25-30): (3.2-3.5): (0.19-2.0): (0-0.025). The complex enzyme preparation can degrade various anti-nutritional factors in the feed, reduce the chime viscosity of the intestinal tract, improve the metabolic energy of the feed, improve the digestion rate of growing and fattening pigs on protein and starch, reduce the nutrient content of the chime entering the intestinal tract, effectively inhibit the reproduction of harmful microbes in the intestinal tract, reconcile the micro-ecological balance of the intestinal tract, promote the health of the pigs, improve the average daily gain of the growing and fattening pigs, reduce the feed-meat ratio, shorten the maintenance period and improve the economic benefit.

The invention discloses lactation compound feed for sows and a preparation method thereof. The lactation compound feed for sows is made from wheat, corn in northeast China, 46% soybean meal, soybean hull, calcium hydrophosphate, rice bran oil, liquid phytase, liquid compound enzyme, limestone flour, salt, 65% lysine, threonine, 60% choline chloride, antioxidant, plasma protein, potassium chloride, magnesium sulfate, vitamin prefix, and trace mineral premix. The preparation method include: weighing all materials by percent weight, and adding the materials into a crusher for crushing under the crushed size of 25-40 mesh; and adding the crushed materials into a pelletizer to make pellets at 85 DEG C with gelatinization degree reaching more than 90%. The lactation compound feed for sows has the advantages that the lactation compound feed for sows meets requirements of nursing sows for nutrition and is high in palatability, immunity, milk yield and milk quality are increased for the sows, and productivity of piglets is further improved.

The invention discloses feed for weaned piglets. The feed comprises the following raw materials in parts by weight: 45-55 parts of corn, 10-20 parts of wheat, 5-10 parts of extruded soybean, 10-20 parts of bean pulp, 3-7 parts of wheat middling, 1-4 parts of soybean oil, 1-4 parts of steamed fish powder, 2-5 parts of glucose, 0.2-0.4 part of acidifying agent, 1.2-1.5 parts of calcium hydrophosphate, 0.7-1.0 part of stone powder, 0.1-0.3 part of salt, 0.3-0.5 part of compound amino acid, 0.1-0.3 part of compound vitamin, 0.1-0.3 part of compound trace element, 0.05-0.15 part of enzyme preparation and 0.05-0.15 part of microecological preparation. The feed is used for the weaned piglets with the age of 6-10 weeks, and has the beneficial effects that the feed intake of the weaned piglets can be increased, the growth can be enhanced, the feed reward can be improved, the diarrhea rate and the feed-changing stress of the weaned piglets can be reduced and the use of antibiotics and bad results caused by the antibiotics are reduced.

The invention discloses broiler chicken preliminary compound feed and a preparation method thereof. The compound feed comprises the following raw materials: broiler compound premix, salt, threonine, Maxus premix, stone dust, calcium hydrophosphate, lysine of 65 percent, liquid phytase, poultry mineral premix, solid methionine, rape-seed cake, rice bran oil, corn protein powder of 60 percent, bean dreg of 46 percent, choline chloride, corn, wheat and liquid wheat enzyme. The preparation method comprises the following steps that: raw materials are weighed according to the weight percentage to be put into a pulverizer; the pulverized mixture is granulated under the temperature of 75 DEG C to 80 DEG C, and a degree of gelatinization reaches more than 90 precent; and the produced particles are put into a crusher to be broken, and the breaking granularity is 8 meshes to 15 meshes. The broiler chicken preliminary compound feed has beneficial effects that: the preliminary nutrition demand of broiler chicken can be satisfied, antinutritional factors in wheat daily ration can be adequately decomposed, nutrient absorption can be increased, the conversion rate of the feed can be improved, the survival rate of pups and young poultry can be improved, and the production performance of the broiler chicken can be improved.

