88 results about "Prepared fish" patented technology

The invention relates to a preparation method of fish skin antifreeze protein, fish skin antifreeze protein prepared by adopting the method, and applications of the fish skin antifreeze protein. The method comprises the steps of beating fish skins into fish skin paste, mixing the fish skin paste with water and then milling into fish skin paste liquor; centrifuging the fish skin paste liquor to remove the upper-layer fat to obtain defatted fish skin paste liquor; performing ultrasonic treatment to the defatted fish skin paste liquor; mixing the ultrasonically-treated defatted fish skin paste liquor with compound protease for performing enzymatic hydrolysis; preserving heat, cooling and centrifuging, and collecting supernatant; micro-filtering the supernatant to obtain fish skin protein liquor; condensing and freeze-drying the fish skin protein liquor to obtain the fish skin anti-freeze protein. Aiming at the current development situation of the existing antifreeze protein products, the invention discloses a simple and efficient preparation method for fish skin antifreeze protein by taking fish skins as raw materials through a great amount of experiments; the prepared fish skin antifreeze protein has better specific anti-freeze function, easily realizes the industrial production, thus having tremendous application prospects and market values.

The invention provides a method for extracting fish collagen from fish skin. The method comprises the following steps: (1) extracting collagen: adding protease to the fish skin to carry out enzymolysis, then killing the protease to obtain a collagen crude extracting solution; (2) decolorizing: adding pure active carbon to the prepared collagen crude extracting solution from the step (1) while carrying out stirring, then carrying out decolorizing, deodorizing and filtering to obtain a collagen clear solution; (3) removing a heavy metal: carrying out absorbing for the resulting collagen clear solution from the step (2) through a resin for removing arsenic, then washing the resin for removing the arsenic through purified water and collecting eluent; (4) finishing the product: carrying out evaporating, condensing, spray drying for the resulting eluent from the step (3) to obtain the fish collagen having a molecular weight of 2000-3000 dalton. With the present invention, the process for the fish collagen is simple; product quality of the fish collagen is good; toxic and harmful reagents are not remained in the prepared fish collagen product; the heavy metal in deep sea fishes can be removed well so as to effectively control the product quality and provide high safety.

The present invention relates to matter extraction from animal material and is the extraction process of tetrodotoxin. The present invention provides the technological method of raising yield of tetrodotoxin. The technological process includes the steps of: cutting internal organs of globefish, grinding into slurry, press filtering, heating to eliminate protein and oil, filtering to obtain clear liquid, concentration, elution of crystal inchromatographic column to obtain pure tetrodotoxin product.

The invention discloses a core sea cucumber fish ball and a preparation method thereof. The fish ball comprises an inner core and a sea cucumber external layer according to a mass ratio of 1:1-4, wherein the sea cucumber external layer comprises, by mass, 20-40 parts of surimi, 3-15 parts of chicken breast, 3-12 parts of water, 0.5-15 parts of sea cucumber, 9-20 parts of edible starch, 0.6-2 parts of salt, 0.6-2 parts of monosodium glutamate, 0.4-1 part of white sugar, and 0.1-0.18 parts of sodium tripolyphosphate. The preparation method of the core sea cucumber fish ball of the invention comprises the following steps: cleaning the sea cucumber, dicing, mix-beating, chopping, adding above flavorings, preparing the internal core, molding by a core fish ball molding machine, boiling with water, cooling, quickly freezing, packaging, and warehousing. According to the invention, the external layer which adopts the mix-beating of the sea cucumber and the surimi makes the mouthfeel of the external layer be crisp and refreshing and the prepared fish ball have the advantages of good mouthfeel and abundant nutrition. The preparation of the core sea cucumber fish ball of the invention has the advantages of simplicity and high production efficiency.

