2129 results about "Phytase" patented technology

The present invention provides nucleotide sequences of Aspegillus fumigatus that encode proteins which exhibit enzyme activities. Vectors, expression constructs, and host cells comprising the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes, and methods for modifying the enzymes in order to improve their desirable characteristics. The activities displayed by the enzymes of the invention include those of a tannase, cellulase, glucose oxidase, glucoamylase, phytase, beta-galactosidases, invertase, lipase, alpha-amylase, laccase, polygalacturonase or xylanase. The enzymes of the invention can be used in a variety of industrial processes. Enzymatically active compositions in various forms as well as antibodies to the enzymes and fragments thereof, are also provided.

The present invention relates to enzymes and processes. In particular, there is described an isolated polypeptide comprising the amino acid sequence corresponding to Citrobacter freundii phytase or a homologue, a modified form, a functional equivalent or an effective fragment thereof. There is also described a host cell transformed or transfected with a nucleic acid encoding a bacterial phytase enzyme or a modified form as well as the use of such a phytase or modified form in food or animal feed.

The present invention belongs to the field of poultry feed technology, specifically to a meat large chicken feed and method for preparing the same. The meat large chicken feed is composed of the following components by weight parts: 30-80 parts of corn, 0-30 parts of wheat, 5-40 parts of bean pulp, 0-10 parts of peanut meal, 0-10 parts of cotton meal, 2-8 parts of corn protein powder, 0.5-5.0 parts of lipid, 1.0-6.0 parts of hypo-powder, 0.5-2.0 parts of mountain flour, 0.5-2.0 parts of calcium hydrogen orthophosphate, 0.2-0.4 parts of salt, 0.1-1.0 parts of complex vitamin; 0.1-1.0 parts of complex trace elements, 0.05-0.5 parts of micro-ecological formulation, 0.01-0.2 parts of Chinese herbal medicines additives, and 0.01-0.2 parts of phytase. The inventive meat large chicken feed has high digestibility, nutrient balance, low harmful components, and high security, so that the resistance of broiler chickens is increased and the pollution to the environment is significantly reduced.

The present disclosure relates to an aqueous process for the preparation of a protein isolate and a hydrolyzed protein concentrate from an oilseed meal, optionally comprising:mixing an oilseed meal with an aqueous solvent to form a slurry;optionally treating the slurry with phytase y;separating the slurry with a solid / liquid separation to form:a liquid phase, comprising the aqueous solvent, soluble protein and oil; anda solid phase comprising insoluble protein;separating the liquid phase to form:an oil phase; andan aqueous protein phase;subjecting the aqueous protein phase to membrane filtration to obtain a protein solution; and drying the protein solution to obtain the protein isolatesubjecting the insoluble protein to enzymatic hydrolysis, andsubjecting the hydrolyzed protein to membrane filtration to obtain an amino acid and peptide solution; and drying the amino acid and peptide solution to obtain the hydrolyzed protein concentrate.

The invention relates to a layer foodstuff used for producing health care eggs, consisting of the following components in weight portion: 120 to 160 portions of calcium hydrogen phosphate, 250 to 350 portions of mountain flour, 50 to 60 portions of common salt, 20 to 40 portions of saleratus, 15 to 20 portions of choline, 35 to 40 portions of complex trace elements, 8 to 10 portions of complex vitamins, 10 to 15 portions of lysine, 25 to 30 portions of methionine, 220 to 400 portions of pine needle meal, 1 to 3 portions of bacillus subtilis, 8 to 10 portions of copper sulfate, 2 to 5 portions of phytase, 1 to 5 portions of yeast selenium, 3 to 6 portions of vitamin E, and 250 to 400 portions of Chinese traditional herbal medicine preparation. The invention adds nutritive additives etc. into the foodstuff; therefore the egg laid by the layer contains such beneficial elements as selenium, vitamin E, and is rich in Omega-3 polyunsaturated fatty acid and low in cholesterol.

The invention belongs to the field of feeds, and particularly relates to a six percent-premix for lactating sows and a wheat-type daily ration prepared from the six percent-premix. The premix comprises multivitamins, composite trace elements, DL-methionine, lysine, threonine, L-arginine, valine, composite electrolyte, compound enzyme, 5000 IU of phytase, functional additives, feed attractants, calcium hydrogen phosphate, stone powder, salt, choline, antioxidants and defatted rice bran. On the whole, the daily ration for the lactating sows is capable of protecting the development of mammary glands of the sows, ensuring plentiful milk with high quality in the lactation period of the sows and increasing the survival rate of suckling piglets, and enables the piglets to have higher weaning weight.