The invention relates to the genetic engineering field, especially to a strain Bispora sp.MEY-1 which produces an acidic xylanase and the acidic xylanase XYL10A got from the strain having an amino acid sequence shown as SEQ ID NO.1 or 2, a gene for coding said xylanase having a nucleotide sequence shown as SEQ ID NO.1 or 2, and application of a recombinant vector, a recombinant strain and a recombinant enzyme containing said gene. The xylanase of the invention has the following advantages: the optimum pH 2.4, the optimum temperature 80 DEG C, good pH stability and thermostability, specific activity 5437 U / mg, good protease resistance and easy for industrial fermentation production. The product of the invention can be widely used for the animal feeding-stuffs, the food, the medicament, the brewing and the energy industry as a novel enzyme preparation.

The invention relates to a drinking type acidulant and a preparation method and application thereof. Each 100ml of the acidulant contains components of 15-25ml of formic acid, 25-35ml of lactic acid,15-25g of citric acid, 5-15g of fumaric acid, 1-8g of calcium formate, 1-8g of sodium butyrate and the balance deionized water, wherein all of the components are 100ml. The preparation method comprises the following steps of performing injection according to the amount of 50-80% of the total amount of water to a stirring tank, starting a power supply, performing stirring at 120r / min, slowly addingthe fumaric acid, then adding the calcium formate and the sodium butyrate, performing stirring for 15min, after the fumaric acid, the calcium formate and the sodium butyrate sufficiently dissolve inthe water, slowly adding the citric acid, the lactic acid and the formic acid separately, and finally, adding the remaining water for uniform stirring so as to obtain products. Compared with the priorart, the drinking type acidulant contains various organic acid and organic acid salts, can regulate the acid of a ration system, can reduce the pH value of the digestive tracts of animals, can improve the activity of digestive enzymes, can promote the digestion of nutrient substances, can effectively control diarrhoea of piggies at the weaning and child care period, can regulate the intestinal microecology balanced, and can promote growth of the piggies at the weaning and child care period. Besides, when the drinking type acidulant is drunk, growth and propagation of pathogenic microorganismsin animal organisms, feeds and environment can be greatly reduced, and the usage amount of antibiotics can be directly reduced, so that drug tolerance, remaining and pollution problems caused by antibiotics can be reduced.

The invention discloses a middle broiler feed. The middle broiler feed comprises the following raw materials in parts by weight: 60-80 parts of corns, 5-30 parts of soybean meal, 1-10 parts of corn gluten meal, 0-5 parts of fish meal, 0.5-3 parts of soybean oil, 0.8-2.5 parts of fine stone powder, 0.8-2.5 parts of calcium hydrophosphate, 0.1-1.2 parts of amino acid, 0.1-0.8 part of vitamin premix and 0.3-1.2 parts of microelement premix. The amino acid level of the middle broiler feed adapts to that of an animal according to an ideal amino acid mode; the digestibility is improved, and particularly, the utilization rate of feed proteins and the utilization rate of amino acid are improved; generation of ammonia gas in a henhouse is reduced; synthetic amino acid is appropriately added, and the crude protein level is correspondingly reduced, so that protein feed resources can be saved, and the output of nitrogen in the excreta of livestock and poultry can be reduced; the daily gain is high, the feed conversion ratio is low, the raising cycle is greatly shortened, the slaughtering rate is improved, and economic benefits are remarkable.

The invention relates to the field of genetic engineering, in particular to an acidophil Beta-glucanase GLU7A and gene and application thereof. The invention provides a glucanase GLU7A from acidophil bispora Bisporasp, and the amino acid sequence thereof is shown as SEQIDNO.1, and the invention provides a genome of coding the glucanase and cDNA coding gene glu7A. The invention obtains an acidophil glucanase which has the optimum pH value of 1.5-5.0, simultaneously remains high enzymatic activity in acid environment, and resists protease hydrolysis. In addition, the glucanase not only hydrolyzes Beta-1, 3-1, 4-glucanase, but also can hydrolyze cellulose. Due to the properties, the acidophil Beta-glucanase GLU7A can be applied in the industries of food, feedstuff and beer.

