152 results about "Collagen product" patented technology

The invention discloses a method for preparing collagen oligopeptide. Animal skins are taken as raw materials, and are subjected to acid and surfactant pretreatment, enzymolysis, decoloration, membrane separation and drying to form the collagen oligopeptide. The relative molecular weight of the prepared collagen oligopeptide is mainly distributed below 1,000Dalton, and preferably, the Gaussian distribution peak area percentage of oligopeptide with the relative molecular weight of less than 1,000Dalton is over 85 percent and preferably over 90 percent. The method solves the general problems that the conventional collagen products have odor, are dark yellow and the like; and the obtained collagen oligopeptide powder is white, free of odor and good in mouthfeel, has high absorptivity and penetrability and can be widely applied to the cosmetics industry, the food industry and the like.

The invention relates to recombinant human-derived collagen series products for skin barrier function injuries or haemorrhoids, in particular to a medical recombinant human-derived collagen function dressing (spray type), medical skin collagen repair function dressing, medical recombinant human-derived collagen function dressing (gel type) and a collagen trauma hemostatic material (hemorrhoids type). The product provided by the invention has an obvious curative effect on skin barrier function injuries or haemorrhoids, and has popularization and application value.

The present invention addresses the treatment of ocular conditions by the enhancement of lens regeneration. This is accomplished by the administration of a high viscosity composition including a hyaluronic acid compound and laser photoablation. Excess high viscosity composition may be removed by focal laser photophacoablation. Additionally, a collagen product may be injected within the lens capsule to improve lens cell proliferation and differentiation, and to improve the configuration, shape and structure of regenerated lenses. Various embodiments involving the enhancement of lens regeneration are described. For example, lens regeneration may be enhanced by filling the lens capsule bag with the inventive hyaluronic acid compound; by inserting at least one collagen patch in the lens capsule; and / or by injecting a collagen-based product into the lens capsule.

The invention discloses a method for preparing low-molecular-weight aquatic collagen peptides. Fresh fish scales are delimed with hydrochloric acid, fat and impurities are removed with caustic soda, and then hot water extraction is carried out to obtain a collagen solution, and three kinds of enzymes are used to separate enzyme Decompose collagen, then carry out activated carbon filtration and macroporous resin treatment, collect decolorization liquid, filter through ultrafiltration membrane, freeze-dry, the effective method of preparation molecular weight concentration, high recovery rate, good decolorization effect fish scale low molecular weight collagen, not only can Obtain high-content low-molecular-weight collagen peptides from fish skin and fish scales, remove pigments that affect the appearance of collagen products, and reduce the unique fishy smell of collagen, which can improve the product value of fish collagen products.

The invention discloses a method of extracting non-denaturing natural collagen from the animal skin or / and the tendon, and the method comprises the following steps the raw material chips of the animal skin or / and the tendon in which the lipa and the impurity are removed, and which is soaked and per-processed through acetone is added into a reactor and soaked with acetate solution, stomach cnzyme is added after full soaking, the enzymolysis is performed under the irradiation of the ultrasonic wave, after enzymolysis, enzymolysis liquid is diluted with the acetate solution, the part that is not completely enzyme-decomposed is separated and removed in the enzymolysis liquid, obtained collagen aqueous solution is salted out with 2 mol / L NaCl or (NH4)2SO4 solution, collagen salted object obtained by separating and salting out suspended material is stirred and dialyzed with distilled water, and the product after being dialyzed is frozen out through the freezing dryer, namely, the collogen product is obtained. By adopting the method of the invention to prepare the collogen, the extraction time of the collogen can be greatly saved, the collogen production rate is improved, and the obtained collogen can completely retain the three unique spiral structures, which can be applied to the biological medicine material.

In various embodiments, a collagen product is provided that is derived from an animal, the collagen product comprises precipitated collagen that is substantially pure. In various embodiments, the collagen is obtained from a marine animal and does not contain prions or viruses. In various embodiments, the collagen can be made or incorporated into collagen films, collagen membranes, cosmetic collagen masks, collagen sponges, gelatin, hemostasis sponges, lyophilized foams, collagen injections, artificial skins and dura, bones, cartilage, screws, shafts, stems, or tube guides.

The invention relates to a method for extracting acid-soluble collagen from fish skin and an acid-soluble collagen product extracted by the method. The method comprises the following steps: removing impure protein and fat of the fish skin; extracting the acid-soluble collagen, dialyzing, performing freeze-drying and the like. The acid-soluble collagen product extracted by the method keeps an integral structure and is not degradable; and the acid-soluble collagen product has a denaturation temperature similar to that of collagen from a large terrestrial animal, can be used as a substitute for the collagen from the large terrestrial animal, and has a potential application value.

