532 results about "Hydrolyzed protein" patented technology

An emulsifying system for elemental diet compositions is provided that comprises a protein source that may be a extensively hydrolyzed protein, free amino acids, short-chain peptides, or a mixture thereof; a lipid source; a carbohydrate source; and an emulsifying system comprising an ocentyl succinic anhydride modified starch and an acetylated monoglyceride emulsifier. The composition may be prepared as a powder or liquid nutritional composition for formulas prepared for infant, pediatric and adult populations in need of an elemental diet. The invention provides a commercially acceptable product in terms of desired stability and physical characteristics and is particularly useful for hypoallergenic infant formula preparations.

Shelf stabilizing agents including hydrolyzed protein, hydrolyzed protein derivatives, and hydrolyzed protein-emulsifier complexes improve the shelf life of a pelletized grain protein-based resin formulation. The pellets may be prepared well in advance of further processing, such as by injection molding and extrusion, which provides shaped articles of the edible or biodegradable variety.

The invention provides a chicken bone protein zymolyte and a chicken essence substrate prepared by the chicken bone protein zymolyte, belonging to the technical field of poultry processing. The invention solves the problems of high cost for producing a chicken essence substrate, limited raw material sources, unavailable effective utilization generally by discarding leftovers, such as chicken bone, and the like, and the like in the prior art. In the invention, a novel chicken essence substrate is developed by adopting the cheap leftovers, namely chicken bone as a raw material and utilizing a biological enzymolysis technology and a hot reaction spice research technology; the chicken essence substrate is prepared by the following steps of: adopting the chicken bone zymolyte obtained after the chicken bone is subjected to double enzymolysis by pancreatic enzyme and Flavourzyme composite flavor protease as a main material, mixing the main material with hydrolyzed vegetable protein (HVP), a yeast extract, glucose, xylose, thiamine, chicken oil and propylene glycol according to a certain proportion; and carrying out Maillard reaction in a high-pressure reactor to prepare the chicken essence substrate with vivid chicken fragrance. The chicken essence substrate has vivid chicken fragrance, rich and strong chicken fragrance and long aftertaste.

A premixing feedstuff of amino acid chelate, which is prepared by mixing a chelating complex of amino acid and iron, a chelating complex of amino acid and copper, a chelating complex of amino acid and manganese, a chelating complex of amino acid and zinc and hydrolyzed protein and a carrier. The feedstuff could be added into various foodstuffs, the adding amount could be 1.0í½2.0úÑ relative of the whole feed. The feedstuff could replace fish meat by 1:1, replace soybean cake by 1:1.5.

The present disclosure relates to an aqueous process for the preparation of a protein isolate and a hydrolyzed protein concentrate from an oilseed meal, optionally comprising:mixing an oilseed meal with an aqueous solvent to form a slurry;optionally treating the slurry with phytase y;separating the slurry with a solid / liquid separation to form:a liquid phase, comprising the aqueous solvent, soluble protein and oil; anda solid phase comprising insoluble protein;separating the liquid phase to form:an oil phase; andan aqueous protein phase;subjecting the aqueous protein phase to membrane filtration to obtain a protein solution; and drying the protein solution to obtain the protein isolatesubjecting the insoluble protein to enzymatic hydrolysis, andsubjecting the hydrolyzed protein to membrane filtration to obtain an amino acid and peptide solution; and drying the amino acid and peptide solution to obtain the hydrolyzed protein concentrate.

The invention relates to a plant nutrient liquid fertilizer. In order to solve the problem of over fertilization, the plant nutrient liquid fertilizer is prepared from the following raw materials in proportion by weight: aqua brassin 12-36, gibberellin 1-4, indoleacetic acid 30-80, abscisic acid 10-20, naphthaleneacetic acid 50-90, salicylic acid 80-180, vitamin C 1-3, cane sugar 40-100, chitin 20-80, 0.05-0.2 percent triacontanol 400-1,000, hydrolyzed protein 2-5, potassium dihydrogen phosphate 300-400, thiourea 40-120, cobalt chloride 5-12, borax 50-100, sodium silicate 30-55, urea 300-500, ammonium molybdate 50-80, copper sulfate 30-65, zinc sulfate 120-200, magnesium sulfate 100-150, calcium chloride 50-120, manganese sulfate 40-80 and water in the amount of 3-8 times the total amount of all the raw materials or water in the amount capable of dissolving all the raw materials, wherein the water is de-ionized water or micro cluster water; and the preparation method is that all the materials are prepared into aqueous solution. The plant nutrient liquid fertilizer has the advantages of favorably solving the problems of over fertilization and shortage of nutrient elements in soil, obviously improving the plant adverse resistance, improving the photosynthesis and the accumulation efficiency of nutritive materials and improving the yield and the quality.