The invention discloses a production method of fish collagen peptide chelated calcium. The production method comprises the following steps: carrying out pretreatment and proteolysis on fish bone, fish skin or fish scale which serves as raw materials, then carrying out chelation reaction on the raw materials and calcium under the gradient acidic pH condition, centrifuging and carrying out nanofiltration to remove the non-chelated calcium under the alkaline condition to obtain the fish collagen peptide chelated calcium with high content of calcium. The production method of the fish collagen peptide chelated calcium is simple and convenient in extraction process and low in cost, and is applicable to industrial production; the prepared fish collagen peptide chelated calcium has a good function of promoting absorption of the calcium.

The invention relates to the technical field of medical instruments, and in particular relates to an aquatic product fish skin gelatin sponge and a preparation method thereof. The aquatic product fish skin gelatin is used as a main material, chitosan is used as an auxiliary material, and glutaraldehyde is used as a cross-linking agent; the raw material liquids of different proportions are subjected to freeze drying to prepare fish skin gelatin sponges with different properties; the preparation process of the sponge is simple; the sponge is low in cost and readily available; some defects of the conventional collagen sponge are overcome. The prepared fish skin gelatin sponge has a uniform porous netlike microstructure and higher mechanical strength and good moisture absorption and is a better biomedical material.

The invention discloses a method for preparing fish egg sugar protein peptide with an anti-osteoporosis effect. The method comprises the following steps: performing freeze drying on the fish eggs, and performing ungrease treatment by using ethanol; performing freeze drying on the fish eggs subjected to ungrease treatment, adding protease for hydrolyzing, regulating pH value of the hydrolysate into neutral after the hydrolysis is ended, performing ultrafiltration and desalting the supernatant fluid of the centrifuged hydrolysate, concentrating and drying to obtain the fish egg sugar protein. The prepared fish egg sugar protein peptide can be used for preparing health-care food, functional food or medicines for preventing and treating osteoporosis. The used raw materials are rich and readily available, the product can perfectly keep the structure of sialic acid in the fish egg sugar protein, and the method is simple in process steps, low in equipment investment, free in environmental pollution and suitable for industrial production. The product has high anti-osteoporosis activity and can be develop the food or medicines with the effects of safely and effectively preventing and treating osteoporosis; and meanwhile, the defects of the development and utilization of fish egg sugar protein are overcome, and a novel way for high-valued processing of fish eggs is developed.

The invention provides a fish digestion-resistant peptide with an oxidation resisting function. The fish digestion-resistant peptide has the amino acid sequence shown in SEQ ID NO: 1-11. The fish digestion-resistant peptide is prepared through hydrolyzing fish protein by using an animal proteolysis compound enzyme, carrying out centrifugation, and extracting supernatant; and carrying out ultrafiltration on the supernatant by using an ultrafiltration membrane with the molecular weight cutoff of 3,000Da, collecting filtrate, and then, carrying out concentrating and freeze-drying. The fish digestion-resistant peptide with the oxidation resisting function, provided by the invention, has relatively good free-radical removing capacity and can still maintain very good oxidation resistance after the fish digestion-resistant peptide is subjected to digestion and absorption by a gastrointestinal tract; and furthermore, natural fishes serve as a raw material, so that the prepared fish digestion-resistant peptide is safe and non-toxic. According to the fish digestion-resistant peptide, the preparation method is simple, the problem in the industrialized production of the fish digestion-resistant peptide can be effectively solved, and thus the fish digestion-resistant peptide has relatively high social and economic benefits.

The invention discloses a method for preparing fish sauce through rapid fermentation of gonads of sea cucumbers. The method comprises steps as follows: (1), the gonads of the sea cucumbers are mashed to form paste, 100-120 U/g of protease is added, and the mixture is hydrolyzed for 3-5 days; (2), koji is inoculated for fermentation, and the mixture is fermented for 40-50 days; (3), exogenous fish viscera are added for fermentation; (4), yeast is added to a fermentation broth in the step (3), and the mixture is fermented for 40-50 days; (5), the fermentation temperature in the step (4) is reduced to 20-25 DEG C, and the mixture is fermented for 3-4 months; (6), the fermentation broth in the step (5) is sterilized and canned, and a finished product is obtained. In combination of a conventional fish sauce fermentation technology, improvement is performed; the whole fermentation process is in a low-salt state, and the stinking smell problem difficult to solve during preparation of the fish sauce is solved. According to the method, the fermentation time is short, and the prepared fish sauce tastes unique and has rich nutrition and certain health-care functions.