Methods of sterilizing dermal fillers and injectable collagen material have been developed which reduce the level of active biological contaminants or pathogens without adversely affecting the material, i.e., wherein the dermal fillers and injectable collagen material retain their same properties before and after its terminal sterilization. In one embodiment the method for sterilizing the dermal filler or injectable collagen material that is sensitive to radiation contains the steps of protecting the filler or material from radiation, and irradiating the filler or material with a suitable dose of radiation for a time and at a rate effective to sterilize the filler or injectable material. In a preferred embodiment the method for sterilizing the dermal filler or injectable collagen material that is sensitive to radiation includes the steps of a) freezing the filler or material at a temperature below its freezing temperature, which is generally below 0° C. and b) irradiating the filler or material with a suitable dose of radiation at an effective rate for a time effective to sterilize the filler or material. The exposure of the radiation differs depending upon the density of the filler or material, but is preferably between 5kGy and 12kGy and more preferably between 6kGy and 8kGy. These doses result in a sterility assurance level (SAL) of 10−6 SAL for the filler or material.

The invention relates to a collagenolysis and regeneration method, aiming at providing a simple and convenient collagenolysis method; meanwhile, the invention further provides a method which leads the lysis collagen to regenerate membranes, fibers, microballoons or powdery products. In order to realize the purpose, the invention adopts the following technical proposal: in the collagenolysis method, ionic liquid is utilized as solvent for collagen lysis, and the ionic liquid is alkyl imidazoles, including 1-methyl-3-butyl halogenated imidazole, 1-butyl-3-methyl halogenated imidazole, 1-ethyl-3-methyl halogenated imidazole and 1-allyl-3-methyl halogenated imidazole or halide (3,3-dimethyl) diimidazole sulfoxide salt. In the collagen regeneration method, the collagenolysis method is utilized to prepare collagen / ionic liquid solution, and then coagulators are utilized to wash out the ionic liquid in the solution, and then the regeneration collagen is obtained. In the collagenolysis and regeneration method, the steps are simple, nontoxic and harmless, and have high safety, thereby establishing good basis for research and application and dissemination of the collagen products.

The invention discloses a method for extracting macromolecular medical collagens from fish skins, and an application of the fish skin collagens as a tissue restoration scaffold material. The method comprises the following steps: 1, preprocessing fish skins; 2, cleaning the fish skins through various technologies; 3, extracting the collagens through an acid technology; 4, processing the collagens through an immobilized enzyme technology; and 5, purifying the collagens. The collagen preparation method has the advantages of low energy consumption, short period, simple operation, and suitableness for industrial large-scale production, and the prepared collagens have the advantages of high purity, low immunogenicity and good biocompatibility, and are suitable for being processed to develop medical collagen products. The invention also discloses an application of the fish skin collagens in the production of a scaffold material and an application of the scaffold material in wound restoration.

The invention discloses an extracting method for a sturgeon skin collagen. The extracting method comprises the following steps: (1) preliminary treatment: taking and slicing a sturgeon skin, firstly immersing the sturgeon skin in alkaline solution, immersing the sturgeon skin in an acidic solution after rinsing, and then taking the sturgeon skin out for rinsing and standby; (2) extraction: adding water into the preprocessed sturgeon skin for leaching at 45 to 55 DEG C, and carrying out the filtration to remove impurities and obtain the collagen extracting solution; and (3) freeze drying: obtaining sturgeon skin collagen powder through the refrigerated centrifugation of the collagen extracting solution. The collagen product has higher quality. The extracting method has a higher collagen extraction rate, and not only improves the product yield, but also saves the cost in a simple way, thereby improving the economic benefits of a manufacturing enterprise.

The invention relates to an aquatic collagen fish ball and a preparation method thereof. The fish ball contains the following raw materials by mass: 40-70% of aquatic collagen, 1-10% of soybean protein isolate, 5-15% of fat pork, 10-30% of starch, 0.5-2% of egg white protein, 0.5-5% of a thickening agent, 5-10% of a seasoning, 0.01-0.5% of a calcium salt, 0.1-1% of a protein cross-linking agent, and the balance ice water. The preparation method of the fish ball includes: preparing the aquatic collagen into gel peptone, conducting chopping, then mixing the chopped gel peptone with the soybean protein isolate in certain proportion, carrying out pulping and emulsification, then adding auxiliary materials, the protein cross-linking agent and the seasoning, mixing the materials evenly, then performing molding, quick-freezing, storage and packaging, thus obtaining the finished fish ball product. The fish ball provided by the invention has the characteristics of good heat resistance, delicious taste, good elasticity, crisp and tender taste, rich nutrition, and convenience eating. The preparation method of the fish ball provided by the invention has the advantages of simple operation, high efficiency and low cost, effectively solves the problems of poor heat resistance and lack of raw materials in traditional collagen products, and is suitable for popularization and application.