The present invention provides for the expression of phytase in transgenic plants or plant organs and methods for the production of such plants. DNA expression constructs are provided for the transformation of plants with a gene encoding phytase under the control of regulatory sequences which are capable of directing the expression of phytase. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or stage and / or tissue specific, depending on the use of the plant or parts thereof. The transgenic plants and plant organs provided by the present invention may be applied to a variety of industrial processes either directly, e.g. in animal feeds or alternatively, the expressed phytase may be extracted and if desired, purified before application.

The invention belongs to the technology field of grain processing, specifically a method for producing active germinated brown rice and products thereof. The method comprises the following steps: screening brown rice raw material to remove impurity, washing, sterilizing, soaking in a nutrient fluid supplemented with calcium lactate, gibberellin, phytase, cellulase and vitamin C for 10-36 hours at 10-40 DEG C, draining, incubating for 10-36 hours at 10-40 DEG C under relative humidity of 50-90% to allow germination, drying with hot air of 40-55 DEG C for 0.5-3 hours until the sprout grows about 0.5-1.2 mm in height, drying with 2-10 w / g microwave for 2-10 minutes until the water content of germinated brown rice is below 15%. Compared with the prior art, the germinated brown rice is nutritious, retains almost all nutrients of brown rice and has high nutritional value.

The invention discloses probiotic type premix feed for laying hens, which is prepared from vitamin compound premix feed for the laying hens in laying period, a bacillus and lactobacilli compound preparation, xylan and mannan complex enzyme, phytase, methionine, lycine, choline chloride, common salt, calcium hydrophosphate, stone dust, fish meal, corn gluten meal, sodium bicarbonate, trace element compound premix feed for the laying hens in the laying period, ethoxyquinoline, bentonite, and rice chaff by mixing in proportion. Compared with the common compound feed for the laying hens, the compound feed prepared by the premix feed reduces discharge of harmful substances such as nitrogen and phosphorus in chicken manure by more than 20 percent, reduces the concentration of harmful gases such as ammonia in a hen house by more than 40 percent, improves the feed utilization rate by 5 to 10 percent and the laying rate by 3 to 5 percent, averagely prolongs the laying period by 25 days, and reduces feeding cost by 5 to 8 percent and the death-cull rate of the laying hens.

The invention discloses a complex enzyme preparation for feeding piglets. The complex enzyme preparation for feeding piglets comprises the following seven enzymes: acid protease, amylase, xylanase, beta-glucanase, cellulase, pectinase and phytase; and the enzymatic activity ratio of the seven enzymes is 1: 1: (8-10): (3.3-4): 1: (0.5-0.55): (0-0.02). By applying the complex enzyme preparation disclosed by the invention, various anti-nutritional factors in feed can be degraded, the viscosity of chyme in an intestinal tract can be reduced, and the metabolic energy of the feed can be improved; the protein and starch digestibility of the piglets can be improved; excessive reproduction of harmful microorganisms in the intestinal tract can be reduced, damage to the intestinal wall can be reduced, and the microecological balance of the intestinal tract can be regulated; the survival rate and disease resistance of the piglets can be improved, the diarrhea rate can be reduced, and the overall uniformity is improved; and the weight gaining of the piglets can be promoted, and the cultivation cost is reduced.

The invention discloses a Chinese medicinal herb additive for egg chickens and application. The Chinese medicinal herb additive comprises membranous milkvetch root, eucommia bark, honeysuckle, jiuyue, Weeping Forsythiae Capsule, szechwon tangshen root, sanguisorba, hawthorn, Indian Buead, officinal magnolia bark, Chinese pulsatilla root, giant knotweed rhizome, ricepaperplant pith, largehead atractylodes rhizome, tangerine peel, malt, indigowoad root, swordlike atractylodes rhizome, indigowoad leaf, three immortals, liquorice, cyrtomium fortune, nutgrass galingale rhizome, chatoyancy, calamus and pinellia tuber, and mixture of the compositions is added with calcium bicarbonate, phytase, vitamin E and methionine. The Chinese medicinal herb additive effectively promotes absorption of nutrient, can prevent and control diseases, strengthen quality of eggshells, reduce the broken egg rate and soft-shell eggs, abnormal eggs and sand shell eggs, can effectively prevent bird flu, prevent and control diseases such as laxness, feather pecking, hair shedding, anus pecking, salpingitis, respiratory disease and the like, can achieve good effect with a dosage of about 4 percent, cannot damage physiological functions of the egg chickens, and can prolong egg laying peak season, improve color of the eggshells and the laying rate by 8 to 20 percent, and increase weight of eggs.