The invention provides an intermediate test method for extracting oil and hydrolyzed protein from peanut by a water enzyme method, which belongs to the technical field of the application of biological technique in food industry. In the method, the peanut is used as a raw material, single alkali protease is used for enzymolysis, a three-phase separator is used to separate oil, oil-water mixture and insoluble residue simultaneously, a disc-type centrifuge is used to further separate the oil-water mixture so as to obtain emulsion and hydrolysate, wherein the emulsion is subjected to freezing, thawing and emulsion breaking to obtain emulsion breaking oil, and the hydrolysate is subjected to spray drying to obtain peanut hydrolyzed protein powder. The invention provides a better process for industrializing the method of extracting oil and hydrolyzed protein by the water enzyme method, and has the advantages of simple technological line and high utilization rate of products.

A process for extracting protein and chitin from fly maggot and preparing chitosan from the chitin incldues such steps as extracting hydrolytic protein by enzyme hydrolysis, removing stink and enhancing fragrance by Maillard reaction, enzymolyzing, removing yellow substances and black spot, etc. The quality of produced protein and chitin conforms with food grade standard.

A process technology using enzymolysis method to extract leftover fish oil from fish processing comprises the following steps: preliminary treatment, in which the leftovers from the fish processing are cleaned and minced; protease hydrolysis, in which neutral protease and papain are hydrolyzed simultaneously; oil-water separation, in which hydrolysate is centrifugally filtered to remove residues, and filtrate undergoes oil-water separation via a centrifuge, thereby obtaining the fish oil and hydrolyzed protein; and fish oil refinement, in which refined fish oil can be obtained after the fish oil is degummed, depickled, decolored and deodorized. The leftovers from the fish processing can be hydrolyzed with the proteases to destroy the associative relation of protein and fat, thereby releasing axunge which undergoes oil-water separation by the tubular centrifuge to obtain the fish oil with high quality, and meanwhile, the protein therein can be fully utilized to produce hydrolyzed protein, so as to change wastes into valuables and synthetically and efficiently utilize the resources of the fish and the processing leftovers.

The invention discloses a method and process for extracting animal micro-molecule polypeptides and amino acids by utilizing a complex enzyme. The method comprises the following steps: (1) crushing animal tissues so that the animal tissues can pass through a sieve of 80-200 meshes, and adding water to prepare a suspension with the concentration of 8-30 percent; (2) raising the temperature to be 35-40 DEG C, regulating the pH value to be 8.0-9.0 by using sodium carbonate, adding a complex enzyme I accounting for 0.5-2.0 percent of the animal tissues, performing ultrasonic enzymolysis at 14-16 kHz for 1-2 hours so that the proteins between cells are subjected to full enzymolysis, regulating the pH value to be 6.5-7.5, adding a complex enzyme II accounting for 0.5-3.0 percent of the animal tissues, fully and uniformly stirring, controlling the temperature of the water bath to be 37-45 DEG C, and performing ultrasonic enzymolysis at 15-17 kHz for 1-6 hours; (3) raising the temperature of the enzymolysis solution to be 90-100 DEG C to inactivate the enzyme for 20-30 minutes after the enzymolysis is finished; (4) filtering the solution subjected to enzymolysis through a 0.22mu m microfiltration membrane; and (5) concentrating the filtrate to the relative density of 1:1.0-1:1.2 to prepare the concentrated solution, wherein the complex enzyme II consists of Alcalase, Flavourzyme, Protamex, neutral protease and papain. According to the method, the enzymolysis efficiency can be improved, and the production cost can be reduced.

This invention relates to a method for the separation of oil, protein, carbohydrates, shell, and minor toxic components from seeds. The oil seed is subjected to a dehulling process to separate out the hull and kernel and the dehulled oil seed is then compressed into flakes under low temperature. The oil seed is then dephenolized and undergo low temperature delintion. In addition, direct hydrolyzation of the oil-complex is carried out. This technology can be used to produce high quality oil and obtain hydrolyzed protein, thereby comprehensively utilizing the oil seed.

A process for preparing hydrolytic protein by using the calcium lime to hydrolyze the residual active sludge includes such steps as proportionally mixing residual active sludge, water and calcium line, stirring, hydrolyzing reaction, filtering, using hydrochloric acid to regulate pH=7.0-8.5, concentrating and drying. Its apparatus is also disclosed.