The invention relates to the field of gene engineering, in particular to acid xylanase XYNB, genes and application thereof. A strain Bispora sp. X-1 of acid producing xylanase is separated, the acid xylanase which has an amino acid sequence expressed as SEQ ID NO.1 or 2 is obtained from the strain, the genes for coding the xylanase is obtained, and the genes have a nucleotide sequence expressed as SEQ ID NO.3 or 4. The xylanase of the invention has the following properties: at the optimal pH of 2.6 and the optimal temperature of 65 DEG C, the xylanase has good pH stability and thermal stability; the specific activity is 2,049 U / mg; and the xylanase has extremely good proteinase resistance and is suitable for industrialized fermentation production. The xylanase serving as a novel enzyme preparation can be widely used in the industries of animal feed, food, medicine, wine brewing, energy and the like.

The invention belongs to the technical field of feed additives, and particularly relates to a feed additive for improving immunity of cows in the milk production period by use of perilla and a preparation method of the feed additive. The feed additive comprises perilla antibacterial peptide, fermented alfalfa meal, a traditional Chinese medicine extract preparation and a complex enzyme preparation. The perilla antibacterial peptide is prepared from perilla seed meal left after oil pressing of perilla seeds. The perilla antibacterial peptide acts together with perilla leaf, dandelion, honeysuckle flower, Shuanghuanglian, fructus forsythiae and isatis root extracts with antibacterial and anti-inflammatory effects in the feed additive and can effectively treat and prevent mastitis of the cowsand improve the immunity of the cows in the milk production period. The fermented alfalfa meal, the traditional Chinese medicine extract preparation and the complex enzyme preparation in the feed additive provide a richer and more balanced nutrient basis for the cows and can effectively promote milk secretion and improve the yield and quality of milk, and green milk without hormones, antibioticsand chemical residues can be obtained.

The invention provides a neutral high temperature xylanase CtXyn10A as well as genes and application thereof. The neutral high temperature xylanase CtXyn10A has proteins of amino acid sequences shown in SEQ ID No. 3 and / or SEQ ID No. 5 in a sequence table; and / or is characterized in that amino acid residue sequences shown in SEQ ID No. 3 and / or SEQ ID No. 5 in the sequence table are replaced and / or deleted and / or added by one or several amino acid residues, and have proteins with xylanase activity and derived from the proteins with the amino acid sequences shown in SEQ ID No. 3 and / or SEQ ID No. 5 in the sequence table. The xylanase CtXyn10A has an optimum pH of 6.0 to 7.0 and an optimum temperature of 70 DEG C, keeps enzyme activity of 20% or above at 90 DEG C and pH 9.0, and specific activity is 461 U / mg; the xylanase CtXyn10A has the activity of xylanase, glucanase and cellulase, is easy to produce by industrial fermentation, and can be widely used in food, paper, energy industry and the like as a novel broad-spectrum enzyme preparation.

The invention relates to the field of genetic engineering, in particular to high-specific-activity xylanase XYN11F63 and genes and application thereof. The invention provides xylanase XYN11F63 derived from Penicillium sp.F63 (CGMCC No.1669), wherein the amino acid sequences thereof is shown in SEQ ID No.1; and the invention further provides the genome for coding the xylanase and cDNA-coding genes xyn11F63. The xylanase of the invention has the following properties: the optimal pH value thereof is 4.5, the optimal temperature thereof is 40 DEG C, and the specific activity thereof is 7,988U / mg, and the xylanase has good proteinase resistance and is suitable for industrialized fermentation production; and the xylanase is widely applicable as a novel enzymic preparation in the industries of animal feed, food, papermaking, energy and the like.

A sports drink is presented, along with its method of composition and use. The sports drink of the present invention is produced primarily with natural and organic ingredients, yet provides a good-tasting beverage that ameliorates the effects of strenuous exercise and assists with obtaining a desirable body mass index.