A feed additive composition comprising a direct fed microbial in combination with a protease, and a phytase, and a method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for improving the subject's resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for promoting the growth of beneficial bacteria in the gastrointestinal tract of a subject, which method comprising administering to a subject a direct fed microbial in combination with a protease and a phytase.

The invention relates to a milking sow concentrated feed, mainly comprising the main components such as fat powder, fish meal, puffed corn powder, natural plant extract, lysine, phytase, electrolytic mixture, choline, composite vitamin supplement, salt, rock powder, calcium hydrogen phosphate, mineral elements such as iron, zinc, copper, manganese, iodine, cobalt, selenium, chromium and the like, and accessories such as antioxidant, mildewproof agent, feed carrier and the like. The feed has multiple nutritional components, which can improve synergistically the lactation ability and proliferation ability of milking sows. Meanwhile, the content of the concentrated feed provided by the invention in daily ration is 10 % (according to weight percentage), the concentrated feed is easy to mix evenly and conducive to solve the problems that the methods for preparing feeds and controlling quality are not enough.

The invention discloses freshwater fish feed and a preparation method thereof. The feed comprises the following materials in percentage by weight: 5%-17% of import fish powder, 8%-22% of flour, 3%-16% of potato starch, 3%-18% of beer yeast, 5%-15% of bean pulps, 5%-15% of peanut pulps, 5%-10% of rape seed cakes, 2%-8% of corn powder, 3%-9% of cooked egg protein, 5%-8% of dried meat floss powder, 1%-2% of liquid phytase, 1%-3% of non-starch polysaccharase, 0.5%-1.9% of glutamine, 1%-2% of microecological preparation and 1%-3% of immune polysaccharides. The preparation process comprises a crushing step, a mixing step, a pelletizing step and a spraying step. The freshwater fish feed has the beneficial effects of being capable of well satisfying the nutrition needs of freshwater fish, improving the immunity of the freshwater fish, prompting intestinal absorption and improving the production performance of the freshwater fish.

The invention discloses a laying hen composite premix, and belongs to the technical field of feed composition. A composite enzyme preparation containing beta-mannase, beta-glucanase, acidic proteinase, phytase, pectinase, and amylase is adopted to eliminate the anti-nutrient factors in soybean meal. Yeast cell wall type mycotoxin absorbent is used to effectively eliminate the hazardous factors that may affect the intestine health and are caused by using a large amount of corns. A stomach fortifying acid is used to effectively adjust the pH value of intestines. The used wood chips in the premix are soft and high in fiber content, do not hurt the intestines, do not pollute the environment, can effectively strengthen the digesting and absorbing abilities of laying hens, and have a stronger bearing and water-absorbing performance. The materials mentioned above are systematically and scientifically used to guarantee that the intestines of the laying hens be in the best state. The premix can improve the immunity and disease resistant performance of laying hens, and makes the laying hens exert the maximal laying ability. Moreover, the water content of manure is reduced, and the economic profit of farms that use the premix is prominently improved.

The invention relates to a foodstuff that can enhance the milk yield of lactation period sow, consisting of the following components in weight portion: 250 to 350 portions of calcium hydrogen phosphate, 250 to 350 portions of mountain meal, 100 to 130 portions of common salt, 30 to 40 portions t of choline, 45 to 55 portions of complex trace elements, 8 to 12 portions of complex vitamin, 30 to 50 portions of potassium chloride, 5 to 15 portions of methionine, 15 to 35 portions of lysine, 2 to 8 portions of valine, 50-150 portions of zeolite powder, 15 to 25 portions of medium and short chain fatty acids, 5 to 20 portions of feed attractant, 0.5 to 1 portions of lysozyme, 10 to 25 portions of mycotoxin absorbent, 5 to 7 portions of vitamin E, 2 to 5 portions of phytase, and 3 to 6 portions of chromium Picolinate. The invention adds nutritive additives and mycotoxin absorbent etc. into the foodstuff, thereby obviously intensifying the immunity of lactation period sow, dramatically enhancing the milk yield, bringing forward the oestrous period, and significantly increasing the ablactation weight of the piglet.

The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and / or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.

The present invention provides one kind of improved high specific activity phytase and its coding gene, and the improved phytase has even higher heat stability. The present invention also provides recombinant yeast cell containing the phytase gene of the present invention. The recombinant yeast of the present invention has phytase expressing amount up to 6.5E6 U in each ml of fermented liquid. The phytase of the present invention may be used widely in feed and food industry.

