The invention, which belongs to the technical field of substance extraction, relates to a method for extracting high-purity
phospholipid from sleeve-fish-
processing waste. Fresh sleeve-fish-
processing waste is first dried until the
moisture content is less than or equal to 3 percent, and is ground to 10 to 80 meshes; the preprocessed powdery material is then loaded into an extraction kettle, and after the air is removed, extracting
solvent CO2 is injected to set pressure; the supercritical extraction and separation of the extraction
system is regulated as three processes, i.e. extraction, primary separation and secondary separation,
raffinate is taken out of the extraction kettle after triglycercide and
cholesterol are separated and removed,
ethanol which is 4 to 10 times the weight of the
raffinate is added in the
raffinate according to a weight ratio to dissolve the raffinate, clear solution is taken out and concentrated, and is then washed by
acetone, the obtained
solid substance is dissolved in
ethanol and then mixed with water, and precipitate which is obtained by
centrifugation is dried, so that sleeve-fish
phospholipid is obtained. The method has the advantages of less
organic solvent dosage, little environment
pollution, simple
processing equipment, easy operation,
automatic control, stable product quality, low production cost, good quality and high purity.