The present invention relates to codon-optimized xylanase coding sequences and the expression of xylanases in microbes and yeast. The invention further relates to using multiple copies of the xylanase expression construct for high levels of protein expression. The invention also relates to the use of xylanases as feed or food additives. The invention also relates to methods of expression of enzymes to increase thermotolerance by expressing them in organisms that glycosylate proteins compared to expression that the same enzyme without the glycosylation. Further, the invention relates to methods of preparing feed, enzyme feed additives, and methods of reducing the feed conversion ration or increasing weight gain of animals.

The invention provides a medicinal paste for treating Chinese honeylocust spine and hyperosteogeny and its preparing process, wherein the raw material herbs include the following constituents (by weight ratio): Chinese angelica root, Chinese ephedra, rhubarb horsetails, radix paeoniae rubrathe, dried rehmannia root, goldthread root, licorice root, thunberg fritillary bulb, honeysuckle flower, safflower, stem pith of the ricepapr plant, dahurian angelica root, radix aconiti. and the preparing process steps of cooling down the plaster and charging the following fine materials: pangolin scales, camphor, musk, boneol, frankincense, myrrh, and dragon's blood resin.

The present invention provides novel soybean lines having high sucrose content and low phytic acid and low stachyose content. The soybeans are easily digested and provide high energy content for animals and humans. The low phytic acid content permits animal feed to be produced that does not require phytase, yet does not result in significant production of pollution to the environment from excretion by farm animals.

The invention discloses a complex enzyme preparation for a feed of a mink in a winter fur growth period. The complex enzyme preparation for the feed of the mink in the winter fur growth period comprises the following six enzymes: amylase, acid proteinase, araboxylan enzyme, Beta-mannase, pectinase and phytase. The complex enzyme preparation can effectively fill the gap on endogenous enzyme of the mink, degrades the main antinutritional factors, namely, non-starch polysaccharides (NSP), in a vegetable feedstuff, reduces the gut digesta viscosity of the alimentary canal of the mink, and improves the digestibility utilization rate of the mink to the maize-bean pulp-fish meal type daily ration. By adding the complex enzyme preparation, the apparent metabolizable energy (AME) in the daily ration can be increased, the digestibility and utilization rate of the protein and starch can be raised, the excessive multiplication of spoilage organisms in the intestinal canal can be reduced, the microecological balance of the intestinal canal can be adjusted, the diarrhea rate is reduced, the premunition and the survival rate of the mink in the winter fur growth period can be increased, the feed conversion rate is raised, the average daily weight gain of the mink is increased, and the dimension of the skin of the mink is expanded, the costs of the feed and breeding are decreased, and the economic benefit is increased.

The invention discloses a pig feed formula and relates to the technical field of raising pig. The pig feed formula comprises, by quality, wheat from 30% to 70%, corn from 0% to 50%, pine needle powder from 5% to 10%, premix compound from 5% to 10% and concentrated feed special for the wheat from 5% to 10%. The pig feed formula is characterized in that the concentrated feed special for the wheat comprises, by quality, bean pulp from 60% to 70% and enzyme special for the wheat from 30% to 40%. The enzyme special for the wheat comprises beta-dextranase, pectinase, amylase and protease. The pig feed formula has the advantages of improving flexibility of the formula, improving metabolic energy of the wheat, improving crude protein apparent digestibility of the wheat due to the fact that the wheat can partly or overall replace the corn in formula feed, narrowing the gap between the corn and the wheat, reducing cost of feed on the premise of maintaining production performance of animals, relieving viscosity effect of the wheat, adjusting intestinal tract micro-ecological faunal, improving intestinal health, keeping excrement dry, reducing excrement excretion quantity and pollution of environment and product.

