The invention discloses a method for aqueous two-phase extraction, separation and purification of
dendrobium officinale
polysaccharide. The invention aims to provide the method for aqueous two-phase extraction, separation and purification of the
dendrobium officinale
polysaccharide. The method has the advantages that an extraction rate, a deproteinization rate and purity are greatly improved. According to a technical scheme in the invention, the method comprises the following steps:
drying and crushing fresh stems of
dendrobium officinale; dissolving
dendrobium officinale powder in water according to a material-liquid
mass-volume ratio of 1g: (15-30) ml, conducting extracting twice at 50-60 DEG C, wherein extracting lasts for 1-3 hours each time, combining the two extracts, carrying out filtering, performing centrifuging at 6000-8000 r / min for 10-15 minutes, taking a supernatant, and concentrating the supernatant to 20-40 ml; adding 95%
ethanol, carrying out uniform shaking, performing refrigerating at 4 DEG C for 8-12 hours, carrying out centrifuging, collecting a precipitate, freeze-
drying the precipitate for 2 days,
grinding the precipitate to obtain white crude
polysaccharide powder, and dissolving the white crude polysaccharide
powder with hot water to obtain a crude polysaccharide solution with a concentration of 0.5-5mg / mL; and adding an
ethanol-
ammonium sulfate mixed solution, conducting standing for layering, carrying out extracting at 15-55 DEG C, retaining a lower-phase substance, conducting concentrating at 60 DEG C under reduced pressure, adding
ethanol, carrying out standing overnight, then performing centrifuging, collecting a precipitate, and freeze-
drying the precipitate to obtain the pure
dendrobium officinale polysaccharide.