The invention discloses a nonreactive health powdered suckling pig creep compound feed and a preparation method thereof, wherein the feed is prepared from the following materials: corn, broken rice, puffed corn starch, puffed soybean meal, dehulling soybean meal, fermented soybean meal, white fish meal, flour, plasma protein flour, whey powder, fruit sugar, glucose monohydrate, bean oil, calcium formate, calcium biphosphate, salt, enveloped slow release acidulant, choline chloride, suckling pig compound premix, zinc oxide, yeast cell wall polysaccharides, sodium glutamate, arazyme, lipidase, Clostridium butyricum, essential oils, L-lysine hydrochloride, DL methionine, L-threonine, tryptophan and phytase. Based on the physiological characteristics of suckling pig and by the combination of various materials, the feed is supplementary in nutrition and high in design of amino acid and daily ration of energy without adding antibiotics, and Clostridium butyricum and various essential oils are used for protecting the health; therefore, the feed intake of suckling pig can be increased, the weight growth rate is increased, the diarrhea rate after ablactation is reduced, the intestine health of suckling pig can be improved, the immunity is improved, and the survival rate of suckling pig is increased.

The invention relates to a compound enzyme preparation for fermentation of pu'er tea, which is formed by compounding 10% to 20% of cellulase, 10% to 20% of pectinase, 5% to 15% of polyphenol oxidase, 5% to 10% of glucose oxidase, 5% to 10% glucoamylase, 5% to 10% xylanase, 5% to 10% of beta-glucanase, 5% to 10% of beta-mannase, 5% to 10% of tannase, 5% to 10% of acidic protease, 5% to 10% of lipase, 1% to 5% of alpha-amylase and 1% to 5% of phytase. By the aid of coordination of the enzyme system, polysaccharide and other substances in tea including cellulose and hemicellulose can be effectively decomposed, so that various physiochemical substances in cells can be dissolved. Meanwhile, oxidation and condensation of tea polyphenol, decomposition of protein, amino acid and carbonhydrate, a series of reactions of various products including polymerization and condensation and the like are accelerated and promoted.

The invention relates to a marine-fish environmental protective compound feed, consisting of the following materials in weight ratio: 13 to 20 portions of domestic fish powder, 8 to 15 portions of biological fish meal ABP600, 8 to 12 portions of soybean meal, 10 to 18 portions of peanut bran, 3 to 10 portions of corn, 3 to 7 portions of cotton seed meal, 3 to 7 portions of rapeseeds meal, 3 to 5 portions of meat and bone meal, 3 to 5 portionsof squid bowels powder, 20 to 30 portions of high gluten flour, 1.0 to 3.0 portions of blood meal, 1.0 to 2.0 portions of soybean lecithin, 2.0 to 3.0 portions of marine-fish oil, 0.3 to 0.6 portions of choline chloride (50%), 0.05 to 0.1 portions of Vitamin C phosphate (35%), 0.15 to 1.5 portions of calcium biphosphate, 0.2 to 0.5 portions of compound multiplex vitamin, 0.5 to 1.5 portions of compound mineral salt, 0.1 to 0.3 portions of feed attractant, 0.1 to 0.3 portions of micro-bio-preparation, 0.03 to 0.08 portions of A3Alpha peptidoglycan, 0.01 to 0.04 portions of phytase, and 0.04 to 0.08 portions of non-starch polysaccharase. The compound foodstuff can meet the nutrition demands of such high density cultivated marine fishes such as weever, large yellow croaker, American red fish, sparidae fishes, and trachinotus ovatus and rudd etc, and the cultivated objects can rapidly grow and has high foodstuff conversion rate; the compound foodstuff can intensify the immunity and anti-stress ability of fishes and endow them with good vitality, vivid body color and high survival rate; compared with marine fished cultivated by other foodstuffs, the excretion rates of nitrogen and phosphorous of the marine fishes cultivated by the compound foodstuffs is lowered by 10 to 15 percent, and cause smaller contamination to the cultivation water body.

A process for preparing the edible fibre powder from wheat bran by multi-enzyme step treating method includes such steps as treating wheat bran sequentially by endogenous phytase, amylase, proteinase and lipase to obtain high-purity edible fibres, decoloring by sodium hypochlorite and hydrogen peroxide, squeezing, laying aside and superfine pulverizing.

The invention belongs to the animal sitology technical field, in particular to big pig premix and an application method thereof. The premix mainly comprises the following components: iron element, copper element, zinc element, manganese element, iodine element, cobalt element, selenium element, composite vitamine premix, DL-methionine, lysine, threonine, cholinehydrochloride, calcium hydrophosphate, rock flour, salt, carrier and phytase. The big pig premix and conventional basic ration are used to prepare big pig compound feed to feed big pigs with conventional process, and the content of the big pig premix in the big pig compound feed is 4% according to the weight percentage. The big pig premix provided by the invention is simple and safe to use; after feeding big pigs, the fodder-meat rate can be reduced, the big pig growth speed and lean meat percentage are increased and the meat quality safety can be improved.

