Method for extracting acid-soluble collagen from fish skin and extracted product
A collagen, acid-soluble technology, which can be used in the preparation of peptides, chemical instruments and methods, animal/human proteins, etc., and can solve problems such as lack of telopeptides
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Embodiment 1
[0070] Embodiment 1: extract acid-soluble collagen from fish skin
[0071] Fresh grass carp is slaughtered and skinned in a cold room, and the residual fish meat on the skin is removed. Then, wash the fish skin with ice water, cut into small pieces and store in the refrigerator for later use.
[0072] The implementation steps are as follows:
[0073] Step A: Remove fish skin impurities
[0074] According to the ratio of fish skin in grams to aqueous sodium hydroxide solution in milliliters: 1:20, the fish skin was treated with 0.05M aqueous sodium hydroxide solution at a temperature of 4° C. for 35 hours to obtain fish skin from which impurities were removed.
[0075] Step B: Remove the skin fat from the fish
[0076] According to the ratio of protein fish skin in grams to fat-removing solution in milliliters: 1:10, the fish skin obtained in step A) was then treated with n-hexane fat-removing solution at a temperature of 4°C for 20 hours to obtain fat-removing fish Skin. ...
Embodiment 2
[0088] Embodiment 2: extract acid-soluble collagen from fish skin
[0089] Fresh carp are slaughtered and skinned in a cold room, and the residual fish meat on the skin is removed. Then, wash the fish skin with ice water, cut into small pieces and store in the refrigerator for later use.
[0090] The implementation steps are as follows:
[0091] Step A: Remove fish skin impurities
[0092] According to the ratio of fish skin in grams to aqueous sodium hydroxide solution in milliliters: 4:35, the fish skin was treated with 0.1 M sodium hydroxide aqueous solution at a temperature of 10° C. for 32 hours to obtain fish skin from which impurities were removed.
[0093] Step B: Remove the skin fat from the fish
[0094]According to the ratio of protein fish skin in grams to degreasing solution in milliliters: 4:16, the fish skin obtained in step A) was then treated with absolute ethanol degreasing solution at a temperature of 10°C for 26 hours to obtain fat-removing fish skin. ...
Embodiment 3
[0106] Embodiment 3: extract acid-soluble collagen from fish skin
[0107] Fresh grass carp is slaughtered and skinned in a cold room, and the residual fish meat on the skin is removed. Then, wash the fish skin with ice water, cut into small pieces and store in the refrigerator for later use.
[0108] The implementation steps are as follows:
[0109] Step A: Remove fish skin impurities
[0110] According to the ratio of fish skin in grams to sodium hydroxide aqueous solution in milliliters: 2:32, the fish skin was treated with 0.2M sodium hydroxide aqueous solution at a temperature of 15° C. for 38 hours to obtain fish skin from which impurities were removed.
[0111] Step B: Remove the skin fat from the fish
[0112] According to the ratio of 2:12 for fish skin with impurities removed in grams and fat-removing solution in milliliters, the fish skin obtained in step A) was then treated with anhydrous ether fat-removing solution at a temperature of 15°C for 24 hours to obtai...
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