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Method for extracting fish collagen from fish skin

A fish collagen and collagen technology, which is applied in the field of fish collagen preparation, can solve the problems such as no treatment, and achieve the effects of simple operation, high safety and good product quality.

Active Publication Date: 2011-11-16
青岛科海健堂生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of them use organic or inorganic solvents in the extraction and purification process, and do not deal with heavy metals such as arsenic that may exceed the standard in the product, so they cannot meet people's needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Put the fish skin of real cod into water and soak for 5 hours, wash it, and then clean it with purified water with a conductivity of 4.3us / cm for 9 hours. The consumption of the purified water is 10 times the weight of the fish skin, and then rinse The clean fish skin is minced to obtain the cleaned and minced fish skin;

[0023] Add the minced fish skin into purified water according to the weight ratio of 1:0.9, heat it to 100°C, and keep it warm for 30 minutes to obtain the sterilized fish skin;

[0024] Add protease to the above fish skin at 50°C for enzymolysis for 0.5 hours, then heat up to 120°C to inactivate the enzyme for 2 minutes to obtain crude collagen extract;

[0025] The protease is a mixture of neutral protease derived from Bacillus subtilis and bromelain derived from pineapple. The neutral protease adopts the enzyme produced by Nanning Pangbo Biological Engineering Co., Ltd., and the bromelain adopts Nanning Pangbo The brand name of Bioengineering Co.,...

Embodiment 2

[0037] Soak the pollock skin in water for 3 hours, wash it, and then wash it with purified water with a conductivity of 3 us / cm for 5 hours. The amount of purified water is 5 times the weight of the fish skin, and then rinse it The fish skin is minced to obtain cleaned and minced fish skin;

[0038] Add the minced fish skin into purified water at a weight ratio of 1:0.3 to water, heat to 95° C., and keep it warm for 10 minutes to obtain sterilized fish skin;

[0039] Add protease to the above fish skin at 20°C for enzymolysis for 1 hour, then raise the temperature to 110°C to inactivate the enzyme for 8 minutes to obtain a crude collagen extract;

[0040] The protease is a mixture of neutral protease derived from Bacillus subtilis and bromelain derived from pineapple. The neutral protease adopts the enzyme produced by Nanning Pangbo Biological Engineering Co., Ltd., and the bromelain adopts Nanning Pangbo The trademark of Bioengineering Co., Ltd. is the enzyme bromelain, and ...

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Abstract

The invention provides a method for extracting fish collagen from fish skin. The method comprises the following steps: (1) extracting collagen: adding protease to the fish skin to carry out enzymolysis, then killing the protease to obtain a collagen crude extracting solution; (2) decolorizing: adding pure active carbon to the prepared collagen crude extracting solution from the step (1) while carrying out stirring, then carrying out decolorizing, deodorizing and filtering to obtain a collagen clear solution; (3) removing a heavy metal: carrying out absorbing for the resulting collagen clear solution from the step (2) through a resin for removing arsenic, then washing the resin for removing the arsenic through purified water and collecting eluent; (4) finishing the product: carrying out evaporating, condensing, spray drying for the resulting eluent from the step (3) to obtain the fish collagen having a molecular weight of 2000-3000 dalton. With the present invention, the process for the fish collagen is simple; product quality of the fish collagen is good; toxic and harmful reagents are not remained in the prepared fish collagen product; the heavy metal in deep sea fishes can be removed well so as to effectively control the product quality and provide high safety.

Description

technical field [0001] The invention relates to a preparation method of fish collagen. Background technique [0002] At present, there are many studies on extracting collagen from fish skin, and the general process includes the steps of raw material treatment, enzymatic hydrolysis, filtration, concentration and spray drying. However, most of them use organic or inorganic solvents in the extraction and purification process, and do not deal with heavy metals such as arsenic that may exceed the standard in the product, so they cannot meet people's needs. Contents of the invention [0003] The purpose of the present invention is to provide a method for extracting fish collagen from fish skin, so as to overcome the above-mentioned defects in the prior art. [0004] The method of the present invention comprises the steps of: [0005] (1) Collagen extraction: put fish skin at 20-50°C, add protease for enzymolysis, the enzymolysis time is 0.5-1 hour, then raise the temperature t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02
Inventor 郑元生
Owner 青岛科海健堂生物有限公司
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