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Method for extracting and purifying lentinan

A purification method, the technology of lentinan, applied in the field of polysaccharide extraction, can solve the problems of complicated process, high production cost, low purity, etc., and achieve the effect of safe extraction process, reduced usage, and less impurity content

Inactive Publication Date: 2015-03-25
山东迅达康兽药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the embodiment of the present invention is to provide a preparation method of high-content lentinan, which aims to solve the problems of complex process, high production cost, low purity, low polysaccharide content and low yield in the current extraction method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for extracting lentinan, which is extracted according to the following method: take mushrooms and dry them in a drying oven at 50°C, crush the dried mushrooms into 40 meshes, put the mushroom powder into water whose mass-volume ratio is 40 times, soak in a water bath at 90°C After extracting for 100min, filter to remove impurities, slowly add 0.5% chitosan solution for flocculation, evaporate and concentrate the extract to 1 / 2 of the original volume, centrifuge at 3000r / min for 15min, take the supernatant and add ethanol, the amount of ethanol added is 3 times the rear volume. Stand at 4°C for 24 hours for alcohol analysis, filter, remove the supernatant, and dry to obtain a polysaccharide product. The yield of polysaccharide is 5.3%, and the light transmittance of the extract is 89%, indicating that there are few impurities such as suspended particles, fine colloid particles and macromolecular proteins in the extract.

Embodiment 2

[0023] The difference from Example 1 is that, according to the crushing of dried shiitake mushrooms to 60 mesh, the mass volume ratio of dried shiitake mushrooms to water in a water bath is 1:60 (g:ml), and the dried shiitake mushrooms are put into a leaching temperature of 85°C. After heating in the water bath for 150 minutes, filter to remove impurities, slowly add 1% chitosan solution for flocculation, evaporate and concentrate the extract to 1 / 3 of the original volume, centrifuge at 4000r / min for 10 minutes, take the supernatant and add ethanol, The amount of ethanol added is 4 times the volume after concentration. Stand at 6°C for 24 hours for alcohol analysis, filter, remove the supernatant, and dry to obtain a polysaccharide product. The polysaccharide yield is 4.8%, and the light transmittance of the extract is 93%, indicating that there are few impurities such as suspended particles, fine colloid particles and macromolecular proteins in the extract.

Embodiment 3

[0025] The difference from Example 1 is that according to the crushing of dried shiitake mushrooms to 80 meshes, the mass volume ratio of dried shiitake mushrooms to water in a water bath is 1:80 (g:ml), and the dried shiitake mushrooms are put into a leaching temperature of 95°C. After heating in the water bath for 120 minutes, filter to remove impurities, slowly add 0.75% chitosan solution for flocculation, evaporate and concentrate the extract to 1 / 4 of the original volume, centrifuge at 5000r / min for 20 minutes, take the supernatant and add ethanol, The amount of ethanol added is twice the volume after concentration. Stand at 4°C for 24 hours for alcohol analysis, filter, remove the supernatant, and dry to obtain a polysaccharide product. The yield of polysaccharide is 5.1%, and the light transmittance of the extract is 91%, indicating that there are few impurities such as suspended particles, fine colloid particles and macromolecular proteins in the extract.

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Abstract

The invention belongs to the technical field of extraction of polysaccharides, and particularly relates to a method for extracting and purifying lentinan. The method comprises the following steps: drying mushrooms at 40-80 DEG C, and crushing the dry mushrooms into 20-100-mesh powder; heating the mushroom powder in a water bath kettle of which the leaching temperature is 60-100 DEG C for 60-150 minutes, and then filtering to remove impurities; slowly adding 0.5-5% of chitosan liquid, flocculating, filtering, evaporating and concentrating the filtrate to be 1 / 2 to 1 / 4 of original volume; centrifuging, taking supernate, adding ethanol, carrying out alcohol-precipitation and standing for 24 hours, filtering, removing the supernate, and drying, so as to obtain a lentinan product. The method is improved on the basis of a hot water extraction method; the extraction rate is improved; compared with the prior art, the method is simple in technological process, easy to operate, relatively low in cost, and low in impurity content; and large-scale production can be achieved.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide extraction, and in particular relates to an extraction method for increasing the extraction rate of lentinan. technical background [0002] Lentinus edodes is the second largest edible fungus in the world, belonging to the fungi of the Basidiomycetes Agaritaceae. The main components of shiitake mushrooms are protein, lentinan, lentinan purine, nucleotides, various minerals and dietary fiber. Lentinan is an important active factor of Lentinus edodes. It is an immune regulator without any side effects. It can prevent carcinogenic effects caused by chemical factors, inhibit tumor metastasis, and enhance the body's resistance to different bacteria, parasites and germs. [0003] The traditional extraction method of lentinan is water extraction and alcohol precipitation method, the extraction rate is low, and there are many impurities such as suspended particles, fine colloidal particles and mac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 汪安国张玉宝王尚明刘文利王玉堃杨蕾林明明谷颖
Owner 山东迅达康兽药有限公司
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