The present invention discloses a selenium enriched feed of young broiler chickens in late fattening period and a preparation method thereof. The selenium enriched feed comprises the following components: corn, wheat bran, barley germs, sweet potato buds and seedlings, lotus root starch, medical stones, stir-fried soybean flour, watermelon seeds, setaria viridis seeds, duck eggshell powder, edible salt, allicin, and sesame seed oil. Compared with the prior art, the feed has the following advantages: by a reasonable proportion of the raw materials, metabolizable energy in the feed is appropriately increased, protein contents are reduced, nutrition is balanced, feed cost is reduced, the weight gains of the young broiler chickens are ensured, and the improvements of the contents of selenium and other nutrients in the feed by adding expensive selenium-enriched coarse cereals are avoided. The sweet potato stem segments or top end tender buds are used to conduct biotransformation of inorganic selenium in the medical stone and sodium selenite solution, so that the available selenium content in the feed is improved, the selenium content in the chicken meat is about 0.4 mg / kg, the young chickens are better in tastes, and the feed has efficacies of enhancing strength and preventing diseases in a long-term consumption.

The invention discloses a method used for preparing a pig feed by taking sweet potato vine as a raw material. The pig feed is prepared from, by weight, 70 to 80 parts of sweet potato vine, 20 to 30 parts of corn, 0.1 to 0.3 part of multi vitamin, 0.05 to 0.15 part of a biological fattening agent, 0.5 to 1.5 parts of bicarbonate soda, 0.008 to 0.012 part of saccharin, 0.008 to 0.012 part of a fermentation mother liquid; the fermentation mother liquid is prepared from soybean whey, lactobacillus acidophilus, and bacillus subtilis at a volume ratio of (90-100):(2-4):1; water content is controlled to be 60 to 70wt%; temperature is controlled to be higher than 0 DEG C; and the pig feed prepared from sweet potato vine is obtained via 60 to 84h of sealed fermentation. Before degradation, only 12% of the nutrients of sweet potato vine can be absorbed by animals, and after degradation, 93% of the nutrients can be absorbed; protein digestion rate after degradation is seven times higher than that before degradation; crude protein content is increased by more than 50%; digestible energy and metabolizable energy are increased greatly; and vitamin content is increased by 3 to 30 times.

The invention discloses a complex enzyme feed additive and application thereof. The complex enzyme feed additive is prepared from xylanase, beta-glucan, beta-mannanase, cellulase, protease, amylase and lipase, and the additive is added into broiler chicken feed processed through new corn with the adding amount of 200 g / ton. The complex enzyme feed additive has the advantages that the broiler chicken feed processed through new corn is added with the complex enzyme feed additive, non-starch polysaccharides in the feed can be effectively hydrolyzed, the metabolic energy of the feed and the feed conversion rate are increased, and the feed conversion ratio is lowered, so that the feed cost is lowered, and significant economic benefits are achieved.

The invention relates to a compound enzyme preparation capable of improving production properties and intestinal microecology environment of broiler chickens, and belongs to the technical field of feed additives. The compound enzyme preparation capable of improving production properties and intestinal microecology environment of broiler chickens comprises the following components by content: 100-250U / g of alpha-galactase, 2000-4000U / g of beta-mannan, 2000-3000U / g of beta-glucanase, 500-3000U / g of amylase, 500-1000U / g of cellulase, 15000-25000U / g of xylanase and 1000-1500U / g of pectinase, wherein the content of all the components is measured by enzyme activity. The compound enzyme preparation disclosed by the invention is simple in formula; and after the compound enzyme preparation is addedto a feed, the production properties of livestock and poultry are improved, the utilization rate of raw materials of the feed is increased, and feed resources are saved.

The invention discloses nutritional feed suitable for piglets and a preparation method of the nutritional feed. The nutritional feed suitable for the piglets is prepared from the following raw materials : hulled corns, puffed wheat, a granular premix, puffed soybean meal, fermented soybean meal, steamed fish meal, whey powder, sucrose, glucose, monocalcium phosphate, table salt, choline chloride,zinc oxide, lysine, threonine, methionine, tryptophan, a compound acidifier, a vitamin premix, an antioxidant, beta-mannase and lactic acid bacteria. The nutritional feed is prepared through the following steps: S1, preparing basic powder; S2, preparing a powdery premix; and S3, stirring and mixing the powdery premix with the granular premix, beta-mannase and lactic acid bacteria at the room temperature so as to obtain the nutritional feed suitable for the piglets. Through the nutritional feed, the problem existing in the prior art that conventional feed is not suitable for the imperfect physique of the digestive system, the micro-ecological system and the immune system of the piglets is solved.

