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Method for aqueous two-phase extraction, separation and purification of dendrobium officinale polysaccharide

A technology for the separation and purification of Dendrobium officinale polysaccharides, which is applied in the field of extraction, can solve the problems of multiple repetitions, high loss rate of polysaccharides, complicated operation, etc., and achieve the effects of improved purity, safe extraction, and improved polysaccharide extraction efficiency

Inactive Publication Date: 2021-05-11
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most methods for water extraction and deproteinization of dendrobium polysaccharides are protein denaturation and enzymatic hydrolysis, but these methods have disadvantages such as complicated operation, many repetitions, low deproteinization rate, and high loss rate of polysaccharides.

Method used

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  • Method for aqueous two-phase extraction, separation and purification of dendrobium officinale polysaccharide
  • Method for aqueous two-phase extraction, separation and purification of dendrobium officinale polysaccharide
  • Method for aqueous two-phase extraction, separation and purification of dendrobium officinale polysaccharide

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Embodiment 1

[0032] (1) A method for separating and purifying the polysaccharides of Dendrobium officinale by two-phase extraction, drying the fresh stems of Dendrobium officinale at 50°C, and pulverizing them to 200 mesh with a traditional Chinese medicine pulverizer; accurately weighing 10.0g of Dendrobium officinale powder, according to 1:25 feed solution Add 250mL of deionized water to the mass volume ratio, stir well; keep the solution temperature at 55-60°C, extract twice, each time for 1h; combine the two extracts, filter with gauze, centrifuge at 7500r / min for 10min, and take the supernatant The clarified extract was obtained, concentrated to 30mL with a rotary evaporator at 60°C, then added 120mL of 95% ethanol, shaken well, refrigerated at 4°C for 12h, taken out and centrifuged at 5000r / min for 15min, discarded the supernatant and left the precipitate, and the precipitate Freeze-drying was carried out for 2 days, and the white solid was ground to obtain a crude polysaccharide powd...

Embodiment 2

[0036] (1) A method for separating and purifying the polysaccharides of Dendrobium officinale by two-phase extraction, drying the fresh stems of Dendrobium officinale at 50°C, and pulverizing them to 200 mesh with a traditional Chinese medicine pulverizer; accurately weighing 10.0g of Dendrobium officinale powder, and mixing it according to the ratio of 1:20 feed solution Add 250mL of deionized water and stir evenly; keep the temperature of the solution at 60°C, extract twice, 1h each time; combine the two extracts, filter with gauze, centrifuge at 7500r / min for 10min, and take the supernatant to obtain clarification Concentrate the extract to 30mL with a rotary evaporator at 60°C, then add 120mL of 95% ethanol, shake well, refrigerate at 4°C for 12h, take it out and centrifuge at 5000r / min for 15min, discard the supernatant to save the precipitate, and freeze the precipitate After drying for 2 days, the white solid was ground to obtain a crude polysaccharide powder. The crude...

Embodiment 3

[0040] (1) A method for separating and purifying the polysaccharides of Dendrobium officinale by two-phase extraction, drying the fresh stems of Dendrobium officinale at 50°C, and pulverizing them to 200 mesh with a traditional Chinese medicine pulverizer; accurately weighing 10.0 g of Dendrobium officinale powder, and feeding it according to the ratio of 1:30 feed solution Add 250mL of deionized water and stir evenly; keep the solution temperature at 55°C, extract twice, 1h each time; combine the two extracts, filter with gauze, centrifuge at 7500r / min for 10min, and take the supernatant to obtain clarification Concentrate the extract to 30mL with a rotary evaporator at 60°C, then add 120mL of 95% ethanol, shake well, refrigerate at 4°C for 12h, take it out and centrifuge at 5000r / min for 15min, discard the supernatant to save the precipitate, and freeze the precipitate After drying for 2 days, the white solid was ground to obtain a crude polysaccharide powder. The crude poly...

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Abstract

The invention discloses a method for aqueous two-phase extraction, separation and purification of dendrobium officinale polysaccharide. The invention aims to provide the method for aqueous two-phase extraction, separation and purification of the dendrobium officinale polysaccharide. The method has the advantages that an extraction rate, a deproteinization rate and purity are greatly improved. According to a technical scheme in the invention, the method comprises the following steps: drying and crushing fresh stems of dendrobium officinale; dissolving dendrobium officinale powder in water according to a material-liquid mass-volume ratio of 1g: (15-30) ml, conducting extracting twice at 50-60 DEG C, wherein extracting lasts for 1-3 hours each time, combining the two extracts, carrying out filtering, performing centrifuging at 6000-8000 r / min for 10-15 minutes, taking a supernatant, and concentrating the supernatant to 20-40 ml; adding 95% ethanol, carrying out uniform shaking, performing refrigerating at 4 DEG C for 8-12 hours, carrying out centrifuging, collecting a precipitate, freeze-drying the precipitate for 2 days, grinding the precipitate to obtain white crude polysaccharide powder, and dissolving the white crude polysaccharide powder with hot water to obtain a crude polysaccharide solution with a concentration of 0.5-5mg / mL; and adding an ethanol-ammonium sulfate mixed solution, conducting standing for layering, carrying out extracting at 15-55 DEG C, retaining a lower-phase substance, conducting concentrating at 60 DEG C under reduced pressure, adding ethanol, carrying out standing overnight, then performing centrifuging, collecting a precipitate, and freeze-drying the precipitate to obtain the pure dendrobium officinale polysaccharide.

Description

technical field [0001] The invention discloses a method for separating and purifying dendrobium officinale polysaccharides, in particular to a method for separating and purifying dendrobium officinale polysaccharides through two-water phase extraction; it belongs to the technical field of extraction. Background technique [0002] Dendrobium officinale (Dendrobium officinale), as a traditional precious Chinese herbal medicine, is an edible plant with high medicinal value. Its main active ingredients are polysaccharides, alkaloids, flavonoids, polyphenols, trace elements and amino acids, among which The polysaccharide content is the highest, and the 2020 edition of "Chinese Pharmacopoeia" records that the polysaccharide content in Dendrobium officinale is not less than 25%. Dendrobium officinale polysaccharides have the effects of lowering blood sugar, protecting the liver, inhibiting bacteria, anti-tumor, anti-osteoporosis, protecting the stomach, anti-oxidation and enhancing...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 张小辉卫梦尧丁金龙
Owner GUANGDONG UNIV OF TECH
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