The invention relates to a low-lactose and a lactose-free milk product and to a process for the production thereof. The lactose in the milk raw material is hydrolyzed, proteins, minerals and sugars are separated into different fractions by the membrane technique, and a low-lactose or a lactose-free milk product is composed from the fractions. The invention provides a milk product, the water therein originating from the original milk raw material. In addition, useful by-products are produced in the process.

The invention is a method for preparing protein polypeptide by continuously hydrolyzing protein with an immobilized enzyme. The process comprises the steps of synthesizing an inorganic carrier for immobilizing protease; immobilizing the protease on the inorganic carrier; preparing an enzymatic hydrolysis reactor which can conduct continuous reaction; and subjecting a protein solution to enzymatic hydrolysis. The method has the advantages of simple process, high operability, mild reaction conditions, recyclable immobilized protease and low economic cost.

The invention relates to a preparation method of a hydrolyzed protein mixture and a protein peptide powder feed additive, wherein a multienzyme complex enzyme method is used for hydrolyzing vegetable protein to prepare the hydrolyzed protein mixture and protein peptide powder. The technology of the method is simple, and a non-starch polysaccharide enzyme and a proteinase are compounded so as to be capable of improving the hydrolyzation rate and degree of protein as well as the utilization rate of feed and reducing the production cost. Products prepared by the preparation method can be used as the feed additive to be capable of significantly improving the laying rate of egg-laying poultry, improving the weight increment of meat poultry, reducing the feed-meat rate and improving the production performance of table poultry as well as reducing the incidence rate of diarrhea in young animals and the feed-meat rate of piggy; and the preparation method is applied to aquatic livestock to be capable of improving the weight increment of fish and reducing the bait coefficient and content viscosity in the intestinal tract.

The invention relates to a test paper tape which can be used in the gynecological multiprogramming dry-chemistry joint detection. The test paper tape provided by the utility model comprises a dry-chemistry multiprogramming detection test paper tape which consists of a plastic substrate tape and various solidified regent blocks, and sample diluent. The dry regent blocks include combinations formed by increasing or decreasing at least three or more regent blocks of a Ph test regent block, a lactic acid regent block, a hydrogen peroxide concentration regent block, a leukocyte esterase concentration regent block, a neuraminidase activity regent block, an amine test regent block, a proline aminopeptidase substrate reagent block, an oxidase substrate reagent block, a N-acetylamine hexosidase substrate reagent block, a trichomonas specific protease substrate hydrolysis reagent block. the gynecological multiprogramming dry-chemistry joint detection test paper test provided by the utility model can detect Ph, lactic acid, hydrogen peroxide, leukocyte esterase, neuraminidase, amine test, proline aminopeptidase, oxidase, N-acetylamine hexosidase and trichomonas specific protease which are contained in leucorrhea sample at the same time, and can accurately reflect the microorganism environment of a women reproductive tract, the cleanness of leucorrhea secretion and the conditions of Candida albicans, trichomonas, gonococcus and the pathogens of bacterial vaginosis, thereby making the gynecological trichomonas detection more comprehensive; besides, the test paper tape provided by the utility model can be used easily and conveniently, and results can be obtained quickly. If the test paper tape is used together with a gynecological dry-chemistry analyzer, the operation can become easier and the result can be obtained more quickly.

A process for extracting oil and hydrolytic protein from peanut by water-enzyme method includes such steps as extracting emulsified oil by use of alkaline proteinase, and demulsifying it by freezing and thawing to obtain free oil while recovering hydrolytic protein powder in the form of micropeptide. Its advantages are high oil output and high nutritive value.

The invention relates to a preparation method of fermented rice sticks, which belongs to the field of food fermentation and comprises a pulp making procedure and a pulp steaming procedure. The preparation method of fermented rice sticks is characterized in that 1-1000ml of debranching amylase for per 100kg of rice is added into rice pulp with the weight percent of 5 to 50% before the pulp steaming procedure and after the pulp making procedure, the pH value is regulated to 4.0-5.0, and enzymolysis is carried out for 30-360 min under the temperature of 30-60 DEG C; 1-50 g of protease with proteolysis peptide bonds for per 100kg of rice is added into the rice pulp, and enzymolysis is carried out for 60-360 min under the temperature of 30-60DEG C and the pH value of 4.0-8.0; then, 0.2 ml / kg of zymophyte is added to the pulp and fermented for 360-3600 min under the temperature of 25-30DEG C and the pH value of 5.0-6.0. In the invention, the debranching amylase is utilized to hydrolyze Alpha-D-1, 6 glycoside bonds as the branched chains in starch and dextrine to generate straight-chain compound sugar containing Alpha-D-1, 4 glycoside bonds so as to obtain the effect on debranching and adding straight chains; the protease with proteolysis peptide bonds can be used for hydrolyzing molecular protein and amino acid to provide sufficient amino acid for following mixed fermentation, shorten fermentation time, and improve the quality and the taste of products.