The invention provides a method for extracting fish collagen from fish skin. The method comprises the following steps: (1) extracting collagen: adding protease to the fish skin to carry out enzymolysis, then killing the protease to obtain a collagen crude extracting solution; (2) decolorizing: adding pure active carbon to the prepared collagen crude extracting solution from the step (1) while carrying out stirring, then carrying out decolorizing, deodorizing and filtering to obtain a collagen clear solution; (3) removing a heavy metal: carrying out absorbing for the resulting collagen clear solution from the step (2) through a resin for removing arsenic, then washing the resin for removing the arsenic through purified water and collecting eluent; (4) finishing the product: carrying out evaporating, condensing, spray drying for the resulting eluent from the step (3) to obtain the fish collagen having a molecular weight of 2000-3000 dalton. With the present invention, the process for the fish collagen is simple; product quality of the fish collagen is good; toxic and harmful reagents are not remained in the prepared fish collagen product; the heavy metal in deep sea fishes can be removed well so as to effectively control the product quality and provide high safety.

The invention relates to a preparation technology for producing collagen and in particular relates to a preparation technology for producing collagen through enzymolysis of fish scales, belonging to the technical field of collagen extraction. The preparation technology comprises the following steps of (1) washing, (2) alkali treatment, (3) decalcification, (4) cooking, (5) cooling, (6) primary enzymolysis, (7) secondary enzymolysis, (8) yeast fermentation of a fish scale collagen solution, (9) activated carbon adsorption reaction, (10) filtration, (11) concentration, (12) sterilization and (13) spray drying. The method provided by the invention not only can achieve the effect of obtaining collagen peptides with high contents and low molecular components from the fish scales but also can achieve the effects of removing pigment substances affecting the appearances of collagen products and reducing the peculiar fishy smell of collagen, thus improving the values of fish collagen products.

The invention provides a new technology for preparing high purity fish scale collagen. The technology uses fish scale as raw material which is soaked in acid base, smashed by a colloid mill and added with protease for enzymolysis; an ion-exchange column chromatographic column separation-membrane separation combined technique is used for separating and purifying the extracted collagen, thus obtaining the collagen with larger than 95% of purity and 1000-5000Da or 100, 000-300, 000Da of molecular weight distribution; and the collagen can be used for preparing health care products, cosmetics and biomedical engineering materials. By adopting the new technology, the waste water production quantity is only 50% of the traditional technology.

Methods of sterilizing dermal fillers and injectable collagen material have been developed which reduce the level of active biological contaminants or pathogens without adversely affecting the material, i.e., wherein the dermal fillers and injectable collagen material retain their same properties before and after its terminal sterilization. In one embodiment the method for sterilizing the dermal filler or injectable collagen material that is sensitive to radiation contains the steps of protecting the filler or material from radiation, and irradiating the filler or material with a suitable dose of radiation for a time and at a rate effective to sterilize the filler or injectable material. In a preferred embodiment the method for sterilizing the dermal filler or injectable collagen material that is sensitive to radiation includes the steps of a) freezing the filler or material at a temperature below its freezing temperature, which is generally below 0° C. and b) irradiating the filler or material with a suitable dose of radiation at an effective rate for a time effective to sterilize the filler or material. The exposure of the radiation differs depending upon the density of the filler or material, but is preferably between 5 kGy and 12 kGy and more preferably between 6 kGy and 8 kGy. These doses result in a sterility assurance level (SAL) of 10−6 SAL for the filler or material.

