88 results about "Prepared fish" patented technology

The invention relates to fish bone charcoal adsorbent, a preparation method thereof and application to heavy metal waste water. The preparation method includes the following steps of 1 impurity removing, wherein an obtained fish bone raw material is stirred and scalded in water to remove impurities; 2 drying, wherein the fish bones are put into a drying oven to be dried after the impurities are removed; 3 pulverizing, wherein the dried fish bones are pulverized in a pulverizer, and obtained fish bone dust serves as an adsorbent raw material; 4 high-temperature roasting, wherein the prepared fish bone dust is roasted and carbonized at high temperature, naturally cooled to the room temperature and then taken out to obtain the fish bone charcoal adsorbent. The preparation method has the following advantages that fish processing waste is low in price and easy to obtain, as the fish processing waste is prepared into fish bone charcoal, the potential value of the fish processing waste can be developed further, and the environmental pollution caused by discarding the fishing processing waste can be effectively reduced. In addition, the prepared bone charcoal is high in stability and adsorbability, easy to regenerate after absorption and reusable.

The invention relates to a preparation method of fish skin antifreeze protein, fish skin antifreeze protein prepared by adopting the method, and applications of the fish skin antifreeze protein. The method comprises the steps of beating fish skins into fish skin paste, mixing the fish skin paste with water and then milling into fish skin paste liquor; centrifuging the fish skin paste liquor to remove the upper-layer fat to obtain defatted fish skin paste liquor; performing ultrasonic treatment to the defatted fish skin paste liquor; mixing the ultrasonically-treated defatted fish skin paste liquor with compound protease for performing enzymatic hydrolysis; preserving heat, cooling and centrifuging, and collecting supernatant; micro-filtering the supernatant to obtain fish skin protein liquor; condensing and freeze-drying the fish skin protein liquor to obtain the fish skin anti-freeze protein. Aiming at the current development situation of the existing antifreeze protein products, the invention discloses a simple and efficient preparation method for fish skin antifreeze protein by taking fish skins as raw materials through a great amount of experiments; the prepared fish skin antifreeze protein has better specific anti-freeze function, easily realizes the industrial production, thus having tremendous application prospects and market values.

The invention relates to a preparation method of high-melt-temperature fish scale gelatin. The preparation method comprises the following steps: by taking waste scale generated during processing of freshwater fish as a raw material, performing low-acid de-calcification, ultrasonic-microwave extraction, centrifugation, ultramembrane filtration, bio-enzyme modification and spray drying technologies, wherein the prepared fish scale gelatin product has higher extraction rate and melt temperature. The preparation method has the advantages that the fish scale gelatin extracted by adopting the ultrasonic-microwave technology has higher extraction rate; the content and purity of protein in the fish gelatin can be increased by adopting the ultramembrane filtration technology; and the melt temperature of the fish gelatin can be increased by adopting the bio-enzyme modification technology. The method provides a new conception for the production of high-melt-temperature fish scale gelatin, and provides a new method for the modification and industrial production of the fish gelatin.

The invention provides a method for extracting fish collagen from fish skin. The method comprises the following steps: (1) extracting collagen: adding protease to the fish skin to carry out enzymolysis, then killing the protease to obtain a collagen crude extracting solution; (2) decolorizing: adding pure active carbon to the prepared collagen crude extracting solution from the step (1) while carrying out stirring, then carrying out decolorizing, deodorizing and filtering to obtain a collagen clear solution; (3) removing a heavy metal: carrying out absorbing for the resulting collagen clear solution from the step (2) through a resin for removing arsenic, then washing the resin for removing the arsenic through purified water and collecting eluent; (4) finishing the product: carrying out evaporating, condensing, spray drying for the resulting eluent from the step (3) to obtain the fish collagen having a molecular weight of 2000-3000 dalton. With the present invention, the process for the fish collagen is simple; product quality of the fish collagen is good; toxic and harmful reagents are not remained in the prepared fish collagen product; the heavy metal in deep sea fishes can be removed well so as to effectively control the product quality and provide high safety.