Dry stabilizing compositions for bioactive materials include sugars and hydrolyzed proteins, and may be formed into tablets or other forms providing enhanced stability for the bioactive material. Compositions containing the bioactive materials may be produced by a method that includes (a) combining the bioactive material with other ingredients in an aqueous solvent to form a viscous slurry; (b) snap-freezing the slurry in liquid nitrogen to form solid frozen particles, beads, droplets or strings; (c) primary drying by water removal under vacuum of the product of step (b) while maintaining it at a temperature above its freezing temperature; and (d) secondary drying of the product of step (c) at maximum vacuum and a temperature of 20 DEG C or higher for a time sufficient to reduce the water activity to below 0.3 Aw.

The present invention relates to cementing operations, and more particularly to improved cement dispersants for cement compositions and methods of cementing. In one embodiment, the present invention provides a method of cementing comprising the steps of: providing a cement composition comprising a cement, and a dispersant composition, the dispersant composition comprising a surfactant and a hydrolyzed protein; placing the cement composition in a desired location; and allowing the cement composition to set.

The invention relates to a new process for preparing animal protolysate containing high calcium with complex enzyme hydrolysized chicken bone mud, which is characterized in that it comprises the following steps: disintegrating chicken bone ultramicroly to make bone mud, formulating bone mud suspending solution, heating under 80-100 Deg .C for 20-30 minutes, cooling to temperature proper for enzyme reaction, regulating pH value, adding complex enzyme for reacting for a certain time, then heating to 85-90 Deg. C and keeping for 10-20 minutes for sterilization, and getting product which is concentration of sepia, semitransparent or stramineous powder. The invention resolves problems of odour of raw material and bitter caused by enzymolysis, the product most of which is short peptide and free amino acid can be adsorbed easily by human body which increase comprehensive utilization ratio of product, and the product possess certain action for inhibiting angiotonin zymose.

The invention relates to a method of breaking yeast cell wall and preparing a yeast extract. Yeast emulsion is used as the raw material, sodium chloride is added into the yeast emulsion, the pH value of the yeast emulsion is regulated, so that the diluted yeast cell solution becomes basic or weakly basic, the yeast cell wall is broken through steam jet continuous sterilization, the wall-broken yeast cell solution is diluted with water to proper temperature and concentration, the pH value of the diluted yeast cell solution is regulated, so that the diluted yeast cell solution becomes basic or weakly basic, and alkaline protease is added into the yeast cell solution to perform autolysis and enzymolysis. The yeast cell solution is subjected to enzyme deactivation, centrifugal separation, evaporation and concentration and blending, so as to produce the yeast extract. The yeast emulsion is used as the raw material, the drying energy consumption of yeast cells is reduced, sodium chloride as the plasmolysis additive is added, in the basic or weakly basic condition, the common wall breaking action of the steam jet continuous sterilization is performed, so that the wall-breaking effect of the yeast cells is good, the dissolution rate of proteins is high, the protease easily acts, the alkaline protease is added, and thus the yield of the hydrolyzed protein and free amino nitrogen is high. The all-breaking rate of the yeast cells is obviously increased, and the content of total nitrogen and amino state nitrogen in the yeast extract is increased.

The invention relates to a coordinated process extraction method of biological active substances in crayfish by-products, belonging to the field of a biological active substance extraction method. The coordinated process extraction method comprises the following steps of: sequentially carrying out enzymolysis treatment on crushed crayfish shells and crayfish heads which are obtained by boiling through water and removing crayfish meat, and taken as raw materials; spraying and drying a filtered solution to obtain hydrolyzed protein powder; carrying out organic acid leaching treatment on a filtered solid, and spraying and drying the filtered solution to obtain organic calcium; adding the filtered solid into ethanol to be treated; carrying out rotary evaporation on the filtered solution to obtain an astaxanthin rough product; and finally, carrying out hydrogen peroxide immersion de-coloring treatment on the filtered solid to obtain de-colored chitin. According to the coordinated process extraction method disclosed by the invention, the crushed crayfish shells and crayfish heads, which are obtained by boiling through the water and removing the crayfish meat, are taken as the raw materials, and a scientific and simple process route is utilized for sequentially obtaining the food-grade standard hydrolyzed protein powder, organic calcium, astaxanthin and chitin products; a production period is shortened, the production efficiency is improved and a product additional value is obviously improved; and a production process is clean production, wastewater emission is reduced and the coordinated process extraction method is good for environmental protection.