The invention relates to a method for manufacturing collagen from black sea cucumbers from East China Sea, which comprises the following steps: (1) adding Alcalase hydrolysis protease in an amount which is 0.01 to 10 percent based on the weight of black sea cucumbers from East China Sea, protamex composite protease in an amount which is 0.01 to 10 percent based on the weight of black sea cucumbers from East China Sea and flavourzyme in an amount which is 0.01 to 10 percent based on the weight of black sea cucumbers from East China Sea into the black sea cucumbers from East China Sea which are subjected to foul-removing and cleaning treatment, heating the materials to 45 to 47 DEG C, and hydrolyzing at a constant temperature of 45 to 47 DEG C with stirring for 4 hours; and (2) after enzyme hydrolysis is finished, performing fine filtration by a membrane filter, concentrating in a vacuum concentrator, keeping the temperature of the materials in the vacuum concentrator between 55 and 58 DEG C by controlling the heating steam valve of the vacuum concentrator, and concentrating to obtain pasty or powder collagen product from black sea cucumbers from East China Sea. The method has the advantages that: a technical route of combination of three enzymes and hydrolysis degree intermediate detection is adopted, so the relative molecular weight of the product of the enzyme hydrolysis of the black sea cucumbers from East China Sea can be controlled to meet the requirement on the relative molecular weight of sea cucumber collagen products; and the product of the hydrolysis product is free from bitter taste and fishy smell.

The invention belongs to the collagen hydrolysis field, and in particular to the method for extracting low molecular weight collagen from cattle bone. According to the invention, the cattle bone after being degreased is crushed and grinded to bone meal with particle size of 0.5-1000mum; the bone meal is dispersed in water to form bone slurry, a series postprocessing technology is employed to remove enzymatic hydrolysis and enzymes inactivating is carried out to obtain solid residues and soluble inorganic salt impurity in a protein liquid, thereby the low molecular weight collagen with high purity can be obtained. According to the invention, the low molecular weight collagen product with average molecular weight of 1000-5000g / mol can be obtained, the ash content is lower than 1.5%, and the obtained low molecular weight collagen can be used in food, medicine, and cosmetic and health product.

The invention relates to a preparation method of collagen; fresh pigskin is used as a raw material; the raw material is treated by pretreatment, fat removal, and acid-enzyme treatment; ultrasonic treatment is employed to further improve extraction efficiency; and finally purification is performed to prepare a collagen product with high purity. The preparation method of collagen has high extraction efficiency, and the product purity is up to above 98%. The product is active collagen with a maintained triple-helix structure, has low immunogenicity and high biocompatibility, is biodegradable, and is applicable to medical purposes.

The invention relates to a method for preparing a high-strength collagen tissue repair material product. The method obtains the final collagen product through the treatment steps of salting out, centrifugation, dehydration, crosslinking and drying of a collagen solution. Because the treatment method adopts the mode of improving the solid content of collagen in collagen gel and the crosslinking density in the collagen gel, the mechanical property of the final product is greatly improved. Simultaneously, because the adopted method does not influence the biological property of the collagen, the final collagen product still maintains good biological activity. The collagen product can be used for the repair treatment of articular cartilage, meniscus and skin, the construction of artificial blood vessels and nerve conduits, and the like.

The invention discloses a novel method for preparing high-activity collagen peptide from cow placentae. The method comprises the following steps: maintaining mammal placentae at a low temperature, chopping, soaking and rinsing, grinding into pulp, extracting, centrifuging, performing enzymolysis, filtering, desalting, concentrating and freeze-drying. The method disclosed by the invention has the characteristics that the extraction effect is good, the high-activity collagen peptide prepared by using the method is high in purity, the activity is high, a product containing the collagen peptide is easy to absorb, and the like. The method can be simply operated, is low in production cost and is applicable to on-scale industrialization production, and the purpose that the high-activity collagen peptide is efficiently prepared from cow placentae is achieved.

The disclosed collagen extracted from jellyfish mesoglea belongs to white fiber solid or light-yellow solid powder with bulk density as 0.110-0.645g / ml, shows clear liquid after dissolving in acidic solution completely; has single-bond molecular weight as 100-300 thousands dalton, sugar content as 2.5-5%, amino acid content as 80-95%mg / 100mg with 7.9-8.3% aspartic acid, 10.0-12.9% glutamic acid, 3.5-4.0% threonine, 3.5-3.9% valine, 2.2 -2.5% isoleucine, 3.4-3.8% lycine, and 2.0-2.7% hydroxylysine. Besides, the opposite preparation method comprises: (1) selecting salinized or fresh jellyfish; (2) pre-treating; (3) separating the mesoglea; (4) dissociating mesoglea; (5) ultrafiltrating, separating, and condensing. This invention has trapping rate as 95-98% and high product purity.