The present invention relates to matter extraction from animal material and is the extraction process of tetrodotoxin. The present invention provides the technological method of raising yield of tetrodotoxin. The technological process includes the steps of: cutting internal organs of globefish, grinding into slurry, press filtering, heating to eliminate protein and oil, filtering to obtain clear liquid, concentration, elution of crystal inchromatographic column to obtain pure tetrodotoxin product.

The invention discloses a core sea cucumber fish ball and a preparation method thereof. The fish ball comprises an inner core and a sea cucumber external layer according to a mass ratio of 1:1-4, wherein the sea cucumber external layer comprises, by mass, 20-40 parts of surimi, 3-15 parts of chicken breast, 3-12 parts of water, 0.5-15 parts of sea cucumber, 9-20 parts of edible starch, 0.6-2 parts of salt, 0.6-2 parts of monosodium glutamate, 0.4-1 part of white sugar, and 0.1-0.18 parts of sodium tripolyphosphate. The preparation method of the core sea cucumber fish ball of the invention comprises the following steps: cleaning the sea cucumber, dicing, mix-beating, chopping, adding above flavorings, preparing the internal core, molding by a core fish ball molding machine, boiling with water, cooling, quickly freezing, packaging, and warehousing. According to the invention, the external layer which adopts the mix-beating of the sea cucumber and the surimi makes the mouthfeel of the external layer be crisp and refreshing and the prepared fish ball have the advantages of good mouthfeel and abundant nutrition. The preparation of the core sea cucumber fish ball of the invention has the advantages of simplicity and high production efficiency.

The invention relates to a method for extracting fish collagen, and belongs to the technical field of natural protein extraction. Through adoption of a fish collagen extracting process in which an organic solvent is not used, removal and separation of fat are achieved. The invention adopts the technical concept as follows: firstly, through grinding and homogenizing under an ultrasonic action, tissue cells of fish skin are completely broken, and during subsequent alkali treatment and enzymatic treatment, fat and protein can be more removed, so that a crude extract with low fat content can be obtained; then, a crude extract solution is enabled to pass through a resin bed obtained after lipophilic modification, so that the purpose of adsorbing low-content lipids is achieved. The prepared fish collagen has the advantages of high purity, low fat content and good water solubility.

The invention discloses a production method of fish collagen peptide chelated calcium. The production method comprises the following steps: carrying out pretreatment and proteolysis on fish bone, fish skin or fish scale which serves as raw materials, then carrying out chelation reaction on the raw materials and calcium under the gradient acidic pH condition, centrifuging and carrying out nanofiltration to remove the non-chelated calcium under the alkaline condition to obtain the fish collagen peptide chelated calcium with high content of calcium. The production method of the fish collagen peptide chelated calcium is simple and convenient in extraction process and low in cost, and is applicable to industrial production; the prepared fish collagen peptide chelated calcium has a good function of promoting absorption of the calcium.

The invention relates to the technical field of medical instruments, and in particular relates to an aquatic product fish skin gelatin sponge and a preparation method thereof. The aquatic product fish skin gelatin is used as a main material, chitosan is used as an auxiliary material, and glutaraldehyde is used as a cross-linking agent; the raw material liquids of different proportions are subjected to freeze drying to prepare fish skin gelatin sponges with different properties; the preparation process of the sponge is simple; the sponge is low in cost and readily available; some defects of the conventional collagen sponge are overcome. The prepared fish skin gelatin sponge has a uniform porous netlike microstructure and higher mechanical strength and good moisture absorption and is a better biomedical material.

