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Technique for extracting gentiopicroside from flower of gentiana straminea maxim

A technology of gentiopicroside and white flower gentian, applied in the direction of preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., to achieve the effect of easy industrial production

Active Publication Date: 2009-03-18
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report about the extraction of gentiopicroside from Gentiana japonica

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: a kind of technique of extracting gentiopicroside from the white flower gentian flower, comprises the following steps:

[0025] ① Pulverize 25kg of dried gentian flowers to 20 mesh;

[0026] ②Use 125L, 50% ethanol solution to cold soak Gentiana fragrans at room temperature for 4 times, each time for 6 hours, filter through a 200-mesh filter cloth, and obtain the filtrate after filtration;

[0027] ③ Combine the four filtrates, concentrate under reduced pressure below 40°C with a 20L rotary evaporator produced by Jiangsu Xinghai Instrument Equipment Co., Ltd., and obtain a concentrated solution after concentration. Utilize high performance liquid chromatography HPLC (source: U.S. Agilent Company, Agilent1100) to the concentrated solution to measure the content of gentiopicroside in Gentiana japonica Flos to be 6.28mg / g;

[0028] ④ First, soak the D-101 macroporous adsorption resin produced by Nankai University Chemical Factory in 5% NaOH solution and 5% HC...

Embodiment 2

[0030] Embodiment 2: a kind of technology that extracts gentiopicroside from the white flower gentian flower, comprises the following steps:

[0031] 1. 5kg of dried white flower gentian is crushed to 60 mesh;

[0032] ②Use 75L, 95% ethanol solution to cold-extract Gentiana japonica once at room temperature for 18 hours, filter through a 200-mesh filter cloth, and obtain the filtrate after filtration;

[0033] ③Concentrate the obtained filtrate under reduced pressure below 40°C with a 20L rotary evaporator to obtain a concentrated solution. Utilizing HPLC to measure the content of gentiopicroside in Gentiana japonica of the concentrated solution is 6.06mg / g;

[0034] ④Absorb the 3.5L concentrated solution with the treated D-101 macroporous adsorption resin, first elute with distilled water until the eluate is clear and colorless, then elute with a 60% ethanol solution, and detect by HPLC until the eluate is clear and colorless. There is no gentiopicroside in the dehydration;...

Embodiment 3

[0036] Embodiment 3: a kind of technology that extracts gentiopicroside from the white flower gentian flower, comprises the following steps:

[0037] ①Crush 1kg of dried white gentian flowers to 80 mesh;

[0038] ②Use 30L, 60% ethanol solution at room temperature to extract Gentiana radix by cold soaking twice, each time for 24 hours, and filter through a 200-mesh filter cloth to obtain the filtrate;

[0039] ③ Combine the filtrates obtained twice, and concentrate under reduced pressure below 40°C with a 20L rotary evaporator to obtain a concentrated solution. Utilize HPLC to measure the content of gentiopicroside in Gentiana japonica of the concentrated solution to be 6.38mg / g;

[0040] ④Absorb 4L of the concentrated solution with the treated D-101 macroporous adsorption resin, first elute with distilled water until the eluate is clear and colorless, then elute with 90% ethanol solution, and detect by HPLC until elution There is no gentiopicroside in the solution;

[0041]...

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PUM

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Abstract

The invention relates to a process for extracting gentiopicrin from flowers of gentiana straminea. The process is as follows: after the dried flower of gentiana straminea is crushed, cold maceration and extraction is carried out at room temperature taking alcohol as an extraction solvent, and gentiopicrin with higher purity is obtained through concentration, absorption and drying. The invention makes a study of the extraction and preparation process of gentiopicrin in gentiana straminea for the first time, determines the optimal cold maceration and extraction conditions and the purification process of the gentiopicrin in the gentiana straminea, and meets the requirements of developing various medicines taking gentiana straminea as main raw materials.

Description

technical field [0001] The invention relates to a method for extracting gentiopicroside, in particular to a process for extracting gentiopicroside from white gentian flowers. Background technique [0002] White-flowered gentian is the folk name of Gentiana straminea Maxim., a plant of the genus Gentiana Toum L. in the family Gentianaceae, and it is called "Xiejigabao" in Tibetan medicine. One of them has a medicinal history of 2,000 years. Twist Gentiana is also known as Gentiana Xiqin, Gentiana Daqin, Gentiana Zuoqin, Gentiana Zuotwister, etc. The folks call it white flower gentian because of its white flower color. As a commonly used traditional Chinese medicine, it has been recorded in successive dynasties of herbal medicines. It was first recorded in "Shen Nong's Materia Medica" and listed as a Chinese medicine. The main components of its roots are secoiridoid glycosides: gentiopicroside, picroside , as a drug glycoside, has a variety of medicinal effects - such as dis...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08
Inventor 索有瑞杨芳王洪伦
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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