The invention discloses an energy cake for pets. The energy cake comprises a component A, a component B and a component C, wherein the component A comprises the following components in parts by weight: 8-12 parts of yoghourt, 1-3 parts of vegetable fat powder, 0.5-1 part of distilled glyceryl monostearate, 1-2 parts of vegetable oil and 7-10 parts of water; the component B comprises the following components in parts by weight: 15-18 parts of cereal flour, 8-10 parts of vegetable protein, 8-12 parts of white granulated sugar and 1-2 parts of table salt; the component C comprises the following component in parts by weight: 40-50 parts of cereals. The energy cake is simple in formulation, rich in nutrition and novel in shape, and can quickly supply energy; the raw materials are easy to obtain. The invention further discloses a preparation method of the energy cake for pets. The preparation method comprises few steps, and is simple in process and safe in operation.

The invention discloses a feed formula suitable for flying of adult swan gooses, which comprises 30% of granules, 10% of rice, 10% of sorghum, 10% of crushed corn, 5% of fructus cannabis, 5% of soybean meal, 5% of bran and 25% of cooked corn. The daily consumption of the swan gooses flying every day can be satisfied by the feed formula, and the flying ability, metabolic ability and resistance ability of the swan gooses can be guaranteed.

The invention relates to the field of genetic engineering, in particular to neutral cold-adaptive xylanase CaXyn11A as well as a gene and an application thereof. The amino acid sequence of the neutral cold-adaptive xylanase CaXyn11A is as shown in SEQ ID NO.1; the xylanase disclosed by the invention has the following properties: optimum pH value is 6.5, the optimum temperature is 40 DEG C, an enzyme activity is about 50% at 20 DEG C and a specific activity is 357.3U / mg, a catalysis efficiency at ambient temperature (20 DEG C) is higher than the optimum temperature (40 DEG C), and the xylanase is easy for industrial fermentation production. The xylanase, as a novel enzyme preparation, can be widely applied to aquatic feed, food, paper making, energy industry and the like.

The invention discloses a complex enzyme preparation special for wheat-based diets. The complex enzyme preparation is characterized by being prepared from a carrier, xylanase, cellulase, amylase, alpha-galactosidase and beta-mannase. In each gram of the complex enzyme preparation, the activity of xylanase is 1000-50000 U / g, the activity of cellulase is 300-3000 U / g, the activity of amylase is 200-1500 U / g, the activity of alpha-galactosidase is 20-200 U / g and the activity of beta-mannase is 200-2500 U / g. According to application of the complex enzyme preparation special for wheat-based diets in poultry feed, 100-300 g of the complex enzyme preparation is added in each ton of the poultry feed. The complex enzyme preparation can effectively degrade soluble xylan and other antinutritional factors, lower the chyme viscosity of the intestinal tract, increase feed metabolic energy, promote nutrition digestion and absorption of animals, improve the growth performance of animals, lower feed cost and increase economic benefits.

The invention relates to the field of gene engineering, in particular to neutral phytase CP53 from a rumen and gene and application thereof. The invention provides the phytase CP53 from a metagenome DNA of the rumen, wherein the amino acid sequence of the phytase CP53 is expressed as SEQ ID NO.1; and the invention provides a genome coding gene cp53 for coding the phytase. The phytase has the following properties that: the optimal pH is 6.5, the optimal temperature is 50 DEG C, and the phytase has good stability when the pH is between 2.5 and 8.5 and high catalytic activity under neutral pH condition. The phytase serving as a novel enzyme preparation can be widely applied to ruminant and aquatic feed industry and the like.