The method for extracting tetrodotoxin from liver of globefish includes the following steps: cutting liver of globefish, soaking at low temp., stirring, filtering, freezing, repeatedly soaking for several times to obtain crude venom, freezing and standing still, precipitating, heating and boiling, discharging out venom, freezing, and separating out supernatant venom, regulating pH value, adding active carbon, stirring, filtering, mixing cation 110 resin and said clear liquor, stirring, filtering, making said clear liquor pass through cation 110 resin, saturating and using acid to make elution, collecting eluant, regulating pH value, cooling, standing still, crystallizing, sucking out mother liquor, filtering, more regulating pH, freezing, standing still, crystallizing and repeatedly regulating pH for several times to obtain white powder crystal.

The invention discloses a fish protein fertilizer capable of promoting the growth of plants and a preparation method of the fish protein fertilizer. The fish protein fertilizer comprises the followingcomponents in parts by weight: 65-85 parts of fish proteins, 4-6 parts of methionine, 3-5 parts of tryptophan and 15-20 parts of modified chitosan. The preparation method of the fish protein fertilizer comprises the steps: firstly, adding methionine and tryptophan into the fish proteins so as to be premixed, and carrying out grinding and sieving to obtain protein powder with uniform particle size; secondly, adding the protein powder into a ball mill, adding water serving as a medium water and modified chitosan, and adsorbing the protein powder into the modified chitosan by mechanical mixing to obtain a wet product of the fish protein fertilizer; and thirdly, placing the wet product of the fish protein fertilizer into a vacuum drying oven, and carrying out drying to obtain a finished product of the fish protein fertilizer. The prepared fish protein fertilizer is free of salts and heavy metal residues, high in nitrogen content, good in water solubility and long in shelf life, can be widely applied to operation of fertilizer application to leaves and root systems of plants and plays a role in actively promoting the growth of crops.

The invention provides a preparation method of a fish protein peptide powder. The preparation method comprises the following steps: adding protease into fish meat paste so as to carry out enzymolysis, performing enzyme denaturalization after enzymolysis, further performing centrifugation and microfiltration by using a ceramic film so as to obtain a fish protein peptide liquid, and drying the fish protein peptide, thereby obtaining the fish protein peptide powder, wherein the protease is selected from one or more of neutral protease, papain and pancreatin; and the addition amount of the protease is 400-1500 IU/g. The preparation method is simple in process, easy to operate and beneficial to industrial production; and the produced fish protein peptide is administrated in a rubbing manner, and has a function of promoting heal of scalded skin when the dosage is 50-150mg for each wound surface.

The invention relates to a method for producing a carbon adsorption material with fish scales (carps). The method is as follows: (1) after being aired, fish scales which are taken as a raw material (not required to be ground) are uniformly mixed with a certain activator (KOH or H<3>PO<4> or ZnCl<2>) (the weight ratio of the activator to the fish scales is (1.5 to 3):1) and steeped for 10 to 24 hours; (2) after the steeped material is activated under the high temperature of 500 DEG C to 850 DEG C (potassium hydroxide is within a range from 800 DEG C to 850 DEG C; and phosphoric acid or zinc chloride is within a range from 500 DEG C to 600 DEG C, not under inert gas shielding ) for 30 to 90 minutes, an activated fish scale material is prepared; (3) the obtained activated scale material is washed with water (the activator is recovered) to be almost neutral (pH value is 6 to 8), and after drying, the finished scale carbon adsorption material is prepared; (4) the specific surface area of the prepared fish scale carbon adsorption material reaches 530m<2>/g to 992m<2>/g, and the methylene blue adsorption value is 128mg/g to 227mg/g. According to the invention, the carbon adsorption material which is prepared with the fish scales as raw materials can replace a variety of applications of active carbon for adsorption, helps realizinge the utilization of fish scale waste as a resource, and helps save resources such as timers, coal and coconut shells.