The invention discloses a feed special for a laying period of a laying hen of a local variety, belonging to the technical field of chicken feed preparation. The feed comprises the following raw materials: 60-65 percent of corns, 2-10 percent of wheat bran, 18-25 percent of bean pulp, 0-1.0 percent of vegetable oil, 4-9 percent of mountain flour, 0.5-1.5 percent of calcium hydrophosphate, 0.2-0.5 percent of table salt, 0.01-0.05 percent of 65 percent lysine, 0.05-0.20 percent of methionine, 0.1-0.15 percent of 50 percent choline chloride, 0.01-0.02 percent of phytase, and 0.5-1 percent of premix. The feed is mainly used specific to the laying period of the laying hen of the local variety; the feed is fed directly in a powder form, so that the feed conversion rate can be effectively improved, the laying peak time can be prolonged, the egg quality can be improved, and the egg broken ratio can be reduced by 3-5 percent.

The invention discloses a preparation method of a broiler feed. The broiler feed belongs to the field of feeds. The broiler feed provided by the invention is prepared from high-quality white corn, bean cake, rape seed cake, cottonseed cake, barley, broomcorn, alfalfa meal, fish meal, mountain flour, plant oil, edible salt, traditional Chinese medicine additive agents, sodium bicarbonate, trace elements, allicin, phytase and vitamin C through the technology of crushing, mixing, granulating, drying and the like. The feed is rich in nutrition, has good mouthfeel, can meet the nutrition need of broilers in a growing period after being taken by the broilers so as to enable broilers to guide qi downward, be good in digestion and be clam, also has the effects of accelerating broilers to grow, and improving the chicken flavor and daily gain, is high in disease resistant capability, and is high in safety.

The invention relates to a general-purpose high-efficiency bulking compound feed for a culter alburnus and a pelteobagrus fulvidraco. The compound feed is prepared from the following raw materials in portions by weight: 13-35 portions of imported steam fish meal, 10-25 portions of imported direct-heat fish meal, 3-5 portions of maggot powder, 2-8 portions of corn protein powder, 5-10 portions of soybean meal, 2-8 portions of fermented soybean, 2-4 portions of soybean meal, 3-8 portions of cottonseed protein, 2-5 portions of peanut meal, 2-5 portions of colza meal, 3-8 portions of meat / bone meal, 2-5 portions of sleeve-fish visceral meal, 23-33 portions of high protein flour, 1.0-3.0 portions of soya bean lecithin, 2.0-5.0 portions of sea fish oil, 0.4-1.0 portion of choline chloride (50%), 0.1-0.3 portion of vitamin C-phosphate (35%), 0.5-1.5 portions of calcium biphosphate, 0.2-0.5 portion of compound multi-vitamin, 0.5-1.5 portions of composite mineral salt, 0.04-0.2 portion of lutein (20%), 0.1-0.3 portion of phagostimulant, 0.1-0.3 portion of micro-ecological preparation, 0.1-0.3 portion of beta-1,3 glucan, 0.01-0.04 portion of phytase and 0.04-0.08 portion of non-starch polyase. The feed can meet all the nutritional requirements of culturing the culter alburnus and the pelteobagrus fulvidraco, so that fish bodies can grow quickly, high feed conversion rate can be achieved, and the coefficient of the feed ranges from 1.2 to 1.4. By using the feed, the immunity performance and the anti-stimulation capability of the culter alburnus and the pelteobagrus fulvidraco can be obviously enhanced, the energy of the fish bodies can be ensured, and the survival rate is improved by 5-10%.

A method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for improving dietary phosphorus absorption and retention, or for improving the efficacy of the phytase, or for improving the subject's resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for reducing populations of pathogenic bacteria in the gastrointestinal tract of a subject, or for reducing nutrient excretion in manure, which method comprising administering to a subject at least one direct fed microbial in combination with a phytase, wherein the phytase is administered to the subject at a dosage of more than about 1500 FTU / kg feed.

Methods and compositions are provided for increasing responsiveness to therapeutic metalloproteases including increasing and / or maximizing responsiveness and preventing botulinum and tetanus toxin resistance due to a functional deficiency of zinc. Also provided are methods for zinc replacement or supplement in lacking individuals comprising the administration of a zinc supplement for a loading period and / or administration of a phytase supplement together with the zinc supplement. Also provided are methods for standardization of botulinum toxin potency assays that provide for greater certainty and margins of safety in the use of products from different manufacturers.