This invention relates to an in-situ dechroming approach on a wet-blue leather and the leftovers. The process comprises that organic acids and organic acid salt dechromize step by step in the water solution at room temperature; firstly use the 1 per cent to 40 per cent organic acids to treat the leather, and then use the 1 per cent to 40 per cent organic acid salt to remove chromium. The invention is characterized in that the invention keeps the original structure of the collagen fibers with the dechroming rate as high as 99.6 per cent. The operation is easy and doesn't produce toxic hexavalent chromium. The material can be selected according to the goal products need. Suitable organic acids and organic acid salt can be applied for various post-process. In this way, the approach can obtain different forms of collagen products, especially the high value-added filamentous leather fibers. The approach also can be used for the tanning retire on the surface of the chrome leather with modest process condition, clean wet-blue leather grains and fine grain surfaces.

Foam-formed collagen strands and methods for forming strands involve depositing a dispersed solution of an isolated cleaned, de-fatted, enzymatically-treated (or non-enzyme treated) human-derived collagen product having a preserved amount of its natural constituents into grooves of a grooved plate, and processing the dispersed collagen product to provide a foam-formed collagen strand. Foam-formed collagen strands may be processed into threads having a matrix of reticulated pores to conduct biological materials in and through the strand, the collagen of the collagen strand comprising isolated, enzymatically-treated human derived collagen having a preserved amount of its natural collagen constituents.

The invention belongs to the field of biotechnology and discloses a method of preparing collagen by using fish skins and scales as raw materials. The method of preparing the collagen comprises the following steps: cleaning the fish skins and scales, adding a 0.01-0.1mol / L citric acid solution, carrying out ultrasonic treatment for 4-8 h, regulating the pH value to be 6.0-7.5 by adding an alkali solution, and drying and filtering; adding water in the fish skins and scales which are dried and filtered, milling into fish skin and scale slurry by a colloid mill, regulating the pH value to be 8.0-8.5 by adding the alkali solution, adding alkaline protease for enzymatic hydrolysis for 60-180 min, and carrying out inactivation on the protease to obtain a collagen solution. According to the method disclosed by the invention, the fish skins and scales are degreased and deashed by the technology of combining citric acid treatment and ultrasonic waves; meanwhile, with adoption of the technology of combining colloid mill treatment and alkaline protease enzymatic hydrolysis, enzymatic hydrolysis is rapid, and protein macromolecules are completely hydrolyzed into small molecules, thus increasing extraction rate of the collagen and enabling the prepared collagen product to be safe and natural.

A preparation method of cod skin collagen, which is characterized in that the method comprises thawing frozen cod skin sample, removing impurity, washing via tap water and breaking mechanically, adding pretreated cod skin into hydrochloride to be immerged, mixing uniformly, adjusting pH value, bathing in water and centrifugally treating, extracting the left slag via hot water, centrifugally treating, combining extracts, concentrating and drying to obtain collagen product. The invention has short production period, whose product is white or light yellow, has high quality and is soluble in water.

The invention relates to an aquatic collagen fish bean curd and a preparation method thereof. The fish bean curd is composed of the following raw materials by mass: 65-90% of aquatic collagen glue peptone, 2.5% of soybean isolate protein, 0.1-0.5% of a protein cross-linking agent, 0.5-2% of egg white protein, 1-5% of vegetable protein, 0-10% of starch, 1-8% of pork fat, 0.1-2% of thermal reversible colloid, 0.1-1% of thermal irreversible glue, and 2-10% of a seasoning. The preparation method of the fish bean curd comprises: preparing the raw materials into a collagen gel peptone shape, then adding soybean isolate protein to perform pulping and adding the protein cross-linking agent to conduct chopping, then adding auxiliary materials and the seasoning, mixing the materials evenly and carrying out rough processing shaping, finally conducting deep processing to obtain a finished product and then carrying out vacuum packaging, sterilizing, cooling, encasement and warehousing. The fish bean curd provided by the invention has the characteristics of high protein content, rich nutrition and convenient eating, and solves the technical barrier that traditional collagen products have poor heat resistance. The preparation method of the fish bean curd provided by the invention has the advantages of simple and convenient operation and high efficiency, and is suitable for popularization and application.

The present invention addresses the treatment of ocular conditions by the enhancement of lens regeneration. This is accomplished by the administration of a high viscosity composition including a hyaluronic acid compound. Excess high viscosity composition may be removed by focal laser photophacoablation. Additionally, a collagen product may be injected within the lens capsule to improve lens cell proliferation and differentiation, and to improve the configuration, shape and structure of regenerated lenses. Various embodiments involving the enhancement of lens regeneration are described. For example, lens regeneration may be enhanced by filling the lens capsule bag with the inventive hyaluronic acid compound; by inserting at least one collagen patch in the lens capsule; and / or by injecting a collagen-based product into the lens capsule.