The invention discloses a method for preparing fish egg sugar protein peptide with an anti-osteoporosis effect. The method comprises the following steps: performing freeze drying on the fish eggs, and performing ungrease treatment by using ethanol; performing freeze drying on the fish eggs subjected to ungrease treatment, adding protease for hydrolyzing, regulating pH value of the hydrolysate into neutral after the hydrolysis is ended, performing ultrafiltration and desalting the supernatant fluid of the centrifuged hydrolysate, concentrating and drying to obtain the fish egg sugar protein. The prepared fish egg sugar protein peptide can be used for preparing health-care food, functional food or medicines for preventing and treating osteoporosis. The used raw materials are rich and readily available, the product can perfectly keep the structure of sialic acid in the fish egg sugar protein, and the method is simple in process steps, low in equipment investment, free in environmental pollution and suitable for industrial production. The product has high anti-osteoporosis activity and can be develop the food or medicines with the effects of safely and effectively preventing and treating osteoporosis; and meanwhile, the defects of the development and utilization of fish egg sugar protein are overcome, and a novel way for high-valued processing of fish eggs is developed.

The invention provides a production method for a fish oil microcapsule product with ultrafine edible fungus powder as a main wall material. Main materials for microcapsule encapsulation are ultrafine mushroom and tremella powder and perilla oil used as a deodorization substance for fish oil. The production method comprises the following steps: preparation of the ultrafine mushroom and tremella powder; preparation of composite fish oil; and preparation of a fish oil microcapsule. The prepared fish oil microcapsule product has a pure white color and good appearance and can effectively encapsulate a core material. The product has strong antibacterial activity, effectively inhibits fishy smell of the fish oil, supplements and reinforces the function of unsaturated fatty acid of the fish oil and has the excellent characteristics of water solubility, thickening performance, gelatinization, film formation, etc. The wall material of the fish oil microcapsule becomes a nutrition component for the product and enriches nutritional functions of the fish oil product.

The invention discloses a method for preparing freshwater fish bone gelatin by an enzyme process. The method orderly comprises the steps of: preparing a fish bone by enzymolysis of fish, pickling and desalting, carrying out enzymolysis, washing, extracting gelatin, centrifuging, filtering, concentrating and drying. Compared with the prior art, the method has the advantages that (1) a liming process in the traditional process is replaced by the enzyme process, thus the production period is greatly saved, and industrial wastewater is reduced; (2) the fish is removed by a protease hydrolysis process, thus the fish removal efficiency is high and thorough, and the obtained fish bone is smooth and clean; (3) ossein is preprocessed by adopting papayotin, the point at which the pH value is close to a neutral state is an isoelectric point of collagen, collagen loss is reduced, no unmanageable acid hydrolysis liquid is generated, and the generated collagen is safe and reliable; (4) the viscosity of the prepared fish bone can be up to 3.5mPa.s, and the fish bone gelatin is an excellent material for preparing medicinal capsules; and (5) the gelatin prepared from the fish bone is safer than animal gelatin, and the processing added-value of the fish product can be improved.

The invention provides a fish digestion-resistant peptide with an oxidation resisting function. The fish digestion-resistant peptide has the amino acid sequence shown in SEQ ID NO: 1-11. The fish digestion-resistant peptide is prepared through hydrolyzing fish protein by using an animal proteolysis compound enzyme, carrying out centrifugation, and extracting supernatant; and carrying out ultrafiltration on the supernatant by using an ultrafiltration membrane with the molecular weight cutoff of 3,000Da, collecting filtrate, and then, carrying out concentrating and freeze-drying. The fish digestion-resistant peptide with the oxidation resisting function, provided by the invention, has relatively good free-radical removing capacity and can still maintain very good oxidation resistance after the fish digestion-resistant peptide is subjected to digestion and absorption by a gastrointestinal tract; and furthermore, natural fishes serve as a raw material, so that the prepared fish digestion-resistant peptide is safe and non-toxic. According to the fish digestion-resistant peptide, the preparation method is simple, the problem in the industrialized production of the fish digestion-resistant peptide can be effectively solved, and thus the fish digestion-resistant peptide has relatively high social and economic benefits.