The invention provides a preparation method of fish fin tissues for extracting genomic DNA (deoxyribonucleic acid) of fish, relating to a preparation method of animal tissues for extracting the genomic DNA (deoxyribonucleic acid). The preparation method is used for solving the problems that an existing preparation method of a fish fin tissue sample of the fish requires high preservation conditions, and is unfit for field sampling and the carrying is inconvenient. The preparation method comprises the following steps: (1) collecting the fresh fish fin tissues; (2) flatly putting the fish fin tissues on filter paper, and covering the fish fin tissues by another piece of filter paper; (3) putting the fish fin tissues covered by the filter paper at a cool and ventilation place until the sample is totally dried; (4) carrying out damp-proof preservation on the sample under the room temperature. The preparation method has the advantages that the prepared fish fin tissue sample can be preserved for a long time under the normal temperature and does not need a low-temperature environment, so that the occupied space is reduced, and the preservation cost is lowered; the operation process is concise, the sample is convenient to carry, the workload can be reduced particularly when the quantity of the samples is relative large, the working efficiency is increased, and meanwhile, the experimental quality is guaranteed.

The invention relates to an ethyl ester type fish oil refining processing technology, which specifically comprises: S1, pre-treating crude fish oil, wherein an adsorbent is added to crude fish oil, the crude fish oil is stirred and adsorbed by a thermomagnetic stirrer, thermal insulation stirring is performed for 30-40 min, the fish oil is cooled to a room temperature, the cooled fish oil is filtered to remove the adsorbent, and the obtained fish oil is used for spare; S2, carrying out a pre-esterification reaction, wherein the prepared fish oil is pumped into a reaction kettle, a uniformly-mixed mixture of an acid catalyst and ethanol is added to the reaction kettle, condensation water is introduced while stirring is performed, and the acid catalyst can catalyze the pre-esterification reaction of the fish oil under an ethanol reflux state to reduce the acid value of the fish oil; S3, carrying out an esterification reaction, wherein the fish oil obtained by the pre-esterification reaction in the step S2 is pumped into a reaction distillation tower, and an esterification reaction for fish oil refining and distillation concentration are synchronously performed in the reaction distillation tower, and the esterification reaction is an enzyme and alkali combined catalytic reaction; and S4, carrying out freeze decolorization and deoxidation on the fish oil prepared by the esterification reaction in the step S3 to obtain the ethyl ester type fish oil.

The invention relates to a preparation method of a fish bait for resisting marine pathogenic vibrios. The preparation method comprises the following steps: heating and steaming glutinous rice, adding ethanol with a mass concentration of 30%-70% while the glutinous rice is hot, and controlling a mass volume ratio of the glutinous rice to the ethanol to be (1:0.01)-(1:0.05) g/mL; uniformly mixing and cooling, sequentially adding rape seed cake powder and a fructus corni extracting solution in proportion, and uniformly stirring, chopping and drying to obtain the fish bait; wherein a mass ratio of the glutinous rice to the rape seed cake powder is 1:1 to 1:10, and a mass volume ratio of the glutinous rice to the fructus corni extracting solution is 1:0.1 to 1:10 g/mL. The fructus corni is taken as a raw material, a technology for extracting a fructus corni phenolic acid compound is optimized by adopting a Box-Behnken response surface method, the yield of the fructus corni phenolic acid compound is improved, and an activity of the extract in resisting marine pathogenic bacteria is improved. The prepared fish bait is environmentally friendly, safe, reliable and capable of effectively resisting marine pathogenic bacteria, and has high industrialization value.