The invention provides a preparation method of small molecule peptides of vinasse and application of small molecule peptides of vinasse in skin care products. The preparation method comprises the following steps: S1, carrying out vacuum extrusion, cooling, crushing and sieving on vinasse, the water content of which is 11-22%; S2, carrying out alkali dissolution and acid settlement on the vinasse expanded powder obtained in the S1 to extract vinasse proteins so as to obtain vinasse protein extract; and S3, carrying out hydrolysis on the vinasse protein extract extracted in the S2 with proteolytic enzymes, and carrying out enzyme deactivation, filtration, concentration, freezing and drying to obtain the small molecule peptides of vinasse. By deeply processing the vinasse by adopting a special vacuum expansion technology, the extraction ratio of the vinasse proteins is increased effectively; meanwhile, by adopting the special proteolytic enzymes, the small molecule peptides of vinasse with relatively good moisturizing and repairing functions can be obtained. In addition, the small molecule peptides of vinasse are applied to skin care products, so that the moisturizing and repairing functions of the skin care products are improved while the additional value of the vinasse is increased.

Disclosed are compositions comprising carbohydrate; lipid, comprising from about 0.25% to about 2.5 % lecithin by weight of total lipid; from about 90% to about 99.5% of intact protein by weight of total protein; and from about 0.5% to about 10% of at least one hydrolyzed protein selected from the group consisting of hydrolyzed casein protein and hydrolyzed whey protein; wherein the hydrolyzed protein has a degree of hydrolysis of between about 23% and about 90%, and wherein the compositions are nutritional powders. The nutritional powders provide improved oxidative stability and sensory performance.

The invention discloses a preparation meted for high-stability soybean protein emulsion, and belongs to the field of soybean protein deep processing. The method includes the steps that soybean protein isolate is dissolved in distilled water to obtain a protein solution, the protein solution is preheated, the pH of the preheated protein solution is adjusted, alkaline protease is added for enzymolysis, and the protein solution is freeze-dried after enzymolysis to obtain hydrolyzed soybean protein powder; the hydrolyzed soybean protein powder is dissolved in a phosphoric acid buffer solution to obtain a hydrolyzed soybean protein solution; lecithin is added into the phosphoric acid buffer solution to obtain a lecithin solution; the hydrolyzed protein solution and the lecithin solution are mixed to obtain mixed liquor, sunflower seed oil is added into the mixed liquor, and then homogenization treatment is performed to obtain the high-stability soybean protein emulsion. The compound emulsion prepared through the method is high in emulsion activity and emulsion stability, safe, harmless and suitable for the food industry and other industries. Besides, the method has the advantages that the process is simple, reaction conditions are moderate, and operation is safe.

The invention discloses a method for producing hydrolyzed wheat proteins. The method is characterized by comprising the following steps of: preparing enzymatic hydrolysate, namely adding 1,000 parts of vital gluten in mass for three times according to the weight ratio of the vital gluten to water being 1 to 5-3, after hydrolysis is performed, raising the temperature of the enzymatic hydrolysate to 75-100 DEG C, keeping the temperature of the enzymatic hydrolysate for 3-10 minutes, performing enzyme deactivation on the enzymatic hydrolysate, cooling the enzymatic hydrolysate subjected to enzyme deactivation to 30-35 DEG C, after fermentation is finished, filtering fermented liquid, and finally drying the filtrate by spraying. The method has the advantages that by a multi-enzymolysis and microorganism fermentation combination method, a novel hydrolyzed wheat protein product is developed by fully combining characteristics of a protein structure of wheat gluten proteins, the cleavage sites of protease, biological conversion of microorganisms, animal nutrition absorption and the like, so that the taste and the flavor of the product are greatly improved; the utilization of enzyme preparations can be greatly reduced; the production cost is reduced; the production technology is simple and novel; the period is short; three industrial wastes are not produced; and the hydrolyzed wheat proteins are environmentally-friendly.

A nutrition or other food bar which includes, preferably in moderate to high levels, peptides in the form of hydrated high water activity peptides. The peptides are typically either intact proteins or hydrolyzed proteins. Inclusion of peptides in the form of high water activity peptides helps prevent migration of water from sugars to the proteins which would otherwise result in formation of hard crystalline sugars and food bars which are excessively hard. Whey protein isolate and hydrolyzed whey protein are preferred high water activity peptides.

a peptide product is made by enzymatically hydrolyzing a protein, such as whey protein concentrate, in two stages. A fungal protease is used to hydrolyze the protein in each stage. The hydrolyzed protein is dried to form a peptide product. The peptide product is non-allergenic and lacks the bitter taste that some other peptide products have. If can be used to make milk replacement products and various other food products.