The invention discloses a method for preparing fish sauce through rapid fermentation of gonads of sea cucumbers. The method comprises steps as follows: (1), the gonads of the sea cucumbers are mashed to form paste, 100-120 U / g of protease is added, and the mixture is hydrolyzed for 3-5 days; (2), koji is inoculated for fermentation, and the mixture is fermented for 40-50 days; (3), exogenous fish viscera are added for fermentation; (4), yeast is added to a fermentation broth in the step (3), and the mixture is fermented for 40-50 days; (5), the fermentation temperature in the step (4) is reduced to 20-25 DEG C, and the mixture is fermented for 3-4 months; (6), the fermentation broth in the step (5) is sterilized and canned, and a finished product is obtained. In combination of a conventional fish sauce fermentation technology, improvement is performed; the whole fermentation process is in a low-salt state, and the stinking smell problem difficult to solve during preparation of the fish sauce is solved. According to the method, the fermentation time is short, and the prepared fish sauce tastes unique and has rich nutrition and certain health-care functions.

The invention relates to the aspect of aquaculture, in particular to a formula and production process of an environment-friendly and pollution-free grass carp feed. The formula includes a component A, a component B and a component C. According to the mass parts, 1-40 parts of bean pulp, 1-30 parts of peanut meal, 5-15 parts of wheat meal, 5-15 parts of corn flour and 5-10 parts of fish oil are evenly mixed to form the component A, 15-30 parts of a fermented feed, 10-25 parts of clostridium alcohol protein, 10-30 parts of chicken meal, 5-10 parts of earthworm powder, 1-5 parts of a liver and bile protecting additive and 1-5 parts of a decavitamin additive are evenly mixed to form the component B, and 20-30 kg of pulverized corn straw is put into 100 kg of starch water to be heated until viscosity is realized to form the component C. The raw material ratio in the process conforms to the feeding habit characteristic of grass carps, the production process is environmentally friendly and free of pollution, and the prepared fish feed cannot contaminate water.

The invention relates to a method for extracting tetrodotoxin from globefish internal organ tissues and then primarily purifying the tetrodotoxin. The globefish internal organ tissues are subjected to homogenate, dialysis, and reversed-phase weak anionic chromatography to remove the impurities, then tetrodotoxin is condensed and purified by a weak cationic exchange technology, and finally the tetrodotoxin is subjected to freeze drying so as to obtain the tetrodotoxin dry powder with a purity of 80%. In the provided method and technology, LC-MS or LC-MS / MS is used to detect the tetrodotoxin concentration in each step so as to guarantee that no tetrodotoxin is lost or discarded. The tetrodotoxin yield of the provided method is 10 to 100 times more than that of conventional methods recorded in literature and current patents. The provided technology especially the continuous fluid and special weak cationic exchange materials can process 10 kilograms of globefish ovary at one time, and is capable of obtaining 10 to 20 grams of tetrodotoxin dry powder with a purity of 80% to 90%.

The method for extracting tetrodotoxin from liver of globefish includes the following steps: cutting liver of globefish, soaking at low temp., stirring, filtering, freezing, repeatedly soaking for several times to obtain crude venom, freezing and standing still, precipitating, heating and boiling, discharging out venom, freezing, and separating out supernatant venom, regulating pH value, adding active carbon, stirring, filtering, mixing cation 110 resin and said clear liquor, stirring, filtering, making said clear liquor pass through cation 110 resin, saturating and using acid to make elution, collecting eluant, regulating pH value, cooling, standing still, crystallizing, sucking out mother liquor, filtering, more regulating pH, freezing, standing still, crystallizing and repeatedly regulating pH for several times to obtain white powder crystal.

The invention discloses a fish protein fertilizer capable of promoting the growth of plants and a preparation method of the fish protein fertilizer. The fish protein fertilizer comprises the followingcomponents in parts by weight: 65-85 parts of fish proteins, 4-6 parts of methionine, 3-5 parts of tryptophan and 15-20 parts of modified chitosan. The preparation method of the fish protein fertilizer comprises the steps: firstly, adding methionine and tryptophan into the fish proteins so as to be premixed, and carrying out grinding and sieving to obtain protein powder with uniform particle size; secondly, adding the protein powder into a ball mill, adding water serving as a medium water and modified chitosan, and adsorbing the protein powder into the modified chitosan by mechanical mixing to obtain a wet product of the fish protein fertilizer; and thirdly, placing the wet product of the fish protein fertilizer into a vacuum drying oven, and carrying out drying to obtain a finished product of the fish protein fertilizer. The prepared fish protein fertilizer is free of salts and heavy metal residues, high in nitrogen content, good in water solubility and long in shelf life, can be widely applied to operation of fertilizer application to leaves and root systems of plants and plays a role in actively promoting the growth of crops.

