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Production method of fish collagen peptide chelated calcium

A technology of fish collagen peptide and production method, applied in the production of fish collagen peptide chelated calcium from fish scales, using fish bones and fish skin, can solve problems such as environmental pollution and waste of resources

Inactive Publication Date: 2016-05-11
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, these fish processing wastes at home and abroad are mainly used to produce low value-added products such as fish meal and fish bone meal, and even some fish scales are directly discarded as waste, which not only wastes resources, but also causes serious damage to the environment. Pollution

Method used

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  • Production method of fish collagen peptide chelated calcium
  • Production method of fish collagen peptide chelated calcium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Add 5 L of 0.4% Ca(OH) containing 0.75% sodium percarbonate to 1 kg of tilapia fish bones 2 Solution, soaked at 4°C for 48h, rinsed with tap water, drained properly, added 1L of pure water (the final volume of the sample was about 2.0L), heated to 100°C for 60min, cooled to 50°C, and washed with 1% Ca(OH) 2 After adjusting the pH of the fish scale aqueous solution to 9.5, add 5g of alkaline protease, enzymolyze at 40°C for 4 hours, heat at 100°C for 5min to inactivate the enzyme, cool to 50°C, and adjust the pH of the enzymolysis solution with 1% food-grade phosphoric acid to 4.0, add 10g calcium chloride, stir, every 30min during the stirring process, use 0.1%Ca(OH) 2 Solution Increase the pH of the proteolysis solution to 4.5, 5.0, 5.5, and 6.0 in sequence, and make the calcium chelate at these pHs for 30 minutes, and finally adjust the pH of the solution to 8.5, add 18g of activated carbon and stir for 30 minutes. Centrifuge removes gac, then utilizes the ceramic m...

Embodiment 2

[0024] Add 2L of 0.1% Ca(OH) containing 0.5% sodium hypochlorite to 1kg large yellow croaker scales 2 Soak the solution at 4°C for 24 hours, rinse it with tap water, drain properly, add 1L of pure water (the final volume of the sample is about 1.8L), heat up to 100°C for 45min, cool to 45°C, and use 1% Ca(OH) 2 After adjusting the pH of the fish scale aqueous solution to 9.0, add 0.3g of collagenase, enzymolyze at 45°C for 5 hours, heat at 90°C for 15min to inactivate the enzyme, cool to 45°C, and adjust the pH of the enzymolysis solution to 1% food grade phosphoric acid. 4.0, add 13.5g calcium chloride, stir, every 30min during stirring, use 0.1%Ca(OH) 2 Solution Increase the pH of the proteolysis solution to 4.5, 5.0, 5.5, and 6.0 in sequence, and make the calcium chelate at these pHs for 30 minutes, and finally adjust the pH of the solution to 8.0, add 20g of activated carbon and stir for 30 minutes, and use a disc Centrifuge removes gac, then utilizes the ceramic membran...

Embodiment 3

[0026] Add 4L of 0.3% Ca(OH) containing 1.0% potassium hypochlorite to 1kg silver carp skin 2 Soak the solution at 4°C for 36 hours, rinse it with tap water, drain it properly, add 1L of pure water (the final volume of the sample is about 2.5L), heat up to 100°C for 45min, cool to 40°C, and use 1% Ca(OH) 2 After adjusting the pH of the fish scale aqueous solution to 8.5, add 0.1g trypsin, enzymolyze at 40°C for 6h, heat at 80°C for 30min to inactivate the enzyme, cool to 40°C, and adjust the pH of the enzymolysis solution to 4.0, add 25g calcium chloride, stir, every 30min during the stirring process, use 0.1%Ca(OH) 2Solution Increase the pH of the proteolysis solution to 4.5, 5.0, 5.5, and 6.0 in turn, and make the calcium chelate at these pHs for 30 minutes, and finally adjust the pH of the solution to 7.5, add 25g of activated carbon and stir for 30 minutes. Centrifuge removes gac, then utilizes the ceramic membrane filtration that pore size is 50nm, desalination by nanof...

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Abstract

The invention discloses a production method of fish collagen peptide chelated calcium. The production method comprises the following steps: carrying out pretreatment and proteolysis on fish bone, fish skin or fish scale which serves as raw materials, then carrying out chelation reaction on the raw materials and calcium under the gradient acidic pH condition, centrifuging and carrying out nanofiltration to remove the non-chelated calcium under the alkaline condition to obtain the fish collagen peptide chelated calcium with high content of calcium. The production method of the fish collagen peptide chelated calcium is simple and convenient in extraction process and low in cost, and is applicable to industrial production; the prepared fish collagen peptide chelated calcium has a good function of promoting absorption of the calcium.

Description

technical field [0001] The invention relates to the field of preparation of fish collagen peptide chelated calcium, in particular to a method for producing fish collagen peptide chelated calcium by using fish bones, fish skin or fish scales. Background technique [0002] Calcium in the human body mainly exists in bones and teeth, and a small part is distributed in soft tissue, extracellular fluid and blood in a free or combined state, and maintains a dynamic balance with calcium in bones and teeth. Calcium is the fifth largest element in the human body and an essential nutrient for the human body. It is mainly supplemented through dietary intake. However, calcium deficiency is widespread in both developed and developing countries. Since 90% of the calcium ingested by the human body is absorbed in the small intestine, the alkaline condition of the intestinal tract will cause calcium ions to form insoluble calcium salts and precipitate, resulting in the ingested calcium not b...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/34
CPCC12P21/06C07K14/78
Inventor 翁武银陈俊
Owner JIMEI UNIV
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