The invention discloses a method for preparing minced fish sausages with fish skin gelatin. The method comprises the steps of preparing fish skin gelatin protein powder by adopting a hot extraction method, then preparing a fish skin gelatin protein solution, preparing minced fish, and finally adding the prepared fish skin gelatin protein solution to the minced fish to prepare sausages, thus obtaining the minced fish sausages with the fish skin gelatin. The fish sausages are prepared from the minced fish serving as a processing byproduct of fish as a main raw material and adding the gelatin extracted from the fish skin byproduct, so that the method is simple and low in cost and can be used for improving the additional value of the fish processing byproducts; and the fish skin gelatin protein substitutes vegetable protein, so that the nutritional value and the quality of the sausages are improved. The fish sausages prepared by adopting the method are complete in shape, elastic, compact in section holes and good in mouthfeel and flavor.

The invention discloses a composite fish oil microcapsule and a preparation method thereof. According to the preparation method, prepared fish oil, algae oil, tea seed oil, kaga oil, vitamin C and vitamin E are compounded, and a mixed oil core material is prepared; chitosan and Arabic gum are selected and dissolved in hot water, and a wall material solution is prepared; the wall material solution is added to the mixed oil core material in proportion, is homogenized and has a condensation reaction, and a composite condensation liquid is prepared; the composite fish oil microcapsule is prepared through spray drying of the composite condensation liquid. According to the preparation method, a process is reasonable, the preparation is simple, raw materials are natural, the use amount is appropriate, and the production cost is low. The prepared composite fish oil microcapsule has a smooth surface, uniform particle diameters, comprehensive nutrition, remarkable efficacy and a long guarantee period and doesn't have fishy smell.

The invention provides a preparation method of a fish protein peptide powder. The preparation method comprises the following steps: adding protease into fish meat paste so as to carry out enzymolysis, performing enzyme denaturalization after enzymolysis, further performing centrifugation and microfiltration by using a ceramic film so as to obtain a fish protein peptide liquid, and drying the fish protein peptide, thereby obtaining the fish protein peptide powder, wherein the protease is selected from one or more of neutral protease, papain and pancreatin; and the addition amount of the protease is 400-1500 IU / g. The preparation method is simple in process, easy to operate and beneficial to industrial production; and the produced fish protein peptide is administrated in a rubbing manner, and has a function of promoting heal of scalded skin when the dosage is 50-150mg for each wound surface.

The invention relates to a method for producing a carbon adsorption material with fish scales (carps). The method is as follows: (1) after being aired, fish scales which are taken as a raw material (not required to be ground) are uniformly mixed with a certain activator (KOH or H<3>PO<4> or ZnCl<2>) (the weight ratio of the activator to the fish scales is (1.5 to 3):1) and steeped for 10 to 24 hours; (2) after the steeped material is activated under the high temperature of 500 DEG C to 850 DEG C (potassium hydroxide is within a range from 800 DEG C to 850 DEG C; and phosphoric acid or zinc chloride is within a range from 500 DEG C to 600 DEG C, not under inert gas shielding ) for 30 to 90 minutes, an activated fish scale material is prepared; (3) the obtained activated scale material is washed with water (the activator is recovered) to be almost neutral (pH value is 6 to 8), and after drying, the finished scale carbon adsorption material is prepared; (4) the specific surface area of the prepared fish scale carbon adsorption material reaches 530m<2> / g to 992m<2> / g, and the methylene blue adsorption value is 128mg / g to 227mg / g. According to the invention, the carbon adsorption material which is prepared with the fish scales as raw materials can replace a variety of applications of active carbon for adsorption, helps realizinge the utilization of fish scale waste as a resource, and helps save resources such as timers, coal and coconut shells.

The invention provides a preparation method of fish fin tissues for extracting genomic DNA (deoxyribonucleic acid) of fish, relating to a preparation method of animal tissues for extracting the genomic DNA (deoxyribonucleic acid). The preparation method is used for solving the problems that an existing preparation method of a fish fin tissue sample of the fish requires high preservation conditions, and is unfit for field sampling and the carrying is inconvenient. The preparation method comprises the following steps: (1) collecting the fresh fish fin tissues; (2) flatly putting the fish fin tissues on filter paper, and covering the fish fin tissues by another piece of filter paper; (3) putting the fish fin tissues covered by the filter paper at a cool and ventilation place until the sample is totally dried; (4) carrying out damp-proof preservation on the sample under the room temperature. The preparation method has the advantages that the prepared fish fin tissue sample can be preserved for a long time under the normal temperature and does not need a low-temperature environment, so that the occupied space is reduced, and the preservation cost is lowered; the operation process is concise, the sample is convenient to carry, the workload can be reduced particularly when the quantity of the samples is relative large, the working efficiency is increased, and meanwhile, the experimental quality is guaranteed.

The invention relates to an ethyl ester type fish oil refining processing technology, which specifically comprises: S1, pre-treating crude fish oil, wherein an adsorbent is added to crude fish oil, the crude fish oil is stirred and adsorbed by a thermomagnetic stirrer, thermal insulation stirring is performed for 30-40 min, the fish oil is cooled to a room temperature, the cooled fish oil is filtered to remove the adsorbent, and the obtained fish oil is used for spare; S2, carrying out a pre-esterification reaction, wherein the prepared fish oil is pumped into a reaction kettle, a uniformly-mixed mixture of an acid catalyst and ethanol is added to the reaction kettle, condensation water is introduced while stirring is performed, and the acid catalyst can catalyze the pre-esterification reaction of the fish oil under an ethanol reflux state to reduce the acid value of the fish oil; S3, carrying out an esterification reaction, wherein the fish oil obtained by the pre-esterification reaction in the step S2 is pumped into a reaction distillation tower, and an esterification reaction for fish oil refining and distillation concentration are synchronously performed in the reaction distillation tower, and the esterification reaction is an enzyme and alkali combined catalytic reaction; and S4, carrying out freeze decolorization and deoxidation on the fish oil prepared by the esterification reaction in the step S3 to obtain the ethyl ester type fish oil.

The invention discloses a method for refining tilapia oil, which comprises the following steps: by using tilapia processing waste fish heads as the raw material, shredding, hydrolyzing with compound proteinase under certain conditions, centrifuging to obtain crude fish oil, and decolorizing the crude fish oil with atlapulgite under certain conditions to obtain the refined fish oil. The crude fish oil yield is 12-13g per 100g of fish head; the fish oil extraction is simple to operate and low in cost; and the obtained product has favorable product quality. After the crude fish oil is decolorized and subjected to other refinement treatment, the prepared fish oil is clear and transparent light yellow in color, and the oil quality achieves the refined fish oil primary standard except the iodine value.

The invention relates to a method for preparing a fish-skin collagen powder by the use of dry fish skin. The method comprises the following steps: dry fish skin is selected and is immersed in water for two times, and edible hydrochloric acid is added during the second immersion so as to remove impure protein on the surface of the fish skin; the fish skin is washed with water to neutral pH; drinking water and edible vinegar are added after crushing, and then ultrasonication and deodorization treatments are carried out; papain is added to carry out controlled-enzymatic hydrolysis so as to obtain a fish-skin enzymatic hydrolysate; activated clay and a flocculating agent are added after enzyme deactivation, static settlement is carried out, and hydroxyapatite impurities in the fish skin are removed; and a supernatant is taken and active carbon is added for decarburization clarification, and filtration, concentration and spray drying are successively carried out to obtain the fish-skin collagen powder. The method provided by the invention has advantages of advanced technology, reasonable process and controllable operation, and is suitable for large-scale production. By the method, the prepared fish-skin collagen powder has no fishy flavor and has high purity.

The invention discloses a fish oil preparation technology, comprising the following preparation procedures: strictly selecting and storing raw materials to ensure the activity of the raw materials in the whole fish oil preparation procedures; then performing steps of pulping, hydrolysis, salting out, purification and the like, and strictly controlling the temperature, a pH value and a procedure of each step, so that the fish oil preparation technology becomes simple and feasible. The prepared fish oil can meet usage requirements in a pharmacopoeia and other industries and is suitable for mass production of a general manufacturer. The fish oil preparation technology provided by the invention solves a problem of cumbersome fish oil preparation procedures in China at present.

The invention discloses a method for improving the fermentation quality of low-salt fish sauce by using mixed strains of planococcus, and belongs to the technical field of food microbiology. The production cycle of the fish sauce fermented by using the mixed strains of planococcus (the deposition numbers are CGMCC NO.17057, CGMCC NO.17058, CGMCC NO.17059 and CGMCC NO.17060 correspondingly) is greatly shortened, and the fermentation temperature is reduced. The fish sauce obtained by the method is transparent brownish red, with the unique aroma of aquatic products, and without impurities such assuspension or flocculent. The finished products of the fish sauce are low in salt content and high in amino acid nitrogen content (according to the Chinese fish sauce industry standard, the fish sauce is classified as first-grade fish sauce), the content of volatile basic nitrogen and histamine are lower than the national standard, the prepared fish sauce is delicious and of good quality, and a more stable, safer and more suitable mixed leavening agent is provided for the fermentation of fish sauce.

The invention provides a preparation method of nutritional fish sausage. The method comprises the following production steps: (1) emulsion paste is prepared for use; (2) fish paste is prepared for use; (3) the emulsion paste is added to be stirred; (4) the fish sausage is formed; (5) gelling is performed; (6) high-temperature sterilization is performed; (7) after drying and packaging, the product is prepared. Through relevant destructive test, the expiration date of the prepared product reaches 9 months; all used raw and auxiliary materials conform to the corresponding quality standards; added food additives conform to the requirements of GB 2760 standards; the produced product conforms to the standard requirements; the eating is safe. The prepared fish sausage has the advantages that various kinds of sauce are added, so that the nutrition is more balanced and rich and can be easily absorbed; the fish sausage is very suitable for various types of user groups; different sauce can form sandwiches in different colors, so that the tangent plane of the product is very beautiful and bright; the product is elastic, brittle and smooth; the nutritional fish sausage can be sold at normal temperature; the expiration date is long.

The invention relates to a preparation method of a fish bait for resisting marine pathogenic vibrios. The preparation method comprises the following steps: heating and steaming glutinous rice, adding ethanol with a mass concentration of 30%-70% while the glutinous rice is hot, and controlling a mass volume ratio of the glutinous rice to the ethanol to be (1:0.01)-(1:0.05) g / mL; uniformly mixing and cooling, sequentially adding rape seed cake powder and a fructus corni extracting solution in proportion, and uniformly stirring, chopping and drying to obtain the fish bait; wherein a mass ratio of the glutinous rice to the rape seed cake powder is 1:1 to 1:10, and a mass volume ratio of the glutinous rice to the fructus corni extracting solution is 1:0.1 to 1:10 g / mL. The fructus corni is taken as a raw material, a technology for extracting a fructus corni phenolic acid compound is optimized by adopting a Box-Behnken response surface method, the yield of the fructus corni phenolic acid compound is improved, and an activity of the extract in resisting marine pathogenic bacteria is improved. The prepared fish bait is environmentally friendly, safe, reliable and capable of effectively resisting marine pathogenic bacteria, and has high industrialization value.